Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 791,454 T→A 5.6% H532L (CAC→CTC)  PP_0678 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947791,4540TA 5.6% 210.9 / 2.5 71H532L (CAC→CTC) PP_0678hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/36);  new base A (2/2);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

GCTGGTTCAGGCTTACACTGCGCCGCACTTCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATGCTTGCCCGGCACCCGCTCCAGCCCGCCGGCCACTTGC  >  NC_002947/791308‑791600
                                                                                                                                                  |                                                                                                                                                  
gctgGTTCAGGCTTACACTGCGCCGCACTTCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATTTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGa                                                                                                                                                  >  5:347145/1‑149 (MQ=255)
 ctgGTTCAGGCTTACACTGCGCCGCACTTCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTtgtg                                                                                                                                                   <  3:422166/147‑1 (MQ=255)
          ggttACACTGCGCCGCACTTCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCAAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGATGTCGTTGTGATGCTCGAccc                                                                                                                                        >  5:280698/3‑149 (MQ=255)
          gCTTACACTGCGCCGCACTTCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGAccc                                                                                                                                        <  3:16229/149‑1 (MQ=255)
            ttACACTGCGCCGCACTTCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCg                                                                                                                                       >  5:208743/1‑148 (MQ=255)
                  tGCGCCGCACTTCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGAc                                                                                                                                          <  1:237774/139‑1 (MQ=255)
                  tGCGCCGCACTTCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGAc                                                                                                                                          >  2:237774/1‑139 (MQ=255)
                  tGCGCCGCACTTCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTg                                                                                                                                >  6:21188/1‑149 (MQ=255)
                    cgccgcACTTCGCCGTTGGTGAAGTAATCGTCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCAATGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGcc                                                                                                                              >  8:220722/1‑149 (MQ=255)
                    cgccgcACTTCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTg                                                                                                                                <  6:386469/147‑1 (MQ=255)
                       cgcACTTCGCCGTTGGCGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGccc                                                                                                                             >  6:213991/1‑147 (MQ=255)
                        ggaCTTCGCCGTTGGTGAAGTAATCTCGCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCTCCTCAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAg                                                                                                                          <  8:347207/147‑1 (MQ=255)
                         cACTTCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGNTGTGATGCTCGACCCGCACCGTGCCCAAgg                                                                                                                         <  1:41891/149‑1 (MQ=255)
                          aCTTCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGc                                                                                                                        <  4:140193/149‑1 (MQ=255)
                            ttagCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGAGATGCTCGACCCGCACCGTGCCCAAGGCa                                                                                                                       >  7:161847/4‑148 (MQ=255)
                            ttCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCa                                                                                                                       >  7:355937/1‑148 (MQ=255)
                            ttCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCa                                                                                                                       >  6:42470/1‑148 (MQ=255)
                              cGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCaaa                                                                                                                     <  3:184807/148‑1 (MQ=255)
                              cGCCGATGGTGAAGTAATCGCCCTTGGACTCCAGCGTCATGTTGCCCAGCCCGTTGGGCCTCAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCaacc                                                                                                                    <  5:258853/149‑3 (MQ=255)
                               gCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAAc                                                                                                                    <  8:126709/148‑1 (MQ=255)
                               gCCGTTGGTGAAGAAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTTAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCaacc                                                                                                                    <  1:45528/148‑3 (MQ=255)
                                 cGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAAcccc                                                                                                                 <  5:21188/149‑1 (MQ=255)
                                  gTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCa                                                                                                                >  6:399830/1‑149 (MQ=255)
                                     ggTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGTGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGc                                                                                                              <  3:23751/148‑1 (MQ=255)
                                            tAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTc                                                                                                      <  8:355937/149‑1 (MQ=255)
                                             aaTCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTACCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAAGCAAACCCCAGCAAACCGTCg                                                                                                     >  8:377924/1‑149 (MQ=255)
                                              aTCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCg                                                                                                     <  6:175257/148‑1 (MQ=255)
                                                   ccTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGc                                                                                               >  5:192273/1‑149 (MQ=255)
                                                   ccTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGc                                                                                               >  5:314964/1‑149 (MQ=255)
                                                   ccTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGc                                                                                               >  7:192035/1‑149 (MQ=255)
                                                    cTTGGCCACCAGCGACAAGATGCCCATCCCGTTGCGCCACAGCAGCCCGCCAATGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTCTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCt                                                                                              <  6:280698/149‑1 (MQ=255)
                                                      tGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAgg                                                                                                                         <  4:164785/120‑1 (MQ=255)
                                                      tGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAgg                                                                                                                         >  3:164785/1‑120 (MQ=255)
                                                           tCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGCGCGAAGTAGTGGAGATGCTCGACCCGCCCCGTGCGGAAGGCAAACCCCAGCAAACCGTCGGCCAGCAGCCCGGt                                                                                       >  6:302131/1‑149 (MQ=255)
                                                           tCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGt                                                                                       >  4:150666/1‑149 (MQ=255)
                                                              aGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTgcc                                                                                    <  5:90004/149‑1 (MQ=255)
                                                              aGCGTCATGTTGCCCACCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTgcc                                                                                    <  8:66716/149‑1 (MQ=255)
                                                               gCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTgccg                                                                                   >  2:37901/1‑149 (MQ=255)
                                                                  tCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCa                                                                                <  1:26681/149‑1 (MQ=255)
                                                                         gCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGcgccgc                                                                         >  5:295708/1‑149 (MQ=255)
                                                                         gCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGcgccg                                                                          <  7:160401/148‑1 (MQ=255)
                                                                          cccAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGc                                                                                                                        <  6:415828/101‑1 (MQ=255)
                                                                          cccAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGc                                                                                                                        >  5:415828/1‑101 (MQ=255)
                                                                          cccAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGCGCAATGAACTGAGCGAAGTCGTTGAGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGcgccgc                                                                         <  5:302131/148‑1 (MQ=255)
                                                                           ccAGCCCGTTGCGCCACAGCCGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCa                                                                       <  8:357868/149‑1 (MQ=255)
                                                                            cAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCAt                                                                      <  6:314964/149‑1 (MQ=255)
                                                                             aGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCAtt                                                                     <  3:351845/149‑1 (MQ=255)
                                                                               cccGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTca                                                                   <  6:192273/149‑1 (MQ=255)
                                                                                 cGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTcaca                                                                 <  3:95981/149‑1 (MQ=255)
                                                                                 cGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTcac                                                                  >  6:181751/1‑148 (MQ=255)
                                                                                  gTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACAt                                                                <  7:220722/149‑1 (MQ=255)
                                                                                  gTTGCGCCACAGCAGCCCGCCATTGAAGACAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCTACCCGCACCGTGCCCAAGGCAAACCCCATCAAACCGTCGGGCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACAt                                                                <  6:347145/149‑1 (MQ=255)
                                                                                   ttGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTTGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATg                                                               >  7:21904/1‑149 (MQ=255)
                                                                                      cgcCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCt                                                            <  5:213991/149‑1 (MQ=255)
                                                                                      cgcCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCt                                                            <  6:295708/149‑1 (MQ=255)
                                                                                      cgcCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCt                                                            <  5:181751/149‑1 (MQ=255)
                                                                                         cacaGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCAc                                                         <  6:289709/149‑1 (MQ=255)
                                                                                         cacaGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCAc                                                         <  6:208743/149‑1 (MQ=255)
                                                                                         cacaGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGGCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCAc                                                         <  7:313515/149‑1 (MQ=255)
                                                                                                 cccGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACtt                                                 >  5:233935/1‑149 (MQ=255)
                                                                                                      cATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGAGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGtca                                            <  8:161847/149‑1 (MQ=255)
                                                                                                           aaGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATg                                       >  2:222905/1‑149 (MQ=255)
                                                                                                            aGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATGc                                      >  4:105192/1‑149 (MQ=255)
                                                                                                                        gggCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCg                                                                                                     >  7:11515/1‑74 (MQ=255)
                                                                                                                        gggCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCg                                                                                                     <  8:11515/74‑1 (MQ=255)
                                                                                                                            aaTGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATGCTTGCCCGGCACCCGCt                      >  2:46771/1‑149 (MQ=255)
                                                                                                                                 aCTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATGCTTGCCCGGCACCCGCTCCAg                  <  1:222905/148‑1 (MQ=255)
                                                                                                                                  cTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGAGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCACGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATGCTTGCCCGGCACCCGCTCCAGcc                >  1:263418/1‑149 (MQ=255)
                                                                                                                                    gAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATGCTTGCCCGGCACCCGCTCCAGCccg              >  5:317726/1‑149 (MQ=255)
                                                                                                                                            tCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATGCTTGCCCGGCACCCGCTCCAGCCCGCCGGCCAc      >  4:182211/1‑149 (MQ=255)
                                                                                                                                              gTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATGCTTGCCCGGCACCCGCTCCAGCCCGCCGGCCACtt    >  3:84042/1‑149 (MQ=255)
                                                                                                                                              gTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATGCTTGCCCGGCACCCGCTCCAGCCCGCCGGCCACtt    >  4:150745/1‑149 (MQ=255)
                                                                                                                                                 gtgATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATGCTTGCCCGGCACCCGCTCCAGCCCGCCGGCCACTTGc  >  8:194978/1‑148 (MQ=255)
                                                                                                                                                 gtgATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATGCTTGCCCGGCACCCGCTCCAGCCCGCCGGCCACTTGc  >  6:54762/1‑148 (MQ=255)
                                                                                                                                                 gtgATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATGCTTGCCCGGCACCCGCTCCAGCCCGCCGGCCACTTGc  >  6:48378/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCTGGTTCAGGCTTACACTGCGCCGCACTTCGCCGTTGGTGAAGTAATCGCCCTTGGCCTCCAGCGTCATGTTGCCCAGCCCGTTGCGCCACAGCAGCCCGCCATTGAAGCCAGCTGCCGGGGCAATGAACTGAGCGAAGTCGTTGTGATGCTCGACCCGCACCGTGCCCAAGGCAAACCCCAGCAAACCGTCGGCCAGCTGCCAGGTGCCGCCAGCGCCGCCATTCACATGGCTCACCAGCACTTCGTCATCATGCTTGCCCGGCACCCGCTCCAGCCCGCCGGCCACTTGC  >  NC_002947/791308‑791600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑