Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,963,851 C→A 11.6% I217I (ATC→ATA)  PP_5229 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,963,8510CA11.6% 221.6 / 13.0 82I217I (ATC→ATA) PP_5229hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (30/42);  new base A (6/4);  total (36/46)
Fisher's exact test for biased strand distribution p-value = 3.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

CCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTCGCGACCT  >  NC_002947/5963704‑5963999
                                                                                                                                                   |                                                                                                                                                    
ccTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATc                                                                                                                                                      <  5:52491/148‑1 (MQ=255)
 cTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGc                                                                                                                                                    >  3:392688/1‑149 (MQ=255)
 cTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGc                                                                                                                                                    >  6:135879/1‑149 (MQ=255)
    ttCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGa                                                                                                                                                 <  5:191040/149‑1 (MQ=255)
       ggTGAAGAACAGCGCCGGGAAGTAGTCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACACGCAACACTACAAGAGCAGAGTCGGTACCCTTTTCCTCGGCTAGATAGCCGAGGt                                                                                                                                              >  8:343579/1‑149 (MQ=255)
       ggTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCGATATAGCCGAgg                                                                                                                                               <  4:281246/148‑1 (MQ=255)
       ggTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTATTCCTCGGCGATATAGCCGAgga                                                                                                                                              <  6:442341/149‑2 (MQ=255)
        gTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCGATATAGCCGAggaa                                                                                                                                             <  6:323396/149‑3 (MQ=255)
          gaagaaCAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGCCCCGCAACACTACAAGAGCAGCGTCGGGACCCTTTTCCTCGGCGATATCGCCGAGGTGcg                                                                                                                                           >  7:296106/1‑148 (MQ=255)
              aaCAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGc                                                                                                                                       <  7:258488/149‑1 (MQ=255)
                   cgcCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGt                                                                                                                                  <  2:122497/149‑1 (MQ=255)
                    gcCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGtt                                                                                                                                 <  6:420232/149‑1 (MQ=255)
                    gcCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGtt                                                                                                                                 >  5:291358/1‑149 (MQ=255)
                      cGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGGCGCGAGCGGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTGGGCTATATCGCCGAGGTGCTTGGCCGCGTtgt                                                                                                                               <  2:377863/149‑1 (MQ=255)
                      cGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTtgt                                                                                                                               <  2:424597/149‑1 (MQ=255)
                      cGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCAATATCGCCGAGGTGCTTGGCCGCGTtgt                                                                                                                               <  1:246468/149‑1 (MQ=255)
                        ggAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCcgcg                                                                                                                                   >  7:275481/1‑143 (MQ=255)
                        ggAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCcgcg                                                                                                                                   <  8:275481/143‑1 (MQ=255)
                           aGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCAc                                                                                                                          <  1:206221/149‑1 (MQ=255)
                              agtcTCCAGCGCCGTCGCTGCTGTTGCGTACCAGGGTCTGCATGGGGTTCTGGCCATCGGCCGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCAcgc                                                                                                                        <  8:296106/145‑1 (MQ=255)
                                  tCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTAAc                                                                                                                   >  6:41270/1‑149 (MQ=255)
                                     aGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCAcc                                                                                                                  >  3:399692/1‑147 (MQ=255)
                                      gcgcCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGACGCGACCCGCTACACTACAAGAGCAGCGTCGGTACCCTTTTCATCGGGTTTATCGACGAGGTGCTTGGCCGCGTTGTGCCACGCCTCAc                                                                                                                   >  6:155782/1‑145 (MQ=255)
                                      gcgcCGTCGCAGCTCTTGAGTACCAGGCGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCCCAACACTACAAGAGCAGCGTCGGTACCCTTATCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCAc                                                                                                                   <  5:155782/145‑1 (MQ=255)
                                       cgcCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCTTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGa                                                                                                              <  1:120758/149‑1 (MQ=255)
                                           gTGGCAGCTCTTTAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCt                                                                                                          <  7:17114/149‑1 (MQ=255)
                                           gTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCt                                                                                                          <  2:62294/149‑1 (MQ=255)
                                           gTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCACAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCt                                                                                                          <  6:149207/149‑1 (MQ=255)
                                              gctgCTCTTGAGTACCAGGGGCTGCAAGGGGTTCTGGCCATCGGCAGACGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTgcg                                                                                                       >  6:35624/4‑149 (MQ=255)
                                                aGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGcc                                                                                                     <  2:311544/149‑1 (MQ=255)
                                                  ctctTGAGTACCAGGGGCTGCATGGGGTTCTGGACATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCg                                                                                                   <  8:391217/149‑1 (MQ=255)
                                                     ttGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCgg                                                                                                  <  2:406686/147‑1 (MQ=255)
                                                       gAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGt                                                                                                 <  7:343579/146‑1 (MQ=255)
                                                       gAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTa                                                                                                <  6:291358/147‑1 (MQ=255)
                                                       gAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGACACGCGTCACCCAGACCCTGCGCCCGGTAc                                                                                               <  5:135879/148‑1 (MQ=255)
                                                         gTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCt                                                                                            >  3:18944/1‑149 (MQ=255)
                                                         gTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCt                                                                                            >  1:287291/1‑149 (MQ=255)
                                                            ccAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCtgat                                                                                         <  1:241580/149‑1 (MQ=255)
                                                            ccAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCtgat                                                                                         <  5:306388/149‑1 (MQ=255)
                                                            ccAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTAACCCAGACCCTGCGCCCGGTACGCtgat                                                                                         <  5:41270/149‑1 (MQ=255)
                                                               ggggCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTa                                                                                                >  4:356238/1‑139 (MQ=255)
                                                               ggggCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTa                                                                                                <  3:356238/139‑1 (MQ=255)
                                                                   cTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGc                                                                                  <  6:89848/149‑1 (MQ=255)
                                                                          gggTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTg                                                                            <  2:287291/148‑1 (MQ=255)
                                                                          gggTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTg                                                                            >  5:65019/1‑148 (MQ=255)
                                                                           ggTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGa                                                                          <  8:19474/149‑1 (MQ=255)
                                                                                     aTCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTgc                                                                >  8:32296/1‑149 (MQ=255)
                                                                                       cGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTgcg                                                               >  8:46000/1‑148 (MQ=255)
                                                                                         gCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGcac                                                            <  3:390358/149‑1 (MQ=255)
                                                                                               gcgACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCacac                                                          <  3:357073/145‑1 (MQ=255)
                                                                                               gcgACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCacac                                                          >  4:357073/1‑145 (MQ=255)
                                                                                                     cGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGCCCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAAc                                                 >  6:432493/1‑148 (MQ=255)
                                                                                                      gCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGc                                                                                             <  6:76633/103‑1 (MQ=255)
                                                                                                      gCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTgcgc                                                              <  3:354413/134‑1 (MQ=255)
                                                                                                      gCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTgcgc                                                              >  4:354413/1‑134 (MQ=255)
                                                                                                      gCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAAc                                                 >  5:422570/1‑147 (MQ=255)
                                                                                                      gCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAAc                                                 >  2:226467/1‑147 (MQ=255)
                                                                                                            cTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTCCGCACACCTGCGCAACGAGCGCCa                                         <  4:392688/149‑1 (MQ=255)
                                                                                                               cAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGgt                                      >  7:307058/1‑149 (MQ=255)
                                                                                                               cAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGGTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAg                                        <  8:359529/147‑1 (MQ=255)
                                                                                                                aaGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGgtg                                     <  5:321683/149‑1 (MQ=255)
                                                                                                                 agagCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGgtgt                                    >  7:38831/1‑149 (MQ=255)
                                                                                                                     cagcGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGa                                <  8:307058/149‑1 (MQ=255)
                                                                                                                      agcGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAg                               <  4:399692/149‑1 (MQ=255)
                                                                                                                      agcGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAg                               <  5:111189/149‑1 (MQ=255)
                                                                                                                        cGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGca                             >  6:124903/1‑149 (MQ=255)
                                                                                                                            ggTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACTCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCacac                          >  4:261063/1‑148 (MQ=255)
                                                                                                                               aCCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCg                                  >  4:28478/1‑137 (MQ=255)
                                                                                                                               aCCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCg                                  <  3:28478/137‑1 (MQ=255)
                                                                                                                                       ccTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAAc                                                 <  8:147987/114‑1 (MQ=255)
                                                                                                                                       ccTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCt               <  1:226467/148‑1 (MQ=255)
                                                                                                                                       ccTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAg                               >  6:183976/1‑132 (MQ=255)
                                                                                                                                       ccTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAg                               <  5:183976/132‑1 (MQ=255)
                                                                                                                                       ccTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTa              >  1:336048/1‑149 (MQ=255)
                                                                                                                                        cTCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTAc             >  5:112448/1‑149 (MQ=255)
                                                                                                                                         tCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAggatcatc            >  1:186441/1‑143 (MQ=255)
                                                                                                                                         tCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTAcc            >  4:414201/1‑149 (MQ=255)
                                                                                                                                         tCGGCTATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAAAGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTAcc            >  3:64396/1‑149 (MQ=255)
                                                                                                                                         tCGGCGATATAGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTAcc            >  6:51379/1‑149 (MQ=255)
                                                                                                                                            gCTAAATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTc         >  8:8845/1‑149 (MQ=255)
                                                                                                                                             cTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTcg        <  5:35624/149‑1 (MQ=255)
                                                                                                                                             cTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAAACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTcg        >  1:89419/1‑149 (MQ=255)
                                                                                                                                                 atCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTCGCGAc    >  6:370226/1‑149 (MQ=255)
                                                                                                                                                   cGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTCGCGACCt  >  8:178826/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CCTGTTCGGTGAAGAACAGCGCCGGGAAGTAGGCTCCAGCGCCGTGGCAGCTCTTGAGTACCAGGGGCTGCATGGGGTTCTGGCCATCGGCAGCCGCGACCCGCAACACTACAAGAGCAGCGTCGGTACCCTTTTCCTCGGCTATATCGCCGAGGTGCTTGGCCGCGTTGTGCCACGCGTCACCCAGACCCTGCGCCCGGTACGCTGATGAAACGCCAGCTGGAGGCTTATTGCGCACACCTGCGCAACGAGCGCCAGGTGTCGGAGCACACCTCGCAGGGCTACCGTCGCGACCT  >  NC_002947/5963704‑5963999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑