Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,902,946 G→A 5.4% P31S (CCT→TCT)  PP_5175 ← HlyD family secretion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,902,9460GA 5.4% 346.1 / 5.1 111P31S (CCT→TCT) PP_5175HlyD family secretion protein
Reads supporting (aligned to +/- strand):  ref base G (52/53);  new base A (4/2);  total (56/55)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 115 total aligned reads displayed.

ACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAG  >  NC_002947/5902798‑5903089
                                                                                                                                                    |                                                                                                                                               
aCGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAgg                                                                                                                                                 <  6:180946/149‑1 (MQ=255)
aCGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAgg                                                                                                                                                 <  3:238610/149‑1 (MQ=255)
aCGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCACCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAgg                                                                                                                                                 >  5:363193/1‑149 (MQ=255)
  gTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAgg                                                                                                                                                 >  2:189671/1‑147 (MQ=255)
      tCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAgtgt                                                                                                                                           >  6:125681/1‑149 (MQ=255)
      tCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAgtg                                                                                                                                            >  5:284403/1‑148 (MQ=255)
        gCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGtct                                                                                                                                         >  3:44754/1‑149 (MQ=255)
            aCGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCTGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCggg                                                                                                                                     >  1:356593/1‑149 (MQ=255)
            aCGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCggg                                                                                                                                     <  3:86740/149‑1 (MQ=255)
             cGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGACACTGCCTCgggt                                                                                                                                    <  5:208281/149‑2 (MQ=255)
              gTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGa                                                                                                                                   <  2:16578/149‑1 (MQ=255)
                 gACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACct                                                                                                                                >  5:131600/1‑149 (MQ=255)
                    gCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCtt                                                                                                                             >  3:63246/1‑149 (MQ=255)
                        cgcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCtt                                                                                                                         >  8:322646/1‑149 (MQ=255)
                         gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCg                                                                                                                                       >  1:13155/1‑134 (MQ=255)
                         gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCg                                                                                                                                       <  2:13155/134‑1 (MQ=255)
                         gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTg                                                                                                                        >  1:376278/1‑149 (MQ=255)
                         gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTg                                                                                                                        <  5:125681/149‑1 (MQ=255)
                         gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTg                                                                                                                        <  7:237154/149‑1 (MQ=255)
                         gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTCTCTCGGGGACCTCTTCCTTg                                                                                                                        >  6:379868/1‑149 (MQ=255)
                            aaaTGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTa                                                                                                                     <  8:339706/149‑1 (MQ=255)
                            aaaTGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTa                                                                                                                     >  5:347419/1‑149 (MQ=255)
                                  tCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCg                                                                                                               >  6:11947/1‑149 (MQ=255)
                                      gccgccGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCgg                                                                                                              <  6:347419/146‑1 (MQ=255)
                                        cgccgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTTGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGc                                                                                                         <  5:11455/149‑1 (MQ=255)
                                          ccgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCAt                                                                                                       <  2:356593/149‑1 (MQ=255)
                                           cgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATg                                                                                                      <  2:210102/149‑1 (MQ=255)
                                             aCGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTa                                                                                                    <  6:128428/149‑1 (MQ=255)
                                                 ggAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTacaac                                                                                                <  8:304729/149‑1 (MQ=255)
                                                  gAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTacaaca                                                                                               >  5:79312/1‑149 (MQ=255)
                                                  gAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTacaa                                                                                                 >  2:161096/1‑147 (MQ=255)
                                                  gAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTacaa                                                                                                 >  2:155123/1‑147 (MQ=255)
                                                       aCAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCATCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCTAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGGGAGCATGTACAACAGcccc                                                                                          <  5:124508/149‑1 (MQ=255)
                                                       aCAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGccc                                                                                           >  3:228736/1‑148 (MQ=255)
                                                       aCAAATCGGTCTGCACCCGCGCCTGCACGGCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGcccc                                                                                          <  6:357796/149‑1 (MQ=255)
                                                       aCAAATCGGGCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGcccc                                                                                          <  6:17389/149‑1 (MQ=255)
                                                           aTCGTTCTGCACCCGCGCCTTCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTTCTGCACACCCACCCGAGGCAGTGTATCGGGGACCTCTTCCTTTCTACAGCCGGTGAGCATTTACAACAGCCCCAGGc                                                                                      <  5:63108/149‑1 (MQ=255)
                                                           aTCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAgg                                                                                       >  2:325200/1‑148 (MQ=255)
                                                            tCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCa                                                                                     <  6:428343/149‑1 (MQ=255)
                                                                    cACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGTGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAAc                                                                               <  1:415462/147‑1 (MQ=255)
                                                                     aCCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACTGa                                                                            >  1:19024/1‑149 (MQ=255)
                                                                     aCCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGa                                                                            >  2:326981/1‑149 (MQ=255)
                                                                      cccGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGa                                                                            >  1:24827/1‑148 (MQ=255)
                                                                       ccGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAAcc                                                                              <  4:5470/145‑1 (MQ=255)
                                                                       ccGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAAcc                                                                              >  3:5470/1‑145 (MQ=255)
                                                                       ccGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGCTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGa                                                                            >  7:168124/1‑147 (MQ=255)
                                                                             cTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGAc                                                                    >  7:149971/1‑149 (MQ=255)
                                                                              tGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACg                                                                   >  6:162044/1‑149 (MQ=255)
                                                                               gCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACg                                                                   >  7:400387/1‑148 (MQ=255)
                                                                                cACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCa                                                                                     >  1:428565/1‑129 (MQ=255)
                                                                                cACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCa                                                                                     <  2:428565/129‑1 (MQ=255)
                                                                                 aCGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCAt                                                                >  8:222042/1‑149 (MQ=255)
                                                                                 aCGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCAt                                                                <  1:189671/149‑1 (MQ=255)
                                                                                  cGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCAt                                                                >  1:310400/1‑148 (MQ=255)
                                                                                   gTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGCCCTCTTCCTTGCTACAGCAGGTGAGCATGTACAACAGCCCCAGGCAGACAACCCACAGCGGACGCATc                                                               >  4:273665/1‑148 (MQ=255)
                                                                                   gTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATc                                                               >  5:53827/1‑148 (MQ=255)
                                                                                   gTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTGGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGTTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCa                                                              <  8:197290/149‑1 (MQ=255)
                                                                                      gCCGTTCAAAGTGACCCTGGCAGCGAAATCTGTTGGTTGTACATGCTGCACACCCACCCGAGGCAGTGTCTCGTGTACCTCTTCCTTGCTACAGCCGTTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAAc                                                            <  2:316355/148‑1 (MQ=255)
                                                                                       ccGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGc                                                          <  4:63246/149‑1 (MQ=255)
                                                                                             aaaGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGAGGACGCATCAACGCCAGGCt                                                    >  8:369688/1‑149 (MQ=255)
                                                                                               aGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTTCAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTgg                                                  <  1:326981/149‑1 (MQ=255)
                                                                                               aGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTgg                                                  <  2:310400/149‑1 (MQ=255)
                                                                                               aGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTgg                                                  >  7:248893/1‑149 (MQ=255)
                                                                                               aGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTgg                                                  <  6:284403/149‑1 (MQ=255)
                                                                                                 tGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGc                                                 <  8:400387/148‑1 (MQ=255)
                                                                                                   aCCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTcc                                              >  5:337830/1‑149 (MQ=255)
                                                                                                     ccTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCctt                                            >  3:179817/1‑149 (MQ=255)
                                                                                                       tGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCcttgc                                          >  3:87688/1‑149 (MQ=255)
                                                                                                         gCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCcttgctt                                        <  4:44754/149‑1 (MQ=255)
                                                                                                         gCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCcttgc                                          <  1:161096/147‑1 (MQ=255)
                                                                                                         gCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCcttgc                                          <  1:155123/147‑1 (MQ=255)
                                                                                                           aGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTtgt                                      <  6:53827/149‑1 (MQ=255)
                                                                                                           aGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTtgt                                      <  6:154688/149‑1 (MQ=255)
                                                                                                             cGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGc                                    <  5:162044/149‑1 (MQ=255)
                                                                                                                    ggTGTGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAAt                              <  6:417905/148‑1 (MQ=255)
                                                                                                                     gTGGGTTGTACCTGCTGCACACCCACGCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGa                            >  1:11677/1‑149 (MQ=255)
                                                                                                                      tGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGAt                           <  8:5404/149‑1 (MQ=255)
                                                                                                                         gTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTtg                        <  2:11677/149‑1 (MQ=255)
                                                                                                                         gTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTtg                        >  8:333836/1‑149 (MQ=255)
                                                                                                                          ttGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTtgt                       >  3:191109/1‑149 (MQ=255)
                                                                                                                            gTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTtgtg                      <  6:412331/148‑1 (MQ=255)
                                                                                                                               ccTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGAt                  >  7:64642/1‑149 (MQ=255)
                                                                                                                                  gctgcACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCa                                                        <  6:398038/108‑1 (MQ=255)
                                                                                                                                  gctgcACACCCACACGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCa                                                        >  5:398038/1‑108 (MQ=255)
                                                                                                                                   ctgcACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGa              >  1:178968/1‑149 (MQ=255)
                                                                                                                                     gcACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACt            >  7:28497/1‑149 (MQ=255)
                                                                                                                                      cacaCCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACt            <  4:191109/148‑1 (MQ=255)
                                                                                                                                       acacCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGAGCACAAATGATTTGTGGGATGCGACtgt          <  4:87688/149‑1 (MQ=255)
                                                                                                                                        cacccaccCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACtgtg         <  8:168124/149‑1 (MQ=255)
                                                                                                                                         acccacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACtgt          >  8:122423/1‑147 (MQ=255)
                                                                                                                                         acccacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACtgt          >  5:200794/1‑147 (MQ=255)
                                                                                                                                            cacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCg     >  4:131865/1‑149 (MQ=255)
                                                                                                                                             acccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACtgt          >  6:93856/1‑143 (MQ=255)
                                                                                                                                             acccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACtgt          <  5:93856/143‑1 (MQ=255)
                                                                                                                                             acccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCg     >  2:93878/1‑148 (MQ=255)
                                                                                                                                              cccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTa   <  2:24827/149‑1 (MQ=255)
                                                                                                                                              cccGAGACACTGCCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGCCAACCGCCAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGTTGCGACTGTGAGCg     >  5:271040/1‑147 (MQ=255)
                                                                                                                                              cccGAGACACTGCCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCg     >  3:136172/1‑147 (MQ=255)
                                                                                                                                              cccGAGACACTGCCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCg     >  5:245454/1‑147 (MQ=255)
                                                                                                                                               ccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAg  <  8:149971/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
ACGTGATCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAG  >  NC_002947/5902798‑5903089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑