Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,108,335	G→C	5.6%	W23C (TGG→TGC)	PP_5358 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,108,335	0	G	C	5.6%	289.4 / 6.4	90	W23C (TGG→TGC)	PP_5358	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (46/38);  new base C (2/3);  total (48/42)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

CGCAGCGCAGTATGAACTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGCCGCAAGCGCCTGGCAC  >  NC_002947/6108187‑6108477                                                                                                                                                      |                                                                                                                                                 cgcagcgcagTATGAACTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTgg                                                                                                                                                  <  7:118357/149‑1 (MQ=255)  gcagcgcagTATGAACTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTgg                                                                                                                                                  >  7:89045/1‑148 (MQ=255)   cagcgcagTATGAACTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGca                                                                                                                                                <  6:9309/149‑1 (MQ=255)      cgcagTATGAACTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACaa                                                                                                                                             >  3:315941/1‑149 (MQ=255)      cgcagTATGAACTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACaa                                                                                                                                             >  5:415264/1‑149 (MQ=255)       gcagTATGAACTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAAc                                                                                                                                            >  2:179471/1‑149 (MQ=255)          gTATGAACTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCAt                                                                                                                                         <  2:30486/149‑1 (MQ=255)          gTATGAACTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGCCACAACCag                                                                                                                                         <  3:227866/149‑2 (MQ=255)          gTATGAACTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTCACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGCCACAACCag                                                                                                                                         <  3:202476/149‑2 (MQ=255)           tATGAACTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAGTGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCAGGTTGTGGCACAACCATg                                                                                                                                        >  2:85050/1‑149 (MQ=255)               aaCTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGtt                                                                                                                                    >  7:80322/1‑149 (MQ=255)                aCTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTg                                                                                                                                   >  8:386500/1‑149 (MQ=255)                    gATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCg                                                                                                                                 <  5:421127/147‑1 (MQ=255)                    gATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGt                                                                                                                                <  7:320267/148‑1 (MQ=255)                        gCTACCTTTACGAAAGGCCAGGAACGCAGTTAAGGTGGATCTACCTTTTTGGAGCATATGCAAATGTTTTCCCTGGAGAACCTCTTCGTCCCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGaa                                                                                                                             <  1:125348/148‑1 (MQ=255)                        gCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGaa                                                                                                                             >  2:385562/1‑147 (MQ=255)                            ccTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAgg                                                                                                                         <  4:302920/147‑1 (MQ=255)                             cTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGccc                                                                                                                      <  2:268953/149‑1 (MQ=255)                               gTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTg                                                                                                                    >  6:116692/1‑149 (MQ=255)                                tACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCt                                                                                                                     <  5:410849/147‑1 (MQ=255)                                tACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTg                                                                                                                    <  4:315941/148‑1 (MQ=255)                                     cAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACgg                                                                                                             <  4:402638/149‑1 (MQ=255)                                      aGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGaa                                                                                                                             <  7:381845/133‑1 (MQ=255)                                      aGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGaa                                                                                                                             >  8:381845/1‑133 (MQ=255)                                       ggCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGACACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGT‑AACGGGt                                                                                                           <  8:89045/149‑1 (MQ=255)                                        gCCAGGACCGCAGCTAAGCCGTATCTACCTGTTTGGAGCACATGCAAAGGTTGACCCTGGACAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTc                                                                                                          <  4:394000/149‑1 (MQ=255)                                        gCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGt                                                                                                           >  1:208701/1‑148 (MQ=255)                                        gCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGt                                                                                                           >  1:143684/1‑148 (MQ=255)                                         ccAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAGTGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCAGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCa                                                                                                         <  1:85050/149‑1 (MQ=255)                                         ccAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCAACGGCAGGCGCCTGGTTGTGGCACAACCAGGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTc                                                                                                          >  5:92522/1‑148 (MQ=255)                                          cAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCa                                                                                                         >  6:311826/1‑148 (MQ=255)                                          cAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCa                                                                                                         >  1:276839/1‑148 (MQ=255)                                           aGGTACGCATTTAAGCTGTATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTGTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCaaa                                                                                                       <  8:80322/149‑1 (MQ=255)                                                cGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCa                                                                                                  >  2:117864/1‑149 (MQ=255)                                                 gCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCAt                                                                                                 >  2:289685/1‑149 (MQ=255)                                                   aGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTg                                                                                               >  3:191485/1‑149 (MQ=255)                                                   aGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAAACATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTg                                                                                               >  4:350906/1‑149 (MQ=255)                                                        aGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACATCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCAAGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGAATTTCGcc                                                                                           >  7:60033/1‑148 (MQ=255)                                                            ggATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACt                                                                                      >  4:293760/1‑149 (MQ=255)                                                                  aCCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCt                                                                                >  1:104561/1‑149 (MQ=255)                                                                  aCCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑ACTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCt                                                                                >  1:315687/1‑149 (MQ=255)                                                                   ccTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGAc                                                                                  <  2:104039/146‑1 (MQ=255)                                                                    cTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTg                                                                               >  5:361102/1‑148 (MQ=255)                                                                     tGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTg                                                                               >  7:254776/1‑147 (MQ=255)                                                                          ggAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAACCTCGACCTGGAGTTGc                                                                        >  3:342826/1‑149 (MQ=255)                                                                           gAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCt                                                                       >  6:49635/1‑149 (MQ=255)                                                                             gCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGACTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTg                                                                     <  6:418890/149‑1 (MQ=255)                                                                              cATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGa                                                                    <  1:179471/149‑1 (MQ=255)                                                                               atatGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGAc                                                                   <  3:293760/149‑1 (MQ=255)                                                                                   gCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGGCAACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGa                                                                    <  1:96878/145‑1 (MQ=255)                                                                                   gCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGa                                                                    >  2:96878/1‑144 (MQ=255)                                                                                   gCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATg                                                               >  3:78221/1‑149 (MQ=255)                                                                                   gCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATg                                                               >  4:82031/1‑149 (MQ=255)                                                                                          ttGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑gc                                                       <  4:191485/149‑1 (MQ=255)                                                                                               ccTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTc                                                   >  2:254536/1‑148 (MQ=255)                                                                                               ccTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGACACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCACTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTc                                                   >  5:218592/1‑148 (MQ=255)                                                                                                cTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTc                                                   >  4:325659/1‑147 (MQ=255)                                                                                                        ccTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGTATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCg                                         >  2:18404/1‑149 (MQ=255)                                                                                                        ccTCTTCGT‑CCTGATGCTGGTAGCCACGCCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCg                                         >  1:247670/1‑149 (MQ=255)                                                                                                         ctctTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGTGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGc                                        >  2:102309/1‑149 (MQ=255)                                                                                                         ctctTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGc                                        >  7:197080/1‑149 (MQ=255)                                                                                                           ctTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCt                                      >  2:319130/1‑149 (MQ=255)                                                                                                           catctt‑CCTGACGCTGTTAGCCACGGCATGCGCCTGGTTGGCGCACAACCATGGGTTGCGTGACAAGGCCCTGG‑ACCGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCt                                      <  7:368148/144‑1 (MQ=255)                                                                                                            ttCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGc                                               <  4:49778/139‑1 (MQ=255)                                                                                                            ttCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGc                                               >  3:49778/1‑139 (MQ=255)                                                                                                             tttgttcTGTTGCTGGTTTCCAAGGCAGGCGCCTGGTTGTTTCACAACCATGGGTTGGGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCt                                      <  8:60033/142‑1 (MQ=255)                                                                                                              cGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCtt                                     <  5:116692/147‑1 (MQ=255)                                                                                                              cGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCtt                                     <  2:208701/147‑1 (MQ=255)                                                                                                              cGT‑CCTGATGCTGGTAGCCACGGCAGGCCCCTGGTTGTGGCACAACCATGAGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAAATCGACCTGGAGTTGCTTGACGATGACGTA‑GCGCTCAAGCGTATCGCCTTTg                                   >  4:178138/1‑149 (MQ=255)                                                                                                                t‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTc                                 <  3:325659/149‑1 (MQ=255)                                                                                                                   cTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCg                               <  7:386500/149‑1 (MQ=255)                                                                                                                      aTGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCgg                              <  8:197080/147‑1 (MQ=255)                                                                                                                        gCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGtttgacag                           >  7:79324/1‑141 (MQ=255)                                                                                                                         cTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATg                          <  6:361102/148‑1 (MQ=255)                                                                                                                          tGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCggg                             <  2:376633/144‑1 (MQ=255)                                                                                                                          tGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCggg                             >  1:376633/1‑144 (MQ=255)                                                                                                                             tAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAAt                     <  1:385562/149‑1 (MQ=255)                                                                                                                              aGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGCGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGCAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTTTCCGGGATGCCAATg                    >  6:274847/1‑149 (MQ=255)                                                                                                                               gCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATg                    <  1:254536/148‑1 (MQ=255)                                                                                                                                ccACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGc                  >  3:378021/1‑149 (MQ=255)                                                                                                                                ccACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGc                  <  1:319130/149‑1 (MQ=255)                                                                                                                                 cACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGcc                 >  5:402057/1‑149 (MQ=255)                                                                                                                                   cGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGCCGc               <  2:104561/149‑1 (MQ=255)                                                                                                                                   cGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGCCGc               <  4:78221/149‑1 (MQ=255)                                                                                                                                         gcgcCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGCCGCAAgcgc         <  3:350906/149‑1 (MQ=255)                                                                                                                                           gcCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCTAGCGTATCGCCTTTGTCCGGGATGCCAATGGCCGCAAGCGCCt       <  1:117864/149‑1 (MQ=255)                                                                                                                                           ccatGGTTGTGCCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGCCGCAAGCGCCt       >  7:357844/4‑149 (MQ=255)                                                                                                                                            catGGTTGTGCCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGCCGCAAGCGCCTg      >  5:5710/3‑149 (MQ=255)                                                                                                                                             cTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGCCGCAAGCGCCTgg     >  2:295372/1‑149 (MQ=255)                                                                                                                                             atGGTTGTGCCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGCCGCAAGCGCCTgg     <  6:5710/148‑1 (MQ=255)                                                                                                                                                gTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGCCGCAAGCGCCTGGCAc  >  7:311183/1‑149 (MQ=255)                                                                                                                                                 ttGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTATGCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGCCGCAAGCGCCTGGCAc  <  1:120023/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                 CGCAGCGCAGTATGAACTTGATTGCTACCTGTACGACAGGCCAGGAACGCAGTTAAGCTGGATCTACCTGTTTGGAGCATATGCAAATGTTGACCCTGGAGAACCTCTTCGT‑CCTGATGCTGGTAGCCACGGCAGGCGCCTGGTTGTGGCACAACCATGGGTTGCGTGAAAAGGCCCTGG‑AACGGGTCAAACAGCATTGCGCCAAACTCGACCTGGAGTTGCTTGACGATGCCGTA‑GCGCTCAAGCGTATCGCCTTTGTCCGGGATGCCAATGGCCGCAAGCGCCTGGCAC  >  NC_002947/6108187‑6108477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑