Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 813,200 T→G 9.4% intergenic (+107/+24) PP_0699 → / ← PP_t70 LysE family transporter/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947813,2000TG 9.4% 183.2 / 10.3 64intergenic (+107/+24)PP_0699/PP_t70LysE family transporter/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base T (30/28);  new base G (4/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

CGGGCTTGGCCTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATT  >  NC_002947/813054‑813347
                                                                                                                                                  |                                                                                                                                                   
cGGGCTTGGCCTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTg                                                                                                                                                   >  7:188320/1‑149 (MQ=255)
  ggCTTGGCCTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCg                                                                                                                                                 <  5:52804/149‑1 (MQ=255)
         ccTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGcc                                                                                                                                          >  6:380400/1‑149 (MQ=255)
          cTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGccc                                                                                                                                         <  1:239841/149‑1 (MQ=255)
                   gCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTg                                                                                                                                >  3:272105/1‑149 (MQ=255)
                         aCCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtggt                                                                                                                          <  5:380400/149‑1 (MQ=255)
                         aCCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtgg                                                                                                                           <  2:91051/148‑1 (MQ=255)
                          ccGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtggt                                                                                                                          <  4:272105/148‑1 (MQ=255)
                           cGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtggtgg                                                                                                                        <  8:188320/149‑1 (MQ=255)
                           cGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtggtgg                                                                                                                        >  8:124305/1‑149 (MQ=255)
                           cGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtggtgg                                                                                                                        <  3:359898/149‑1 (MQ=255)
                           cGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCGCAAGGTAAGCCCttt                                                                                                                                      >  5:61031/1‑135 (MQ=255)
                           cGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCGCAAGGTAAGCCCttt                                                                                                                                      <  6:61031/135‑1 (MQ=255)
                               aTACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTac                                                                                                                    <  7:124305/149‑1 (MQ=255)
                                tACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtggtg                                                                                                                         <  2:432854/143‑1 (MQ=255)
                                tACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTtggtg                                                                                                                         >  1:432854/1‑143 (MQ=255)
                                  cGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTAcaca                                                                                                                 <  2:342702/149‑1 (MQ=255)
                                     tGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAgg                                                                                                               >  8:103315/1‑148 (MQ=255)
                                        ggCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACt                                                                                                           <  3:222388/149‑1 (MQ=255)
                                         gCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACtt                                                                                                          >  4:346242/1‑149 (MQ=255)
                                           gacgaTAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTATTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGCCTTg                                                                                                         >  1:412255/1‑148 (MQ=255)
                                                tAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAAcccc                                                                                                   >  5:155382/1‑149 (MQ=255)
                                                   cagcGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGa                                                                                                <  7:103315/149‑1 (MQ=255)
                                                        ccGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCa                                                                                           <  1:199267/149‑1 (MQ=255)
                                                           aaaCCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCa                                                                                        <  5:27594/149‑1 (MQ=255)
                                                              ccTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTa                                                                                     >  8:232349/1‑149 (MQ=255)
                                                              ccTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTa                                                                                     <  6:380941/149‑1 (MQ=255)
                                                                tgtgATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTAt                                                                                    >  2:33483/1‑148 (MQ=255)
                                                                 gtgATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATg                                                                                   >  8:324165/1‑148 (MQ=255)
                                                                 gtgATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATg                                                                                   <  7:324165/148‑1 (MQ=255)
                                                                   aaTTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAat                                                                                >  4:162406/2‑149 (MQ=255)
                                                                    aTTCGGCGCCCGGCTGCTGCGGGATTGGTGGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAat                                                                                <  5:228606/148‑1 (MQ=255)
                                                                     ttCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGAGAc                                                                                                            <  5:247014/119‑1 (MQ=255)
                                                                     ttCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGAGAc                                                                                                            >  6:247014/1‑119 (MQ=255)
                                                                         gcgcCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAatgctatg                                                                          >  5:161236/1‑149 (MQ=255)
                                                                         gcgcCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAatgctatg                                                                          >  7:260612/1‑149 (MQ=255)
                                                                             ccGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGctct                                                                      <  7:212114/149‑1 (MQ=255)
                                                                                     tgcGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACt                                                              >  7:81973/1‑149 (MQ=255)
                                                                                      gcGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACt                                                              >  5:407725/1‑148 (MQ=255)
                                                                                       cGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACt                                                              <  6:155382/147‑1 (MQ=255)
                                                                                           aTTTGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTa                                                        <  1:370730/149‑1 (MQ=255)
                                                                                               gttggCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGAc                                                                                                            <  8:90938/93‑1 (MQ=255)
                                                                                               gttggCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACt                                                              <  8:296009/139‑1 (MQ=255)
                                                                                               gttggCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACACCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACt                                                              >  7:296009/1‑139 (MQ=255)
                                                                                                ttggCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGACTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAg                                                   >  8:7004/1‑149 (MQ=255)
                                                                                                         gCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGgcg                                          >  2:342966/1‑149 (MQ=255)
                                                                                                                    tttttCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATtcg                               >  4:245344/1‑148 (MQ=255)
                                                                                                                       ttCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTctct                             <  6:407725/148‑1 (MQ=255)
                                                                                                                             aaacaaGACAAAGGGCTTACCGCAAGGTAAGCCCTTTTTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGc                      >  8:312385/5‑149 (MQ=38)
                                                                                                                              gCAAAGAAAAAGGGCTTACCTTGCGGTAAGCGCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCGATGTAGCCGATGGCGCGCAATATTCTCTTGAACGcc                     >  2:249349/1‑149 (MQ=255)
                                                                                                                                aaaGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGACCCCCGGACCCCAGCATTATGAATGGTATGCTCTAACCAACTGAGCTATTTAGCCGCTGGCGCGCATTATTCTCTTGAACGcccc                   >  3:308500/1‑149 (MQ=255)
                                                                                                                                aaaGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGAGCGCATTATTCGCTTGACCGcccc                   >  7:120089/1‑149 (MQ=255)
                                                                                                                                aaaGAAAAAGGGCTTACATTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGAACCCAGCATTATGAATGCTATGCTCTAACCAAATGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGcccc                   >  8:376491/1‑149 (MQ=255)
                                                                                                                                  aGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCct                 <  3:346242/149‑1 (MQ=255)
                                                                                                                                   gacaaaGGGCTTACCGCAAGGTAAGCCCTTTTTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGa                                               >  7:376762/4‑118 (MQ=38)
                                                                                                                                   gacaaaGGGCTTACCGCAAGGTAAGCCCTTTTTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGa                                               <  8:376762/115‑1 (MQ=38)
                                                                                                                                      aaaGGGCTTACCGCAAGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGt             >  8:348558/1‑149 (MQ=255)
                                                                                                                                        aGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTc            >  1:235098/1‑148 (MQ=255)
                                                                                                                                          ggCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCTTCTGTCAac         <  8:81973/149‑1 (MQ=255)
                                                                                                                                           gCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAaca        >  6:213810/1‑149 (MQ=255)
                                                                                                                                            cTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCtt                            <  1:187861/128‑1 (MQ=255)
                                                                                                                                            cTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCtt                            >  2:187861/1‑128 (MQ=255)
                                                                                                                                            cTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAacac       >  3:66419/1‑149 (MQ=255)
                                                                                                                                                 cTTGCGGTAAGCCCTTTGTCTTGTTTGGTGACTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCAtt  <  7:7004/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGGGCTTGGCCTGGCTGTGGCGGTGACCGGAATACGTTGAGGCGACGATAGCAGCGCCGAAACCTGTGATTCGGCGCCCGGCTGCTGCGGGATTGGTTGGCGCGGGCATCAGCAACTTTTTCGCAGGCAAAGAAAAAGGGCTTACCTTGCGGTAAGCCCTTTGTCTTGTTTGGTGGCTACACAGGGACTTGAACCCCGGACCCCAGCATTATGAATGCTATGCTCTAACCAACTGAGCTATGTAGCCGATGGCGCGCATTATTCTCTTGAACGCCCCCTCTGTCAACACTCATT  >  NC_002947/813054‑813347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑