Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,067,735 G→T 5.3% S126S (TCG→TCT)  PP_5322 → metal ion transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,067,7350GT 5.3% 244.9 / 2.6 76S126S (TCG→TCT) PP_5322metal ion transporter
Reads supporting (aligned to +/- strand):  ref base G (33/38);  new base T (2/2);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

GGGCATCACCCTCGCCTCGCTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCC  >  NC_002947/6067588‑6067879
                                                                                                                                                     |                                                                                                                                                 
gggCATCACCCTCGCGTCGCTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCg                                                                                                                                                   <  8:297426/148‑1 (MQ=255)
 ggCATCACCCTCGCCTCGCTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGt                                                                                                                                                  <  3:102973/148‑1 (MQ=255)
 ggCATCACCCTCGCCTCGCTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTa                                                                                                                                                 <  1:341136/149‑1 (MQ=255)
  gCATCACCCTCGCCTCGCTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTAc                                                                                                                                                >  6:112042/1‑149 (MQ=255)
  gCATCACCCTCGCCGCGCTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGGCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTAc                                                                                                                                                <  5:342880/149‑1 (MQ=255)
   cATCACCCTCGCCTCGCTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTAcc                                                                                                                                               >  3:335178/1‑149 (MQ=255)
   cATCACCCTCGCCTCGCT‑GGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCt                                                                                                                                              <  7:67988/149‑1 (MQ=255)
    ataccccGCGCCTGGCTGGGCCGGGGGGGGGGGGGTTTGCCCGCCTTGGCCCAATTGTGGTAACCATTTCTGGCTTTCTCATCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTAc                                                                                                                                                <  1:136544/145‑1 (MQ=255)
     tCACCCTCGCCTCGCTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATCGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTg                                                                                                                                             >  2:212680/1‑149 (MQ=255)
      cagcctcgactcgATGGGCCTGGTCTGGGTGGGTGAGCCCGACTTCGCCCTCTTGCTGGATCCATTGCTGGC‑CTATC‑TGGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGACTTCTTCGTCATCTCGTACCTg                                                                                                                                             <  5:217778/145‑1 (MQ=255)
       aCCCTCGCCTCGCTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGc                                                                                                                                            <  2:415730/148‑1 (MQ=255)
         cctcgcctcgCTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGcaca                                                                                                                                         >  2:377144/1‑149 (MQ=255)
              ccgcgcTGGGCCTGGGCGGGGGGGTGGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTCTC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTg                                                                                                                                    <  8:407177/146‑1 (MQ=255)
               ctcgcTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATc                                                                                                                                       <  4:326673/145‑1 (MQ=255)
               ctcgcTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATc                                                                                                                                       >  3:326673/1‑145 (MQ=255)
               ctcgcTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTgg                                                                                                                                   >  5:183007/1‑149 (MQ=255)
               ctcgcTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTgg                                                                                                                                   <  7:146421/149‑1 (MQ=255)
                    tgggcctgggcTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGTACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCTTACCTGCACATCGTGGTCGGc                                                                                                                              >  1:306244/1‑149 (MQ=255)
                    tgggcctgggcTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCTTACCTGCACATCGTGGTCGGc                                                                                                                              >  7:125308/1‑149 (MQ=255)
                    tgggcctgggcTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGc                                                                                                                              <  5:112042/149‑1 (MQ=255)
                    ggggccggggcTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGc                                                                                                                              <  6:37439/148‑1 (MQ=255)
                       gcctgggcTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGaa                                                                                                                           >  7:210814/1‑149 (MQ=255)
                           gggctgggTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTgg                                                                                                                       >  2:263443/1‑149 (MQ=255)
                             gctgggTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGcc                                                                                                                     >  5:107950/1‑149 (MQ=255)
                                      gtgAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGc                                                                                                                              <  1:370063/131‑1 (MQ=255)
                                      gtgAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGc                                                                                                                              >  2:370063/1‑131 (MQ=255)
                                         aGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTgg                                                                                                         <  1:171094/149‑1 (MQ=255)
                                          gCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCGTggg                                                                                                        >  4:349559/1‑149 (MQ=255)
                                          gCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTggg                                                                                                        >  7:191871/1‑149 (MQ=255)
                                               ccTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATc                                                                                                   <  3:420356/149‑1 (MQ=255)
                                                   cGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGc                                                                                                >  8:345514/1‑148 (MQ=255)
                                                   cGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCCTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGc                                                                                                >  1:94439/1‑148 (MQ=255)
                                                             cTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCTTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAgc                                                                                     <  8:125308/148‑1 (MQ=255)
                                                             cTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCTTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAgc                                                                                     <  2:306244/148‑1 (MQ=255)
                                                              tGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAgctg                                                                                   >  5:437036/1‑149 (MQ=255)
                                                                gAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTATCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAgctgct                                                                                 >  8:253184/1‑149 (MQ=255)
                                                                gAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAgctgc                                                                                  <  1:377144/148‑1 (MQ=255)
                                                                      ttGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGGCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCt                                                                           <  5:254733/149‑1 (MQ=255)
                                                                        gCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCtg                                                                          <  8:191871/148‑1 (MQ=255)
                                                                          tGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCtgtg                                                                        >  3:414172/1‑148 (MQ=255)
                                                                            gC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCATCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCtgt                                                                         <  8:361913/145‑1 (MQ=255)
                                                                            gC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCATCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCtgt                                                                         >  7:361913/1‑145 (MQ=255)
                                                                             c‑cTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGAcc                                                                    >  7:17892/1‑149 (MQ=255)
                                                                               cTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTTCCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGAccg                                                                   >  5:183997/1‑149 (MQ=255)
                                                                               cTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGAccg                                                                   >  8:173145/1‑149 (MQ=255)
                                                                                   c‑tcGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTgg                                                                       >  8:332609/1‑141 (MQ=255)
                                                                                   c‑tcGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTgg                                                                       <  7:332609/141‑1 (MQ=255)
                                                                                       ggCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTg                                                              <  1:66822/147‑1 (MQ=255)
                                                                                             gACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTa                                                      <  2:65204/149‑1 (MQ=255)
                                                                                              aCAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTAc                                                     <  1:393690/149‑1 (MQ=255)
                                                                                                 gcgcCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTg                                                  <  4:335178/149‑1 (MQ=255)
                                                                                                  cgcCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGc                                                                            <  3:192973/122‑1 (MQ=255)
                                                                                                  cgcCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGc                                                                            >  4:192973/1‑122 (MQ=255)
                                                                                                  cgcCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAACTCCTGGGCTATCAGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGt                                                 >  1:104495/1‑149 (MQ=255)
                                                                                                            gTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTgg                                         <  1:263443/147‑1 (MQ=255)
                                                                                                             tCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAATGCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGc                                       <  1:332342/149‑1 (MQ=255)
                                                                                                               aaGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGAt                                    <  3:405319/149‑1 (MQ=255)
                                                                                                                aGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTAc                                                     <  3:206451/131‑1 (MQ=255)
                                                                                                                aGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTAc                                                     >  4:206451/1‑131 (MQ=255)
                                                                                                                   cGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTAc                                <  6:107950/149‑1 (MQ=255)
                                                                                                                   cGATCTCGTGCTTCGGCCCCTTCTTCGTCATCTCGTACCTGCACATCGTGGGCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTAc                                <  8:280290/149‑1 (MQ=255)
                                                                                                                         cGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGcc                          <  8:210814/149‑1 (MQ=255)
                                                                                                                          gTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGc                           <  6:437036/147‑1 (MQ=255)
                                                                                                                              ttcGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCg                                                           >  8:123202/1‑111 (MQ=255)
                                                                                                                              ttcGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTa                     >  3:342269/1‑149 (MQ=255)
                                                                                                                               tcgtcgCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATcgct                    >  7:382365/1‑146 (MQ=255)
                                                                                                                                cgtcgCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCgg                            >  3:11553/1‑140 (MQ=255)
                                                                                                                                cgtcgCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCgg                            <  4:11553/140‑1 (MQ=255)
                                                                                                                                 gtcgCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTAcc                   <  8:17892/148‑1 (MQ=255)
                                                                                                                                  tcgCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTg                 >  2:382655/1‑149 (MQ=255)
                                                                                                                                            cGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGTGATGTACCCGGCCATCTACCTGCTCAATGc         >  7:64022/1‑147 (MQ=255)
                                                                                                                                            cGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGc         >  3:344903/1‑147 (MQ=255)
                                                                                                                                            cGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGc         <  7:345514/147‑1 (MQ=255)
                                                                                                                                            cGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGc         >  8:338928/1‑147 (MQ=255)
                                                                                                                                             gTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCa       <  1:212680/148‑1 (MQ=255)
                                                                                                                                              tCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGc                <  7:24917/138‑1 (MQ=255)
                                                                                                                                              tCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGc                >  8:24917/1‑138 (MQ=255)
                                                                                                                                                     gTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAgcggc  <  8:190833/145‑3 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GGGCATCACCCTCGCCTCGCTGGGCCTGGGCTGGGTGGGTGAGCCCGCCTTCGCCCACTTGCTGGAACCATTGCTGGC‑CTATC‑TCGGCGTGGACAGCGCCGAGCTGGTCAAGGCGATCTCGTTCTTCGTCGCCTTCTTCGTCATCTCGTACCTGCACATCGTGGTCGGCGAACTGGCCCCCAAGTCCTGGGCTATCCGCAA‑GCCAGAGCTGCTGTCGCTGTGGACCGCCGTGCCGCTTTACCTGTTCTACTGGGCGATGTACCCGGCCATCTACCTGCTCAATGCCAGCGCC  >  NC_002947/6067588‑6067879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑