Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,079,628	C→G	6.0%	D14E (GAC→GAG) ‡	PP_5331 →	acyl‑CoA thioesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,079,628	0	C	G	6.0%	266.4 / 5.7	83	D14E (GAC→GAG) ‡	PP_5331	acyl‑CoA thioesterase
Reads supporting (aligned to +/- strand):  ref base C (39/39);  new base G (2/3);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01

GGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACC  >  NC_002947/6079481‑6079776                                                                                                                                                    |                                                                                                                                                     ggCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGATGATCCAATCCCGCAAGGCGAcc                                                                                                                                                     >  4:251203/1‑149 (MQ=255) ggCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGAcc                                                                                                                                                     >  6:120529/1‑149 (MQ=255)     gccCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGc                                                                                                                                                 >  1:325288/1‑149 (MQ=255)      cacAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGccc                                                                                                                                                >  3:268889/3‑149 (MQ=255)       ccAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAACGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCt                                                                                                                                               <  3:228595/149‑1 (MQ=255)       ccAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCCTCGCATGATTGAGCTCGAACAAGAAGATACAATCCCGCAAGGCGACCTGGCCt                                                                                                                                               >  6:309041/1‑149 (MQ=255)          gTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGc                                                                                                                                            <  8:40055/149‑1 (MQ=255)          gTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGc                                                                                                                                            <  7:171757/149‑1 (MQ=255)           tGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCCAGGTCGCCTTGcg                                                                                                                                           >  8:213375/1‑148 (MQ=255)             aaaCTAAATCTGCGTAGTATGTTTCAATCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCaa                                                                                                                                          <  5:368885/148‑1 (MQ=255)             aaaCGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCCAGGTCGCCTTGcgg                                                                                                                                          <  7:213375/148‑3 (MQ=255)                cGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCTGCAAGGCCAGGTCGCCTTGcgggatt                                                                                                                                      <  1:93987/149‑7 (MQ=255)                cGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCCAGGTCGCCTTGcgggatt                                                                                                                                      <  5:202472/149‑7 (MQ=255)                            agtaTGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCcgc                                                                                                                          >  3:5365/1‑149 (MQ=255)                             gtaTGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCcgc                                                                                                                          <  1:410905/148‑1 (MQ=255)                                tGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCgaga                                                                                                                      <  2:227970/149‑1 (MQ=255)                                  tttCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGaaa                                                                                                                    >  2:160596/1‑149 (MQ=255)                                  tttCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCAGCGAGaac                                                                                                                    >  6:288486/1‑148 (MQ=255)                                   ttCAAGCTGAATCGTTTACCCGGCCTTTGCTCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGaaa                                                                                                                    <  5:45057/148‑1 (MQ=255)                                        gCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACg                                                                                                              >  3:79401/1‑149 (MQ=255)                                                gcttccccGGCCTTTGCGCCACACCAACGAACAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCTAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTgg                                                                                                       <  4:268889/143‑1 (MQ=255)                                                gTTTACCCGGCCTTTGCGCCACACCAACGAGAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTgg                                                                                                       <  6:271134/148‑1 (MQ=255)                                                gTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGc                                                                                                      <  7:75396/149‑1 (MQ=255)                                                 tttACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCg                                                                                                     <  1:234602/149‑1 (MQ=255)                                                  ttACCCGGCCTTTGCGCCACAGCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGGTAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCTAGAAACCAACGGGTTTgg                                                                                                       <  8:1842/146‑1 (MQ=255)                                                   tACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGa                                                                                                    >  3:392021/1‑148 (MQ=255)                                                    aCCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGat                                                                                                   <  1:160596/148‑1 (MQ=255)                                                    aCCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGat                                                                                                   <  1:208263/148‑1 (MQ=255)                                                      ccGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAAc                                                                                                                   >  6:136045/1‑130 (MQ=255)                                                      ccGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAAc                                                                                                                   <  5:136045/130‑1 (MQ=255)                                                      ccGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATAtt                                                                                                >  5:17183/1‑149 (MQ=255)                                                      ccGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATAtt                                                                                                >  4:128240/1‑149 (MQ=255)                                                       cGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCTGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCaa                                                                                                                <  4:224738/132‑1 (MQ=255)                                                       cGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCaa                                                                                                                >  3:224738/1‑132 (MQ=255)                                                         gCCTTTCCCCAACACAAACCAAATCGGTCAGGGGCCAGAGGCCCAATCCCATATTTGAGCTCCAACATGATTATCCAATCCGGAACGGCCACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTc                                                                                             <  1:336509/149‑1 (MQ=255)                                                              tGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTcggcg                                                                                         <  1:381820/148‑1 (MQ=255)                                                              tGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTcggcg                                                                                         <  4:392021/148‑1 (MQ=255)                                                                cgcCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGtt                                                                                      <  1:61675/149‑1 (MQ=255)                                                                cgcCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGtt                                                                                      <  8:213193/149‑1 (MQ=255)                                                                cgcCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGt                                                                                       <  6:198117/148‑1 (MQ=255)                                                                 gcCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAACTCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTg                                                                                     >  4:289858/1‑149 (MQ=255)                                                                 gcCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTg                                                                                     >  6:344852/1‑149 (MQ=255)                                                                   cacaCCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGc                                                                                   >  8:178152/1‑149 (MQ=255)                                                                          aCGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTgc                                                                            >  8:138867/1‑149 (MQ=255)                                                                          aCGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTgc                                                                            >  7:190184/1‑149 (MQ=255)                                                                               aaGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTgcg                                                                           >  2:432327/1‑145 (MQ=255)                                                                                      tcGGGTGAGAGGCCGCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGC‑AGGCGACCTGTCCTTGCAAATCACCGAATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTgcgc                                                                          <  8:315983/137‑1 (MQ=255)                                                                                      aCGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTcgg                                                                                           >  1:298094/1‑122 (MQ=255)                                                                                      aCGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTcgg                                                                                           <  2:298094/122‑1 (MQ=255)                                                                                      aCGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGC‑AGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTgcga                                                                          >  7:315983/1‑137 (MQ=255)                                                                                        gggTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGTGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGTGCAGATGGACCTgg                                                              >  4:202843/1‑149 (MQ=255)                                                                                        gggTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTgg                                                              >  7:160016/1‑149 (MQ=255)                                                                                         ggTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTggc                                                             <  2:157421/149‑1 (MQ=255)                                                                                              gagGCCCCATCGCATGAATGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGGTGGTTGCGAAGATGGACCTggcaggca                                                        >  8:406139/1‑149 (MQ=255)                                                                                                    ccATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCa                                                  >  1:345371/1‑149 (MQ=255)                                                                                                     cATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCAt                                                 >  7:20507/1‑149 (MQ=255)                                                                                                      aTCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATg                                                <  2:325288/149‑1 (MQ=255)                                                                                                      aTCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATg                                                >  4:140580/1‑149 (MQ=255)                                                                                                         gCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGc                                              >  5:272511/1‑148 (MQ=255)                                                                                                               ttGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCgt                                       >  1:436035/1‑149 (MQ=255)                                                                                                                  aGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTc                                    <  6:17183/149‑1 (MQ=255)                                                                                                                    cTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCATCGGGTTTGGCGATATTTTCGGCGGTTGGCTGGGTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGc                                  <  5:309041/149‑1 (MQ=255)                                                                                                                    cTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGc                                  <  2:345371/149‑1 (MQ=255)                                                                                                                     tCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCgt                                       <  3:284414/143‑1 (MQ=255)                                                                                                                     tCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCgt                                       >  4:284414/1‑143 (MQ=255)                                                                                                                     tCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGcc                                 >  5:1671/1‑149 (MQ=255)                                                                                                                       gAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCcgg                               >  6:171199/1‑149 (MQ=255)                                                                                                                        aaCAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCcggc                              <  7:138867/149‑1 (MQ=255)                                                                                                                         aCAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCcggcg                             >  2:131761/1‑149 (MQ=255)                                                                                                                         aCAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCcggcg                             <  4:5365/149‑1 (MQ=255)                                                                                                                          cAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCcggcgg                            <  7:200642/149‑1 (MQ=255)                                                                                                                             gaagaTCCAATCCCGCAAGTCGACCTGGCCTTGAAAATCACCGCATTGCCGCGAGAAACCAACGGTTTTTGCGATATTTTCGGCGGTATCCTGGTTGCGCAGAAGGACCTGGCAGGCACCGCCATGGCCAGCCGTGTCTCCGGCGGCcg                         >  5:346932/1‑149 (MQ=255)                                                                                                                              aagaTCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCcgc                        <  3:77494/149‑1 (MQ=255)                                                                                                                                   ccAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGc                   >  8:339939/1‑149 (MQ=255)                                                                                                                                     aaTCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCa                 >  5:405709/1‑149 (MQ=255)                                                                                                                                     aaTCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCa                 >  4:411667/1‑149 (MQ=255)                                                                                                                                     aaTCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCa                 >  2:287556/1‑149 (MQ=255)                                                                                                                                     aaTCCCGCAACGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCa                 <  5:288486/149‑1 (MQ=255)                                                                                                                                      aTCCCGCAAGGTGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGTGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCAc                <  3:202843/149‑1 (MQ=255)                                                                                                                                        tttgCAAGGCCAGGTCGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCg              >  1:257677/4‑149 (MQ=255)                                                                                                                                          cGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTc            >  8:156476/1‑149 (MQ=255)                                                                                                                                           ggaaGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCg           <  8:20507/147‑1 (MQ=255)                                                                                                                                           gCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCg           >  7:374326/1‑149 (MQ=255)                                                                                                                                             cctgggcccTGGCCTAGCAAATCACCGCATTGCCGCGAGCAACCAACGGGGTTGGCGATCTTTTCGGCGGTTGGCGGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGcc         <  8:355171/142‑1 (MQ=255)                                                                                                                                                gCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATc      <  3:251203/149‑1 (MQ=255)                                                                                                                                                  gACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGa    <  2:436035/149‑1 (MQ=255)                                                                                                                                                   tccTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGTCACGGCCATGGCCAGCCGTGTCGACGGCGGCCGCGTAGCCACCGTCGCCATCGAc   <  6:346932/148‑1 (MQ=255)                                                                                                                                                    ccTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGAcc  >  5:287145/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATCGCATGATTGAGCTCGAACAAGAAGATCCAATCCCGCAAGGCGACCTGGCCTTGCAAATCACCGCATTGCCGCGAGAAACCAACGGGTTTGGCGATATTTTCGGCGGTTGGCTGGTTGCGCAGATGGACCTGGCAGGCACGGCCATGGCCAGCCGTGTCGCCGGCGGCCGCGTAGCCACCGTCGCCATCGACC  >  NC_002947/6079481‑6079776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑