Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,965,573 C→T 8.8% intergenic (+71/+29) had → / ← PP_5232 (S)‑2‑haloacid dehalogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,965,5730CT 8.8% 252.0 / 9.8 90intergenic (+71/+29)had/PP_5232(S)‑2‑haloacid dehalogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (38/44);  new base T (2/6);  total (40/50)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

GACTGGCGATCAGGCGCCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAATTCTTCCATCGCCTTGGC  >  NC_002947/5965427‑5965718
                                                                                                                                                  |                                                                                                                                                  
gACTGGCGATCAGGCGCCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGc                                                                                                                                                    <  6:223998/147‑1 (MQ=255)
   tGGCGATCAGGCGCCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGAcgg                                                                                                                                               >  2:101176/1‑149 (MQ=255)
   tGGCGATCAGGCGCCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGACGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGAcgg                                                                                                                                               >  2:182382/1‑149 (MQ=255)
      cGATCAGGCGCCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGAcggcgg                                                                                                                                            <  3:36618/149‑1 (MQ=255)
       gATCAGGCGCCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCggg                                                                                                                                           >  7:62880/1‑149 (MQ=255)
       gATCAGGCGCCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCggg                                                                                                                                           <  4:245239/149‑1 (MQ=255)
         tCAGGCGCCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑t                                                                                                                                        >  2:267/1‑149 (MQ=255)
           aGGCGCCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑tt                                                                                                                                       <  2:275032/148‑1 (MQ=255)
            ggCGCCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑tttt                                                                                                                                     <  5:435889/149‑1 (MQ=255)
             gcgcCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCAAAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑ttttt                                                                                                                                    >  7:72921/1‑149 (MQ=255)
              cgcCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCTTGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCCGTCGCTGCGGGC‑tttttt                                                                                                                                   <  5:209065/149‑1 (MQ=255)
              cgcCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCCGTCGCTGCGGGC‑tttttt                                                                                                                                   <  3:320323/149‑1 (MQ=255)
              cgcCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCCGTCGCTGCGGGC‑tttttt                                                                                                                                   <  5:349049/149‑1 (MQ=255)
               gcCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCCGTCGCTGCGGGC‑tttttta                                                                                                                                  <  6:148430/149‑2 (MQ=255)
               gcCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCCGTCGCTGCGGGC‑tttttta                                                                                                                                  <  8:135097/149‑2 (MQ=255)
               gcCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTg                                                                                                                                  >  4:69680/1‑149 (MQ=255)
                ccGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGc                                                                                                                                 <  3:69680/149‑1 (MQ=255)
                  ggATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGTCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGa                                                                                                                               <  7:333806/149‑1 (MQ=255)
                  ggATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGa                                                                                                                               <  7:339766/149‑1 (MQ=255)
                   gATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGAt                                                                                                                              <  6:276697/149‑1 (MQ=255)
                        cGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGc                                                                                                                          >  1:285745/1‑148 (MQ=255)
                         gAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCa                                                                                                                        >  2:194040/1‑149 (MQ=255)
                              ccAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTg                                                                                                                                  >  7:372238/1‑134 (MQ=255)
                              ccAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTg                                                                                                                                  <  8:372238/134‑1 (MQ=255)
                                aGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑ttttt                                                                                                                                    >  6:114322/1‑130 (MQ=255)
                                aGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑ttttt                                                                                                                                    <  5:114322/130‑1 (MQ=255)
                                      tGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCCCAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGggcg                                                                                                           >  4:382995/1‑149 (MQ=255)
                                      tGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGggcg                                                                                                           >  7:263757/1‑149 (MQ=255)
                                        tCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGggcg                                                                                                           <  6:401943/147‑1 (MQ=255)
                                          gCAGCTGCCCGACGTGCTCGCGCGCTGTCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTg                                                                                                       <  1:182382/149‑1 (MQ=255)
                                           cAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGc                                                                                                      >  5:76664/1‑149 (MQ=255)
                                                 cccGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTAc                                                                                                 <  6:37330/148‑1 (MQ=255)
                                                 cccGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACt                                                                                                >  6:226209/1‑149 (MQ=255)
                                                     aCGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACttgtt                                                                                            >  1:121830/1‑149 (MQ=255)
                                                          cTCGCGCGCTGGCGGGTAGGGTCGCGGCTTTGCGGCCAATGCGCGGGAAGGGGAAACACAAAACCCACCGGCATAAAAAAGCCCGCTGCTACGGGGGGT‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCt                                                                                        <  1:267/148‑1 (MQ=255)
                                                          cTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGCTTTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCt                                                                                        <  1:101176/149‑1 (MQ=255)
                                                          cTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCt                                                                                        <  8:91158/148‑1 (MQ=255)
                                                           tCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTgg                                                                                      >  3:197858/1‑149 (MQ=255)
                                                            cgcgcgCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGc                                                                                     <  2:300165/149‑1 (MQ=255)
                                                             gcgcgcTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGct                                                                                    >  3:118999/1‑149 (MQ=255)
                                                             gcgcgcTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGc                                                                                     <  8:62880/148‑1 (MQ=255)
                                                             gcgcgcTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGc                                                                                     <  5:226209/148‑1 (MQ=255)
                                                                 gctggcgctgACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGcttctt                                                                                >  1:18206/1‑149 (MQ=255)
                                                                  ctggcgctgACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTg                                                                               <  3:162356/149‑1 (MQ=255)
                                                                     tcgctgACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACAAAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGc                                                                            <  4:405867/148‑1 (MQ=255)
                                                                     gcgctgACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTggg                                                                             <  4:404515/148‑1 (MQ=255)
                                                                       gctgACGGGGGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCgg                                                                          <  2:7825/149‑1 (MQ=255)
                                                                       gctgACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCgg                                                                          <  6:118687/149‑1 (MQ=255)
                                                                             gggAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACTCAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGc                                                                    >  2:280648/1‑149 (MQ=255)
                                                                             gggAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCAGAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGc                                                                    <  4:197858/149‑1 (MQ=255)
                                                                                  ccGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCa                                                               >  5:335792/1‑149 (MQ=255)
                                                                                  ccGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCa                                                               >  8:299550/1‑149 (MQ=255)
                                                                                   cGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCAc                                                              >  8:9520/1‑149 (MQ=255)
                                                                                           ggCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGATGGCCt                                                      >  8:74014/1‑149 (MQ=255)
                                                                                            gCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTc                                                     <  2:273330/149‑1 (MQ=255)
                                                                                            gCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTc                                                     >  6:211339/1‑149 (MQ=255)
                                                                                             cccATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTc                                                     >  3:213730/1‑148 (MQ=255)
                                                                                              ccATTCTCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCTTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCAACCACGTTGGCCTCGa                                                   <  6:217788/149‑1 (MQ=255)
                                                                                                 ttCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGACTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACt                                                 >  2:435096/1‑148 (MQ=255)
                                                                                                    gcgGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGTTAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCt                                             <  8:310703/149‑1 (MQ=255)
                                                                                                    gcgGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGggcgg                                                                                                          <  1:122967/88‑1 (MQ=255)
                                                                                                    gcgGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGggcgg                                                                                                          >  2:122967/1‑88 (MQ=255)
                                                                                                    gcgGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTcc                                              <  1:194040/148‑1 (MQ=255)
                                                                                                      gggcacgggcaACACAAACCCCCCAGCCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCCGCCACTCCGATCGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTGGGCCTCGACTTCCTGc                                           <  2:285745/149‑1 (MQ=255)
                                                                                                        gcacgggcaACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCAc                                         >  8:204979/1‑149 (MQ=255)
                                                                                                          acgggcaACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCt                                       >  8:149294/1‑149 (MQ=255)
                                                                                                           cgggcaACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCtt                                      <  4:118999/149‑1 (MQ=255)
                                                                                                             ggcaACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGTGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTgc                                    <  2:204323/149‑1 (MQ=255)
                                                                                                              gcaACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTgcc                                   >  3:136884/1‑149 (MQ=255)
                                                                                                              gcaACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTgcc                                   <  5:210553/149‑1 (MQ=255)
                                                                                                                  cacaAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGccgccg                               >  3:265404/1‑149 (MQ=255)
                                                                                                                      aaaCCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCgag                           <  2:121830/149‑1 (MQ=255)
                                                                                                                          ccACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAg                       >  3:259965/1‑149 (MQ=255)
                                                                                                                           cacaGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAg                       >  2:211597/1‑148 (MQ=255)
                                                                                                                            acaGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAgg                      >  6:399045/1‑148 (MQ=255)
                                                                                                                              aGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAAt                   <  6:150337/149‑1 (MQ=255)
                                                                                                                               ggCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAAtt                  <  2:18206/149‑1 (MQ=255)
                                                                                                                                   tAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTTCACCTTGCCGCCGCGAGAAAGGAAttcttc              <  7:299550/149‑1 (MQ=255)
                                                                                                                                   tAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAAttcttc              >  7:147781/1‑149 (MQ=255)
                                                                                                                                   tAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAAttcttc              <  7:149294/149‑1 (MQ=255)
                                                                                                                                    aaaaaaGCCCGCCGTCGCTGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGaa                    >  6:224693/1‑142 (MQ=255)
                                                                                                                                    aaaaaaGCCCGCCGTCGCTGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGaa                    <  5:224693/142‑1 (MQ=255)
                                                                                                                                    aaaaaaGCCCGCCGTCGCTGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAAttcttc              >  1:65099/1‑148 (MQ=255)
                                                                                                                                     aaaaaGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAATTCTTCCa            >  7:275/1‑149 (MQ=255)
                                                                                                                                        aaGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGACTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAATTCTTCCATCg         <  1:435096/149‑1 (MQ=255)
                                                                                                                                         aGCCAGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAATTCTTCCATCGc        <  1:211597/149‑1 (MQ=255)
                                                                                                                                          gCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAATTCTTCCATCGcc       <  7:74014/149‑1 (MQ=255)
                                                                                                                                          gCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAATTCTTCCATCGcc       <  8:72921/149‑1 (MQ=255)
                                                                                                                                             cGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAATGAATTCTTCCATCGCCTTg    <  1:280648/149‑1 (MQ=255)
                                                                                                                                                aGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAATTCTTCCATCGCCTTGGc  >  2:253131/1‑148 (MQ=255)
                                                                                                                                                aGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAATTCTTCCATCGCCTTGGc  >  5:234322/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GACTGGCGATCAGGCGCCGGATGCCGAAATCCAGCGCCTGTCGCAGCTGCCCGACGTGCTCGCGCGCTGGCGCTGACGGGAGCCGCTTTGCGGCCCATTCGCGGGCACGGGCAACACAAAACCCACAGGCATAAAAAAGCCCGCAGCGACGGCGGGC‑TTTTTTGCGATCAGCCACTCAGATAGGGCGGCTGCCGTACTTGTTGTCTGGCTTCTTGGGCGGGTCAGCGACCACGTTGGCCTCGACTTCCTGCACCTTGCCGCCGCGAGAAAGGAATTCTTCCATCGCCTTGGC  >  NC_002947/5965427‑5965718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑