Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,078,930 C→T 6.1% S99N (AGC→AAC)  PP_5330 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,078,9300CT 6.1% 249.2 / 3.1 83S99N (AGC→AAC) PP_5330MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (46/30);  new base T (3/2);  total (50/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

ACCCATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAA  >  NC_002947/6078786‑6079078
                                                                                                                                                |                                                                                                                                                    
acCCATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAg                                                                                                                                                  >  2:398865/1‑149 (MQ=255)
acCCATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAg                                                                                                                                                  >  8:201842/1‑149 (MQ=255)
acCCATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAg                                                                                                                                                  >  7:322026/1‑149 (MQ=255)
 cccATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAgg                                                                                                                                                 <  3:193486/149‑1 (MQ=255)
 cccATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAgg                                                                                                                                                 <  7:31315/149‑1 (MQ=255)
      gcgcCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGaa                                                                                                                                            >  5:257653/1‑149 (MQ=255)
       cgcCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGaa                                                                                                                                            <  5:298438/148‑1 (MQ=255)
       cgcCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAAc                                                                                                                                           <  1:253447/149‑1 (MQ=255)
           cGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACAtct                                                                                                                                       >  3:58128/1‑149 (MQ=255)
            ggTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACAtct                                                                                                                                       >  6:220499/1‑148 (MQ=255)
                cTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATcttctt                                                                                                                                   <  2:61313/148‑1 (MQ=255)
                 ttCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCATGGTGAACATCTTCATgc                                                                                                                                 >  3:318317/1‑149 (MQ=255)
                   cgccgcCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTgcg                                                                                                                                <  5:296018/148‑1 (MQ=255)
                        ccgATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAg                                                                                                                          >  6:197265/1‑149 (MQ=255)
                                cgcgCCTTGGATCACGCGCATCAGCAACAGCAGGATCTGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGc                                                                                                                  >  1:67364/1‑149 (MQ=255)
                                cgcgCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGc                                                                                                                  <  3:305334/149‑1 (MQ=255)
                                cgcgCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGc                                                                                                                  <  4:268812/149‑1 (MQ=255)
                                 gcgcCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAATTCGcc                                                                                                                 >  4:238660/1‑149 (MQ=255)
                                  cgcCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGGCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCg                                                                                                                <  5:348844/149‑1 (MQ=255)
                                      ttGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGt                                                                                                            >  1:300950/1‑149 (MQ=255)
                                      ttGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGt                                                                                                            >  2:152478/1‑149 (MQ=255)
                                         gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTggg                                                                                                         <  2:300950/149‑1 (MQ=255)
                                         gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTggg                                                                                                         >  7:151537/1‑149 (MQ=255)
                                         gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTgg                                                                                                          <  5:122807/148‑1 (MQ=255)
                                              cgcgCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGCACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCat                                                                                                     >  2:310095/1‑148 (MQ=255)
                                                    tCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGATCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatgatgatg                                                                                              >  8:136056/1‑149 (MQ=255)
                                                           cagcagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCa                                                                                       <  2:160967/149‑1 (MQ=255)
                                                            agcagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAg                                                                                      >  6:49013/1‑149 (MQ=255)
                                                            agcagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAg                                                                                      >  3:380171/1‑149 (MQ=255)
                                                              cagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCg                                                                                    <  1:301526/149‑1 (MQ=255)
                                                              cagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCg                                                                                    <  8:237122/149‑1 (MQ=255)
                                                                   tCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCcgc                                                                               >  7:299253/1‑149 (MQ=255)
                                                                   tCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCcgc                                                                               <  1:398865/149‑1 (MQ=255)
                                                                      gTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCTTGATGATGCCGCCCAGCGGCCgcgc                                                                             >  4:288540/1‑148 (MQ=255)
                                                                      gTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCgcgc                                                                             >  4:128908/1‑148 (MQ=255)
                                                                       tGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGc                                                                                                                  <  5:43183/110‑1 (MQ=255)
                                                                       tGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGc                                                                                                                  >  6:43183/1‑110 (MQ=255)
                                                                       tGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCa                                                                           <  5:49013/149‑1 (MQ=255)
                                                                         cccACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAgg                                                                         >  5:136566/1‑149 (MQ=255)
                                                                          ccACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAgg                                                                         >  7:343269/1‑148 (MQ=255)
                                                                           cacaTGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAgg                                                                         >  6:333593/1‑147 (MQ=255)
                                                                           cacaTGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTa                                                                       <  1:152478/149‑1 (MQ=255)
                                                                                    aTCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatg                                                                                                    <  1:319271/111‑1 (MQ=255)
                                                                                    aTCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatg                                                                                                    >  2:319271/1‑111 (MQ=255)
                                                                                        gcgcATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGgcggcg                                                             >  4:23050/1‑146 (MQ=255)
                                                                                        gcgcATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGgcggcg                                                             >  1:385923/1‑146 (MQ=255)
                                                                                         cgcATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGgcggcg                                                             <  2:67364/145‑1 (MQ=255)
                                                                                          gcATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCAGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGAt                                                        >  1:265712/1‑149 (MQ=255)
                                                                                            aTACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGACGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGc                                                      >  3:99147/1‑149 (MQ=255)
                                                                                              aCGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGcc                                                     >  2:254555/1‑148 (MQ=255)
                                                                                                gTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGaa                                                  >  6:24653/1‑149 (MQ=255)
                                                                                                gTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGaa                                                  >  6:161177/1‑149 (MQ=255)
                                                                                                gTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGaa                                                  >  7:143410/1‑149 (MQ=255)
                                                                                                             ccATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGTCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATAAGc                                     >  7:302837/1‑149 (MQ=255)
                                                                                                             ccATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCTAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGc                                     >  5:59628/1‑149 (MQ=255)
                                                                                                             ccATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGc                                     >  1:99399/1‑149 (MQ=255)
                                                                                                              cATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGcc                                    >  3:227938/1‑149 (MQ=255)
                                                                                                                  aTCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAg                                <  1:254555/149‑1 (MQ=255)
                                                                                                                  aTCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAg                                >  5:215129/1‑149 (MQ=255)
                                                                                                                  aTCAGGGGCGGCAGGGCCATCATGAAGATGCTCAGGGTGAATATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGTCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGTGAAGATGCCGAACGTTTGCATCAGCCGCa                                 <  4:318317/148‑1 (MQ=255)
                                                                                                                       ggTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACt                           >  4:108219/1‑149 (MQ=255)
                                                                                                                       ggTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACt                           >  5:245987/1‑149 (MQ=255)
                                                                                                                       ggTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGAGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCAc                            >  4:279905/1‑148 (MQ=255)
                                                                                                                         tCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCg                         <  5:333593/149‑1 (MQ=255)
                                                                                                                         tCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCg                         <  8:322026/149‑1 (MQ=255)
                                                                                                                          cGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCTTGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCgg                        <  3:288540/149‑1 (MQ=255)
                                                                                                                          cGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCgg                        <  4:380171/149‑1 (MQ=255)
                                                                                                                              aGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCAt                    >  1:385839/1‑149 (MQ=255)
                                                                                                                                ggCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGt                  <  6:215129/149‑1 (MQ=255)
                                                                                                                                ggCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGt                  >  7:228837/1‑149 (MQ=255)
                                                                                                                                 gCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTc                 <  3:128908/149‑1 (MQ=255)
                                                                                                                                    atcatGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGc              >  8:302670/1‑149 (MQ=255)
                                                                                                                                      caagAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCg            >  2:83629/4‑149 (MQ=25)
                                                                                                                                       atGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCgg           >  2:340693/1‑149 (MQ=255)
                                                                                                                                       aagAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCgg           >  5:100833/3‑149 (MQ=25)
                                                                                                                                         gAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGga         <  1:83629/149‑1 (MQ=25)
                                                                                                                                         gAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGACCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCAt                    >  3:334598/1‑138 (MQ=255)
                                                                                                                                         gAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGACCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCAt                    <  4:334598/138‑1 (MQ=255)
                                                                                                                                         gAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCgg                        >  7:314700/1‑134 (MQ=255)
                                                                                                                                         gAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCgg                        <  8:314700/134‑1 (MQ=255)
                                                                                                                                         gAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCggg          >  3:267536/1‑148 (MQ=255)
                                                                                                                                          aaGATGGTCAGTGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGgaa        <  4:58128/149‑1 (MQ=255)
                                                                                                                                           aGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGgaag       <  8:299253/149‑1 (MQ=255)
                                                                                                                                                cTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGGAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaaca   <  8:151537/148‑1 (MQ=255)
                                                                                                                                                cTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaacaa  >  1:93538/1‑149 (MQ=255)
                                                                                                                                                cTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaaca   >  4:136428/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
ACCCATGCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAA  >  NC_002947/6078786‑6079078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑