Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,026,074 C→T 8.1% Q316* (CAA→TAA) ‡ PP_5276 → phospholipase D family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,026,0740CT 8.1% 251.0 / 7.9 86Q316* (CAA→TAA) ‡PP_5276phospholipase D family protein
Reads supporting (aligned to +/- strand):  ref base C (42/37);  new base T (2/5);  total (44/42)
Fisher's exact test for biased strand distribution p-value = 2.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.48e-01

TACGACCGCTTGATGGCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGCTGGAAGCC  >  NC_002947/6025927‑6026220
                                                                                                                                                   |                                                                                                                                                  
tACGACCGCTTGATGGCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGc                                                                                                                                                    >  4:361017/1‑148 (MQ=255)
   gACCGCTTGATGGCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACt                                                                                                                                                <  1:16475/149‑1 (MQ=255)
           gagggCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGTTGCTGGCTcag                                                                                                                                         <  6:177825/145‑3 (MQ=255)
           gATGGCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCgg                                                                                                                                        >  3:271432/1‑149 (MQ=255)
           gATGGCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCg                                                                                                                                         >  2:152133/1‑148 (MQ=255)
            aTGGCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGTTGCTGGCTcagca                                                                                                                                       <  1:47882/149‑5 (MQ=255)
            aTGGCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGTTGCTGGCTcagca                                                                                                                                       <  5:351689/149‑5 (MQ=255)
             tGGCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGTTGCTGGCTcagca                                                                                                                                       <  5:226314/148‑5 (MQ=255)
             tGGCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGTTGCTGGCTcag                                                                                                                                         >  6:226314/1‑144 (MQ=255)
              ggCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGAcc                                                                                                                                     >  3:26258/1‑149 (MQ=255)
               gCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGAGCt                                                                                                                                    >  4:63736/1‑149 (MQ=255)
               gCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCt                                                                                                                                    <  6:270658/149‑1 (MQ=255)
                      aGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAAc                                                                                                                             <  2:343470/149‑1 (MQ=255)
                      aGTCGCAGCCGCGACTGGATCTGTGGCGCAACGAGCTGATCTGGGCTCAAGCACAGGCCCTATGGGATGCGCCCAGCAAGCTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGATCCGGACCAGGCTAAc                                                                                                                             >  8:7403/1‑149 (MQ=255)
                        tCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGt                                                                                                                           >  2:253701/1‑149 (MQ=255)
                          gCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTg                                                                                                                          >  5:287775/1‑148 (MQ=255)
                           cAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCa                                                                                                                        <  8:133462/149‑1 (MQ=255)
                             gCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGcccc                                                                                                                      >  3:51479/1‑149 (MQ=255)
                              ccGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACcg                                                                                                                     <  6:287775/149‑1 (MQ=255)
                              ccGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACcg                                                                                                                     >  4:427120/1‑149 (MQ=255)
                               cgcgCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACcgc                                                                                                                    <  3:411611/149‑1 (MQ=255)
                                gcgcCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTc                                                                                                                                           >  2:334725/1‑125 (MQ=255)
                                gcgcCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTc                                                                                                                                           <  1:334725/125‑1 (MQ=255)
                                 cgcCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGTAACTGGCTCCGGACCTGGCTAACGTGCACCGCGa                                                                                                                  <  7:108359/149‑1 (MQ=255)
                                    cTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGtt                                                                                                               <  5:290899/149‑1 (MQ=255)
                                       gATGTGTGGGGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTg                                                                                                              <  1:152133/147‑1 (MQ=255)
                                           tgtgGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTg                                                                                                        <  8:332906/149‑1 (MQ=255)
                                            gtgGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTgg                                                                                                       >  2:216764/1‑149 (MQ=255)
                                               gcgcAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCAt                                                                                                    <  1:253701/149‑1 (MQ=255)
                                                 gcAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCg                                                                                                  >  7:49518/1‑149 (MQ=255)
                                                 gcAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCg                                                                                                  <  4:271432/149‑1 (MQ=255)
                                                   aaCGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGc                                                                                                >  3:322098/1‑149 (MQ=255)
                                                      gAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTa                                                                                             >  1:12570/1‑149 (MQ=255)
                                                       aGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTAc                                                                                            <  5:267212/149‑1 (MQ=255)
                                                       aGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTAc                                                                                            <  2:141543/149‑1 (MQ=255)
                                                        gCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACt                                                                                           >  8:97900/1‑149 (MQ=255)
                                                           gATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTg                                                                                        >  3:395816/1‑149 (MQ=255)
                                                            aTCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCgg                                                                                                                                        >  4:330080/1‑100 (MQ=255)
                                                            aTCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCgg                                                                                                                                        <  3:330080/100‑1 (MQ=255)
                                                            aTCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACttt                                                                                         <  3:63736/147‑1 (MQ=255)
                                                            aTCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACttt                                                                                         <  4:83017/147‑1 (MQ=255)
                                                            aTCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTg                                                                                        <  2:277035/148‑1 (MQ=255)
                                                                 ggCTCACTCACAGGCCATATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGt                                                                                                                           >  6:180225/1‑108 (MQ=255)
                                                                 ggCTCACGCACAGGCCATATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGt                                                                                                                           <  5:180225/108‑1 (MQ=255)
                                                                    tCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGc                                                                                >  2:289860/1‑148 (MQ=255)
                                                                          acaGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCgg                                                                         <  1:379218/149‑1 (MQ=255)
                                                                            aGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCgggg                                                                       <  4:26258/149‑1 (MQ=255)
                                                                                 cTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGgctgct                                                                  <  1:289860/149‑1 (MQ=255)
                                                                                      ggATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGa                                                                                                                                       >  6:228126/1‑75 (MQ=255)
                                                                                      ggATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGa                                                                                                                                       <  5:228126/75‑1 (MQ=255)
                                                                                      ggATGCGCCCAGCAAGGGGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTAc                                                              <  4:214829/148‑1 (MQ=255)
                                                                                         tGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGAGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGa                                                          >  5:69308/1‑149 (MQ=255)
                                                                                           cgcCGAGCATGGTGTTGGACGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCAGCGCGAGTTGGTCCTGGCATCTGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTTCCTGAc                                                         <  8:49518/148‑1 (MQ=255)
                                                                                            gcCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCg                                                       >  5:279951/1‑149 (MQ=255)
                                                                                            gcCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCg                                                       >  2:24591/1‑149 (MQ=255)
                                                                                                  cAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCcgcg                                                 >  4:307440/1‑149 (MQ=255)
                                                                                                            gCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTg                                        <  2:12570/148‑1 (MQ=255)
                                                                                                              cGAAGACGAACCGGCCCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCg                                     >  3:281447/1‑149 (MQ=255)
                                                                                                              cGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTAc                                                              >  6:268287/1‑124 (MQ=255)
                                                                                                              cGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTAc                                                              <  5:268287/124‑1 (MQ=255)
                                                                                                              cGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCg                                     <  7:97900/149‑1 (MQ=255)
                                                                                                               gAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGgctg                                                                    <  1:386933/117‑1 (MQ=255)
                                                                                                               gAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGgctg                                                                    >  2:386933/1‑117 (MQ=255)
                                                                                                                  gACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTc                                 >  8:38952/1‑149 (MQ=255)
                                                                                                                    cGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCgg                               <  2:359270/149‑1 (MQ=255)
                                                                                                                     gAACCGGACCCGCAATTACTGCTGAGCCTGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGTGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGTCGTTTCGGt                              <  7:7403/149‑1 (MQ=255)
                                                                                                                     gAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGt                              <  1:216764/149‑1 (MQ=255)
                                                                                                                     gAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGt                              >  5:414543/1‑149 (MQ=255)
                                                                                                                     gAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGt                              >  3:374573/1‑149 (MQ=255)
                                                                                                                              ccGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCgg                               <  7:317896/139‑1 (MQ=255)
                                                                                                                              ccGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCgg                               >  8:317896/1‑139 (MQ=255)
                                                                                                                                      aCTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACt             >  1:2678/1‑149 (MQ=255)
                                                                                                                                        tgctgAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCg           >  7:81583/1‑149 (MQ=255)
                                                                                                                                          ctgAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCg           <  6:279951/147‑1 (MQ=255)
                                                                                                                                          ctgAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGc          <  3:427120/148‑1 (MQ=255)
                                                                                                                                          ctgAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGCt         >  4:362475/1‑149 (MQ=255)
                                                                                                                                          ctgAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACAGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGAGTTTCGGTGAAGCTGCTGACCACCTCGCt         >  8:362232/1‑149 (MQ=255)
                                                                                                                                          cggAGCCAGTTGCTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGCt         >  3:124119/3‑149 (MQ=255)
                                                                                                                                            gAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGCTg        >  2:361272/1‑148 (MQ=255)
                                                                                                                                             aGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGCTgg       >  5:359439/1‑148 (MQ=255)
                                                                                                                                              gCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGCTgg       >  4:242492/1‑147 (MQ=255)
                                                                                                                                              gCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCAGCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGCTGGaa     <  3:361017/149‑1 (MQ=255)
                                                                                                                                               ccAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGCTGGAAg    >  4:84758/1‑149 (MQ=255)
                                                                                                                                               ccAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGCTGGAAg    >  7:238870/1‑149 (MQ=255)
                                                                                                                                                cAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGCTGGAAGc   >  5:337740/1‑149 (MQ=255)
                                                                                                                                                cAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGCTGGAAGc   <  2:2678/149‑1 (MQ=255)
                                                                                                                                                 aGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGCTGGAAGcc  >  8:122377/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TACGACCGCTTGATGGCCTATCAGTCGCAGCCGCGCCTGGATGTGTGGCGCAACGAGCTGATCTGGGCTCACGCACAGGCCCTATGGGATGCGCCCAGCAAGGTGTTGGCCGAAGACGAACCGGACCCGCAATTACTGCTGAGCCAGCAACTGGCTCCGGACCTGGCTAACGTGCACCGCGAGTTGGTCCTGGCATCGGCCTACTTTGTACCGGGCGAACCGGGGCTGCTGTACCTGACCGGCCGCGCCGACGCTGGCGTTTCGGTGAAGCTGCTGACCAACTCGCTGGAAGCC  >  NC_002947/6025927‑6026220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑