BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,042,337	G→C	5.1%	E92Q (GAA→CAA)	PP_5295 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,042,337	0	G	C	5.1%	392.1 / 8.8	118	E92Q (GAA→CAA)	PP_5295	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (66/46); new base C (3/3); total (69/49)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

Only 100 of 122 total aligned reads displayed.

CCGGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCAT  >  NC_002947/6042191‑6042484

ccGGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGaa                                                                                                                                                   <  2:306393/149‑1 (MQ=255)

     aGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCAc                                                                                                                                              >  4:352224/1‑149 (MQ=255)

         aaGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTg                                                                                                                                          >  5:318843/1‑149 (MQ=255)

         aaGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTg                                                                                                                                          <  4:120287/149‑1 (MQ=255)

              aCCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCt                                                                                                                                     >  8:41922/1‑149 (MQ=255)

                    gTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGa                                                                                                                               <  8:293699/149‑1 (MQ=255)

                     tCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGaa                                                                                                                              <  8:188366/149‑1 (MQ=255)

                     tCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGaa                                                                                                                              <  2:353986/149‑1 (MQ=255)

                     tCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGaa                                                                                                                              <  6:184897/149‑1 (MQ=255)

                       tGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTGGCGCCTCAAagaaga                                                                                                                            >  8:401358/1‑149 (MQ=255)

                        gggAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTgcc                                                                                                                                        >  3:354952/1‑135 (MQ=255)

                        gggAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTgcc                                                                                                                                        <  4:354952/136‑2 (MQ=255)

                         ggAGCTGCGGTCGGGCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACa                                                                                                                          <  2:339090/149‑1 (MQ=255)

                            gCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGc                                                                                                                        >  4:103582/1‑148 (MQ=255)

                                 gTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGc                                                                                                                  <  5:28655/149‑1 (MQ=255)

                                     ggTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCaaa                                                                                                               <  6:2477/148‑1 (MQ=255)

                                     ggTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCaaa                                                                                                               <  6:355651/148‑1 (MQ=255)

                                     ggTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAAc                                                                                                              <  8:40912/149‑1 (MQ=255)

                                      gTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAAcc                                                                                                             >  7:250932/1‑149 (MQ=255)

                                       tCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCAc                                                                                                                                              <  1:57420/115‑1 (MQ=255)

                                       tCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCAc                                                                                                                                              >  2:57420/1‑115 (MQ=255)

                                        cAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGc                                                                                                           >  6:223206/1‑149 (MQ=255)

                                         aaCCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCt                                                                                                          >  5:215148/1‑149 (MQ=255)

                                         aaCCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCt                                                                                                          <  3:103582/149‑1 (MQ=255)

                                           ccaccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTg                                                                                                         >  4:339084/1‑148 (MQ=255)

                                            caccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTg                                                                                                         <  6:44813/147‑1 (MQ=255)

                                            caccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTg                                                                                                         >  5:44813/1‑147 (MQ=255)

                                            caccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGa                                                                                                        >  5:421579/1‑148 (MQ=255)

                                             accGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGaaa                                                                                                      <  1:429978/149‑1 (MQ=255)

                                              ccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAg                                                                                                     >  4:25618/1‑149 (MQ=255)

                                              ccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCATCAACGAAGACAGCAATGGCAAACCGCTGAAAg                                                                                                     >  5:88153/1‑149 (MQ=255)

                                               cGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGt                                                                                                    <  8:52671/149‑1 (MQ=255)

                                                 ttATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTg                                                                                                   >  2:74256/1‑148 (MQ=255)

                                                 ttATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTg                                                                                                   >  4:190899/1‑148 (MQ=255)

                                                   aTCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgcgc                                                                                                                                       <  7:350170/110‑1 (MQ=255)

                                                   aTCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgcgc                                                                                                                                       >  8:350170/1‑110 (MQ=255)

                                                   aTCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGa                                                                                                 <  2:201810/148‑1 (MQ=255)

                                                      tGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCAt                                                                                             >  7:373194/1‑149 (MQ=255)

                                                        gAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCAt                                                                                             >  4:308465/1‑147 (MQ=255)

                                                          gCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAgc                                                                                         <  3:19975/149‑1 (MQ=255)

                                                            cGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAgcgc                                                                                       <  7:41922/149‑1 (MQ=255)

                                                             gCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGCCCATGAgcgcg                                                                                      >  3:306398/1‑149 (MQ=255)

                                                             gCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAgcgcg                                                                                      >  1:310773/1‑149 (MQ=255)

                                                             gCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAgcgcg                                                                                      <  6:352823/149‑1 (MQ=255)

                                                                 cTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGcgcgccgc                                                                                  <  3:339084/149‑1 (MQ=255)

                                                                  tGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAg                                                                                                     <  4:99512/129‑1 (MQ=255)

                                                                   gcgcCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGcgcgccgcg                                                                                 >  2:201380/1‑148 (MQ=255)

                                                                     gcCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCaa                                                                              <  2:310773/149‑1 (MQ=255)

                                                                      ccTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAAGCggt                                                                                                          >  2:174052/1‑118 (MQ=255)

                                                                      ccTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCt                                                                                                          <  1:174052/120‑1 (MQ=255)

                                                                      ccTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAAc                                                                             <  1:86815/149‑1 (MQ=255)

                                                                       cTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACt                                                                            <  7:377373/149‑1 (MQ=255)

                                                                         gCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTgg                                                                          >  1:376220/1‑149 (MQ=255)

                                                                         gCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTgg                                                                          >  4:213066/1‑149 (MQ=255)

                                                                          cccGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTg                                                                           <  4:39686/147‑1 (MQ=255)

                                                                          cccGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGt                                                                         >  3:117043/1‑149 (MQ=255)

                                                                           ccGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGtt                                                                        <  2:124459/149‑1 (MQ=255)

                                                                            cGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGcgcgccgcg                                                                                 >  6:86145/1‑139 (MQ=255)

                                                                                  ccTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTgccgccg                                                                 >  6:172356/1‑149 (MQ=255)

                                                                                  ccTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTgccgccg                                                                 >  7:307951/1‑149 (MQ=255)

                                                                                    tGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTgccgccgcc                                                               >  5:242117/1‑149 (MQ=255)

                                                                                      cgcgACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGa                                                             <  5:336801/149‑1 (MQ=255)

                                                                                        cgACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAg                                                            >  7:287984/1‑148 (MQ=255)

                                                                                         gACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAgg                                                           >  2:332420/1‑148 (MQ=255)

                                                                                            cTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGt                                                       <  3:352224/149‑1 (MQ=255)

                                                                                             tCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtg                                                      >  6:21140/1‑149 (MQ=255)

                                                                                              cGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtgg                                                     >  1:201307/1‑149 (MQ=255)

                                                                                                  ggCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtggctgg                                                 <  4:241687/149‑1 (MQ=255)

                                                                                                    cGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGcc                                               <  3:25618/149‑1 (MQ=255)

                                                                                                     ggTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCt                                              >  8:229393/1‑149 (MQ=255)

                                                                                                      gtgtACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTg                                             <  3:321331/149‑1 (MQ=255)

                                                                                                       tgtACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGa                                            <  3:213066/149‑1 (MQ=255)

                                                                                                        gtACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGCCCTGAt                                           >  6:169260/1‑149 (MQ=255)

                                                                                                         tACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGa                                                                                                                               >  6:298546/1‑64 (MQ=255)

                                                                                                         tACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGa                                                                                                                               <  5:298546/64‑1 (MQ=255)

                                                                                                         tACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGa                                                                                                                               <  3:52102/64‑1 (MQ=255)

                                                                                                         tACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGAt                                           <  2:146378/148‑1 (MQ=255)

                                                                                                              gAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAag                                       >  5:40749/1‑147 (MQ=255)

                                                                                                               aaGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtgg                                                     >  8:374541/1‑132 (MQ=255)

                                                                                                               aaGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtgg                                                     <  7:374541/132‑1 (MQ=255)

                                                                                                               aaGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAagca                                     <  4:117043/148‑1 (MQ=255)

                                                                                                                  gCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCg                                 >  6:384823/1‑149 (MQ=255)

                                                                                                                  gCCTGCGCCAGGACCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCg                                 >  6:45806/1‑149 (MQ=255)

                                                                                                                     tgtgcCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGcc                              >  3:282254/4‑149 (MQ=255)

                                                                                                                     tGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGcc                              >  5:57705/1‑149 (MQ=255)

                                                                                                                        gcCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGccccc                           >  3:106544/1‑149 (MQ=255)

                                                                                                                            gggCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGa                       >  4:228708/1‑149 (MQ=255)

                                                                                                                                cTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAAccc                   >  1:187791/1‑149 (MQ=255)

                                                                                                                                cTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAAccc                   >  2:262250/1‑149 (MQ=255)

                                                                                                                                   tCACGCGGCAAGGTGGAATGCAGAATGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCt                >  7:64998/1‑149 (MQ=255)

                                                                                                                                   tCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCt                <  1:351158/149‑1 (MQ=255)

                                                                                                                                     gaggcgCAAGGTGCATTCCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTg               >  3:52792/6‑148 (MQ=255)

                                                                                                                                     aCGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTg               >  5:299401/1‑148 (MQ=255)

                                                                                                                                       gcgGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGaa            <  6:318843/149‑1 (MQ=255)

                                                                                                                                          gCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTg         <  2:376220/149‑1 (MQ=255)

                                                                                                                                            aagggtgAATGCACCTTGCGCCTCAACGTAGACATCAATGGCAAACCGCTGTAAGTGGACCATGACCGCGCCGCGCAACTGGGTTCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCt       <  8:287984/143‑1 (MQ=255)

                                                                                                                                               gTGGAATACACCTTGCGCCTCAACGAAGACAGCAATGGAAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCAGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGt     >  5:234629/1‑148 (MQ=255)

                                                                                                                                                 ggAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCAt  >  4:75176/1‑149 (MQ=255)

                                                                                                                                                  gAATGGCCCTTGCGGCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCAt  <  8:365509/148‑1 (MQ=255)

                                                                                                                                                  gAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCAt  <  7:92874/148‑1 (MQ=255)

CCGGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCGCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCAT  >  NC_002947/6042191‑6042484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`