BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,111,977	T→A	8.7%	H118Q (CAT→CAA)	PP_5362 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,111,977	0	T	A	8.7%	294.5 / 14.6	103	H118Q (CAT→CAA)	PP_5362	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (49/45); new base A (2/7); total (51/52)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.56e-01

Only 100 of 105 total aligned reads displayed.

GGGCACAAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTATGCCGTAAC  >  NC_002947/6111829‑6112124

gggCACAAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCAt                                                                                                                                                     >  6:48169/1‑149 (MQ=255)

cccgtcaAACCAACAGCCGCTATGTCACATTGAATTTTTTTACCGGCCAGCCCAGCTATGGTGAGGCCTGGGTGGTCTACAGCCG‑‑CCTCTTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGTGAGCGCTTCAAGCTGCAt                                                                                                                                                     <  5:142814/144‑1 (MQ=255)

  gCACAAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTg                                                                                                                                                   <  4:359456/149‑1 (MQ=255)

  gCACAAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTg                                                                                                                                                   >  5:222639/1‑149 (MQ=255)

    acaAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTg                                                                                                                                                   >  1:191310/1‑147 (MQ=255)

    acaAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTg                                                                                                                                                   >  1:197703/1‑147 (MQ=255)

      aaaCCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGc                                                                                                                                               >  2:259587/1‑149 (MQ=255)

      aaaCCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGc                                                                                                                                               >  5:416953/1‑149 (MQ=255)

       aaCCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCt                                                                                                                                              <  8:8157/149‑1 (MQ=255)

       aaCCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCt                                                                                                                                              >  8:204683/1‑149 (MQ=255)

        aCCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCtt                                                                                                                                             >  6:209334/1‑149 (MQ=255)

           aaCAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGTGCTACAGCCG‑‑CCGCGTATGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCAATGCAGCTTGaa                                                                                                                                          <  8:139843/149‑2 (MQ=255)

           aaCAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCAATGCAGCTTGaa                                                                                                                                          <  5:352511/149‑2 (MQ=255)

           aaCAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCAATGCAGCTTGaa                                                                                                                                          <  5:180943/149‑2 (MQ=255)

           aaCAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCAATGCAGCTTGaa                                                                                                                                          <  1:332850/149‑2 (MQ=255)

           aaCAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCAATGCAGCTTGaa                                                                                                                                          <  3:265686/149‑2 (MQ=255)

            aCAGCCACTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATc                                                                                                                                         >  2:266115/1‑149 (MQ=255)

              aGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATc                                                                                                                                         >  5:138548/1‑147 (MQ=255)

               gCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAg                                                                                                                                      <  6:409757/149‑1 (MQ=255)

                ccGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCCAAGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAg                                                                                                                                      <  1:90440/149‑1 (MQ=255)

                 cGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAggg                                                                                                                                    <  5:242988/149‑1 (MQ=255)

                   cTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCGAAGCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAggg                                                                                                                                    >  1:130281/1‑149 (MQ=255)

                        gCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAgagatgg                                                                                                                                >  7:236279/1‑140 (MQ=255)

                         cacaTTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGtt                                                                                                                            <  7:138885/149‑1 (MQ=255)

                           caTTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGa                                                                                                                          <  1:266115/149‑1 (MQ=255)

                            aTTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGAc                                                                                                                         >  6:199725/1‑149 (MQ=255)

                              tGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAgg                                                                                                                                     <  4:93720/135‑1 (MQ=255)

                              tGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAgg                                                                                                                                     >  3:93720/1‑135 (MQ=255)

                                 cTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGcctc                                                                                                                    <  8:344754/149‑1 (MQ=255)

                                  tttttttACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGcctcc                                                                                                                   >  2:80078/1‑149 (MQ=255)

                                    tttttACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGcctcc                                                                                                                   <  8:291812/147‑1 (MQ=255)

                                     ttttACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCtt                                                                                                                 >  2:111068/1‑148 (MQ=255)

                                          ccGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCAttt                                                                                                           <  1:232282/149‑1 (MQ=255)

                                           cGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGcctc                                                                                                                    >  1:203327/1‑139 (MQ=255)

                                           cGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGcctc                                                                                                                    <  2:203327/139‑1 (MQ=255)

                                            ggCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGGGCCGTTGACGCCTCCTTTCATTTTg                                                                                                         <  7:204683/149‑1 (MQ=255)

                                             gCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGa                                                                                                        <  7:132279/149‑1 (MQ=255)

                                              ccTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGaa                                                                                                       <  5:209334/149‑1 (MQ=255)

                                                  gCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGtt                                                                                                   <  5:48169/149‑1 (MQ=255)

                                                    cAGAGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAAACGCGTATAA‑AGTTCCAGCGTGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGATGGGTGACGTTGACGGCTACTTTAATATTGAActattt                                                                                                 >  2:421069/1‑144 (MQ=255)

                                                        gATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTtct                                                                                             <  3:411878/149‑1 (MQ=255)

                                                           ggTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTctct                                                                                           <  2:197703/148‑1 (MQ=255)

                                                           ggTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTAAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTctct                                                                                           <  2:191310/148‑1 (MQ=255)

                                                             tGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTctgct                                                                                        <  6:416953/149‑1 (MQ=255)

                                                                 gCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGtttttt                                                                                               >  6:91329/1‑138 (MQ=255)

                                                                 gCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGtttttt                                                                                               <  5:91329/138‑1 (MQ=255)

                                                                      ggtgggCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTAtttt                                                                               <  5:199725/149‑1 (MQ=255)

                                                                      ggtgggCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCACTTTGAACGTTTTTTCTCTGCTGCCTAtttt                                                                               <  1:259587/149‑1 (MQ=255)

                                                                          ggCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGt                                                                             >  1:316729/1‑147 (MQ=255)

                                                                          ggCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTaa                                                                           <  4:98183/149‑1 (MQ=255)

                                                                            cTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTctgctg                                                                                       <  4:146691/135‑1 (MQ=255)

                                                                            cTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGt                                                                         >  1:133075/1‑149 (MQ=255)

                                                                            cTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCAGTTTGAACGTTTTTTCTctgctg                                                                                       >  3:146691/1‑135 (MQ=255)

                                                                              aCAGCCG‑‑CCGCGTAGGCTGGACCACATTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCATATTTTGTAAGTCa                                                                       >  1:287488/1‑149 (MQ=255)

                                                                               cAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAg                                                                      >  1:177549/1‑149 (MQ=255)

                                                                                   cg‑‑ccgcGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCAATGCAGCTTGaa                                                                                                                                          <  4:136750/77‑2 (MQ=255)

                                                                                   cg‑‑ccgcGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCAATGCAGCTTGaa                                                                                                                                          >  3:136750/1‑76 (MQ=255)

                                                                                       ccgcGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGa                                                                <  1:80078/149‑1 (MQ=255)

                                                                                             aGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCAcgc                                                          >  2:394324/1‑149 (MQ=255)

                                                                                                   gACCACCTTGAACCGCGTATCC‑AGTTCCATCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGa                                                     >  5:353416/1‑148 (MQ=255)

                                                                                                   gACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGGGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGaa                                                    <  8:385006/149‑1 (MQ=255)

                                                                                                     ccaccTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGaaaa                                                  >  2:71971/1‑149 (MQ=255)

                                                                                                     ccaccTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGaaaa                                                  <  4:244667/149‑1 (MQ=255)

                                                                                                     ccaccTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTGCATTTTGATCGTTTTTTCTCTGCTGCGTATTTTGTAAGTCTGCTTCGAGCCCGCGGTGaata                                                  >  8:410076/1‑147 (MQ=255)

                                                                                                      caccTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGaaaa                                                  >  2:313369/1‑148 (MQ=255)

                                                                                                      caccTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGaaaa                                                  <  4:4210/148‑1 (MQ=255)

                                                                                                        ccTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAacac                                               >  6:175904/1‑149 (MQ=255)

                                                                                                            gAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGGTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGc                                           >  3:191253/1‑149 (MQ=255)

                                                                                                              acagcgTATCC‑AGTTCCAGCGGGGAGAGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCCGCTGCATATTTTGTAAGTCAGCTTCGAGCACGCGCTCAAACCACCAGCCt                                         >  2:134932/4‑149 (MQ=255)

                                                                                                              aCCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCCGCTGCATATTTTGTAAGTCAGCTTCGAGCACGCGCTGACACCACCAGCCt                                         >  2:129494/1‑149 (MQ=255)

                                                                                                               ccGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGt                                                                             <  5:102301/112‑1 (MQ=255)

                                                                                                               ccGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGt                                                                             >  6:102301/1‑112 (MQ=255)

                                                                                                               ccGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCtt                                        >  5:404262/1‑149 (MQ=255)

                                                                                                                cgcgTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTg                                       <  1:313369/149‑1 (MQ=255)

                                                                                                                 gcgTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGCCGCCTCCTTTCATTTTGAACGGTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCATGa                                      >  2:158651/1‑149 (MQ=255)

                                                                                                                   gTATAC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGATCACGCGCTGAAAACACCAGCCTTGAc                                     >  2:259875/1‑148 (MQ=255)

                                                                                                                      tCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAAc                                 <  4:417576/149‑1 (MQ=255)

                                                                                                                       cc‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAAcc                                >  8:329035/1‑149 (MQ=255)

                                                                                                                        c‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAAccc                               >  4:71603/1‑149 (MQ=255)

                                                                                                                          aGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGATGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAAGCCt                              >  4:42487/1‑149 (MQ=255)

                                                                                                                            ttCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCttt                            <  6:165650/149‑1 (MQ=255)

                                                                                                                             tCCAGCGGGGAGGGCTTCAATCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCttt                            <  3:51390/148‑1 (MQ=255)

                                                                                                                               cAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGt                          <  6:138548/148‑1 (MQ=255)

                                                                                                                               cAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGt                          >  3:429648/1‑148 (MQ=255)

                                                                                                                                aGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGt                          >  4:218508/1‑147 (MQ=255)

                                                                                                                                aGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCa                        <  3:68272/149‑1 (MQ=255)

                                                                                                                                 gCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGt                          >  6:284025/1‑146 (MQ=255)

                                                                                                                                  cGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGa                      >  6:386527/1‑149 (MQ=255)

                                                                                                                                       atcgcTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCTTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGaa                 <  1:421069/147‑1 (MQ=255)

                                                                                                                                        gcgcTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGaaa                <  5:175904/149‑1 (MQ=255)

                                                                                                                                         cgcTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGaaaa               <  7:329035/149‑1 (MQ=255)

                                                                                                                                             tCAAGCTGCAATGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGa                                      >  6:124289/1‑122 (MQ=255)

                                                                                                                                             tCAAGCTGCAATGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGa                                      <  5:124289/122‑1 (MQ=255)

                                                                                                                                              cAAGCTGCATTGCAGCTTGATCGAGGGTTTAATTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCCGCTGCCTATTTTGTAAGTCAGCTTCGAGCACTCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCt            <  1:134932/147‑1 (MQ=255)

                                                                                                                                              cAAGCTGCATTGCAGCTTGATCGAGGGGTTCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCCGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCt            <  1:129494/147‑1 (MQ=255)

                                                                                                                                              cAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTa           <  5:386527/148‑1 (MQ=255)

                                                                                                                                                  cTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACTAGCCTTGACCAACCCTTTGTCAGCCCGAAAAGCTATGCCg      >  8:239639/1‑149 (MQ=255)

                                                                                                                                                   tGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTATGCCg      >  4:260956/1‑148 (MQ=255)

                                                                                                                                                   tGCATTGCAGCTTGATCGAGGGGTGCCATTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTATGCCg      >  3:315992/1‑148 (MQ=255)

                                                                                                                                                      aTTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTATGCCGTAAc  >  2:417682/1‑149 (MQ=255)

GGGCACAAACCAACAGCCGCTATGGCACATTGACTTTTTTTACCGGCCTGGCCAGCGATGGTGAGGCCTGGGTGGGCTACAGCCG‑‑CCGCGTAGGCTGGACCACCTTGAACCGCGTATCC‑AGTTCCAGCGGGGAGCGCTTCAAGCTGCATTGCAGCTTGATCGAGGGGTGCCGTTGACGCCTCCTTTCATTTTGAACGTTTTTTCTCTGCTGCCTATTTTGTAAGTCAGCTTCGAGCACGCGCTGAAAACACCAGCCTTGACCAACCCTTTGTCAGACCGAAAAGCTATGCCGTAAC  >  NC_002947/6111829‑6112124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`