| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,827,657 | G→A | 5.3% | A190A (GCG→GCA) | mtgA → | monofunctional biosynthetic peptidoglycan transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,827,657 | 0 | G | A | 5.3% | 284.9 / 3.0 | 93 | A190A (GCG→GCA) | mtgA | monofunctional biosynthetic peptidoglycan transglycosylase |
| Reads supporting (aligned to +/- strand): ref base G (45/43); new base A (2/3); total (47/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01 | |||||||||||
TTCTGGTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAG > NC_002947/5827511‑5827804 | ttCTGGTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTT‑‑GCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtg < 7:360203/148‑1 (MQ=255) gTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGCCCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtgct < 1:325926/148‑1 (MQ=255) gTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtgctgc > 1:83184/1‑149 (MQ=255) gTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtgct < 7:139281/147‑1 (MQ=255) gAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCaa < 1:426576/149‑1 (MQ=255) gAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCACCGCTGCCa < 2:292488/148‑1 (MQ=255) aaGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAg > 6:89310/1‑149 (MQ=255) aGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGc < 3:175465/149‑1 (MQ=255) aGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGc > 2:32462/1‑149 (MQ=255) ggAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGcc < 3:74857/149‑1 (MQ=255) gAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGcc > 2:126087/1‑148 (MQ=255) aaCGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCa < 8:55878/149‑1 (MQ=255) cGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAAt > 2:196241/1‑149 (MQ=255) ggATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCAAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAAt > 6:193705/1‑148 (MQ=255) ggATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGA‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATc < 2:35378/149‑1 (MQ=255) tctcGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCCGCCAAGCCCAATCAAGt > 6:373340/1‑149 (MQ=255) ctcGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGt < 5:193705/148‑1 (MQ=255) ctcGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGt < 8:187254/148‑1 (MQ=255) tcGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTg > 4:207574/1‑148 (MQ=255) cGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTg > 6:162581/1‑147 (MQ=255) gAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑g < 7:387526/149‑1 (MQ=255) gAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑g > 1:279744/1‑149 (MQ=255) aGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTAGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCACCGCTGCCagctgc > 1:79144/1‑132 (MQ=255) aGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑Gt < 3:207574/149‑1 (MQ=255) aGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCACCGCTGCCagctgc < 2:79144/136‑6 (MQ=255) tATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAg > 5:339811/1‑149 (MQ=255) tttGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCa < 3:78043/146‑1 (MQ=255) cAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGccgacc > 7:8320/1‑147 (MQ=255) aGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAg > 1:21644/1‑149 (MQ=255) aGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCACCGCTGCCag < 7:279416/118‑2 (MQ=255) aGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCACCGCTGCCag > 8:279416/1‑117 (MQ=255) cgcCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTg > 2:318142/1‑149 (MQ=255) ccGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGTCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGc > 7:167861/1‑148 (MQ=255) ccGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGcc > 5:45253/1‑149 (MQ=255) aGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTAc < 1:196241/149‑1 (MQ=255) ggCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGc < 2:97593/149‑1 (MQ=255) gCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGcc > 5:55360/1‑149 (MQ=255) aGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgc > 4:81570/1‑149 (MQ=255) gCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccgg < 8:8320/149‑1 (MQ=255) gCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccgg < 6:55360/149‑1 (MQ=255) cGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccggg > 4:334794/1‑149 (MQ=255) gtTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccgggccg > 7:112012/1‑149 (MQ=255) gtTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccgggccg > 7:228703/1‑149 (MQ=255) tttGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtgct > 3:6382/1‑89 (MQ=255) tttGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtgct < 4:6382/89‑1 (MQ=255) tttGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccgggccgg > 2:188457/1‑149 (MQ=255) tttGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccgggccgg < 6:21202/149‑1 (MQ=255) gTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTg > 4:413725/1‑148 (MQ=255) gTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTg > 6:382910/1‑148 (MQ=255) gCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGAt > 3:15347/1‑149 (MQ=255) aGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGc > 5:94479/1‑149 (MQ=255) cgTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTc < 4:391821/136‑1 (MQ=255) cgTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTc > 3:391821/1‑136 (MQ=255) gTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGACTGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGa < 1:126087/149‑1 (MQ=255) gTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGACAGATGAGc < 2:289269/149‑1 (MQ=255) aCTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCt < 6:339811/148‑1 (MQ=255) aCTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTg > 4:14010/1‑149 (MQ=255) cTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgg > 5:275492/1‑149 (MQ=255) cTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgg > 2:329343/1‑149 (MQ=255) tttGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTggg > 8:139477/1‑149 (MQ=255) tGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgggc < 4:15347/148‑1 (MQ=255) ggCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgggcg < 3:14010/148‑1 (MQ=255) gCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgggcgg < 8:112012/148‑1 (MQ=255) cG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgggcgggc < 2:83184/149‑1 (MQ=255) g‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGcc < 3:81570/149‑1 (MQ=255) g‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGcc > 3:131886/1‑149 (MQ=255) cGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCa < 3:334794/149‑1 (MQ=255) gACGCCAGCCGGCTCAGCCGCTAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGa > 5:160379/1‑130 (MQ=255) gACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCa < 5:89310/148‑1 (MQ=255) gCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCCGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCCCGCCCAGCtg < 5:373340/149‑2 (MQ=255) cAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTAcc < 6:275492/149‑1 (MQ=255) aGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCt > 3:138466/1‑149 (MQ=255) gCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTg < 8:228703/149‑1 (MQ=255) ccGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGTCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGa < 8:167861/149‑1 (MQ=255) ggCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATg < 6:45253/149‑1 (MQ=255) tCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAg > 6:31113/1‑149 (MQ=255) ccgccAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTg > 4:427382/1‑148 (MQ=255) ccgccAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTg > 6:101344/1‑148 (MQ=255) cgccAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGa > 5:398155/1‑149 (MQ=255) gccAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGAc < 4:327943/149‑1 (MQ=255) ccAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGAct > 7:93502/1‑149 (MQ=255) caggcagCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGActcctc < 7:139477/149‑1 (MQ=255) aggcagCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGTCCAGCTACCTGATGCAGCTGGACtcctcc < 1:32462/149‑1 (MQ=255) aggcagCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGActcctc > 4:209363/1‑148 (MQ=255) ggcagCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGActcctc > 5:348658/1‑147 (MQ=255) cagcgcagcTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGa < 1:118358/149‑1 (MQ=255) gcgcagcTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATAAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGa > 3:31624/1‑147 (MQ=255) gcagcTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGc > 3:407609/1‑149 (MQ=255) agcTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCtt > 2:234974/1‑149 (MQ=255) agcTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCtt < 1:269475/149‑1 (MQ=255) cTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTg < 3:413725/149‑1 (MQ=255) aGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGc < 5:101344/149‑1 (MQ=255) cGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAg < 1:318142/149‑1 (MQ=255) | TTCTGGTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAG > NC_002947/5827511‑5827804 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |