BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	632,040	C→T	6.0%	A467A (GCG→GCA)	aldB‑I ←	aldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	632,040	0	C	T	6.0%	305.9 / 5.2	100	A467A (GCG→GCA)	aldB‑I	aldehyde dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (46/48); new base T (3/3); total (49/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01

Only 100 of 104 total aligned reads displayed.

GTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTCGTTGGCAATTGCC  >  NC_002947/631893‑632181

gTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAAcg                                                                                                                                              >  1:185280/1‑149 (MQ=255)

 tttACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAAcgc                                                                                                                                             <  8:376141/149‑1 (MQ=255)

   tACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAAcgcg                                                                                                                                            <  8:142179/148‑1 (MQ=255)

          gaagaaGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATgcgc                                                                                                                                    >  1:191838/1‑149 (MQ=255)

             gaagGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCgg                                                                                                                                 <  8:192729/149‑1 (MQ=255)

             gaagGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCgg                                                                                                                                 <  7:248307/149‑1 (MQ=255)

                ggCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGt                                                                                                                               >  8:147483/1‑148 (MQ=255)

                ggCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTa                                                                                                                              >  5:258278/1‑149 (MQ=255)

                 gCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTa                                                                                                                              >  5:43074/1‑148 (MQ=255)

                  cGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACa                                                                                                                            <  6:342635/149‑1 (MQ=255)

                   gACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGGCGCATGCCGCGTTCGGCGGGTACaa                                                                                                                           <  4:405379/149‑2 (MQ=17)

                   gACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGGCGCATGCCGCGTTCGGCGGGTACaa                                                                                                                           <  1:188621/149‑2 (MQ=17)

                   gACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAg                                                                                                                           >  7:45689/1‑149 (MQ=255)

                      gcgGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAggtg                                                                                                                        <  5:97680/149‑1 (MQ=255)

                       cgGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAggtgg                                                                                                                       <  3:246269/149‑1 (MQ=255)

                         gTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCggg                                                                                                                                >  4:286256/1‑138 (MQ=255)

                         gTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCggg                                                                                                                                <  3:286256/138‑1 (MQ=255)

                              gaagaaGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGt                                                                                                                <  8:377776/149‑1 (MQ=255)

                              gaagaaGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGt                                                                                                                <  7:97686/149‑1 (MQ=255)

                               aagaagCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGtt                                                                                                               <  5:229346/149‑1 (MQ=255)

                               aagaagCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGtt                                                                                                               <  6:43074/149‑1 (MQ=255)

                               aagaagCCCAGCGGATTGATGTCGTAGCTCACAAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGAACAGCTGGTAGAAg                                                                                                                 >  8:107338/1‑147 (MQ=255)

                                agaagCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTg                                                                                                              <  7:111078/149‑1 (MQ=255)

                                 gaagCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTgg                                                                                                             >  3:407860/1‑149 (MQ=255)

                                 gaagCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTgg                                                                                                             <  8:250220/149‑1 (MQ=255)

                                 gaagCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTCATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTgg                                                                                                             <  5:53404/149‑1 (MQ=255)

                                   agCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGt                                                                                                            <  2:191838/148‑1 (MQ=255)

                                        aGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCcacac                                                                                                      <  7:147483/149‑1 (MQ=255)

                                                   gTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCt                                                                                           >  6:93511/1‑149 (MQ=255)

                                                    tCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTAc                                                                                                                             >  3:198917/1‑114 (MQ=255)

                                                    tCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTAc                                                                                                                             <  4:198917/114‑1 (MQ=255)

                                                     cGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCtt                                                                                          >  5:302962/1‑148 (MQ=255)

                                                           tCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCCCAGAACCCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCAAGCCTTGATGccc                                                                                   >  3:290773/1‑149 (MQ=255)

                                                               cagcagGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCgcc                                                                               >  6:56382/1‑147 (MQ=255)

                                                                agcagGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGc                                                                               >  2:377452/1‑148 (MQ=255)

                                                                agcagGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGc                                                                               <  1:377452/148‑1 (MQ=255)

                                                                 gcagGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGCCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGcc                                                                              >  8:277892/1‑148 (MQ=255)

                                                                  cagGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCa                                                                            >  1:117088/1‑149 (MQ=255)

                                                                   agGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCAt                                                                           <  6:203028/149‑1 (MQ=255)

                                                                      ttCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCg                                                                        >  7:163641/1‑149 (MQ=255)

                                                                      ttCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCg                                                                        >  3:162737/1‑149 (MQ=255)

                                                                       tCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCgg                                                                       >  8:122650/1‑149 (MQ=255)

                                                                           ggTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGt                                                                      <  6:402963/146‑1 (MQ=255)

                                                                           ggTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTa                                                                     <  3:94809/147‑1 (MQ=255)

                                                                             tCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCa                                                                 >  1:436585/1‑149 (MQ=255)

                                                                                  tgATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGtt                                                            >  5:395293/1‑149 (MQ=255)

                                                                                   gATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGtt                                                            >  4:285556/1‑148 (MQ=255)

                                                                                    aTAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGtt                                                            <  6:302962/147‑1 (MQ=255)

                                                                                     tAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGa                                                          <  2:117088/148‑1 (MQ=255)

                                                                                     tAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGAt                                                         <  2:185280/149‑1 (MQ=255)

                                                                                     tAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGAt                                                         <  6:24255/149‑1 (MQ=255)

                                                                                      aGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATg                                                        >  2:434058/1‑149 (MQ=255)

                                                                                          aTCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTcgc                                                    <  3:93909/149‑1 (MQ=255)

                                                                                          aTCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTcgc                                                    <  7:122650/149‑1 (MQ=255)

                                                                                          aTCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTcgc                                                    <  4:407860/149‑1 (MQ=255)

                                                                                          aTCCAGCATCATCTTGTGCGCCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTcgc                                                    <  7:277892/149‑1 (MQ=255)

                                                                                             cAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACATGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTcgc                                                    <  8:30762/146‑1 (MQ=255)

                                                                                             cAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGACGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTcgcgcg                                                 >  7:30762/1‑147 (MQ=255)

                                                                                               gCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTc                                               <  8:32345/149‑1 (MQ=255)

                                                                                                catcatCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCGGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTcc                                              <  6:257380/149‑1 (MQ=255)

                                                                                                catcatCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTc                                               >  8:22906/1‑148 (MQ=255)

                                                                                                catcatCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCAGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTcc                                              <  8:163641/149‑1 (MQ=255)

                                                                                                 atcatcTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCca                                             >  3:375952/1‑149 (MQ=255)

                                                                                                   catcTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGcc                                                                                            >  2:391658/1‑100 (MQ=255)

                                                                                                   catcTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGcc                                                                                            <  1:391658/100‑1 (MQ=255)

                                                                                                   catcTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCcaca                                           >  3:133676/1‑149 (MQ=255)

                                                                                                     tcTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACg                                         >  3:385838/1‑149 (MQ=255)

                                                                                                      cTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACATACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGc                                        >  8:311722/1‑149 (MQ=255)

                                                                                                      cTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGAGGGTCCACACGc                                        >  8:152263/1‑149 (MQ=255)

                                                                                                       ttGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGcc                                       >  3:122498/1‑149 (MQ=255)

                                                                                                       ttGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGcc                                       >  4:92265/1‑149 (MQ=255)

                                                                                                        tgtgCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCg                                      >  6:277524/1‑149 (MQ=255)

                                                                                                         gtgCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGATGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGcc                                       <  4:226046/147‑1 (MQ=255)

                                                                                                            cGTCTCACGCCCATCCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTTGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGgcg                                   <  7:107338/148‑1 (MQ=255)

                                                                                                               ctcACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCa                               <  5:74994/149‑1 (MQ=255)

                                                                                                                  aCGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGc                            >  6:383062/1‑149 (MQ=255)

                                                                                                                      ccAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGaa                        >  2:401067/1‑149 (MQ=255)

                                                                                                                          cccccGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCg                    <  1:401067/149‑1 (MQ=255)

                                                                                                                            cccGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGgt                  >  8:409884/1‑149 (MQ=255)

                                                                                                                            cccGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGgt                  <  4:122498/149‑1 (MQ=255)

                                                                                                                             ccGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGgtg                 <  7:22906/149‑1 (MQ=255)

                                                                                                                              cGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGgt                  >  4:243611/1‑147 (MQ=255)

                                                                                                                                   tcttGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTCGTTg           >  2:36454/1‑149 (MQ=255)

                                                                                                                                    cttGTACCCTCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTCGTTg           <  6:258278/148‑1 (MQ=255)

                                                                                                                                     ttGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAgg                             <  3:32243/129‑1 (MQ=255)

                                                                                                                                     ttGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAgg                             >  4:32243/1‑129 (MQ=255)

                                                                                                                                     ttGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTCGTTgg          >  2:281886/1‑148 (MQ=255)

                                                                                                                                     ctGTACCCGGCGCATGCCGCGTTCGGCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGGCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTc               <  4:43068/142‑1 (MQ=18)

                                                                                                                                     ctGTACCCGGCGCATGCCGCGTTCGGCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTc               >  3:43068/2‑143 (MQ=18)

                                                                                                                                      tGTACCCGGCGCATGCCGCGTTCGGCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTCGTTGGc         >  1:12704/1‑148 (MQ=17)

                                                                                                                                       gTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCgg                                                                       <  2:10416/85‑1 (MQ=255)

                                                                                                                                       gTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCgg                                                                       >  1:10416/1‑85 (MQ=255)

                                                                                                                                       gTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCACGCCCAACTCGGTGTCGTTGGCaa       >  4:46438/1‑149 (MQ=255)

                                                                                                                                        tACCCGGCGCATGCCGCGTTCGGCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTCGTTGGCAAt      >  6:156487/1‑149 (MQ=18)

                                                                                                                                          cccGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTCGTTGGCAAtt     <  2:436585/148‑1 (MQ=255)

                                                                                                                                          cccGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTCGTTGGCAATTg    <  4:385838/149‑1 (MQ=255)

                                                                                                                                           ccgccgAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTCGTTGGCAATTGc   >  3:88392/1‑149 (MQ=255)

                                                                                                                                           ccgccgAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTCGTTGGCAATTGc   <  5:93511/149‑1 (MQ=255)

                                                                                                                                            cgccgAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTCGTTGGCAATTGcc  <  5:277524/149‑1 (MQ=255)

                                                                                                                                             gccgAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTCGTTGGCAATTGc   >  7:267784/1‑147 (MQ=255)

GTTTACCCGCGAAGAAGGCGACGCGGTCTAGAAGAAGCCCAGCGGATTGATGTCGTAGCTCACCAGCAGGTTCTTGGTCTGCTGATAGTGATCCAGCATCATCTTGTGCGTCTCACGCCCAACCCCCGACTTCTTGTACCCGCCGAACGCGGCATGCGCCGGGTACAGGTGGTAGCAGTTGGTCCACACACGGCCAGCCTTGATGCCCCGGCCCATGCGGTAGGCACGGTTGATGTCGCGGGTCCACACGCCGGCGCCCAGGCCGAACTCGGTGTCGTTGGCAATTGCC  >  NC_002947/631893‑632181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`