Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 92,186 T→C 5.0% Q65R (CAG→CGG) ‡ trpA ← tryptophan synthase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294792,1860TC 5.0% 301.2 / 4.0 99Q65R (CAG→CGG) ‡trpAtryptophan synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (51/43);  new base C (2/3);  total (53/46)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.89e-01

GTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGAGC  >  NC_002947/92042‑92333
                                                                                                                                                   |                                                                                                                                                    
gtgtCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGAt                                                                                                                                                  >  7:143505/1‑149 (MQ=255)
     cACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCATCCAGCTGGCCa                                                                                                                                             <  2:136280/149‑1 (MQ=255)
     cACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGAACGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCa                                                                                                                                             >  1:89800/1‑149 (MQ=255)
       cGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCATCCAGCTGGCCAcc                                                                                                                                           <  6:137608/149‑2 (MQ=255)
        gCCAAAGCGGTGGATAGGGTTGTAGTAGACCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgc                                                                                                                                          >  6:348909/1‑149 (MQ=255)
         ccAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgcc                                                                                                                                         <  3:90481/149‑1 (MQ=255)
                         ggTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTc                                                                                                                          <  2:212982/148‑1 (MQ=255)
                         ggTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTc                                                                                                                          >  1:212982/1‑148 (MQ=255)
                         ggTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCg                                                                                                                         >  3:354472/1‑149 (MQ=255)
                         ggTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCg                                                                                                                         <  2:330815/149‑1 (MQ=255)
                          gTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTAGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCg‑g                                                                                                                       >  8:19099/1‑149 (MQ=255)
                             gtagtaGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTg                                                                                                                     <  2:320184/148‑1 (MQ=255)
                              tagtagCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACCTCTTAATATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCg‑g                                                                                                                       <  1:364536/148‑1 (MQ=255)
                                   gCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGc                                                                                                               <  1:321591/148‑1 (MQ=255)
                                   gCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGc                                                                                                               <  5:121547/148‑1 (MQ=255)
                                    cccATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgcc                                                                                                                                         <  3:326509/122‑1 (MQ=255)
                                    cccATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgcc                                                                                                                                         >  4:326509/1‑122 (MQ=255)
                                    cccATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGGCATCGGGTCG‑GTGAACGGCAt                                                                                                             >  4:293802/1‑149 (MQ=255)
                                     ccATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATg                                                                                                            >  1:259222/1‑149 (MQ=255)
                                       aTCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCTCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCTCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAAc                                                                                                                  <  8:219863/141‑1 (MQ=255)
                                       aTCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAAc                                                                                                                  >  7:219863/1‑141 (MQ=255)
                                       aTCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGcc                                                                                                          <  8:412580/149‑1 (MQ=255)
                                       aTCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGcc                                                                                                          >  1:215968/1‑149 (MQ=255)
                                           gCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGc                                                                                                      <  8:143505/149‑1 (MQ=255)
                                            cACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGCCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGACG‑GTGAACGGCATGCCCAGCt                                                                                                     >  4:37591/1‑149 (MQ=255)
                                              cGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCg                                                                                                   <  3:282517/149‑1 (MQ=255)
                                                aTCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGAt                                                                                                 <  2:89800/149‑1 (MQ=255)
                                                   ggCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCATCCAGCTGGCCAccct                                                                                                                                         >  8:66070/1‑104 (MQ=255)
                                                   ggCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgccatcggccatc                                                                                                                               >  2:158255/1‑117 (MQ=255)
                                                   ggCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgccatcggccatc                                                                                                                               <  1:158255/117‑1 (MQ=255)
                                                   ggCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGCTGGCCATCCAGCTGGCCAccct                                                                                                                                         <  7:66070/107‑4 (MQ=255)
                                                    gCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACg                                                                                             >  8:311003/1‑149 (MQ=255)
                                                     cGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGc                                                                                                               <  5:421216/130‑1 (MQ=255)
                                                     cGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGc                                                                                                               >  6:421216/1‑130 (MQ=255)
                                                      gTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTc                                                                                           <  2:76547/149‑1 (MQ=255)
                                                       tGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCg                                                                                          >  7:49919/1‑149 (MQ=255)
                                                       tGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCg                                                                                          <  8:248922/149‑1 (MQ=255)
                                                        ggTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCgg                                                                                         >  3:79830/1‑149 (MQ=255)
                                                         gTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCgg                                                                                         <  4:79830/148‑1 (MQ=255)
                                                          tCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGgcg                                                                                       >  6:264683/1‑149 (MQ=255)
                                                           cTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGgcgc                                                                                      >  6:286793/1‑149 (MQ=255)
                                                              gTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGgcgc                                                                                      <  4:126404/146‑1 (MQ=255)
                                                              gTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCcag                                                                                   >  7:278291/1‑149 (MQ=255)
                                                                    ca‑caCGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGACAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCg                                                                             >  4:150849/1‑149 (MQ=255)
                                                                     a‑cacGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCAc                                                                                              <  3:70460/131‑1 (MQ=255)
                                                                     a‑cacGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCAc                                                                                              >  4:70460/1‑131 (MQ=255)
                                                                           gA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGc                                                                       <  5:348909/149‑1 (MQ=255)
                                                                                ttCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCt                                                                    >  1:402732/1‑149 (MQ=255)
                                                                                   gcgAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCGACGCCATCGGGCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCGttta                                                                 <  6:2075/149‑3 (MQ=255)
                                                                                   gcgAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCagcagc                                                                               >  3:173040/1‑135 (MQ=255)
                                                                                   gcgAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCagcagc                                                                               <  4:173040/135‑1 (MQ=255)
                                                                                       aCCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGAt                                                             <  7:311003/149‑1 (MQ=255)
                                                                                             tGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGCTCTGcca                                                       >  6:301054/1‑147 (MQ=255)
                                                                                               cAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGc                                                         <  3:331549/145‑1 (MQ=255)
                                                                                               cAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCaa                                                       <  8:49919/147‑1 (MQ=255)
                                                                                                 gtgTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCAACGGCCATCGGGGCG‑GTGCACGGCATGCCCAGCGCGAACACGACGGCGCCCGCAGCCGCCAGGCCGGGGAGGCACTGCAACgcg                                                   >  8:272514/3‑147 (MQ=255)
                                                                                                 gggTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCCCGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAg                                                   >  7:102742/1‑149 (MQ=255)
                                                                                                 gggTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGccc                                                                     >  7:188833/1‑131 (MQ=255)
                                                                                                 gggTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGccc                                                                     <  8:188833/131‑1 (MQ=255)
                                                                                                 gggTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAg                                                   <  2:259222/149‑1 (MQ=255)
                                                                                                  ggTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAgg                                                  >  4:130335/1‑149 (MQ=255)
                                                                                                    tCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcg                                                >  8:352904/1‑149 (MQ=255)
                                                                                                      ttGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcgtc                                              >  5:360180/1‑149 (MQ=255)
                                                                                                        gACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcgtc                                              <  6:265856/147‑1 (MQ=255)
                                                                                                           cAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGc                                         >  1:100961/1‑149 (MQ=255)
                                                                                                           cAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGc                                         <  5:301054/149‑1 (MQ=255)
                                                                                                           cAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGc                                         >  7:25263/1‑149 (MQ=255)
                                                                                                               gTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCgg                                     >  3:117497/1‑149 (MQ=255)
                                                                                                                tCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGCGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCgg                                     >  1:131890/1‑149 (MQ=255)
                                                                                                                  tGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTc                                  >  1:382171/1‑149 (MQ=255)
                                                                                                                  tGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTc                                  >  3:247407/1‑149 (MQ=255)
                                                                                                                    gtcAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTc                                  <  3:150849/145‑1 (MQ=255)
                                                                                                                    gCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGa                                                    >  1:295171/1‑129 (MQ=255)
                                                                                                                    gCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGa                                                    <  2:295171/129‑1 (MQ=255)
                                                                                                                    gCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTc                                  <  6:360180/147‑1 (MQ=255)
                                                                                                                    gCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGc                                >  8:257929/1‑149 (MQ=255)
                                                                                                                      cAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCGCGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCa                              <  4:358244/149‑1 (MQ=255)
                                                                                                                         cTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCagcagc                                                                               >  1:379142/1‑97 (MQ=255)
                                                                                                                         cTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCagcagc                                                                               <  2:379142/97‑1 (MQ=255)
                                                                                                                          ttCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGTCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCtg                          >  8:29497/1‑149 (MQ=255)
                                                                                                                           tCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCtgt                         >  4:297225/1‑149 (MQ=255)
                                                                                                                            cAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGTTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcgtcgt                                            <  3:18418/129‑1 (MQ=255)
                                                                                                                            cAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcgtcgt                                            >  4:18418/1‑129 (MQ=255)
                                                                                                                            cAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCtgt                         <  5:286793/148‑1 (MQ=255)
                                                                                                                             aaGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGa                       >  1:106004/1‑149 (MQ=255)
                                                                                                                               gggCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGAc                      >  4:308487/1‑148 (MQ=255)
                                                                                                                                 gcgcgcAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGaa                   <  2:100961/149‑1 (MQ=255)
                                                                                                                                  cgcgcAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAg                  <  4:354472/149‑1 (MQ=255)
                                                                                                                                  cgcgcAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAg                  <  2:402732/149‑1 (MQ=255)
                                                                                                                                    gatggcgTGGCCATCCAGCTGGCCACCCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGt                >  1:101107/7‑149 (MQ=255)
                                                                                                                                       aGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAATGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGAc             >  5:276621/1‑149 (MQ=255)
                                                                                                                                         ggTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCa           <  5:176375/149‑1 (MQ=255)
                                                                                                                                             gCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAATGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGc       <  6:276621/149‑1 (MQ=255)
                                                                                                                                             gCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGc       >  4:131507/1‑149 (MQ=255)
                                                                                                                                              ccAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGcc      >  1:128563/1‑149 (MQ=255)
                                                                                                                                              ccAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGAGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTgat      >  7:94952/1‑147 (MQ=255)
                                                                                                                                                aGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGa    <  2:215968/149‑1 (MQ=255)
                                                                                                                                                 gCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGAg   >  7:326472/1‑149 (MQ=255)
                                                                                                                                                  cTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGAGc  >  1:198335/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCA‑CACGGA‑‑ATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCG‑GTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGAGC  >  NC_002947/92042‑92333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑