Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,078,936 G→C 5.7% T97S (ACC→AGC) ‡ PP_5330 ← MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,078,9360GC 5.7% 280.3 / 6.1 87T97S (ACC→AGC) ‡PP_5330MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (49/33);  new base C (3/2);  total (52/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

GCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGCC  >  NC_002947/6078792‑6079084
                                                                                                                                                |                                                                                                                                                    
gcgcCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGaa                                                                                                                                                  >  5:257653/1‑149 (MQ=255)
 cgcCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGaa                                                                                                                                                  <  5:298438/148‑1 (MQ=255)
 cgcCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAAc                                                                                                                                                 <  1:253447/149‑1 (MQ=255)
     cGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACAtct                                                                                                                                             >  3:58128/1‑149 (MQ=255)
      ggTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACAtct                                                                                                                                             >  6:220499/1‑148 (MQ=255)
          cTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATcttctt                                                                                                                                         <  2:61313/148‑1 (MQ=255)
           ttCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCATGGTGAACATCTTCATgc                                                                                                                                       >  3:318317/1‑149 (MQ=255)
             cgccgcCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTgcg                                                                                                                                      <  5:296018/148‑1 (MQ=255)
                  ccgATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAg                                                                                                                                >  6:197265/1‑149 (MQ=255)
                          cgcgCCTTGGATCACGCGCATCAGCAACAGCAGGATCTGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGc                                                                                                                        >  1:67364/1‑149 (MQ=255)
                          cgcgCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGc                                                                                                                        <  3:305334/149‑1 (MQ=255)
                          cgcgCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGc                                                                                                                        <  4:268812/149‑1 (MQ=255)
                           gcgcCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAATTCGcc                                                                                                                       >  4:238660/1‑149 (MQ=255)
                            cgcCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGGCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCg                                                                                                                      <  5:348844/149‑1 (MQ=255)
                                ttGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGt                                                                                                                  >  1:300950/1‑149 (MQ=255)
                                ttGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGt                                                                                                                  >  2:152478/1‑149 (MQ=255)
                                   gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTggg                                                                                                               <  2:300950/149‑1 (MQ=255)
                                   gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTggg                                                                                                               >  7:151537/1‑149 (MQ=255)
                                   gATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTgg                                                                                                                <  5:122807/148‑1 (MQ=255)
                                        cgcgCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGCACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCat                                                                                                           >  2:310095/1‑148 (MQ=255)
                                              tCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGATCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatgatgatg                                                                                                    >  8:136056/1‑149 (MQ=255)
                                                     cagcagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCa                                                                                             <  2:160967/149‑1 (MQ=255)
                                                      agcagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAg                                                                                            >  6:49013/1‑149 (MQ=255)
                                                      agcagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAg                                                                                            >  3:380171/1‑149 (MQ=255)
                                                        cagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCg                                                                                          <  8:237122/149‑1 (MQ=255)
                                                        cagGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCg                                                                                          <  1:301526/149‑1 (MQ=255)
                                                             tCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCcgc                                                                                     <  1:398865/149‑1 (MQ=255)
                                                             tCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCcgc                                                                                     >  7:299253/1‑149 (MQ=255)
                                                                gTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCTTGATGATGCCGCCCAGCGGCCgcgc                                                                                   >  4:288540/1‑148 (MQ=255)
                                                                gTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCgcgc                                                                                   >  4:128908/1‑148 (MQ=255)
                                                                 tGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGc                                                                                                                        <  5:43183/110‑1 (MQ=255)
                                                                 tGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGc                                                                                                                        >  6:43183/1‑110 (MQ=255)
                                                                 tGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCa                                                                                 <  5:49013/149‑1 (MQ=255)
                                                                   cccACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAgg                                                                               >  5:136566/1‑149 (MQ=255)
                                                                    ccACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAgg                                                                               >  7:343269/1‑148 (MQ=255)
                                                                     cacaTGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAgg                                                                               >  6:333593/1‑147 (MQ=255)
                                                                     cacaTGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTa                                                                             <  1:152478/149‑1 (MQ=255)
                                                                              aTCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatg                                                                                                          >  2:319271/1‑111 (MQ=255)
                                                                              aTCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCatg                                                                                                          <  1:319271/111‑1 (MQ=255)
                                                                                  gcgcATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGgcggcg                                                                   >  4:23050/1‑146 (MQ=255)
                                                                                  gcgcATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGgcggcg                                                                   >  1:385923/1‑146 (MQ=255)
                                                                                   cgcATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGgcggcg                                                                   <  2:67364/145‑1 (MQ=255)
                                                                                    gcATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCAGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGAt                                                              >  1:265712/1‑149 (MQ=255)
                                                                                      aTACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGACGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGc                                                            >  3:99147/1‑149 (MQ=255)
                                                                                        aCGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGcc                                                           >  2:254555/1‑148 (MQ=255)
                                                                                          gTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGaa                                                        >  6:24653/1‑149 (MQ=255)
                                                                                          gTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGaa                                                        >  6:161177/1‑149 (MQ=255)
                                                                                          gTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGaa                                                        >  7:143410/1‑149 (MQ=255)
                                                                                                       ccATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGTCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATAAGc                                           >  7:302837/1‑149 (MQ=255)
                                                                                                       ccATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCTAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGc                                           >  5:59628/1‑149 (MQ=255)
                                                                                                       ccATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGc                                           >  1:99399/1‑149 (MQ=255)
                                                                                                        cATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGcc                                          >  3:227938/1‑149 (MQ=255)
                                                                                                            aTCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAg                                      >  5:215129/1‑149 (MQ=255)
                                                                                                            aTCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAg                                      <  1:254555/149‑1 (MQ=255)
                                                                                                            aTCAGGGGCGGCAGGGCCATCATGAAGATGCTCAGGGTGAATATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGTCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGTGAAGATGCCGAACGTTTGCATCAGCCGCa                                       <  4:318317/148‑1 (MQ=255)
                                                                                                                 ggTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACt                                 >  5:245987/1‑149 (MQ=255)
                                                                                                                 ggTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACt                                 >  4:108219/1‑149 (MQ=255)
                                                                                                                 ggTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGAGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCAc                                  >  4:279905/1‑148 (MQ=255)
                                                                                                                   tCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCg                               <  8:322026/149‑1 (MQ=255)
                                                                                                                   tCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCg                               <  5:333593/149‑1 (MQ=255)
                                                                                                                    cGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCTTGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCgg                              <  3:288540/149‑1 (MQ=255)
                                                                                                                    cGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCgg                              <  4:380171/149‑1 (MQ=255)
                                                                                                                        aGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCAt                          >  1:385839/1‑149 (MQ=255)
                                                                                                                          ggCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGt                        <  6:215129/149‑1 (MQ=255)
                                                                                                                          ggCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGt                        >  7:228837/1‑149 (MQ=255)
                                                                                                                           gCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTc                       <  3:128908/149‑1 (MQ=255)
                                                                                                                              atcatGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGc                    >  8:302670/1‑149 (MQ=255)
                                                                                                                                caagAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCg                  >  2:83629/4‑149 (MQ=25)
                                                                                                                                 atGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCgg                 >  2:340693/1‑149 (MQ=255)
                                                                                                                                 aagAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCgg                 >  5:100833/3‑149 (MQ=25)
                                                                                                                                   gAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGga               <  1:83629/149‑1 (MQ=25)
                                                                                                                                   gAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGACCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCAt                          >  3:334598/1‑138 (MQ=255)
                                                                                                                                   gAAGATGTTCACCCTGAGCATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGACCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCAt                          <  4:334598/138‑1 (MQ=255)
                                                                                                                                   gAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCgg                              >  7:314700/1‑134 (MQ=255)
                                                                                                                                   gAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCgg                              <  8:314700/134‑1 (MQ=255)
                                                                                                                                   gAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCggg                >  3:267536/1‑148 (MQ=255)
                                                                                                                                    aaGATGGTCAGTGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGgaa              <  4:58128/149‑1 (MQ=255)
                                                                                                                                     aGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGgaag             <  8:299253/149‑1 (MQ=255)
                                                                                                                                          cTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGGAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaaca         <  8:151537/148‑1 (MQ=255)
                                                                                                                                          cTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaacaa        >  1:93538/1‑149 (MQ=255)
                                                                                                                                          cTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaaca         >  4:136428/1‑148 (MQ=255)
                                                                                                                                           tCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCgg                              >  8:38588/1‑126 (MQ=255)
                                                                                                                                           tCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCgg                              <  7:38588/126‑1 (MQ=255)
                                                                                                                                           tCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaacaac       >  3:231648/1‑149 (MQ=255)
                                                                                                                                           tCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaacaac       >  8:219147/1‑149 (MQ=255)
                                                                                                                                             aGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaacaac       <  1:310095/147‑1 (MQ=255)
                                                                                                                                             aGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGaacaac       <  7:201842/147‑1 (MQ=255)
                                                                                                                                              gggTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTg    <  7:29504/149‑1 (MQ=255)
                                                                                                                                              gggTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTg    <  4:267536/149‑1 (MQ=255)
                                                                                                                                               ggTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGc   <  7:136056/149‑1 (MQ=255)
                                                                                                                                                gTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGcc  <  3:238660/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GCGCCCGGTACTTCGCCGCCGATTGCCGCGCCTTGGATCACGCGCATCAGCAACAGCAGGATCGGTGCCCACATGCCGATCTGCGCATACGTCGGCAGCAGGCCCATGATCAGGGTCGGCAGGGCCATCATGAAGATGCTCAGGGTGAACATCTTCTTGCGCCCGAGCAAGTCGCCGAAGTGGGCCATGATGATGCCGCCCAGCGGCCGCGCCAGGTAGCCGGCGGCGAAGATGCCGAACGTTTGCATCAGCCGCAGCCACTCGGGCATGTCGGCCGGGAAGAACAACTTGCC  >  NC_002947/6078792‑6079084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑