Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,902,952 T→C 5.4% T29A (ACA→GCA)  PP_5175 ← HlyD family secretion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,902,9520TC 5.4% 327.8 / 5.7 111T29A (ACA→GCA) PP_5175HlyD family secretion protein
Reads supporting (aligned to +/- strand):  ref base T (51/54);  new base C (4/2);  total (55/56)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

TCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTC  >  NC_002947/5902804‑5903099
                                                                                                                                                    |                                                                                                                                                   
  gCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGtct                                                                                                                                                   >  3:44754/1‑149 (MQ=255)
      aCGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCTGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCggg                                                                                                                                               >  1:356593/1‑149 (MQ=255)
      aCGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCggg                                                                                                                                               <  3:86740/149‑1 (MQ=255)
       cGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGACACTGCCTCgggt                                                                                                                                              <  5:208281/149‑2 (MQ=255)
       cGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGACACTGCCTCgggt                                                                                                                                              <  8:31099/149‑2 (MQ=255)
        gTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGa                                                                                                                                             <  2:16578/149‑1 (MQ=255)
         tCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGAc                                                                                                                                            >  1:210102/1‑149 (MQ=255)
                  cgcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTcc                                                                                                                                     <  3:385428/147‑1 (MQ=255)
                  cgcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCtt                                                                                                                                   >  8:322646/1‑149 (MQ=255)
                   gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCg                                                                                                                                                 >  1:13155/1‑134 (MQ=255)
                   gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCg                                                                                                                                                 <  2:13155/134‑1 (MQ=255)
                   gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTg                                                                                                                                  >  1:376278/1‑149 (MQ=255)
                   gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTg                                                                                                                                  <  5:125681/149‑1 (MQ=255)
                   gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTg                                                                                                                                  <  7:237154/149‑1 (MQ=255)
                   gcgAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTCTCTCGGGGACCTCTTCCTTg                                                                                                                                  >  6:379868/1‑149 (MQ=255)
                      aaaTGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTa                                                                                                                               <  6:131600/149‑1 (MQ=255)
                      aaaTGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTa                                                                                                                               <  8:339706/149‑1 (MQ=255)
                      aaaTGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTa                                                                                                                               >  5:347419/1‑149 (MQ=255)
                            tCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCg                                                                                                                         >  6:11947/1‑149 (MQ=255)
                                gccgccGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCgg                                                                                                                        <  6:347419/146‑1 (MQ=255)
                                 ccgccgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGa                                                                                                                     <  4:171561/148‑1 (MQ=255)
                                  cgccgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTTGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGc                                                                                                                   <  5:11455/149‑1 (MQ=255)
                                  caccgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGc                                                                                                                   <  6:363193/147‑1 (MQ=255)
                                    ccgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCAt                                                                                                                 <  2:356593/149‑1 (MQ=255)
                                     cgACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATg                                                                                                                <  2:210102/149‑1 (MQ=255)
                                       aCGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTa                                                                                                              <  6:128428/149‑1 (MQ=255)
                                            gAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTacaaca                                                                                                         >  5:79312/1‑149 (MQ=255)
                                            gAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTacaa                                                                                                           >  2:155123/1‑147 (MQ=255)
                                            gAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTacaa                                                                                                           >  2:161096/1‑147 (MQ=255)
                                                 aCAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCATCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCTAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGGGAGCATGTACAACAGcccc                                                                                                    <  5:124508/149‑1 (MQ=255)
                                                 aCAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGccc                                                                                                     >  3:228736/1‑148 (MQ=255)
                                                 aCAAATCGGGCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGcccc                                                                                                    <  6:17389/149‑1 (MQ=255)
                                                     aTCGTTCTGCACCCGCGCCTTCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTTCTGCACACCCACCCGAGGCAGTGTATCGGGGACCTCTTCCTTTCTACAGCCGGTGAGCATTTACAACAGCCCCAGGc                                                                                                <  5:63108/149‑1 (MQ=255)
                                                     aTCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAgg                                                                                                 >  2:325200/1‑148 (MQ=255)
                                                      tCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCa                                                                                               <  6:428343/149‑1 (MQ=255)
                                                              cACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGTGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAAc                                                                                         <  1:415462/147‑1 (MQ=255)
                                                              cACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAAc                                                                                         <  2:376278/147‑1 (MQ=255)
                                                               aCCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACTGa                                                                                      >  1:19024/1‑149 (MQ=255)
                                                               aCCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGa                                                                                      >  2:326981/1‑149 (MQ=255)
                                                                 ccGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAAcc                                                                                        >  3:5470/1‑145 (MQ=255)
                                                                 ccGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAAcc                                                                                        <  4:5470/145‑1 (MQ=255)
                                                                 ccGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGCTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGa                                                                                      >  7:168124/1‑147 (MQ=255)
                                                                       cTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGAc                                                                              >  7:149971/1‑149 (MQ=255)
                                                                        tGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACg                                                                             >  6:162044/1‑149 (MQ=255)
                                                                         gCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACg                                                                             >  7:400387/1‑148 (MQ=255)
                                                                          cACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCa                                                                                               >  1:428565/1‑129 (MQ=255)
                                                                          cACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCa                                                                                               <  2:428565/129‑1 (MQ=255)
                                                                           aCGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCAt                                                                          <  1:189671/149‑1 (MQ=255)
                                                                           aCGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCAt                                                                          >  8:222042/1‑149 (MQ=255)
                                                                            cGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCAt                                                                          >  1:310400/1‑148 (MQ=255)
                                                                             gTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGCCCTCTTCCTTGCTACAGCAGGTGAGCATGTACAACAGCCCCAGGCAGACAACCCACAGCGGACGCATc                                                                         >  4:273665/1‑148 (MQ=255)
                                                                             gTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATc                                                                         >  5:53827/1‑148 (MQ=255)
                                                                             gTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTGGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGTTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCa                                                                        <  8:197290/149‑1 (MQ=255)
                                                                                gCCGTTCAAAGTGACCCTGGCAGCGAAATCTGTTGGTTGTACATGCTGCACACCCACCCGAGGCAGTGTCTCGTGTACCTCTTCCTTGCTACAGCCGTTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAAc                                                                      <  2:316355/148‑1 (MQ=255)
                                                                                gCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACg                                                                     <  5:11947/149‑1 (MQ=255)
                                                                                 ccGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGc                                                                    <  4:63246/149‑1 (MQ=255)
                                                                                     tCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATg                                                                >  5:412331/1‑149 (MQ=255)
                                                                                       aaaGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGAGGACGCATCAACGCCAGGCt                                                              >  8:369688/1‑149 (MQ=255)
                                                                                         aGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTTCAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTgg                                                            <  1:326981/149‑1 (MQ=255)
                                                                                         aGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTgg                                                            >  7:248893/1‑149 (MQ=255)
                                                                                         aGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTgg                                                            <  6:284403/149‑1 (MQ=255)
                                                                                         aGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTgg                                                            <  2:310400/149‑1 (MQ=255)
                                                                                           tGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGc                                                           <  8:400387/148‑1 (MQ=255)
                                                                                             aCCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTcc                                                        >  5:337830/1‑149 (MQ=255)
                                                                                               ccTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCctt                                                      >  3:179817/1‑149 (MQ=255)
                                                                                                 tGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCcttgc                                                    >  3:87688/1‑149 (MQ=255)
                                                                                                   gCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCcttgc                                                    <  1:161096/147‑1 (MQ=255)
                                                                                                   gCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCcttgc                                                    <  1:155123/147‑1 (MQ=255)
                                                                                                     aGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTtgt                                                <  6:53827/149‑1 (MQ=255)
                                                                                                     aGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTtgt                                                <  6:154688/149‑1 (MQ=255)
                                                                                                       cGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGc                                              <  5:162044/149‑1 (MQ=255)
                                                                                                              ggTGTGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAAt                                        <  6:417905/148‑1 (MQ=255)
                                                                                                              ggTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACTGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAAt                                        <  2:19024/148‑1 (MQ=255)
                                                                                                               gTGGGTTGTACCTGCTGCACACCCACGCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGa                                      >  1:11677/1‑149 (MQ=255)
                                                                                                                tGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGAt                                     <  8:5404/149‑1 (MQ=255)
                                                                                                                   gTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTtg                                  >  8:333836/1‑149 (MQ=255)
                                                                                                                    ttGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTtgt                                 >  3:191109/1‑149 (MQ=255)
                                                                                                                      gTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTtgtg                                <  6:412331/148‑1 (MQ=255)
                                                                                                                         ccTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGAt                            >  7:64642/1‑149 (MQ=255)
                                                                                                                            gctgcACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCa                                                                  <  6:398038/108‑1 (MQ=255)
                                                                                                                             ctgcACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGa                        >  1:178968/1‑149 (MQ=255)
                                                                                                                               gcACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACt                      >  7:28497/1‑149 (MQ=255)
                                                                                                                                cacaCCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACt                      <  4:191109/148‑1 (MQ=255)
                                                                                                                                 acacCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGAGCACAAATGATTTGTGGGATGCGACtgt                    <  4:87688/149‑1 (MQ=255)
                                                                                                                                  cacccaccCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACtgtg                   <  8:168124/149‑1 (MQ=255)
                                                                                                                                   acccacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACtgt                    >  5:200794/1‑147 (MQ=255)
                                                                                                                                   acccacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACtgt                    >  8:122423/1‑147 (MQ=255)
                                                                                                                                      cacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCg               >  1:67523/1‑149 (MQ=255)
                                                                                                                                      cacccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCg               >  4:131865/1‑149 (MQ=255)
                                                                                                                                       acccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACtgt                    >  6:93856/1‑143 (MQ=255)
                                                                                                                                       acccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACtgt                    <  5:93856/143‑1 (MQ=255)
                                                                                                                                        cccGAGACACTGCCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGCCAACCGCCAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGTTGCGACTGTGAGCg               >  5:271040/1‑147 (MQ=255)
                                                                                                                                        cccGAGACACTGCCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCg               >  3:136172/1‑147 (MQ=255)
                                                                                                                                        cccGAGACACTGCCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCg               >  5:245454/1‑147 (MQ=255)
                                                                                                                                        cccGAGACACTGCCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCATTGCTTGTGCACAAATGATTTGTGGGATGCGCCTGTGAGCg               >  5:265083/1‑147 (MQ=255)
                                                                                                                                         ccGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAg            <  8:149971/149‑1 (MQ=255)
                                                                                                                                               cAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAgg      <  2:178968/149‑1 (MQ=255)
                                                                                                                                                aGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGGGCGTAGTTCAggg     >  5:426484/1‑149 (MQ=255)
                                                                                                                                                aGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAggg     >  4:262323/1‑149 (MQ=255)
                                                                                                                                                    tctcGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTc  >  8:273263/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCGCCCACGTCGACGCTACGCGAAATGATCTTGCCGCCGACGCGGAATGACAAATCGGTCTGCACCCGCGCCTGCACGTCGCCGGTCAAAGTGACCCTGGCAGCGAAATCGGTGGGTTGTACCTGCTGCACACCCACCCGAGGCAGTGTCTCGGGGACCTCTTCCTTGCTACAGCCGGTGAGCATGTACAACAGCCCCAGGCAGACAACCGACAGCGGACGCATCAACGCCATGCTGGCTCCTTGCTTGTGCACAAATGATTTGTGGGATGCGACTGTGAGCGTAGTTCAGGGTTC  >  NC_002947/5902804‑5903099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑