BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	711,086	C→G	5.9%	S295W (TCG→TGG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	711,086	0	C	G	5.9%	328.3 / 9.1	101	S295W (TCG→TGG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base C (41/53); new base G (3/3); total (45/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.41e-01

Only 100 of 101 total aligned reads displayed.

CAACCAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACCTGCAA  >  NC_002947/710940‑711230

cAACCAGGCTCTGAACATCAACCCGGAATTCAAGTACGCCCTGGTCTACACCGGCGAGCGCCTGCTGGTGCTGGCTTAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCgg                                                                                                                                                 <  1:131894/149‑1 (MQ=255)

   ccAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCGAACCTTGGgcgc                                                                                                                                              >  6:16292/1‑149 (MQ=255)

    cAGGCTCTGAACATCCACCCGGAATTCATGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCt                                                                                                                                             >  2:129122/1‑149 (MQ=255)

      ggCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCGAACCTTGGGCGCTgg                                                                                                                                           <  4:186552/149‑1 (MQ=255)

      ggCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCGAACCTTGGGCGCTgg                                                                                                                                           <  5:16292/149‑1 (MQ=255)

      ggCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTAGAAGGTTCGGTTGTTGCCAGCGCCGAACCTTGGGCGCTgg                                                                                                                                           <  2:406414/149‑1 (MQ=255)

         tctGAACATCCACACGGAATTCAAGTACGCCCTGGTAGACACCGGCGAGCGCCTGCTGGTGCTGGCAGAAGACCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGTTTCGGTTGTTGCAAGAGCCCAAGGTTCGGCGCTCGAGc                                                                                                                                        >  1:395094/1‑149 (MQ=255)

           tGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTg                                                                                                                                            <  5:162077/143‑1 (MQ=255)

           tGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTg                                                                                                                                            >  6:162077/1‑143 (MQ=255)

             aaCATCCCCCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGAt                                                                                                                                    <  3:377773/149‑1 (MQ=255)

             aaCATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCt                                                                                                                                       <  4:370850/146‑1 (MQ=255)

                  ccACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTg                                                                                                                                            <  5:37764/136‑1 (MQ=255)

                  ccACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTg                                                                                                                                            >  6:37764/1‑136 (MQ=255)

                   cACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGTCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑tt                                                                                                                             <  4:208621/149‑1 (MQ=255)

                   cACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑tt                                                                                                                             <  2:374231/149‑1 (MQ=255)

                    aCCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTc                                                                                                                            >  7:81848/1‑149 (MQ=255)

                     cccGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTCGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTcc                                                                                                                           >  7:281925/1‑149 (MQ=255)

                      ccGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCg                                                                                                                          >  1:179403/1‑149 (MQ=255)

                       cGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCg                                                                                                                          >  8:231914/1‑148 (MQ=255)

                       cGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCg                                                                                                                          <  7:231914/148‑1 (MQ=255)

                       cGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGt                                                                                                                         >  4:224627/1‑149 (MQ=255)

                       cGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGt                                                                                                                         <  3:280704/149‑1 (MQ=255)

                        ggAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTc                                                                                                                        <  2:232628/149‑1 (MQ=255)

                          aaTTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCAt                                                                                                                      <  7:161111/149‑1 (MQ=255)

                          aaTTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCAt                                                                                                                      <  7:155199/149‑1 (MQ=255)

                            ttCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATcc                                                                                                                    >  3:203168/1‑149 (MQ=255)

                              cAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGt                                                                                                                  >  7:21451/1‑149 (MQ=255)

                               aaGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGtt                                                                                                                 <  6:430071/149‑1 (MQ=255)

                               aaGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGGTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGtt                                                                                                                 <  5:337036/149‑1 (MQ=255)

                                 gTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTc                                                                                                                >  5:953/1‑148 (MQ=255)

                                  tACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTa                                                                                                              <  6:233611/149‑1 (MQ=255)

                                       ccTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGgc                                                                                                                                                <  1:386675/111‑1 (MQ=255)

                                       ccTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGgc                                                                                                                                                >  2:386675/1‑111 (MQ=255)

                                           gTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGc                                                                                                       <  1:257483/147‑1 (MQ=255)

                                             cGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGt                                                                                                   <  3:224627/149‑1 (MQ=255)

                                              gACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGt                                                                                                   <  3:216206/148‑1 (MQ=255)

                                              gACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGt                                                                                                   <  3:209239/148‑1 (MQ=255)

                                              gACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTc                                                                                                  >  5:177334/1‑149 (MQ=255)

                                               acacCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCg                                                                                                 <  4:371733/149‑1 (MQ=255)

                                                cacCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGc                                                                                                >  4:161778/1‑149 (MQ=255)

                                                 acCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGc                                                                                                       >  6:178413/1‑141 (MQ=255)

                                                 acCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGc                                                                                                       <  5:178413/141‑1 (MQ=255)

                                                  ccGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGcc                                                                                               >  6:198682/1‑148 (MQ=255)

                                                     gCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATc                                                                                           >  3:100833/1‑149 (MQ=255)

                                                         gcgcCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTAcc                                                                                       <  4:175794/149‑1 (MQ=255)

                                                         gcgcCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTAcc                                                                                       >  7:256164/1‑149 (MQ=255)

                                                         gcgcCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTAcc                                                                                       >  8:170147/1‑149 (MQ=255)

                                                           ggctggtgGTGCTGGCTGTATAGCTTGTCGCTTCGTTGCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTg                                                                                     <  3:212843/147‑1 (MQ=255)

                                                             ctgctgGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGc                                                                                   >  5:247414/1‑149 (MQ=255)

                                                                 tggtgctggCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGa                                                                                                                                     <  5:248149/96‑1 (MQ=255)

                                                                 tggtgctggCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGa                                                                                                                                     >  6:248149/1‑96 (MQ=255)

                                                                  ggtgctggCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACt                                                                              <  6:151461/149‑1 (MQ=255)

                                                                     gctggctgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACg                                                                           >  1:302829/1‑149 (MQ=255)

                                                                         gctgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGATTACGTc                                                                         <  2:364999/147‑1 (MQ=255)

                                                                         gctgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTc                                                                         >  7:326748/1‑147 (MQ=255)

                                                                          ctgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAg                                                                      <  8:281925/149‑1 (MQ=255)

                                                                          ctgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAg                                                                      <  4:203168/149‑1 (MQ=255)

                                                                           tgAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGc                                                                     <  5:175261/149‑1 (MQ=255)

                                                                             aaGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTg                                                                   <  1:129122/149‑1 (MQ=255)

                                                                              agagCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTgg                                                                  <  6:247414/149‑1 (MQ=255)

                                                                              agagCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTgg                                                                  <  6:177334/149‑1 (MQ=255)

                                                                              agagCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTg                                                                   >  8:4137/1‑148 (MQ=255)

                                                                              agagCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTg                                                                   <  7:4137/148‑1 (MQ=255)

                                                                               gagCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTAGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTggg                                                                 >  3:218085/1‑149 (MQ=255)

                                                                                agCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGa                                                                                <  4:120855/133‑1 (MQ=255)

                                                                                agCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGa                                                                                >  3:120855/1‑133 (MQ=255)

                                                                                  cTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGgcgc                                                              <  6:371515/149‑1 (MQ=255)

                                                                                    ggTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCg                                                            >  8:320999/1‑149 (MQ=255)

                                                                                     gtcgagtcgaGCCTGAAGCGCTACAACCTTGAACGTACGGTTCTTGCCAGAGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCCTTCTACGATCGACTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCgg                                                           >  2:340691/1‑149 (MQ=255)

                                                                                         aGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCAACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTAcc                                                       <  2:61179/149‑1 (MQ=255)

                                                                                              tGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCAAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgt                                                  <  6:953/149‑1 (MQ=255)

                                                                                                ccTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgtg                                                 <  5:52693/148‑1 (MQ=255)

                                                                                                ccTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgtg                                                 <  7:170147/148‑1 (MQ=255)

                                                                                                ccTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgccc                                                <  7:330072/149‑4 (MQ=255)

                                                                                                 cTGAAGCGCTACAACCTGGAAGGTTCGGGTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGGCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgtggt                                               <  6:221880/149‑1 (MQ=255)

                                                                                                   gAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCgtggtg                                              >  2:404262/1‑148 (MQ=255)

                                                                                                       cgcTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTc                                         >  2:353480/1‑149 (MQ=255)

                                                                                                        gcTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTcc                                        <  5:92304/149‑1 (MQ=255)

                                                                                                              aCCTGGAAGGTTCGGTTGTTGCAAGCGCCCAAGGTTAGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCAGGTACCGGCGAGGTGCATTCCTCga                                    >  6:452/1‑146 (MQ=255)

                                                                                                               ccTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGATCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTAAGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCAGGTAACGGCCTGCTGCATTCCTCGCAAg                                 >  7:184602/1‑149 (MQ=255)

                                                                                                                cTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCg                                                            <  8:224759/121‑1 (MQ=255)

                                                                                                                cTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCg                                                            >  7:224759/1‑121 (MQ=255)

                                                                                                                       gTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGt                         <  2:302829/149‑1 (MQ=255)

                                                                                                                        ttCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGt                         >  3:197863/1‑148 (MQ=255)

                                                                                                                           gggtgttgCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGGGGTGCATTCCTCGCCAGCCTACGGTGaa                      <  4:391736/145‑1 (MQ=255)

                                                                                                                            gttgttGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAga                    >  7:21432/1‑149 (MQ=255)

                                                                                                                            gttgttGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAga                    >  8:1085/1‑149 (MQ=255)

                                                                                                                             ttgttgCCAGCTCCCAAGGTTCGGCGCTGTAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGATCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACTGTGAAgac                   <  5:303882/149‑1 (MQ=255)

                                                                                                                                ttgCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAgccga                 >  8:174928/1‑148 (MQ=255)

                                                                                                                                ttgCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAgacga                 <  7:320999/148‑1 (MQ=255)

                                                                                                                                  tccAGCGCCGAACCTTGGGCGCTGGAGCTGACCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACtt              >  3:60059/2‑149 (MQ=255)

                                                                                                                                   ccAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTc             >  6:12087/1‑149 (MQ=255)

                                                                                                                                     aGCGCCCAAGGTTCGGCGCTGGAGCTTATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACt               >  1:166077/1‑145 (MQ=255)

                                                                                                                                     aGCGCCCAAGGTTCGGCGCTGGAGCTTATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACt               <  2:166077/145‑1 (MQ=255)

                                                                                                                                     aGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGt           <  1:404262/149‑1 (MQ=255)

                                                                                                                                      gcgcCGAACCTTGGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGt           >  2:704/1‑148 (MQ=255)

                                                                                                                                          ccAAGGTTCGGCGCTGGAGCTGATCAACTTTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCAcc       >  1:259507/1‑149 (MQ=255)

                                                                                                                                            aaGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACCTGc    <  8:21432/149‑1 (MQ=255)

                                                                                                                                              ggTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACCTGc    >  5:29973/1‑147 (MQ=255)

                                                                                                                                              ggTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACCTGCaa  <  3:400439/149‑1 (MQ=255)

CAACCAGGCTCTGAACATCCACCCGGAATTCAAGTACGCCCTGGTCGACACCGGCGAGCGCCTGCTGGTGCTGGCTGAAGAGCTGGTCGAGTCGTGCCTGAAGCGCTACAACCTGGAAGGTTCGGTTGTTGCCAGCGCCCAAGGTTCGGCGCTGGAGCTGATCAAC‑TTCCGTCATCCGTTCTACGATCGCCTGTCGCCAATCTACCTGGCCGACTACGTCGAGCTGGGCGCCGGTACCGGCGTGGTGCATTCCTCGCCAGCCTACGGTGAAGACGACTTCGTCACCTGCAA  >  NC_002947/710940‑711230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`