Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,827,659	T→C	5.5%	V191A (GTG→GCG)	mtgA →	monofunctional biosynthetic peptidoglycan transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,827,659	0	T	C	5.5%	266.1 / 3.8	90	V191A (GTG→GCG)	mtgA	monofunctional biosynthetic peptidoglycan transglycosylase
Reads supporting (aligned to +/- strand):  ref base T (45/40);  new base C (2/3);  total (47/43)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TTCTGGTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGC  >  NC_002947/5827511‑5827806                                                                                                                                                       |                                                                                                                                                      ttCTGGTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTT‑‑GCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtg                                                                                                                                                      <  7:360203/148‑1 (MQ=255)      gTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCGCCCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtgct                                                                                                                                                    <  1:325926/148‑1 (MQ=255)      gTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtgctgc                                                                                                                                                  >  1:83184/1‑149 (MQ=255)      gTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtgct                                                                                                                                                    <  7:139281/147‑1 (MQ=255)         gAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCaa                                                                                                                                               <  1:426576/149‑1 (MQ=255)         gAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCACCGCTGCCa                                                                                                                                                <  2:292488/148‑1 (MQ=255)          aaGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAg                                                                                                                                              >  6:89310/1‑149 (MQ=255)           aGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGc                                                                                                                                             <  3:175465/149‑1 (MQ=255)           aGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGc                                                                                                                                             >  2:32462/1‑149 (MQ=255)            ggAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGcc                                                                                                                                            <  3:74857/149‑1 (MQ=255)             gAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGcc                                                                                                                                            >  2:126087/1‑148 (MQ=255)              aaCGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCa                                                                                                                                          <  8:55878/149‑1 (MQ=255)                cGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAAt                                                                                                                                        >  2:196241/1‑149 (MQ=255)                 ggATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCAAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAAt                                                                                                                                        >  6:193705/1‑148 (MQ=255)                 ggATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGA‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATc                                                                                                                                       <  2:35378/149‑1 (MQ=255)                     tctcGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCCGCCAAGCCCAATCAAGt                                                                                                                                   >  6:373340/1‑149 (MQ=255)                      ctcGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGt                                                                                                                                   <  5:193705/148‑1 (MQ=255)                      ctcGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGt                                                                                                                                   <  8:187254/148‑1 (MQ=255)                       tcGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTg                                                                                                                                  >  4:207574/1‑148 (MQ=255)                        cGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTg                                                                                                                                  >  6:162581/1‑147 (MQ=255)                         gAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑g                                                                                                                             >  1:279744/1‑149 (MQ=255)                         gAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑g                                                                                                                             <  7:387526/149‑1 (MQ=255)                          aGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGTAGTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCACCGCTGCCagctgc                                                                                                                                           >  1:79144/1‑132 (MQ=255)                          aGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑Gt                                                                                                                            <  3:207574/149‑1 (MQ=255)                          aGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCACCGCTGCCagctgc                                                                                                                                           <  2:79144/136‑6 (MQ=255)                               tATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAg                                                                                                                       >  5:339811/1‑149 (MQ=255)                                 tttGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCa                                                                                                                        <  3:78043/146‑1 (MQ=255)                                       cAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGccgacc                                                                                                                 >  7:8320/1‑147 (MQ=255)                                        aGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAg                                                                                                              >  1:21644/1‑149 (MQ=255)                                        aGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCACCGCTGCCag                                                                                                                                               <  7:279416/118‑2 (MQ=255)                                        aGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCACCGCTGCCag                                                                                                                                               >  8:279416/1‑117 (MQ=255)                                          cgcCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTg                                                                                                            >  2:318142/1‑149 (MQ=255)                                            ccGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGTCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGc                                                                                                           >  7:167861/1‑148 (MQ=255)                                            ccGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGcc                                                                                                          >  5:45253/1‑149 (MQ=255)                                               aGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTAc                                                                                                       <  1:196241/149‑1 (MQ=255)                                                    ggCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGc                                                                                                  <  2:97593/149‑1 (MQ=255)                                                     gCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGcc                                                                                                 >  5:55360/1‑149 (MQ=255)                                                        aGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgc                                                                                              >  4:81570/1‑149 (MQ=255)                                                           gCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccgg                                                                                           <  8:8320/149‑1 (MQ=255)                                                           gCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccgg                                                                                           <  6:55360/149‑1 (MQ=255)                                                            cGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccggg                                                                                          >  4:334794/1‑149 (MQ=255)                                                                gtTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccgggccg                                                                                       >  7:228703/1‑149 (MQ=255)                                                                gtTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccgggccg                                                                                       >  7:112012/1‑149 (MQ=255)                                                                 tttGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtgct                                                                                                                                                    >  3:6382/1‑89 (MQ=255)                                                                 tttGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGtgct                                                                                                                                                    <  4:6382/89‑1 (MQ=255)                                                                 tttGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccgggccgg                                                                                      <  6:21202/149‑1 (MQ=255)                                                                 tttGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAgccgggccgg                                                                                      >  2:188457/1‑149 (MQ=255)                                                                     gTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTg                                                                                   >  6:382910/1‑148 (MQ=255)                                                                     gTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTg                                                                                   >  4:413725/1‑148 (MQ=255)                                                                       gCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGAt                                                                                >  3:15347/1‑149 (MQ=255)                                                                           aGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGc                                                                            >  5:94479/1‑149 (MQ=255)                                                                                      cgTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTc                                                                              <  4:391821/136‑1 (MQ=255)                                                                                      cgTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTc                                                                              >  3:391821/1‑136 (MQ=255)                                                                                       gTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGACTGTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGa                                                                  <  1:126087/149‑1 (MQ=255)                                                                                       gTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGACAGATGAGc                                                                <  2:289269/149‑1 (MQ=255)                                                                                             aCTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCt                                                           <  6:339811/148‑1 (MQ=255)                                                                                             aCTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTg                                                          >  4:14010/1‑149 (MQ=255)                                                                                              cTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgg                                                         >  5:275492/1‑149 (MQ=255)                                                                                              cTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgg                                                         >  2:329343/1‑149 (MQ=255)                                                                                               tttGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTggg                                                        >  8:139477/1‑149 (MQ=255)                                                                                                 tGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgggc                                                       <  4:15347/148‑1 (MQ=255)                                                                                                  ggCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgggcg                                                      <  3:14010/148‑1 (MQ=255)                                                                                                   gCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgggcgg                                                     <  8:112012/148‑1 (MQ=255)                                                                                                    cG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTgggcgggc                                                   <  2:83184/149‑1 (MQ=255)                                                                                                     g‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGcc                                                  >  3:131886/1‑149 (MQ=255)                                                                                                     g‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGcc                                                  <  3:81570/149‑1 (MQ=255)                                                                                                        cGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCa                                                <  3:334794/149‑1 (MQ=255)                                                                                                         gACGCCAGCCGGCTCAGCCGCTAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGa                                                                  >  5:160379/1‑130 (MQ=255)                                                                                                         gACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCa                                                <  5:89310/148‑1 (MQ=255)                                                                                                            gCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCCGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCCCGCCCAGCtg                                            <  5:373340/149‑2 (MQ=255)                                                                                                              cAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTAcc                                          <  6:275492/149‑1 (MQ=255)                                                                                                               aGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCt                                         >  3:138466/1‑149 (MQ=255)                                                                                                                gCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTg                                        <  8:228703/149‑1 (MQ=255)                                                                                                                 ccGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGTCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGa                                       <  8:167861/149‑1 (MQ=255)                                                                                                                   ggCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATg                                     <  6:45253/149‑1 (MQ=255)                                                                                                                      tCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAg                                  >  6:31113/1‑149 (MQ=255)                                                                                                                          ccgccAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTg                               >  4:427382/1‑148 (MQ=255)                                                                                                                          ccgccAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTg                               >  6:101344/1‑148 (MQ=255)                                                                                                                           cgccAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGa                             >  5:398155/1‑149 (MQ=255)                                                                                                                            gccAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGAc                            <  4:327943/149‑1 (MQ=255)                                                                                                                             ccAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGAct                           >  7:93502/1‑149 (MQ=255)                                                                                                                                 caggcagCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGActcctc                       <  7:139477/149‑1 (MQ=255)                                                                                                                                  aggcagCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGTCCAGCTACCTGATGCAGCTGGACtcctcc                      <  1:32462/149‑1 (MQ=255)                                                                                                                                  aggcagCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGActcctc                       >  4:209363/1‑148 (MQ=255)                                                                                                                                   ggcagCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGActcctc                       >  5:348658/1‑147 (MQ=255)                                                                                                                                     cagcgcagcTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGa                   <  1:118358/149‑1 (MQ=255)                                                                                                                                       gcgcagcTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATAAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGa                   >  3:31624/1‑147 (MQ=255)                                                                                                                                         gcagcTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGc               >  3:407609/1‑149 (MQ=255)                                                                                                                                           agcTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCtt             >  2:234974/1‑149 (MQ=255)                                                                                                                                           agcTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCtt             <  1:269475/149‑1 (MQ=255)                                                                                                                                             cTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTg           <  3:413725/149‑1 (MQ=255)                                                                                                                                                  aGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGc      <  5:101344/149‑1 (MQ=255)                                                                                                                                                    cGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAg    <  1:318142/149‑1 (MQ=255)                                                                                                                                                      gTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCCGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAggc  <  8:93502/149‑1 (MQ=255)                                                                                                                                                      gTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAggc  >  7:4359/1‑149 (MQ=255)                                                                                                                                                      gTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAggc  >  5:75104/1‑149 (MQ=255)                                                                                                                                                       |                                                                                                                                                      TTCTGGTCGAAGGAACGGATTCTCGAGGTCTATTTGAACAGCGCCGAGTGGGGCAAGGGCGT‑GTTTGGTGCCCAGGCAGCGGCGCGTTATCACTTTGGCG‑TCGACGCCAGCCGGCTCAGCCGCCAGCAGGCAGCGCAGCTGGCAGCGGTGCTGCCAAGCCCAATCAAGTGGA‑‑GTGCCAGCCGACCGAGTGCCTACGTGGCCAGCCGGGCCGGCTGGATTCGCCGGCAGATGAGCCAGCTGGGCGGGCCCAGCTACCTGATGCAGCTGGACTCCTCCAGAAAGCTTTGAATGCAGGC  >  NC_002947/5827511‑5827806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑