Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 708,759 A→G 5.2% A387A (GCA→GCG)  murJ → lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947708,7590AG 5.2% 290.1 / 3.6 96A387A (GCA→GCG) murJlipid II flippase
Reads supporting (aligned to +/- strand):  ref base A (42/49);  new base G (2/3);  total (44/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

AATATGGCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGC  >  NC_002947/708611‑708907
                                                                                                                                                    |                                                                                                                                                    
aaTATGGCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGGTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCa                                                                                                                                                      <  8:133544/149‑1 (MQ=255)
aaTATGGCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCa                                                                                                                                                      <  3:83091/149‑1 (MQ=255)
aaTATGGCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCa                                                                                                                                                      <  6:279146/149‑1 (MQ=255)
    tGGCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTc                                                                                                                                                   <  2:227346/148‑1 (MQ=255)
      gCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGATTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCtt                                                                                                                                                 >  5:250565/1‑148 (MQ=255)
        aaGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCAc                                                                                                                                              >  4:311016/1‑149 (MQ=255)
         aGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCAcc                                                                                                                                             <  1:162570/149‑1 (MQ=255)
          gTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGACTGCGATCTTCAccg                                                                                                                                            <  4:387163/149‑2 (MQ=255)
             cAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTGTTCACCCTg                                                                                                                                          <  4:261503/148‑1 (MQ=255)
              aGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGt                                                                                                                                        <  4:86789/149‑1 (MQ=255)
               gcgcGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGCCACCTGGCTTCTATCCGCAGCAGAATATTCCCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTc                                                                                                                                       <  6:110072/149‑1 (MQ=255)
                cgcgTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTc                                                                                                                                       <  4:111092/148‑1 (MQ=255)
                cgcgTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCt                                                                                                                                      >  6:146719/1‑149 (MQ=255)
                cgcgTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCt                                                                                                                                      >  2:351424/1‑149 (MQ=255)
                  cgTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTg                                                                                                                                     >  5:68998/1‑148 (MQ=255)
                   gTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCATGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCa                                                                                                                                   <  2:149856/149‑1 (MQ=255)
                    tttGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCAc                                                                                                                                  >  8:258796/1‑149 (MQ=255)
                       gATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCa                                                                                                                               >  8:119430/1‑149 (MQ=255)
                        aTGCGGCGATGACCCAGCGGGCGTTGAGCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAg                                                                                                                              <  2:141252/149‑1 (MQ=255)
                         tGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAg                                                                                                                              <  6:250565/148‑1 (MQ=255)
                             gcgATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGtt                                                                                                                         <  8:324357/149‑1 (MQ=255)
                             gcgATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGtt                                                                                                                         <  5:146719/149‑1 (MQ=255)
                              cgATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTc                                                                                                                        <  5:95426/149‑1 (MQ=255)
                              cgATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTc                                                                                                                        <  6:136490/149‑1 (MQ=255)
                                 tGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAAc                                                                                                                     <  5:279000/149‑1 (MQ=255)
                                  gACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAAcc                                                                                                                    >  5:394840/1‑149 (MQ=255)
                                   aCCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAAcc                                                                                                                    <  6:394840/148‑1 (MQ=255)
                                    cccAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCtt                                                                                                                  >  2:97848/1‑149 (MQ=255)
                                    cccAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCtt                                                                                                                  >  7:162306/1‑149 (MQ=255)
                                     ccAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTg                                                                                                                 >  3:323062/1‑149 (MQ=255)
                                     ccAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTg                                                                                                                 <  1:111901/149‑1 (MQ=255)
                                       aGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGcc                                                                                                               <  2:413918/149‑1 (MQ=255)
                                       aGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGcc                                                                                                               <  7:119430/149‑1 (MQ=255)
                                           ggCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTgg                                                                                                           <  1:351424/149‑1 (MQ=255)
                                            gCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGt                                                                                                          <  1:170580/149‑1 (MQ=255)
                                            gCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGt                                                                                                          <  4:199250/149‑1 (MQ=255)
                                             cGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTc                                                                                                         >  1:169577/1‑149 (MQ=255)
                                                   tCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGAACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCg                                                                                                   >  4:121661/1‑149 (MQ=255)
                                                     gCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGc                                                                                                  <  3:71994/148‑1 (MQ=255)
                                                       cTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTg                                                                                               <  7:2373/149‑1 (MQ=255)
                                                          ttCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGca                                                                                             >  2:153338/1‑148 (MQ=255)
                                                             cGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACAt                                                                                          <  7:258796/148‑1 (MQ=255)
                                                              gTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGc                                                                                        >  5:118269/1‑149 (MQ=255)
                                                                 ggCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCgg                                                                                     >  3:210441/1‑149 (MQ=255)
                                                                  gCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGc                                                                                    <  8:162306/149‑1 (MQ=255)
                                                                  gCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGACTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTCCACCTCCCGGc                                                                                    >  8:402683/1‑149 (MQ=255)
                                                                        tGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCa                                                                              >  2:379902/1‑149 (MQ=255)
                                                                          gCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCAtt                                                                            <  6:77176/149‑1 (MQ=255)
                                                                            gATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTgg                                                                          >  5:343382/1‑149 (MQ=255)
                                                                            gATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTgg                                                                          >  1:55595/1‑149 (MQ=255)
                                                                            gATCATTCTGATCAAGGTACTGGCACCTGGCTTCTAAGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTgg                                                                          >  1:70987/1‑149 (MQ=255)
                                                                            gATCATTCTGATCAAGGTACTGGCACCTGCCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTgg                                                                          <  4:376141/149‑1 (MQ=255)
                                                                             aTCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGc                                                                         <  1:153338/149‑1 (MQ=255)
                                                                                 ttCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCAGGCCGGCAtgtgc                                                                         <  8:297269/145‑5 (MQ=255)
                                                                                 ttCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCAGGCCGGCAtgtgc                                                                         >  7:297269/1‑141 (MQ=255)
                                                                                  tCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGAt                                                                      >  4:62895/1‑147 (MQ=255)
                                                                                      aTCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCt                                                                >  7:12928/1‑149 (MQ=255)
                                                                                          aGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGGAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGt                                                            >  6:100305/1‑149 (MQ=255)
                                                                                          aGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGt                                                            >  1:310951/1‑149 (MQ=255)
                                                                                            gTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGtgc                                                          <  3:62895/149‑1 (MQ=255)
                                                                                            gTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGtgc                                                          <  2:70987/149‑1 (MQ=255)
                                                                                               cTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGtgcctg                                                       <  8:12928/149‑1 (MQ=255)
                                                                                                  gCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGtgcctgcc                                                     <  4:323062/148‑1 (MQ=255)
                                                                                                  gCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTgcctgcct                                                    >  1:433266/1‑149 (MQ=255)
                                                                                                  gCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTgcctgcct                                                    >  4:273176/1‑149 (MQ=255)
                                                                                                   cACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTgcctgcct                                                    >  2:292928/1‑148 (MQ=255)
                                                                                                   cACCTGGCTTCTATGCGCACCAGAATATTAGCACGCCGTTGAAGATCGCAGTCTTCACCATGGTCTGAACCCAGCTGTTCAACCTTCCCCTGGTCGGCCCGCTTGCACATCACGCCCTGGCATTGGCGATCAGACTCGGaccctgc                                                      >  2:379863/1‑146 (MQ=255)
                                                                                                    aCCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGcctgcctg                                                   >  4:224609/1‑148 (MQ=255)
                                                                                                     ccTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGaa                                                 >  3:257159/1‑149 (MQ=255)
                                                                                                      cTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAAt                                                >  1:255162/1‑149 (MQ=255)
                                                                                                      cTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAAt                                                >  8:24285/1‑149 (MQ=255)
                                                                                                           ttCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCgg                                           >  5:184972/1‑149 (MQ=255)
                                                                                                           ttCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCgg                                           >  2:426844/1‑149 (MQ=255)
                                                                                                             cTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGtt                                         >  8:28790/1‑149 (MQ=255)
                                                                                                             cTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGt                                          <  1:379902/148‑1 (MQ=255)
                                                                                                             cTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGt                                          <  3:332614/148‑1 (MQ=255)
                                                                                                               aTGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTg                                       >  4:267583/1‑149 (MQ=255)
                                                                                                                tgggcaccaGAATATTCGCACTCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCAGCCAGCTGTTCAACCTTGCCCTGGGCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGtt                                         <  7:402683/143‑1 (MQ=255)
                                                                                                                tGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGttt                                        <  7:353987/147‑1 (MQ=255)
                                                                                                                tGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTg                                       <  6:343382/148‑1 (MQ=255)
                                                                                                                     agcagAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTc                                 <  2:45459/149‑1 (MQ=255)
                                                                                                                      gcagAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCt                                <  2:169577/149‑1 (MQ=255)
                                                                                                                      gcagAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCt                                <  3:121661/149‑1 (MQ=255)
                                                                                                                        agAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTg                               >  7:141472/1‑148 (MQ=255)
                                                                                                                           atatTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGaa                            <  6:68998/148‑1 (MQ=255)
                                                                                                                               tCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTgc                       >  5:365947/1‑149 (MQ=255)
                                                                                                                                 gCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTgcg                      <  7:24285/148‑1 (MQ=255)
                                                                                                                                  cACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCa                    <  6:118269/149‑1 (MQ=255)
                                                                                                                                  cACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCa                    <  3:311016/149‑1 (MQ=255)
                                                                                                                                   accagggTGAAGACTGCGATCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGttt                                        >  7:225904/6‑128 (MQ=255)
                                                                                                                                   accagggTGAAGACTGCGATCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGttt                                        <  8:225904/123‑1 (MQ=255)
                                                                                                                                    cGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGt                  <  4:92631/149‑1 (MQ=255)
                                                                                                                                    cGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGt                  <  3:273176/149‑1 (MQ=255)
                                                                                                                                      agggTGAAGACTGCGATCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTgcctgcct                                                    <  7:361861/111‑1 (MQ=255)
                                                                                                                                      agggTGAAGACTGCGATCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTgcctgcct                                                    >  8:361861/3‑113 (MQ=255)
                                                                                                                                       cGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTcag               >  3:348819/1‑149 (MQ=255)
                                                                                                                                         gTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTcag               >  3:64412/1‑147 (MQ=255)
                                                                                                                                          tGAAGATCGCATTCTTCAGCCTGGTCTGCACCGAGCTGTTCAAACTTGCCCTGGTCGGCCCTCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTcagc              <  1:411740/147‑1 (MQ=255)
                                                                                                                                            aaGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCt          <  2:55595/149‑1 (MQ=255)
                                                                                                                                                    aGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGc  <  2:255162/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AATATGGCAAGTTCAGCGCGTTTGATGCGGCGATGACCCAGCGGGCGTTGATCGCCTATTCCGTGGGCTTGCTGGCGATCATTCTGATCAAGGTACTGGCACCTGGCTTCTATGCGCAGCAGAATATTCGCACGCCGGTGAAGATCGCAGTCTTCACCCTGGTCTGCACCCAGCTGTTCAACCTTGCCCTGGTCGGCCCGCTTGCACATGCCGGCCTGGCATTGGCGATCAGCCTCGGTGCCTGCCTGAATGCCGGTTTGCTGTTCTGGAAGCTGCGCAGTCAGCAGCTGTTCGAGC  >  NC_002947/708611‑708907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑