| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 583,719 | C→G | 8.7% | P529A (CCC→GCC) ‡ | selB → | selenocysteyl‑tRNA‑specific translation elongation factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 583,719 | 0 | C | G | 8.7% | 271.5 / 14.1 | 92 | P529A (CCC→GCC) ‡ | selB | selenocysteyl‑tRNA‑specific translation elongation factor |
| Reads supporting (aligned to +/- strand): ref base C (43/40); new base G (6/2); total (50/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01 | |||||||||||
TGCTGGATGAACTGCTCGACGACAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCAGCGCATGGCAGCG > NC_002947/583573‑583865 | tGCTGGATGAACTGCTCGACGACAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGaaccaccc > 1:370172/1‑149 (MQ=255) tGGATGAACTGCTCGACGACAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTgg < 7:181913/149‑1 (MQ=255) tGGATGAACTGCTCGACGACAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTgg > 6:146503/1‑149 (MQ=255) gATGAACTGCTCGACGACAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGt > 3:412908/1‑149 (MQ=255) aTGAACTGCTCGACGACAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGt > 5:389759/1‑148 (MQ=255) tGAACTGCTCGACGACAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCAGGGTGGGTCa < 8:43557/149‑1 (MQ=255) cTGCTCGACGACAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACGCAGGGTGGGTCaaat < 4:199772/148‑4 (MQ=255) cTCGACGACAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCt > 7:239765/1‑149 (MQ=255) acgacAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGc > 1:155628/1‑148 (MQ=255) acgacAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGc > 3:351177/1‑148 (MQ=255) acAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGt < 4:412908/149‑1 (MQ=255) cAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTg < 1:12919/149‑1 (MQ=255) aGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGt > 4:317514/1‑147 (MQ=255) gcgcCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTTCAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAACCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTgag < 2:56039/149‑1 (MQ=255) gcgcCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTgag < 1:205301/149‑1 (MQ=255) agcCATGGCCCGCTGCTGGCGATGGCTGCATATGCCCGATCACAAGGTACAGTTGAGCGAAGACGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATAGCAGGGCAATTTGACCCACCCGGGGTCAGGCCGCTGTCGAGTgagg > 6:281126/2‑149 (MQ=255) aTCGACAGCAGCGGGACATGGATGCATCTTACAGATCACAAGGTACAGTTGAGCGAAGCCTACAGTGCATTGTCGGCACGTATGAAGCCGAAGTTGATCGCAGTGCAATTTGACACACACTGGGTCATGACGATGGCGAGTGAGgaga > 4:417964/1‑148 (MQ=255) ccAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACtgt < 7:346608/149‑1 (MQ=255) ccAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCACCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACtgt < 3:184044/149‑1 (MQ=255) cagcagCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACtgtg < 2:387687/149‑1 (MQ=255) cGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAg > 3:359912/1‑149 (MQ=255) cGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAg < 1:79086/149‑1 (MQ=255) cGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAg < 3:154620/149‑1 (MQ=255) gggCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGc < 8:241655/149‑1 (MQ=255) gCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGcc > 2:435319/1‑148 (MQ=255) gCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCg < 5:324544/149‑1 (MQ=255) ccATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGa > 1:177441/1‑149 (MQ=255) cATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGAt < 4:276419/149‑1 (MQ=255) ggCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGa < 8:279016/145‑1 (MQ=255) ggCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGa > 7:279016/1‑145 (MQ=255) gCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTgc < 1:435319/149‑1 (MQ=255) cATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGAtgt > 7:373235/1‑143 (MQ=255) cATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGAtgt < 8:373235/143‑1 (MQ=255) cATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTgcgc < 8:52752/147‑1 (MQ=255) cATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGcc < 3:317514/148‑1 (MQ=255) gCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTAc > 8:245682/1‑149 (MQ=255) cccGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACt > 2:43829/1‑149 (MQ=255) cccGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACt < 2:278189/149‑1 (MQ=255) ccGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGTGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTg > 7:220864/1‑149 (MQ=255) ccGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTg > 4:143732/1‑149 (MQ=255) ccGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTg < 6:11821/149‑1 (MQ=255) tCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTgcgc < 4:359912/148‑1 (MQ=255) ggTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTgg < 7:245682/149‑1 (MQ=255) gTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGc < 1:43829/149‑1 (MQ=255) gTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGc > 2:151779/1‑149 (MQ=255) tGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCt < 2:370172/148‑1 (MQ=255) aGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTgg < 2:155628/148‑1 (MQ=255) cGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGcc > 8:57026/1‑149 (MQ=255) gAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCt > 4:56786/1‑149 (MQ=255) gAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCt > 3:77317/1‑149 (MQ=255) aaGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCTGAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTg > 1:309200/1‑149 (MQ=255) aGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTgg < 3:51711/149‑1 (MQ=255) gCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGt < 8:239765/149‑1 (MQ=255) cGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGc < 2:23568/149‑1 (MQ=255) gACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCa < 4:351177/149‑1 (MQ=255) aCAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCAt < 1:169497/149‑1 (MQ=255) cAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCAt > 6:165516/1‑148 (MQ=255) tGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAgg > 2:108422/1‑149 (MQ=255) cATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAggtg < 3:10590/149‑1 (MQ=255) cATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAggtg < 1:264752/149‑1 (MQ=255) cATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAggt < 5:146503/148‑1 (MQ=255) aTTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAggtgg > 8:182794/1‑149 (MQ=255) gggCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTgcgc > 5:83279/1‑149 (MQ=255) ggCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTgcgc > 5:266941/1‑148 (MQ=255) aCGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACt > 2:198684/1‑149 (MQ=255) cTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGt > 3:122834/1‑148 (MQ=255) tGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTc < 4:122834/149‑1 (MQ=255) gCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTc > 3:218828/1‑148 (MQ=255) aGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTAc < 6:389759/149‑1 (MQ=255) gCCGAAGTTGATCGCAGGGCAATTTGACCCATCCTGGGTCAGGCCGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTAcc > 5:6821/1‑149 (MQ=255) ccGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTAccc > 3:155248/1‑149 (MQ=255) aGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAg < 7:182794/149‑1 (MQ=255) tGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAggt > 3:268656/1‑121 (MQ=255) tGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAggt < 4:268656/121‑1 (MQ=255) gATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCACGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGa > 5:332903/1‑149 (MQ=255) tCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACg > 4:353693/1‑149 (MQ=255) tCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACg > 8:69845/1‑149 (MQ=255) cGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGc > 8:222955/1‑149 (MQ=255) gCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACGTGTTCTACCCCGAGGCGACGCt > 7:384392/1‑149 (MQ=255) aGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTg > 3:224383/1‑148 (MQ=255) gggCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCa < 8:269409/149‑1 (MQ=255) gggAAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGGGAGGAGAACTGTGCCGAAGCCTATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCa < 5:281126/149‑1 (MQ=255) ggCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCAGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCAg > 2:91610/1‑149 (MQ=255) gCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCAgc > 2:348754/1‑149 (MQ=255) cAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGAc > 6:158910/1‑120 (MQ=255) cAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGAc < 5:158910/120‑1 (MQ=255) tcctGACCCAGGGTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCAGCGCa > 4:5271/4‑149 (MQ=255) tcctGACCCAGGGTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCAGCGCa > 5:152361/4‑149 (MQ=255) tcctGACCCAGGGTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCAGCGCa > 8:231329/4‑149 (MQ=255) tcctGACCCAGGGTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCAGCGCa > 8:225217/4‑149 (MQ=255) ctGACCCAGGGTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGtt > 1:98862/2‑121 (MQ=255) ctGACCCAGGGTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCAGCGCagg > 6:2548/2‑147 (MQ=255) gACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCAGCGCATgg > 2:433737/1‑148 (MQ=255) ccacccTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCAGCGCATGGCAGc > 4:286584/1‑149 (MQ=255) ccacccTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCAGCGCATGGCAGc > 3:294867/1‑149 (MQ=255) cacccTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGTTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCAGCGCATGGCAGCg > 1:113078/1‑149 (MQ=255) acccTGGGTCAGGACGCTGGCGAGTGTGGAGAACTGTGGCGAAGCCTATGTGCGCCTGTTACTGCGCATGTTGGCCGGGGTGGGGCTGGTGGATCAGGTTGTGCGCGACTTGtt < 7:78473/114‑1 (MQ=255) | TGCTGGATGAACTGCTCGACGACAGCGCCATCGCCAGCAGCGGGCCATGGCTGCATCTGCCCGATCACAAGGTACAGTTGAGCGAAGCCGACAGTGCATTGTGGGCACGTCTGCAGCCGAAGTTGATCGCAGGGCAATTTGACCCACCCTGGGTCAGGACGCTGGCGAGTGAGGAGAACTGTGCCGAAGCCGATGTGCGCCTGTTACTGCGCAAGTTGGCCCGGCTGGGCCTGGTGCATCAGGTGGTGCGCGACTTGTTCTACCCCGAGGCGACGCTGCAGCGCATGGCAGCG > NC_002947/583573‑583865 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |