| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,967,628 | A→C | 5.1% | L22R (CTG→CGG) | amtB ← | ammonium transporter AmtB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,967,628 | 0 | A | C | 5.1% | 312.3 / 6.6 | 98 | L22R (CTG→CGG) | amtB | ammonium transporter AmtB |
| Reads supporting (aligned to +/- strand): ref base A (42/51); new base C (3/2); total (45/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 | |||||||||||
ATCATCACCGACAGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCGGTACGGATGC > NC_002947/5967481‑5967776 | atcatcACCGACAGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAg < 6:302109/149‑1 (MQ=255)atcatcACCGACAGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAg < 5:179081/149‑1 (MQ=255) tcatcaCCGACAGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGACCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAgg > 1:326474/1‑149 (MQ=255) tcaCCGACAGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGc > 2:65218/1‑148 (MQ=255) caCCGACAGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGc > 2:156418/1‑147 (MQ=255) ccGACAGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGc > 7:166905/1‑149 (MQ=255) cAGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCgg < 7:13174/149‑1 (MQ=255) aGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCggg > 8:211465/1‑149 (MQ=255) aGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCggg < 1:394101/149‑1 (MQ=255) aGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCggg < 4:426967/149‑1 (MQ=255) gCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCCGGGCTTGC‑CCTGGc < 7:223180/149‑1 (MQ=255) ttCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTAc > 5:302717/1‑149 (MQ=255) ggATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGa < 8:13590/146‑1 (MQ=255) ggATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAg < 7:216332/147‑1 (MQ=255) tCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGAc > 1:168288/1‑148 (MQ=255) cACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCAGCAGCGGCGTTGCGGTCAGCATGCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGAGAATa < 2:233897/149‑1 (MQ=255) cACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACaa > 5:417805/1‑147 (MQ=255) cACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACaa > 6:233032/1‑147 (MQ=255) cACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATa < 2:168288/149‑1 (MQ=255) aCCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAg > 4:234171/1‑149 (MQ=255) ccgccATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTcc > 6:23275/1‑149 (MQ=255) ccgccATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTc > 6:17130/1‑148 (MQ=255) ccgccATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTc < 3:399691/148‑1 (MQ=255) gAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTg < 1:424291/149‑1 (MQ=255) aaCAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGc < 3:423667/149‑1 (MQ=255) aaCAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTAGGAGCCCtat > 4:87400/1‑147 (MQ=255) cAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGa < 8:96724/149‑1 (MQ=255) cAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGa < 1:65218/149‑1 (MQ=255) aGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGAt < 3:53335/149‑1 (MQ=255) gggCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTAGGAGCCCtattgtc < 3:87400/149‑7 (MQ=255) ggCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCt > 4:295462/1‑149 (MQ=255) ggCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCt < 5:23275/149‑1 (MQ=255) ggCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCt < 5:17130/149‑1 (MQ=255) ccAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTa > 8:338098/1‑149 (MQ=255) cAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTAc < 3:234171/149‑1 (MQ=255) cAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTAc < 1:99815/149‑1 (MQ=255) aaGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACg < 7:211465/149‑1 (MQ=255) cGGAATATTCATTTAAAGCACCACGGCCGTTGCGGTTATGATCCAGGCGGGGTCGCCGGAATTAATCGCTGGGGGATCTTCCTCTTGCATGGCAAGC‑CCGGGCATTAAGATGGACAATACGGCTCCTAGCCCTGCGATCTTACGCagag < 8:379327/149‑1 (MQ=255) gAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTc < 3:295462/149‑1 (MQ=255) aTAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCAt > 7:403457/1‑149 (MQ=255) aTAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCAt < 4:19190/149‑1 (MQ=255) tAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCAtg < 7:338098/149‑1 (MQ=255) tAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCAtg > 4:309456/1‑149 (MQ=255) aGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCAtgt > 6:182484/1‑149 (MQ=255) tCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCAtgttg > 3:378456/1‑149 (MQ=255) tCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCAtgttg > 1:177002/1‑149 (MQ=255) aTGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCagag > 4:124185/1‑139 (MQ=255) aTGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCagag < 3:124185/139‑1 (MQ=255) gAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGtttt > 7:39671/1‑149 (MQ=255) aaCAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTtc > 5:352321/1‑149 (MQ=255) cAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTtctc < 2:352600/149‑1 (MQ=255) gCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCATGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCt < 5:298759/149‑1 (MQ=255) gCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCt > 4:104144/1‑149 (MQ=255) cACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTg > 1:69784/1‑149 (MQ=255) cACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTg > 7:124478/1‑149 (MQ=255) ccAGTGCCGTTGCGGTCAGCATCCAGGCGGTGTCGGCGGTATTAAGCGCTGGGGGAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTggg < 8:371130/149‑1 (MQ=255) aGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGc < 3:309456/149‑1 (MQ=255) cGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTgg < 8:3339/149‑1 (MQ=255) cGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTgg < 8:166905/149‑1 (MQ=255) gCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGtt < 6:302717/148‑1 (MQ=255) cGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGttt < 4:194339/148‑1 (MQ=255) cGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTg < 4:378456/149‑1 (MQ=255) cGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTg < 2:326474/149‑1 (MQ=255) cGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTg < 3:104144/149‑1 (MQ=255) gTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTttggtttggt > 2:169194/1‑149 (MQ=255) gTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGt > 6:266907/1‑149 (MQ=255) gTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGt < 8:403457/149‑1 (MQ=255) gTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGt < 6:352321/149‑1 (MQ=255) aGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGa < 7:91491/148‑1 (MQ=255) aGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGa < 4:277145/148‑1 (MQ=255) aGCATCCAGGCGGTGTCACCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAg < 7:209732/149‑1 (MQ=255) cATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGc > 7:109496/1‑149 (MQ=255) aTCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTtctc < 4:365988/124‑1 (MQ=255) aTCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTtctc > 3:365988/1‑124 (MQ=255) tCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCtt > 1:285418/1‑149 (MQ=255) ggCGGGGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCtttttt < 5:43015/149‑1 (MQ=255) tgtCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTtgctgc > 7:288667/1‑149 (MQ=255) gCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTCCGCAGAGTCATGTTGTTTTCTCGTGGGGCGTTGGGTTTGGTGAGGCTTTTTTgctgct > 4:320964/1‑146 (MQ=255) gCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGt > 2:308015/1‑129 (MQ=255) gCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGAACTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGt < 1:308015/129‑1 (MQ=255) cccccaaTTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGTCAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTa < 1:156418/143‑1 (MQ=255) aaTTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATcgc > 3:96381/1‑149 (MQ=255) aTTAATCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGATGGAAAATAGGGATCCTAGCCCTGCGATCTTACGAAGAGTCAGGTTGGTTTCTCCTCGCGCGTTGGGATTGGCGAGGATTTTTTTCTGCTTAGATcgc > 2:263173/1‑148 (MQ=255) ttAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGt < 6:43998/149‑1 (MQ=255) ttAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGt < 8:288667/149‑1 (MQ=255) aaGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGATGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCg > 2:377761/1‑149 (MQ=255) aGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCg < 8:124478/148‑1 (MQ=255) tGGGGCAGCTTCCTCTGCCAGGGCAAGCTCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATc > 1:248034/1‑149 (MQ=255) aGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCg > 1:391386/1‑149 (MQ=255) cTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGcc < 5:25008/149‑1 (MQ=255) ccTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCgg > 6:390101/1‑148 (MQ=255) tctGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGt > 7:335206/1‑128 (MQ=255) tctGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGt < 8:335206/128‑1 (MQ=255) atGCCCGGGCTTGC‑CCTGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCGGTACg > 4:309617/2‑149 (MQ=255) tGCCCGGGCTTGC‑CCTGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCttttt < 2:339403/109‑1 (MQ=255) tGCCCGGGCTTGC‑CCTGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCttttt > 1:339403/1‑109 (MQ=255) gCCCGGGCTTGC‑CCTGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCGGTACGGa > 3:188330/1‑149 (MQ=255) cAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCGGTACGGATg > 6:171666/1‑149 (MQ=255) aGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCGGTACGGATGc < 6:417805/149‑1 (MQ=255) aGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCGGTACGGATGc < 5:266907/149‑1 (MQ=255) | ATCATCACCGACAGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGGGCAGCTTCCTCTGCCAGGGCAAGC‑CCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCGGTACGGATGC > NC_002947/5967481‑5967776 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |