Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,942,313	G→C	7.0%	R68G (CGG→GGG)	PP_5209 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,942,313	0	G	C	7.0%	296.7 / 6.8	96	R68G (CGG→GGG)	PP_5209	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (43/46);  new base C (3/4);  total (46/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

GCCTCGACGTTGCTCATGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCAAGATCCACGCTTTCACGAA  >  NC_002947/5942165‑5942460                                                                                                                                                       |                                                                                                                                                    gCCTCGACGTTGCTCATGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCcg                                                                                                                                                     >  1:37958/1‑149 (MQ=255)  ccTCGACGTTGCTCATGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCcgc                                                                                                                                                    >  6:154075/1‑149 (MQ=255)      gACGTTGCTCATGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCgg                                                                                                                                                  <  6:382532/147‑1 (MQ=255)          ttGCTCATGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGcc                                                                                                                                            <  2:319426/149‑1 (MQ=255)            gCTCATGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCCGCGGCTGcgc                                                                                                                                           <  8:304691/148‑3 (MQ=255)            gCTCATGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCCGCGGCTGcgc                                                                                                                                           <  7:217354/148‑3 (MQ=255)             cTCATGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGAGCCGCGGTTCGGGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGGCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGt                                                                                                                                          <  7:318369/148‑1 (MQ=255)              tCATGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCACTTTTGAGGGGCCACGTACGCATTTAACACGCGCACCCCCGGGCTGCCGt                                                                                                                                          >  1:147322/1‑149 (MQ=255)              tCATGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCCGCCGCGGGCTGCCGTc                                                                                                                                         >  6:67913/1‑148 (MQ=255)              tCATGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTc                                                                                                                                         >  8:291484/1‑148 (MQ=255)               cATGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGa                                                                                                                                       <  4:4138/149‑1 (MQ=255)                aTGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCCGCGGCTGcgcg                                                                                                                                          >  3:297935/1‑142 (MQ=255)                aTGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCCGCGGCTGcgcg                                                                                                                                          <  4:297935/145‑4 (MQ=255)                 tGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAgc                                                                                                                                     <  4:377593/149‑1 (MQ=255)                 tGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAgc                                                                                                                                     <  4:379631/149‑1 (MQ=255)                 tGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAgc                                                                                                                                     <  6:356735/149‑1 (MQ=255)                  gCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTcgcgcg                                                                                                                                    >  4:346145/1‑149 (MQ=255)                    tGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCCC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCa                                                                                                                                  >  4:416708/1‑149 (MQ=255)                     gTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTAGTACACGCGCAGCCGCTGGCTGACGTCGAGCGCa                                                                                                                                  >  4:315006/1‑148 (MQ=255)                      tCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCa                                                                                                                                  >  6:372219/1‑147 (MQ=255)                      tCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACACGCAGCCGCGGGCTGCCGTCGAGCGCATa                                                                                                                                <  6:256937/149‑1 (MQ=255)                          ccGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTg                                                                                                                            <  2:68340/149‑1 (MQ=255)                            gTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGcc                                                                                                                          <  3:217522/149‑1 (MQ=255)                                  tgGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACg                                                                                                                      >  1:414901/1‑147 (MQ=255)                                    ggTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCagccatag                                                                                                                  >  1:372495/1‑142 (MQ=255)                                    ggTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGaa                                                                                                                    <  3:50032/147‑1 (MQ=255)                                    ggTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAgg                                                                                                                  <  5:154075/149‑1 (MQ=255)                                    ggTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAgg                                                                                                                  <  7:291513/149‑1 (MQ=255)                                      tGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAgggc                                                                                                                >  7:225581/1‑149 (MQ=255)                                      tGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCCGCGGCTGcgcg                                                                                                                                          >  1:116576/1‑120 (MQ=255)                                      tGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCCGCGGCTGcgcg                                                                                                                                          <  2:116576/123‑4 (MQ=255)                                           aGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCt                                                                                                           >  5:426853/1‑149 (MQ=255)                                            gTGCCACCAGCTGGTTACGGATCAGCGTTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCATCGAAGGGCGGGCTc                                                                                                          <  7:224244/149‑1 (MQ=255)                                             tGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAAGGAAggggtgccc                                                                                                           >  5:224720/1‑141 (MQ=255)                                             tGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCt                                                                                                           >  3:62955/1‑147 (MQ=255)                                               ccaccaGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGa                                                                                                       >  2:273576/1‑149 (MQ=255)                                                caccaGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGAc                                                                                                      <  8:225581/149‑1 (MQ=255)                                                 accaGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACTAAGGGCGGGCTCAGACt                                                                                                     <  2:414901/149‑1 (MQ=255)                                                    aGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGAt                                                                                                  <  6:324475/149‑1 (MQ=255)                                                      cTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCATGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCCGGCTCAGACTGATg                                                                                                 >  3:332481/1‑148 (MQ=255)                                                       tGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGAGGGCTCAGACTGATg                                                                                                 >  5:256406/1‑147 (MQ=255)                                                         gTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTa                                                                                               <  5:333641/147‑1 (MQ=255)                                                              ggATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGAcc                                                                                        >  8:299639/1‑149 (MQ=255)                                                               gATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACt                                                                                                     <  5:63816/135‑1 (MQ=255)                                                               gATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACt                                                                                                     >  6:63816/1‑135 (MQ=255)                                                                      ggTTCGTTGGGCTTTACGGTCTTGGCCGCTTGGTCGCCCGTCAC‑‑TCGCAGGGCCACGTCAGCGTTGTAGACGCGCAGCCGCGGGCTGCCGTCTAGCGCATAGTTGCCAACGATGGGCGGGCTCAGACTGATGTATGCGACCTTGTGCAc                                                                                <  8:346099/149‑1 (MQ=255)                                                                       gTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCAcc                                                                               >  1:215622/1‑149 (MQ=255)                                                                          cGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCcct                                                                            >  4:428234/1‑149 (MQ=255)                                                                           gtggtgCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGACGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCcctt                                                                           >  5:309901/1‑149 (MQ=255)                                                                             ggtgCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCccttcc                                                                         >  4:202506/1‑149 (MQ=255)                                                                              gtgCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAGCGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCccttcct                                                                        >  6:100904/1‑149 (MQ=255)                                                                                gCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCAcccc                                                                             <  5:129658/142‑1 (MQ=255)                                                                                gCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCAcccc                                                                             >  6:129658/1‑142 (MQ=255)                                                                                 cTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTg                                                                      <  1:273576/148‑1 (MQ=255)                                                                                  tttACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGc                                                                    >  1:99368/1‑149 (MQ=255)                                                                                   ttACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCGACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCt                                                                   >  7:377511/1‑149 (MQ=255)                                                                                    tACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGc                                                                    <  5:372219/147‑1 (MQ=255)                                                                                     aCCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCtt                                                                  <  6:135685/148‑1 (MQ=255)                                                                                     aCCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTc                                                                 >  1:365676/1‑149 (MQ=255)                                                                                        gTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTc                                                              >  7:869/1‑149 (MQ=255)                                                                                             ggCCGCTTCGTCGCCTGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCa                                                         >  3:80634/1‑149 (MQ=255)                                                                                             ggCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCGCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGc                                                           <  4:156517/147‑1 (MQ=255)                                                                                                cGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATgg                                                      <  8:377511/149‑1 (MQ=255)                                                                                                 gCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGc                                                     >  7:305652/1‑149 (MQ=255)                                                                                                  cTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGcc                                                    <  7:299639/149‑1 (MQ=255)                                                                                                        cgCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGcc                                              >  8:293587/1‑149 (MQ=255)                                                                                                         gCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCCCCCCTTCCTTGGCTTCCTCGGCCCTGGccgccgccg                                             >  3:397166/1‑149 (MQ=255)                                                                                                          cccGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCgg                                            >  1:267627/1‑149 (MQ=255)                                                                                                          cccGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCgg                                            <  2:365676/149‑1 (MQ=255)                                                                                                          cccGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCATTGGCTTCCTCGGCCATGGCCGCAGCCgg                                            <  7:291484/149‑1 (MQ=255)                                                                                                            cGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCgg                                            >  1:379204/1‑147 (MQ=255)                                                                                                            cGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGgca                                          <  6:426853/149‑1 (MQ=255)                                                                                                             gTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGgcag                                         <  3:315006/149‑1 (MQ=255)                                                                                                             gTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGgcag                                         <  7:280521/149‑1 (MQ=255)                                                                                                                      gcAGGGCCACGTCATCCGTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCagcagcagc                                  <  6:68893/149‑1 (MQ=255)                                                                                                                      gcAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCagcagcagc                                  <  2:379204/149‑1 (MQ=255)                                                                                                                       cAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAgcg                                 <  3:202506/149‑1 (MQ=255)                                                                                                                        aGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAgcgc                                >  4:203479/1‑149 (MQ=255)                                                                                                                         gggCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGcc                               <  4:80634/149‑1 (MQ=255)                                                                                                                         gggCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGcc                               <  3:416708/149‑1 (MQ=255)                                                                                                                          ggCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAgcgc                                >  6:158490/1‑147 (MQ=255)                                                                                                                                 tCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCAt                       >  4:341776/1‑149 (MQ=255)                                                                                                                                   aGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATc                      <  3:341776/148‑1 (MQ=255)                                                                                                                                   aGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATc                      <  3:203479/148‑1 (MQ=255)                                                                                                                                    gCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCaa                    <  2:147322/149‑1 (MQ=255)                                                                                                                                     ccTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATc                      >  5:294817/1‑146 (MQ=255)                                                                                                                                     ccTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCAAg                   <  7:293587/149‑1 (MQ=255)                                                                                                                                     ccTTGTACACGCGCAGCCGCGGGCTACCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCAAg                   <  4:62955/149‑1 (MQ=255)                                                                                                                                             acGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCATCACCATCAAGATCCACGc           >  8:319649/1‑149 (MQ=255)                                                                                                                                             acGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCAAGATCCACGc           >  1:426006/1‑149 (MQ=255)                                                                                                                                               gcgcAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATc                      <  5:147122/136‑1 (MQ=255)                                                                                                                                               gcgcAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATc                      >  6:147122/1‑136 (MQ=255)                                                                                                                                               gcgcAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCAAGATCCACGCtt         >  3:88753/1‑149 (MQ=255)                                                                                                                                               gcgcAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCAAGATCCACGCtt         >  3:110753/1‑149 (MQ=255)                                                                                                                                               gcgcAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCAAGATCCACGCtt         >  2:95923/1‑149 (MQ=255)                                                                                                                                                cgcAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCAAGATCCACGCttt        <  2:426006/149‑1 (MQ=255)                                                                                                                                                 gcAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCAAGATCCACGCTTTc       <  7:319649/149‑1 (MQ=255)                                                                                                                                                   aGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCAAGATCCACGCTTTCAc     <  7:3020/149‑1 (MQ=255)                                                                                                                                                      cgcgTGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCAAGATCCACGCTTTCACGaa  <  8:305652/149‑1 (MQ=255)                                                                                                                                                      cgcgGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCAAGATCCACGCTTTCACGaa  <  6:256406/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                    GCCTCGACGTTGCTCATGCTGTCCACCGTCTGCTGGGTGAACAGTGCCACCAGCTGGTTACGGATCAGCGGTTCGTGGTGCTTTACCGTCTTGGCCGCTTCGTCGCCCGTCAC‑‑GCGCAGGGCCACGTCAGCCTTGTACACGCGCAGCCGCGGGCTGCCGTCGAGCGCATAGTTGCCAACGAAGGGCGGGCTCAGACTGATGTAGGCGACCTTGGGCACCCCTTCCTTGGCTTCCTCGGCCATGGCCGCAGCCGGCAGCAGCAGCGCCAGCACCATCAAGATCCACGCTTTCACGAA  >  NC_002947/5942165‑5942460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑