Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,869,113	T→G	5.2%	T84P (ACC→CCC)	PP_5143 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,869,113	0	T	G	5.2%	282.7 / 4.0	90	T84P (ACC→CCC)	PP_5143	membrane protein
Reads supporting (aligned to +/- strand):  ref base T (35/50);  new base G (3/2);  total (38/52)
Fisher's exact test for biased strand distribution p-value = 6.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACGGCTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGAAGCGTCGAAACCCTGCAGGGTAAAGC  >  NC_002947/5868968‑5869259                                                                                                                                                   |                                                                                                                                                   ccACGGCTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCAt                                                                                                                                                 >  7:153209/1‑149 (MQ=255)  cACGGCTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGTCCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATc                                                                                                                                                >  1:314435/1‑149 (MQ=255)  cACGGCTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGTCCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATc                                                                                                                                                >  1:320276/1‑149 (MQ=255)  cACGGCTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGgtcgtc                                                                                                                                                <  1:167756/149‑1 (MQ=255)  cACGGCTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATc                                                                                                                                                >  3:296131/1‑149 (MQ=255)  cACGGCTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATc                                                                                                                                                >  2:370339/1‑149 (MQ=255)   aCGGCTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATcc                                                                                                                                               <  5:183234/149‑1 (MQ=255)   aCGGCTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATcc                                                                                                                                               <  5:194459/149‑1 (MQ=255)   aCGGCTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATc                                                                                                                                                >  6:12876/1‑148 (MQ=255)   aCGGCTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGAAGGTCATc                                                                                                                                                >  2:366922/1‑148 (MQ=255)       cTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCTAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGGGGATGCCGATGGTCATCCAGAt                                                                                                                                           <  7:274177/149‑1 (MQ=255)       cTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGAt                                                                                                                                           <  2:320276/149‑1 (MQ=255)       cTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGAt                                                                                                                                           <  2:314435/149‑1 (MQ=255)        tGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGAt                                                                                                                                           >  2:44827/1‑148 (MQ=255)          gCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGa                                                                                                                                         <  4:268140/148‑1 (MQ=255)           ccTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATg                                                                                                                                          >  6:103919/1‑146 (MQ=255)            cTTGGGTTTGAGGTCAAGGGCCATCTGCGCGGGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATg                                                                                                                                          >  1:260862/1‑146 (MQ=255)              tGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATg                                                                                                                                          >  1:96389/1‑143 (MQ=255)                ggTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCg                                                                                                                                   <  1:222971/148‑1 (MQ=255)                 gTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGa                                                                                                                                         <  4:345989/141‑1 (MQ=255)                 gTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGa                                                                                                                                         >  3:345989/1‑141 (MQ=255)                 gTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGc                                                                                                                                 <  8:41844/149‑1 (MQ=255)                  tttGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGc                                                                                                                                 >  5:332596/1‑148 (MQ=255)                     gAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCAt                                                                                                                              <  5:196582/148‑1 (MQ=255)                       ggTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTg                                                                                                                            >  2:296559/1‑148 (MQ=255)                        gTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCa                                                                                                                          <  3:101801/149‑1 (MQ=255)                         tCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTc                                                                                                                                                   <  6:233994/123‑1 (MQ=255)                         tCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTc                                                                                                                                                   >  5:233994/1‑123 (MQ=255)                            aGGGCGATCTGCGC‑GGCTATGACCAGGGCTAACACAGCGATGATCTTTTGCAGCAGTGGCGCCTGGATCTGCGAAGGGGTCTTGGCACCGACACCGGCCGCCTGGAGTATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACcgc                                                                                                                      <  8:153209/149‑1 (MQ=255)                            aGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACcgc                                                                                                                      <  5:12876/149‑1 (MQ=255)                             gggCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACcgcg                                                                                                                     >  1:218384/1‑149 (MQ=255)                                ccATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCgcgcgc                                                                                                                  >  5:325392/1‑149 (MQ=255)                                       cgc‑gGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGc                                                                                                              <  1:328730/146‑1 (MQ=255)                                       cgc‑gGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGc                                                                                                              <  5:103919/146‑1 (MQ=255)                                       cgc‑gGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCg                                                                                                             <  7:18388/147‑1 (MQ=255)                                        gc‑ggcgATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCggtg                                                                                                          <  1:44827/149‑1 (MQ=255)                                        gc‑ggcgATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCggtg                                                                                                          <  4:387000/149‑1 (MQ=255)                                           ggcgATGCCCAGGGCGAACACACGGATGATGTTTTGGAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGCAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCggtggt                                                                                                        <  8:95941/149‑1 (MQ=255)                                            gcgATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGgtggtg                                                                                                       <  1:222236/149‑1 (MQ=255)                                              gATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACcgcg                                                                                                                     <  6:236966/133‑1 (MQ=255)                                              gATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACcgcg                                                                                                                     >  5:236966/1‑133 (MQ=255)                                                   ccAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCg                                                                                                 >  1:423613/1‑148 (MQ=255)                                                   ccAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCg                                                                                                 >  5:115206/1‑148 (MQ=255)                                                   ccAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGc                                                                                                <  8:333511/149‑1 (MQ=255)                                                       ggCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCTAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACgg                                                                                            <  4:18285/149‑1 (MQ=255)                                                        gCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGc                                                                                           <  6:332596/149‑1 (MQ=255)                                                        gCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGc                                                                                           >  1:398323/1‑149 (MQ=255)                                                                acCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATc                                                                                    >  7:198825/1‑148 (MQ=255)                                                                acCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCg                                                                                   <  7:341095/149‑1 (MQ=255)                                                                  cGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGaa                                                                                 <  7:48592/149‑1 (MQ=255)                                                                  cGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGaa                                                                                 <  3:98696/149‑1 (MQ=255)                                                                  cGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGaa                                                                                 <  6:325392/149‑1 (MQ=255)                                                                   gatgatCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGATGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAg                                                                                >  7:309800/1‑149 (MQ=255)                                                                   gatgatCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAg                                                                                <  2:218384/149‑1 (MQ=255)                                                                       atCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGa                                                                             <  8:198825/148‑1 (MQ=255)                                                                          ttttGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGAc                                                                         >  2:69188/1‑149 (MQ=255)                                                                           tttGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGt                                                                                         <  4:399029/132‑1 (MQ=255)                                                                           tttGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGt                                                                                         >  3:399029/1‑132 (MQ=255)                                                                           tttGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCAGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGAc                                                                         >  8:174950/1‑148 (MQ=255)                                                                            ttGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGa                                                                          <  1:296559/146‑1 (MQ=255)                                                                            ttGCAGCAGTGACCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAg                                                                           <  2:223828/145‑1 (MQ=255)                                                                               cagcagTGGCCCTTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGAtg                                                                     <  1:366922/148‑1 (MQ=255)                                                                               cagcagTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGAtg                                                                     <  2:260862/148‑1 (MQ=255)                                                                                agcagTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGAtggt                                                                   >  1:161448/1‑149 (MQ=255)                                                                                 gcagTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGAtggtg                                                                  <  4:10845/149‑1 (MQ=255)                                                                                   agTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGc                                                                <  2:161448/149‑1 (MQ=255)                                                                                   agTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGc                                                                <  5:393484/149‑1 (MQ=255)                                                                                       gCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCaaa                                                            <  7:81315/149‑1 (MQ=255)                                                                                          ccTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGAc                                                         >  5:313397/1‑149 (MQ=255)                                                                                              gATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGt                                                       <  5:444246/147‑1 (MQ=255)                                                                                               aTCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGcc                                                    >  3:130616/1‑149 (MQ=255)                                                                                                tCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCg                                                   >  5:365809/1‑149 (MQ=255)                                                                                                  aGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGAc                                                 >  8:67949/1‑149 (MQ=255)                                                                                                   gCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCAGCCAGATGAAGCTCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACg                                                >  5:300971/1‑149 (MQ=255)                                                                                                            tCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGGCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGa                                       <  4:2933/149‑1 (MQ=255)                                                                                                               tGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGt                                     <  2:12061/148‑1 (MQ=255)                                                                                                                       acacCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGa                            <  7:67949/149‑1 (MQ=255)                                                                                                                        cacCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGaa                           <  6:115206/149‑1 (MQ=255)                                                                                                                           cGGCCCCCAGGAGGATGCCGATGGTCATGCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGt                                         >  1:244586/1‑132 (MQ=255)                                                                                                                           cGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGt                                         <  2:244586/132‑1 (MQ=255)                                                                                                                           cGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGAAGCg                        <  7:174950/149‑1 (MQ=255)                                                                                                                              cccccAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGAAGCGTc                      >  3:424923/1‑148 (MQ=255)                                                                                                                               ccccAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGAAGCGTCg                     >  8:248289/1‑148 (MQ=255)                                                                                                                                cccAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGAAGCGTCGaa                   <  6:365809/149‑1 (MQ=255)                                                                                                                                    tctggaTGACCATCGGCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCTGCACGGAAGGGACGAAAccc               >  4:265581/4‑149 (MQ=255)                                                                                                                                     ctggaTGACCATCGGCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGAAGCGTCGAAAc                 >  6:65079/3‑146 (MQ=255)                                                                                                                                     ctggaTGACCATCGGCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGAAGCGTCGAAAc                 <  5:65079/144‑1 (MQ=255)                                                                                                                                     ctggaTGACCATCGGCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGAAGCGTCGAAACCCt              >  5:101963/3‑149 (MQ=255)                                                                                                                                          tGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGAAGCGTCGAAACCCTGCAgg         <  7:224516/149‑1 (MQ=255)                                                                                                                                                  gTCATCCAGATGTAGATCGGCCATTGCAGCGCGCGCGTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGAAGCGTCGAAACCCTGCAGGGTAAAGc  <  8:309800/148‑1 (MQ=255)                                                                                                                                                  gTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGAAGCGTCGAAACCCTGCAGGGTAAAGc  <  4:296131/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CCACGGCTGGCCTTGGGTTTGAGGTCAAGGGCCATCTGCGC‑GGCGATGACCAGGGCGAACACACCGATGATCTTTTGCAGCAGTGGCCCCTGGATCAGCGAAGCGGTCTTGGCACCGACACCGGCCCCCAGGAGGATGCCGATGGTCATCCAGATGAAGATCGGCCATTGCACCGCGCGCTTGCGGTGGTGCTCCCGCACGGCGTTAATCGAAGTGAAGACGATGGTGGCCAAAGACGTGCCGACGGCCAGGTGAGTCAGCACCGAAGCGTCGAAACCCTGCAGGGTAAAGC  >  NC_002947/5868968‑5869259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑