Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 711,079 C→G 5.9% Q293E (CAA→GAA)  ileS → isoleucine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947711,0790CG 5.9% 332.4 / 8.9 102Q293E (CAA→GAA) ileSisoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (43/53);  new base G (3/3);  total (46/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.04e-01

Only 100 of 103 total aligned reads displayed.

CCGGCCAACCAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACC  >  NC_002947/710935‑711225
                                                                                                                                                |                                                                                                                                                   
ccGGCCAACCCTGCTCTGAACATCCACCCGGAAGTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAg                                                                                                                                                  <  8:367329/148‑1 (MQ=255)
ccGGCCAACCAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAgg                                                                                                                                                 >  5:233611/1‑149 (MQ=255)
 cGGCCAACCAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGt                                                                                                                                                >  3:175794/1‑149 (MQ=255)
     cAACCAGGCTCTGAACATCAACCCGGAATTCAAGTACGCCCTGGTCTACACCGGCGAGCGCCTGCTGGTGCTGGCTTAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCgg                                                                                                                                            <  1:131894/149‑1 (MQ=255)
        ccAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCGAACCTTGGgcgc                                                                                                                                         >  6:16292/1‑149 (MQ=255)
         cAGGCTCTGAACATCCACCCGGAATTCATGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCt                                                                                                                                        >  2:129122/1‑149 (MQ=255)
           ggCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCGAACCTTGGGCGCTgg                                                                                                                                      <  4:186552/149‑1 (MQ=255)
           ggCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCGAACCTTGGGCGCTgg                                                                                                                                      <  5:16292/149‑1 (MQ=255)
           ggCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTAGAAGGTTCGGTTGTTGCCAGCGCCGAACCTTGGGCGCTgg                                                                                                                                      <  2:406414/149‑1 (MQ=255)
            gCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCaa                                                                                                                                                   <  4:202575/135‑1 (MQ=255)
            gCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCaa                                                                                                                                                   >  3:202575/1‑135 (MQ=255)
              tctGAACATCCACACGGAATTCAAGTACGCCCTGGTAGACACCGGCGAGCGCCTGCTGGTGCTGGCAGAAGACCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGTTTCGGTTGTTGCAAGAGCCCAAGGTTCGGCGCTCGAGc                                                                                                                                   >  1:395094/1‑149 (MQ=255)
                tGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTg                                                                                                                                       <  5:162077/143‑1 (MQ=255)
                tGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTg                                                                                                                                       >  6:162077/1‑143 (MQ=255)
                  aaCATCCCCCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGAt                                                                                                                               <  3:377773/149‑1 (MQ=255)
                  aaCATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCt                                                                                                                                  <  4:370850/146‑1 (MQ=255)
                       ccACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTg                                                                                                                                       <  5:37764/136‑1 (MQ=255)
                       ccACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTg                                                                                                                                       >  6:37764/1‑136 (MQ=255)
                        cACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGTCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACtt                                                                                                                        <  4:208621/149‑1 (MQ=255)
                        cACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACtt                                                                                                                        <  2:374231/149‑1 (MQ=255)
                         aCCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTc                                                                                                                       >  7:81848/1‑149 (MQ=255)
                          cccGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTCGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTcc                                                                                                                      >  7:281925/1‑149 (MQ=255)
                           ccGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCg                                                                                                                     >  1:179403/1‑149 (MQ=255)
                            cGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCg                                                                                                                     >  8:231914/1‑148 (MQ=255)
                            cGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCg                                                                                                                     <  7:231914/148‑1 (MQ=255)
                            cGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGt                                                                                                                    >  4:224627/1‑149 (MQ=255)
                             ggAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTc                                                                                                                   <  2:232628/149‑1 (MQ=255)
                               aaTTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCAt                                                                                                                 <  7:155199/149‑1 (MQ=255)
                               aaTTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCAt                                                                                                                 <  7:161111/149‑1 (MQ=255)
                                 ttCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATcc                                                                                                               >  3:203168/1‑149 (MQ=255)
                                   cAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGt                                                                                                             >  7:21451/1‑149 (MQ=255)
                                    aaGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGtt                                                                                                            <  6:430071/149‑1 (MQ=255)
                                    aaGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGGTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGtt                                                                                                            <  5:337036/149‑1 (MQ=255)
                                      gTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTc                                                                                                           >  5:953/1‑148 (MQ=255)
                                       tACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTa                                                                                                         <  6:233611/149‑1 (MQ=255)
                                            ccTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGgc                                                                                                                                           <  1:386675/111‑1 (MQ=255)
                                            ccTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGgc                                                                                                                                           >  2:386675/1‑111 (MQ=255)
                                                gTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGc                                                                                                  <  1:257483/147‑1 (MQ=255)
                                                  cGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGt                                                                                              <  3:224627/149‑1 (MQ=255)
                                                   gACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGt                                                                                              <  3:216206/148‑1 (MQ=255)
                                                   gACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGt                                                                                              <  3:209239/148‑1 (MQ=255)
                                                   gACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTc                                                                                             >  5:177334/1‑149 (MQ=255)
                                                    acacCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCg                                                                                            <  4:371733/149‑1 (MQ=255)
                                                     cacCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGc                                                                                           >  4:161778/1‑149 (MQ=255)
                                                      acCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGc                                                                                                  >  6:178413/1‑141 (MQ=255)
                                                      acCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGc                                                                                                  <  5:178413/141‑1 (MQ=255)
                                                          gCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATc                                                                                      >  3:100833/1‑149 (MQ=255)
                                                              gcgcCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTAcc                                                                                  <  4:175794/149‑1 (MQ=255)
                                                              gcgcCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTAcc                                                                                  >  8:170147/1‑149 (MQ=255)
                                                              gcgcCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTAcc                                                                                  >  7:256164/1‑149 (MQ=255)
                                                                ggctggtgGTGCTGGCTGTATAGCTTGTCGCTTCGTTGCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTg                                                                                <  3:212843/147‑1 (MQ=255)
                                                                  ctgctgGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGc                                                                              >  5:247414/1‑149 (MQ=255)
                                                                      tggtgctggCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGa                                                                                                                                <  5:248149/96‑1 (MQ=255)
                                                                      tggtgctggCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGa                                                                                                                                >  6:248149/1‑96 (MQ=255)
                                                                       ggtgctggCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACt                                                                         <  6:151461/149‑1 (MQ=255)
                                                                          gctggctgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACg                                                                      >  1:302829/1‑149 (MQ=255)
                                                                              gctgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGATTACGTc                                                                    <  2:364999/147‑1 (MQ=255)
                                                                              gctgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTc                                                                    >  7:326748/1‑147 (MQ=255)
                                                                               ctgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAg                                                                 <  8:281925/149‑1 (MQ=255)
                                                                               ctgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAg                                                                 <  4:203168/149‑1 (MQ=255)
                                                                                tgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGc                                                                <  5:175261/149‑1 (MQ=255)
                                                                                  aaGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTg                                                              <  1:129122/149‑1 (MQ=255)
                                                                                   agagCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTgg                                                             <  6:247414/149‑1 (MQ=255)
                                                                                   agagCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTgg                                                             <  6:177334/149‑1 (MQ=255)
                                                                                   agagCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTg                                                              >  8:4137/1‑148 (MQ=255)
                                                                                   agagCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTg                                                              <  7:4137/148‑1 (MQ=255)
                                                                                    gagCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTAGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTggg                                                            >  3:218085/1‑149 (MQ=255)
                                                                                     agCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGa                                                                           >  3:120855/1‑133 (MQ=255)
                                                                                     agCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGa                                                                           <  4:120855/133‑1 (MQ=255)
                                                                                       cTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGgcgc                                                         <  6:371515/149‑1 (MQ=255)
                                                                                         ggTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCg                                                       >  8:320999/1‑149 (MQ=255)
                                                                                          gtcgagtcgaGCCTGAAGCGCTACAACCTTGAACGTACGGTTCTTGCCAGAGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCCTTCTACGATCGACTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCgg                                                      >  2:340691/1‑149 (MQ=255)
                                                                                              aGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCAACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTAcc                                                  <  2:61179/149‑1 (MQ=255)
                                                                                                   tGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCAAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgt                                             <  6:953/149‑1 (MQ=255)
                                                                                                     ccTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgtg                                            <  5:52693/148‑1 (MQ=255)
                                                                                                     ccTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgtg                                            <  7:170147/148‑1 (MQ=255)
                                                                                                     ccTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgccc                                           <  7:330072/149‑4 (MQ=255)
                                                                                                      cTGAAGCGCTACAACCTGGAAGGTTCGGGTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGGCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgtggt                                          <  6:221880/149‑1 (MQ=255)
                                                                                                        gAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgtggtg                                         >  2:404262/1‑148 (MQ=255)
                                                                                                            cgcTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTc                                    >  2:353480/1‑149 (MQ=255)
                                                                                                             gcTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTcc                                   <  5:92304/149‑1 (MQ=255)
                                                                                                                   aCCTGGAAGGTTCGGTTGTTGCAAGCGCCCAAGGTTAGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCAGGTACCGGCGAGGTGCATTCCTCga                               >  6:452/1‑146 (MQ=255)
                                                                                                                    ccTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGATCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTAAGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCAGGTAACGGCCTGCTGCATTCCTCGCAAg                            >  7:184602/1‑149 (MQ=255)
                                                                                                                     cTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCg                                                       >  7:224759/1‑121 (MQ=255)
                                                                                                                     cTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCg                                                       <  8:224759/121‑1 (MQ=255)
                                                                                                                            gTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGt                    <  2:302829/149‑1 (MQ=255)
                                                                                                                             ttCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGt                    >  3:197863/1‑148 (MQ=255)
                                                                                                                                gggtgttgCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGGGGTGCATTCCTCGCCAGCCTACGGTGaa                 <  4:391736/145‑1 (MQ=255)
                                                                                                                                 gttgttGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAga               >  8:1085/1‑149 (MQ=255)
                                                                                                                                 gttgttGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAga               >  7:21432/1‑149 (MQ=255)
                                                                                                                                  ttgttgCCAGCTCCCAAGGTTCGGCGCTGTAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGATCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACTGTGAAgac              <  5:303882/149‑1 (MQ=255)
                                                                                                                                     ttgCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAgccga            >  8:174928/1‑148 (MQ=255)
                                                                                                                                     ttgCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAgacga            <  7:320999/148‑1 (MQ=255)
                                                                                                                                       tccAGCGCCGAACCTTGGGCGCTGGAGCTGACCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACtt         >  3:60059/2‑149 (MQ=255)
                                                                                                                                        ccAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTc        >  6:12087/1‑149 (MQ=255)
                                                                                                                                          aGCGCCCAAGGTTCGGCGCTGGAGCTTATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACt          <  2:166077/145‑1 (MQ=255)
                                                                                                                                          aGCGCCCAAGGTTCGGCGCTGGAGCTTATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACt          >  1:166077/1‑145 (MQ=255)
                                                                                                                                          aGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGt      <  1:404262/149‑1 (MQ=255)
                                                                                                                                           gcgcCGAACCTTGGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGt      >  2:704/1‑148 (MQ=255)
                                                                                                                                               ccAAGGTTCGGCGCTGGAGCTGATCAACTTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCAcc  >  1:259507/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CCGGCCAACCAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAACTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACC  >  NC_002947/710935‑711225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: