Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,866,696 G→T 8.5% intergenic (+19/+38) PP_5140 → / ← thyA MerR family transcriptional regulator/thymidylate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,866,6960GT 8.5% 273.0 / 10.6 91intergenic (+19/+38)PP_5140/thyAMerR family transcriptional regulator/thymidylate synthase
Reads supporting (aligned to +/- strand):  ref base G (42/41);  new base T (4/4);  total (46/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGAGCTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGTTTCG  >  NC_002947/5866549‑5866842
                                                                                                                                                    |                                                                                                                                                  
gtggAGCTGCGGAGTCGCTGCAACGCGAAGGGGAGTGAATGCGCGATCTTGCAGAAACTGGAGACAAAGGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTg                                                                                                                                                    <  8:379270/148‑1 (MQ=255)
   gAGCTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAccc                                                                                                                                                <  6:354666/149‑1 (MQ=255)
     gCTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCcg                                                                                                                                              <  4:174767/149‑1 (MQ=255)
     gCTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCcg                                                                                                                                              >  6:16533/1‑149 (MQ=255)
      cTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTTCACCcgc                                                                                                                                             >  5:181130/1‑149 (MQ=255)
        gcggcggcgCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCcgcg                                                                                                                                            >  5:72729/1‑148 (MQ=255)
         cggcggcgCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCcgc                                                                                                                                             <  4:331784/146‑1 (MQ=255)
         cggcggcgCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCcgc                                                                                                                                             >  3:331784/1‑146 (MQ=255)
            cggcgCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTTCACCCGCGAagct                                                                                                                                       <  1:387218/149‑3 (MQ=255)
            cggcgCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTCCAGCAACTGGAGACAAACTGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAagag                                                                                                                                       <  4:34280/149‑1 (MQ=255)
            cggcgCTACAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAagag                                                                                                                                       >  7:65966/1‑149 (MQ=255)
             ggcgCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAgg                                                                                                                                      >  3:245937/1‑149 (MQ=255)
              gcgcTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTTCACCCGCGAagcttt                                                                                                                                     <  3:54104/149‑5 (MQ=255)
                gcTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGTAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCa                                                                                                                                   <  5:171258/149‑1 (MQ=255)
                gcTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTTCACCCGCGAagcttttg                                                                                                                                   <  6:181130/149‑7 (MQ=255)
                gcTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTTCACCCGCGAagctttt                                                                                                                                    <  6:216148/148‑6 (MQ=255)
                gcTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTTCACCCGCGAagctttt                                                                                                                                    >  5:216148/1‑143 (MQ=255)
                   gCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGtt                                                                                                                                <  3:11086/149‑1 (MQ=255)
                    cAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTc                                                                                                                               >  1:170163/1‑149 (MQ=255)
                    cAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTc                                                                                                                               >  8:163106/1‑149 (MQ=255)
                    cAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTc                                                                                                                               >  1:260322/1‑149 (MQ=255)
                     aaCGCGCAGGGGAGGTAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCt                                                                                                                              <  8:271032/149‑1 (MQ=255)
                       cgcgCAGGGGATTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTaa                                                                                                                            <  6:383576/149‑1 (MQ=255)
                              gggAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATaga                                                                                                                     <  2:260322/149‑1 (MQ=255)
                              gggAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATaga                                                                                                                     <  1:26208/149‑1 (MQ=255)
                                gAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATa                                                                                                                       >  6:423136/1‑145 (MQ=255)
                                 aGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGAt                                                                                                                  <  7:94003/149‑1 (MQ=255)
                                 aGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGAt                                                                                                                  <  8:65966/149‑1 (MQ=255)
                                  gTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATc                                                                                                                 >  6:308703/1‑149 (MQ=255)
                                      aTGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTAt                                                                                                              <  5:230058/148‑1 (MQ=255)
                                       tGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCa                                                                                                            <  1:127432/149‑1 (MQ=255)
                                        gcgcGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCAATGAGCCAAAAGCTTCGCGGGTGAACCCGCGaa                                                                                                                                          >  4:48464/1‑118 (MQ=255)
                                        gcgcGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCAATGAGCCAAAAGCTTCGCGGGTGAACCCGCGaa                                                                                                                                          <  3:48464/118‑1 (MQ=255)
                                         cgcgATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAg                                                                                                           <  2:170163/148‑1 (MQ=255)
                                                  gcagcaACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGc                                                                                                      <  5:16533/144‑1 (MQ=255)
                                                   cagcaACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCACATGTGGGGCGGAGTCACGGTCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCg                                                                                                 <  5:313381/148‑1 (MQ=255)
                                                            gagaCAAACGGTGCGGTAT‑CGGTACCGGATACCGAAAATTCCCATGTGGGGCTGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAAGAGAGAGATCTCAGACCGCAATCTGCGCTGTCa                                                                                        >  4:225449/1‑148 (MQ=255)
                                                            gagaCAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGAcc                                                                                                        <  3:59707/132‑1 (MQ=255)
                                                            gagaCAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGAcc                                                                                                        >  4:59707/1‑132 (MQ=255)
                                                            gagaCAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTc                                                                                         <  2:32907/147‑1 (MQ=255)
                                                               aCAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCCGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGCCCGCCATCGCCGCTGTCATCgg                                                                                    >  6:337467/1‑149 (MQ=255)
                                                                 aaaCGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGgcg                                                                                  >  8:225431/1‑149 (MQ=255)
                                                                      gggCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATgg                                                                             >  4:18568/1‑149 (MQ=255)
                                                                      gggCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATgg                                                                             <  7:163106/149‑1 (MQ=255)
                                                                      gggCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATgg                                                                             >  7:163875/1‑149 (MQ=255)
                                                                        tcGGTATCCGGTACC‑GATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTg                                                                           >  5:323568/2‑149 (MQ=255)
                                                                         cGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGc                                                                          <  5:308703/149‑1 (MQ=255)
                                                                            tAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCa                                                                       >  1:211133/1‑149 (MQ=255)
                                                                            gtt‑CGGTATCCGGTACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCt                                                                                            <  6:74446/126‑1 (MQ=255)
                                                                            gtt‑CGGTATCCGGTACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCt                                                                                            >  5:74446/3‑128 (MQ=255)
                                                                              t‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCa                                                                       >  4:334827/1‑147 (MQ=255)
                                                                                cGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAg                                                                    >  6:29501/1‑149 (MQ=255)
                                                                                  gTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCAt                                                                               <  7:247916/136‑1 (MQ=255)
                                                                                  gTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCAt                                                                               >  8:247916/1‑136 (MQ=255)
                                                                                  gTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGcc                                                                  >  6:279369/1‑149 (MQ=255)
                                                                                    aCCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTg                                                                           <  6:316376/138‑1 (MQ=255)
                                                                                    aCCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTg                                                                           >  5:316376/1‑138 (MQ=255)
                                                                                      cGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTcc                                                              <  5:29501/149‑1 (MQ=255)
                                                                                       ggATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTcca                                                             <  4:245937/149‑1 (MQ=255)
                                                                                         aTACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagc                                                           <  6:72729/149‑1 (MQ=255)
                                                                                            ccGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagc                                                           >  5:71951/1‑146 (MQ=255)
                                                                                             cGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagccag                                                        >  7:293068/1‑148 (MQ=255)
                                                                                             cGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACAGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagccag                                                        >  2:257164/1‑148 (MQ=255)
                                                                                               aaCATTCCCATGTGGGGGGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGaaa                                                     <  2:396482/149‑1 (MQ=255)
                                                                                               aaCATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGaaa                                                     >  7:60186/1‑149 (MQ=255)
                                                                                                aCATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGaaa                                                     >  2:335429/1‑148 (MQ=255)
                                                                                                   ttCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCAc                                                 <  6:348228/149‑1 (MQ=255)
                                                                                                     cccATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTc                                               >  5:296200/1‑149 (MQ=255)
                                                                                                      ccATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTc                                               <  8:163875/148‑1 (MQ=255)
                                                                                                          gtgGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCt                                           >  5:175431/1‑148 (MQ=255)
                                                                                                           tgGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTc                                          >  2:204583/1‑148 (MQ=255)
                                                                                                             gggCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAAt                                       <  6:296200/149‑1 (MQ=255)
                                                                                                             gggCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGGGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCaa                                        <  6:323568/148‑1 (MQ=255)
                                                                                                              ggCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAAtc                                      <  2:211133/149‑1 (MQ=255)
                                                                                                                  gAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAAtcttct                                  >  7:329124/1‑149 (MQ=255)
                                                                                                                   aGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTtctc                                 <  4:194231/149‑1 (MQ=255)
                                                                                                                              ttGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCg                      <  3:334827/149‑1 (MQ=255)
                                                                                                                                   ccAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCa                 >  8:356137/1‑149 (MQ=255)
                                                                                                                                    cAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCa                 >  4:119602/1‑148 (MQ=255)
                                                                                                                                     aaaaGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATa               <  7:225431/149‑1 (MQ=255)
                                                                                                                                      aaaGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATa               >  2:167686/1‑148 (MQ=255)
                                                                                                                                        ctcTTCGCGGGTTCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACtt            >  2:100102/3‑149 (MQ=255)
                                                                                                                                        ctcTTCGCGGGTTCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACtt            >  8:338806/3‑149 (MQ=255)
                                                                                                                                          cTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGc          <  1:45696/149‑1 (MQ=255)
                                                                                                                                          cTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGc          >  7:41977/1‑149 (MQ=255)
                                                                                                                                          cTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGc          >  1:417319/1‑149 (MQ=255)
                                                                                                                                                 ggTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATTGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGTTTc   <  5:423136/149‑1 (MQ=255)
                                                                                                                                                 ggTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCa                 <  7:61171/135‑1 (MQ=255)
                                                                                                                                                 ggTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCa                 >  8:61171/1‑135 (MQ=255)
                                                                                                                                                  gTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATAc              <  5:88676/137‑1 (MQ=255)
                                                                                                                                                  gTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATAc              >  6:88676/1‑137 (MQ=255)
                                                                                                                                                  gTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGTTTCg  >  3:212175/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GTGGAGCTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTAT‑CGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGTTTCG  >  NC_002947/5866549‑5866842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑