Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,959,996	A→T	8.9%	noncoding (73/103 nt)	PP_mr63 ←	rnk_pseudo ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,959,996	0	A	T	8.9%	259.6 / 12.8	98	noncoding (73/103 nt)	PP_mr63	rnk_pseudo ncRNA
Reads supporting (aligned to +/- strand):  ref base A (45/44);  new base T (4/5);  total (49/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

GCGTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTC  >  NC_002947/5959848‑5960141                                                                                                                                                      |                                                                                                                                                  gCGTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCaa                                                                                                                                                   <  2:394627/149‑1 (MQ=255)      ctctCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGc                                                                                                                                               <  2:135842/148‑1 (MQ=255)       tctcGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCgg                                                                                                                                             >  4:418955/1‑149 (MQ=255)           gtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCggg                                                                                                                                            <  7:10704/146‑1 (MQ=255)           gtcgAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGtt                                                                                                                                         <  4:35575/149‑1 (MQ=255)              gAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTga                                                                                                                                      <  3:80594/149‑1 (MQ=255)               aGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCTTGGGCGGGGttttgc                                                                                                                                     <  8:375312/149‑4 (MQ=255)               aGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCTTGGGCGGGGttttgc                                                                                                                                     <  3:139433/149‑4 (MQ=255)                ggCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCTTGGGCGGGGttttgct                                                                                                                                    <  7:29512/149‑5 (MQ=255)                 gCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGATGATTAGTGAAGGCTTGGTGCTCATGATCCAGTCTGACTCCTTTGAAAAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAAGCCCGCCCAAGGGCGGGGTTTGAGaa                                                                                                                                   <  1:134114/149‑1 (MQ=255)                 gCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGaa                                                                                                                                   <  6:64810/149‑1 (MQ=255)                 gCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGaa                                                                                                                                   <  6:71283/149‑1 (MQ=255)                 gCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGACGGGGTTTGAGaa                                                                                                                                   <  5:146597/149‑1 (MQ=255)                   tGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCggg                                                                                                                                            <  4:402610/138‑1 (MQ=255)                   tGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCggg                                                                                                                                            <  4:409100/138‑1 (MQ=255)                   tGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCggg                                                                                                                                            >  3:409100/1‑138 (MQ=255)                   tGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCggg                                                                                                                                            >  3:402610/1‑138 (MQ=255)                   tGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAgg                                                                                                                                 >  6:192308/1‑149 (MQ=255)                   tGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAgg                                                                                                                                 >  2:278172/1‑149 (MQ=255)                    gTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGc                                                                                                                                <  5:233449/149‑1 (MQ=255)                     tCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGc                                                                                                                                <  5:184526/148‑1 (MQ=255)                        aTCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTg                                                                                                                            <  6:412376/149‑1 (MQ=255)                             aCGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTc                                                                                                                       <  5:206454/149‑1 (MQ=255)                               gCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCAc                                                                                                                     <  4:139930/149‑1 (MQ=255)                               gCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCAc                                                                                                                     >  3:165186/1‑149 (MQ=255)                                        gCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAg                                                                                                             >  4:172155/1‑148 (MQ=255)                                           gTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAAcc                                                                                                         >  6:389534/1‑149 (MQ=255)                                            tACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAAc                                                                                                          <  3:365004/147‑1 (MQ=255)                                              cGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGa                                                                                                      <  6:410497/149‑1 (MQ=255)                                               gTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGac                                                                                                     >  2:325802/1‑149 (MQ=255)                                               gTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGac                                                                                                     <  6:192531/149‑1 (MQ=255)                                               gTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGa                                                                                                      >  2:320077/1‑148 (MQ=255)                                                   aaTCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTa                                                                                                 >  3:158495/1‑149 (MQ=255)                                                    aTCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTAc                                                                                                <  1:171932/149‑1 (MQ=255)                                                    aTCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGACAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTAc                                                                                                <  5:371217/149‑1 (MQ=255)                                                       gggTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACcac                                                                                             <  1:324327/149‑1 (MQ=255)                                                         gTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAg                                                                                           >  6:37836/1‑149 (MQ=255)                                                            aGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCg                                                                                        <  3:172155/149‑1 (MQ=255)                                                                  aGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCgg                                                                                       <  7:321834/144‑1 (MQ=255)                                                                  aGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCgg                                                                                       >  8:321834/1‑144 (MQ=255)                                                                  aGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCaaa                                                                                  <  5:43657/149‑1 (MQ=255)                                                                    ggAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAAt                                                                                 >  3:196111/1‑148 (MQ=255)                                                                    ggAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGCCAAAt                                                                                 >  1:104336/1‑148 (MQ=255)                                                                    ggAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACAACAGCCGGGCAAAt                                                                                 >  5:126943/1‑148 (MQ=255)                                                                      aaGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATAc                                                                               >  5:434595/1‑148 (MQ=255)                                                                       aGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTc                                                                             <  5:93177/149‑1 (MQ=255)                                                                           ttGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGc                                                                         <  4:165186/149‑1 (MQ=255)                                                                               tGCTCATGATCCAGTCAGACTCCTTTTAACAGGCATTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCt                                                                     <  2:353752/149‑1 (MQ=255)                                                                               tGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCt                                                                     <  4:196111/149‑1 (MQ=255)                                                                                gCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGGGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTg                                                                    >  1:300679/1‑149 (MQ=255)                                                                                  tCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGACCCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTgcg                                                                  >  6:422139/1‑149 (MQ=255)                                                                                   cATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACACGACACTACCACAGCCGGGCAAATACTCAAGCCCCTgcgc                                                                 >  6:88043/1‑149 (MQ=255)                                                                                    aTGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCtt                                                                                                                              >  7:396778/1‑87 (MQ=255)                                                                                    aTGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCtt                                                                                                                              <  8:396778/87‑1 (MQ=255)                                                                                    aTGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCt                                                                >  1:322365/1‑149 (MQ=255)                                                                                    aTGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCt                                                                <  5:422139/149‑1 (MQ=255)                                                                                     tGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGgcac                                                                                                   >  8:66380/1‑113 (MQ=255)                                                                                      gATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGAGGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGAGCt                                                                >  2:151371/1‑147 (MQ=255)                                                                                      gATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGacac                                                                                                   <  3:117609/112‑1 (MQ=255)                                                                                      gATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGacac                                                                                                   >  4:117609/1‑112 (MQ=255)                                                                                       aTCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCa                                                              <  2:322365/148‑1 (MQ=255)                                                                                       aTCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCa                                                              <  3:418955/148‑1 (MQ=255)                                                                                        tCCAGTCACACGCCTCTGACCACGCAGTTTCCAACGTCAA‑AATAAACCACAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTGTGGCGGCACCGCCGCAGAACCCGACACTACCACACCCGGGCAAATACTCAAGCCCCTGCGCTCAgt                                                            <  8:305541/149‑1 (MQ=255)                                                                                          taGTCTGTCTGCTTTGAAGAGGGATTTTTAAATGTTCA‑AATAAAGCAAAACGCCGCCGTTGGGGGGTGTTTTAGATGGCTTTGGCGTCACGTACTCATAACCTGACAATACCACAGCGGGGAAAATACTCAAGCCCCTGCGCTCAgtg                                                           <  6:86577/147‑1 (MQ=255)                                                                                          cAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAgtgt                                                          <  1:320077/149‑1 (MQ=255)                                                                                          cAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAgtgt                                                          <  1:325802/149‑1 (MQ=255)                                                                                           aGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCa                                                              <  1:102976/144‑1 (MQ=255)                                                                                           aGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCa                                                              >  2:102976/1‑144 (MQ=255)                                                                                            gTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCAGAATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGAGCTCAGtgtg                                                         >  1:291264/1‑149 (MQ=255)                                                                                                gACTCCTTTGAACAGGCAGTTTTCAAAGTCCA‑AAAAAACCAAAACCCCGCCCAAGGGCGGGGTTAGAGAAGGGTTAGGCTTCACCGACGAAGAACCCGACACTACCACAGACGGGCAAATACTCAAGCCCCTGAGCTCATTGTCCCt                                                      >  7:139220/1‑147 (MQ=255)                                                                                                  cTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGc                                                  >  2:244310/1‑149 (MQ=255)                                                                                                  cTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAACGGCGGGCTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGc                                                  >  3:188376/1‑149 (MQ=255)                                                                                                   tCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGcc                                                 >  7:346338/1‑149 (MQ=255)                                                                                                     cTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCt                                                <  2:291264/148‑1 (MQ=255)                                                                                                      tttGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGc                                              >  7:5066/1‑149 (MQ=255)                                                                                                             aGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAGCCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCgcgc                                        <  2:222990/148‑1 (MQ=255)                                                                                                             aGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAGCCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCgcgc                                        >  1:222990/1‑148 (MQ=255)                                                                                                             aGGCAGTTTTCAATGTCCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGCCTTTGGCGTCACATACGCAGAACACGACAATACCACACACGCGCAAATAATCAAGCCACAGCGCACAGTGTGCCTTAGCCTGCTCgcgc                                        >  3:242980/1‑148 (MQ=255)                                                                                                              ggCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCTCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCaa                                      <  1:278172/149‑1 (MQ=255)                                                                                                                 aGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGAc                                   >  5:231327/1‑149 (MQ=255)                                                                                                                            tgCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcgc                        >  2:132733/1‑149 (MQ=255)                                                                                                                            tgCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTcgc                        >  7:182143/1‑149 (MQ=255)                                                                                                                               a‑aaTAAAGAAAAACCCAGCCGATGTGGGGTGTTTGAGAAGGCTTTGGCGTCACCGACGAAGAACCCGACACTACCACAGTCTTGCAAATACTCAAGCCGCTGCGCTCAGTGTGCCTTAGCGTGCTCTCGCAAGACCTGGGCCTCGCgg                      <  4:158495/148‑1 (MQ=255)                                                                                                                                    cttctcaaaCCCCGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCgg                      >  4:353407/7‑144 (MQ=255)                                                                                                                                      aGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCt               >  7:64549/1‑149 (MQ=255)                                                                                                                                       gCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTg              >  7:124746/1‑149 (MQ=255)                                                                                                                                          aaaCCCCGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCg           >  7:346990/1‑149 (MQ=255)                                                                                                                                          aaaCCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCg           >  8:232243/1‑149 (MQ=255)                                                                                                                                           aaCCCCGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCgg          >  8:136504/1‑149 (MQ=255)                                                                                                                                           aaCCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGc                                                  <  6:345122/109‑1 (MQ=255)                                                                                                                                           aaCCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGc                                                  >  5:345122/1‑109 (MQ=255)                                                                                                                                             ccccGCCCTTGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCAGgcg        >  3:76017/1‑149 (MQ=255)                                                                                                                                                 gCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGt    <  6:434595/149‑1 (MQ=255)                                                                                                                                                  cccAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGtt   >  7:13222/1‑149 (MQ=255)                                                                                                                                                   ccAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCg           <  6:383740/140‑1 (MQ=255)                                                                                                                                                   ccAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCg           >  5:383740/1‑140 (MQ=255)                                                                                                                                                   ccAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTc  >  2:107104/1‑149 (MQ=255)                                                                                                                                                   ccAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTc  >  6:265375/1‑149 (MQ=255)                                                                                                                                                   ccAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTc  <  5:389534/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                  GCGTACTCTCGTCGAGGCTGTCGATCAGACGCTCCAGACGCTGTACGTCCAATCGGGTGAGGATGAGGGAAGGCTTGGTGCTCATGATCCAGTCAGACTCCTTTGAACAGGCAGTTTTCAATGTGCA‑AATAAAGCAAAACCCCGCCCAAGGGCGGGGTTTGAGAAGGCTTTGGCGTCACCGACGCAGAACCCGACACTACCACAGCCGGGCAAATACTCAAGCCCCTGCGCTCAGTGTGCCTTAGCCTGCTCGCGCAAGACCTGGGCCTCGCGGCAGATCTGCCGGCGCCGTTC  >  NC_002947/5959848‑5960141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑