Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,971,291	C→G	5.3%	M1M (ATG→ATC) †	PP_5236 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,971,291	0	C	G	5.3%	306.0 / 6.0	95	M1M (ATG→ATC) †	PP_5236	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (43/46);  new base G (2/3);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

ACTGAGCCTCGAGCCTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACTTGTTGCGCCGGCGTTGG  >  NC_002947/5971146‑5971438                                                                                                                                                    |                                                                                                                                                    aCTGAGCCTCGAGCCTGTCGAAAAAGCGGTTGAGTTCGACTAATGCACTGGCCTGCACCAGTT‑CGCGGATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATg                                                                                                                                                  <  5:88812/149‑1 (MQ=255) aCTGAGCCTCGAGCCTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATg                                                                                                                                                  <  5:438089/149‑1 (MQ=255) aCTGAGCCTCGAGCCTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATg                                                                                                                                                  <  7:192921/149‑1 (MQ=255)   tGAGCATCGAGCCTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCAGTTGAAAGAAGACGCAG‑TGACATCCCATgg                                                                                                                                                 >  1:432800/1‑148 (MQ=255)       ccTCGAGCCTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATGGGATGGCactg                                                                                                                                            >  5:387826/1‑146 (MQ=255)       ccTCGAGCCTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATGGGATGGCactg                                                                                                                                            <  6:387826/149‑4 (MQ=255)        cTCGAGCCTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTg                                                                                                                                           <  6:158474/149‑1 (MQ=255)         tCGAGCCTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CACGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGc                                                                                                                                          >  5:87218/1‑149 (MQ=255)          cGAGCCTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCATTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGcc                                                                                                                                         >  3:258128/1‑148 (MQ=255)           gAGCCTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCgg                                                                                                                                        >  7:70733/1‑149 (MQ=255)           gAGCCTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCgg                                                                                                                                        <  5:218157/149‑1 (MQ=255)               cTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTAc                                                                                                                                    <  3:4560/149‑1 (MQ=255)               cTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTAc                                                                                                                                    <  6:367588/149‑1 (MQ=255)                tGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTAcc                                                                                                                                   >  8:116053/1‑149 (MQ=255)                 gTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAC‑TGCCATCCCATGGCAACTGCGGTTAcc                                                                                                                                   >  6:335396/1‑148 (MQ=255)                  tCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAGTTGCCATGGGATGGC‑ACTGCgtcttct                                                                                                                                   <  2:53891/147‑7 (MQ=255)                  tCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATg                                                                                                                                                  >  4:346935/1‑132 (MQ=255)                  tCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATg                                                                                                                                                  <  3:346935/132‑1 (MQ=255)                  tCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTAccc                                                                                                                                  >  3:153168/1‑148 (MQ=255)                  tCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTAcc                                                                                                                                   <  7:332235/147‑1 (MQ=255)                    gAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTTGGGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCAATTTGAAAGAAGACGCAG‑TGCCATCCAATGGCAACTGCGGTTACCcaa                                                                                                                                >  1:309159/1‑149 (MQ=255)                    gAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAGGAAGACCAAT‑GGCAAACCCAGGCAAAGTGCGGTTACCcaa                                                                                                                                >  2:387005/1‑148 (MQ=255)                    gAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCcaa                                                                                                                                >  7:42847/1‑148 (MQ=255)                      aaaaGCGGTTGAGTTCGACGAATGCTCTGGCCTGCACCAGTT‑CGCGCATTACCTGGGGCGTGCGGGCGAGCGGTTGCACGGATCAATACCTCAAGTGCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTAGCcaacaa                                                                                                                             <  6:277315/149‑1 (MQ=255)                            ggTTGAGTTCGACGAATGCACTTGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTTCGGGCTAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTaa                                                                                                                       <  2:309159/149‑1 (MQ=255)                            ggTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTaa                                                                                                                       <  3:120734/149‑1 (MQ=255)                             gTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAg                                                                                                                      <  3:230058/149‑1 (MQ=255)                                gAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTc                                                                                                                    <  7:151808/148‑1 (MQ=255)                                   ttCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATAc                                                                                                                >  4:403414/1‑149 (MQ=255)                                   ttCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATAc                                                                                                                <  7:218951/149‑1 (MQ=255)                                   ttCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATAc                                                                                                                >  5:42786/1‑149 (MQ=255)                                       acgaATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGa                                                                                                            <  4:285431/149‑1 (MQ=255)                                        cgaATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGAc                                                                                                           >  5:267034/1‑149 (MQ=255)                                          aaTACACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGAGTTTGCACGGAGCAATACCTCAAGTACAATTGAAAGAAGACGCAG‑TGACATCCCATGGCAAATGCGGTTACCCAACAACACTAAGTCATACCTGACg                                                                                                          >  7:103338/1‑148 (MQ=255)                                           aTGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGcc                                                                                                        >  2:261104/1‑149 (MQ=255)                                            tGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCt                                                                                                       <  8:42847/149‑1 (MQ=255)                                               aCTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGTGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTgcg                                                                                                    <  8:67108/149‑1 (MQ=255)                                                     cTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGc                                                                                              >  2:265888/1‑149 (MQ=255)                                                         aCCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAGTTGCCATGGGATGGC‑ACTGCgtcttc                                                                                                                                    >  6:420526/1‑102 (MQ=37)                                                         aCCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAGTTGCCATGGGATGGC‑ACTGCgtcttc                                                                                                                                    <  5:420526/107‑6 (MQ=37)                                                         aCCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTAt                                                                                          >  6:324303/1‑149 (MQ=255)                                                         aCCAGTT‑CGCGCATTACCTGGTGAGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTTCGGTTCCCCAACAACACTAAGTCATACCTGACTCATGCGCAAAGCCTAt                                                                                          >  8:133280/1‑149 (MQ=255)                                                          ccAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTAtt                                                                                         <  5:324303/149‑1 (MQ=255)                                                            aGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTgg                                                                                       <  6:267034/149‑1 (MQ=255)                                                             gTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGa                                                                                      >  3:276130/1‑149 (MQ=255)                                                               t‑CGCGCATTACCAGGTGCGTGCGGGCGAGCGTTTGCACGTAGGAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGAt                                                                                     <  8:139673/148‑1 (MQ=255)                                                                   cgcATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCAt                                                                                 <  1:261104/149‑1 (MQ=255)                                                                       ttACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCAttttt                                                                             >  6:151303/1‑149 (MQ=255)                                                                       ttACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCAttttt                                                                             >  3:122083/1‑149 (MQ=255)                                                                          ccctgggcgtgggGGCGTCCGTTTTGAAGGACCAATTCCTCCATGTCATTTTAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGc                                                                           <  1:387005/142‑1 (MQ=255)                                                                          ccTGGTGCGTGCGGGAGAGAGTTTGCACGGATCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGcc                                                                          <  4:258128/149‑1 (MQ=255)                                                                                  gtgcgGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCa                                                                  <  6:87218/149‑1 (MQ=255)                                                                                   tgcgGGCGAGCGTTTGCACGGAGCAATAGCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCAt                                                                 >  1:246110/1‑149 (MQ=255)                                                                                   tgcgGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCAt                                                                 >  6:292476/1‑149 (MQ=255)                                                                                     cgGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATga                                                               <  3:423553/149‑1 (MQ=255)                                                                                         cGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGAcc                                                           <  6:429396/149‑1 (MQ=255)                                                                                         cGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTATTGCCGAACCGCATGAGAcc                                                           >  3:171506/1‑149 (MQ=255)                                                                                            gCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCa                                                        <  6:42786/149‑1 (MQ=255)                                                                                                ttGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATga                                                               >  1:422694/1‑138 (MQ=255)                                                                                                ttGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATga                                                               <  2:422694/138‑1 (MQ=255)                                                                                                 tGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGa                                                    <  4:276130/148‑1 (MQ=255)                                                                                                    aCGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATg                                                                >  8:374409/1‑133 (MQ=255)                                                                                                    aCGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATg                                                                <  7:374409/133‑1 (MQ=255)                                                                                                    aCGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACAGCATGAGACCGCAGGGaaaca                                                >  3:35510/1‑147 (MQ=255)                                                                                                     ctgAGCAATACCTCAAGTCCATTTGAAATAAGACGCAG‑TTCCATCCCATGGCAACTGCGGTTACCCAACAACACTAATTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAAcc                                               <  2:432800/147‑1 (MQ=255)                                                                                                          cAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAAccc                                              <  6:121901/145‑1 (MQ=255)                                                                                                          cAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAAccc                                              >  5:121901/1‑145 (MQ=255)                                                                                                           aaTACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCAt                                                                 >  7:99971/1‑125 (MQ=255)                                                                                                           aaTACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCAt                                                                 <  8:99971/125‑1 (MQ=255)                                                                                                           aaTACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACccctgcc                                         <  7:116053/149‑1 (MQ=255)                                                                                                             tACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACccctgccct                                       >  3:428771/1‑149 (MQ=255)                                                                                                               ccTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACccctgccctg                                      >  1:61190/1‑148 (MQ=255)                                                                                                               ccTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGa                                     <  5:335396/149‑1 (MQ=255)                                                                                                                 tCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGaa                                    >  3:326805/1‑148 (MQ=255)                                                                                                                 tCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGaa                                    >  8:81227/1‑148 (MQ=255)                                                                                                                    aGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGCGACCGCAGGGAAAACCCCTGCCCTGAAAAAc                                <  1:265888/149‑1 (MQ=255)                                                                                                                    aGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAAc                                <  2:22501/149‑1 (MQ=255)                                                                                                                         aTTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCacga                           >  4:40440/1‑149 (MQ=255)                                                                                                                          tttGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCacgac                          >  1:414561/1‑149 (MQ=255)                                                                                                                             gAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGca                       >  8:218784/1‑149 (MQ=255)                                                                                                                              aaaGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAAc                                                >  1:167271/1‑123 (MQ=255)                                                                                                                              aaaGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAAc                                                <  2:167271/123‑1 (MQ=255)                                                                                                                                 gaagaCGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACt                   >  4:329307/1‑149 (MQ=255)                                                                                                                                 gaagaCGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACt                   >  8:111798/1‑149 (MQ=255)                                                                                                                                   agaCTCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACttg                 <  7:133280/149‑1 (MQ=255)                                                                                                                                   agaCGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACttg                 <  2:61190/149‑1 (MQ=255)                                                                                                                                       gCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACttgtt               <  3:427451/147‑1 (MQ=255)                                                                                                                                       gCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACttgtt               >  4:427451/1‑147 (MQ=255)                                                                                                                                       gCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACTTGTTgc             <  3:403414/149‑1 (MQ=255)                                                                                                                                         aG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACccctgccct                                       <  4:253958/121‑1 (MQ=255)                                                                                                                                          g‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACTTGTTGCGcc          >  8:55630/1‑149 (MQ=255)                                                                                                                                          g‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACTTGTTGCGcc          >  5:344188/1‑149 (MQ=255)                                                                                                                                            tGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACTTGTTGCGCCg         <  4:122083/149‑1 (MQ=255)                                                                                                                                             gCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACTTGTTGCGCCgg        >  6:269273/1‑149 (MQ=255)                                                                                                                                                aTCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACTTGTTGCGCCGGCGt     <  5:269273/149‑1 (MQ=255)                                                                                                                                                   ccATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACTTGTTGCGCCGGCGTTgg  <  8:70733/149‑1 (MQ=255)                                                                                                                                                    cATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCGTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACTTGTTGCGCCGGCGTTgg  >  1:307051/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ACTGAGCCTCGAGCCTGTCGAAAAAGCGGTTGAGTTCGACGAATGCACTGGCCTGCACCAGTT‑CGCGCATTACCTGGTGCGTGCGGGCGAGCGTTTGCACGGAGCAATACCTCAAGTCCATTTGAAAGAAGACGCAG‑TGCCATCCCATGGCAACTGCGGTTACCCAACAACACTAAGTCATACCTGACGCCTGCGCAAAGCCTATTGGATCCATTTTTGCCGAACCGCATGAGACCGCAGGGAAAACCCCTGCCCTGAAAAACCACGACGCACACTTGTTGCGCCGGCGTTGG  >  NC_002947/5971146‑5971438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑