BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	848,503	G→A	5.3%	L23F (CTT→TTT)	PP_0730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	848,503	0	G	A	5.3%	351.0 / 4.7	113	L23F (CTT→TTT)	PP_0730	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (48/59); new base A (3/3); total (51/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01

Only 100 of 113 total aligned reads displayed.

GGCTGAATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAG  >  NC_002947/848356‑848646

ggCTGAATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGc                                                                                                                                                >  6:220783/1‑149 (MQ=255)

 gCTGAATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGcc                                                                                                                                               >  7:378460/1‑149 (MQ=255)

     aaTTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGttt                                                                                                                                           >  4:114931/1‑149 (MQ=255)

     aaTTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGttt                                                                                                                                           <  6:140220/149‑1 (MQ=255)

     aaTTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGtt                                                                                                                                            <  7:18334/148‑1 (MQ=255)

     aaTTCGCCGCCTGCCAGATTATCGATACGCAAATAAGCGTCCAGATATCGCAGGTGAGGCGCGGGAGGGGAGGTGTGGTCCTGGATCGAGATGGTCAGGGCGGAGGCGGGGCGCGGATGAAGGAGGGGGACGTGGCAGCCAGGCCGtgt                                                                                                                                           >  4:251433/1‑147 (MQ=255)

      aTTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGttt                                                                                                                                           <  2:300954/148‑1 (MQ=255)

         cgccgcCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCATGATCGAGATCGTCAGGAACGAGCCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCt                                                                                                                                       >  1:56374/1‑149 (MQ=255)

          gccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGCGGCGTCGCGCTGATGAATGATGGTGACGTGGCAGCCTAGCCGTTTGGCTg                                                                                                                                      >  6:307410/1‑149 (MQ=255)

           ccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGc                                                                                                                                     >  6:266747/1‑149 (MQ=255)

           ccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGc                                                                                                                                     <  6:410309/149‑1 (MQ=255)

           ccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGc                                                                                                                                     <  2:231944/149‑1 (MQ=255)

           ccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGc                                                                                                                                     <  8:378460/149‑1 (MQ=255)

           ccgccTTCCAGATTATCGAAACGCAAATAAGCATCCACATAACGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGCAACGAGGCGTCGCGCTGATGAATGAAGATGACGTCGCCGCCAAGCAGTTTGGCTGc                                                                                                                                     >  4:7377/1‑149 (MQ=255)

            cgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGcc                                                                                                                                    <  5:9294/149‑1 (MQ=255)

            cgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGcc                                                                                                                                    >  5:171699/1‑149 (MQ=255)

            cgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAACGGCTTGGCTGcc                                                                                                                                    <  2:100138/149‑1 (MQ=255)

            cgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGAACGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGcc                                                                                                                                    <  7:135301/149‑1 (MQ=255)

              ccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGAGGCAGCCAAACGGCTTGGCTGccac                                                                                                                                  <  6:68701/149‑3 (MQ=255)

                    aGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGc                                                                                                                            <  5:220783/149‑1 (MQ=255)

                    aGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGc                                                                                                                            >  3:177903/1‑149 (MQ=255)

                    aGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGAGTCGTCCTTGATCGAGATCGTCAGGAACGAGCCGTCGAGCTGATGAATGAAGTTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAga                                                                                                                            >  5:335990/1‑148 (MQ=255)

                       ttATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCcag                                                                                                                         <  6:171699/149‑1 (MQ=255)

                         aTCGATACTCAAATAAGCATCCAGATATCGCAGGTAAGGCTCGAGACGGTAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCcagc                                                                                                                        <  8:105292/148‑1 (MQ=255)

                              tACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCTGTTTGGCTGCCTCAAAAGCCAGCAGGCCg                                                                                                                  <  1:253597/149‑1 (MQ=255)

                              tACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCg                                                                                                                  >  3:128217/1‑149 (MQ=255)

                                cGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCga                                                                                                                 <  3:251433/148‑2 (MQ=255)

                                   aaaTAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGc                                                                                                                    <  8:377389/142‑1 (MQ=255)

                                   aaaTAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTAc                                                                                                             >  1:301074/1‑149 (MQ=255)

                                          cATACACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGGCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTg                                                                                                      <  8:341244/149‑1 (MQ=255)

                                           aTCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGt                                                                                                     >  6:128610/1‑149 (MQ=255)

                                             ccACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTc                                                                                                    >  6:147626/1‑148 (MQ=255)

                                               acaTATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTcgac                                                                                                 >  8:192667/1‑149 (MQ=255)

                                                   atCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGaa                                                                                              >  6:379657/1‑148 (MQ=255)

                                                   atCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGaa                                                                                              >  4:72006/1‑148 (MQ=255)

                                                        aGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCAc                                                                                        <  5:190980/149‑1 (MQ=255)

                                                          gTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTcga                                                                                                  >  1:58084/1‑137 (MQ=255)

                                                          gTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTcga                                                                                                  <  2:58084/137‑1 (MQ=255)

                                                          gTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGAAGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGCCGAAAACCACgt                                                                                      >  2:111179/1‑149 (MQ=255)

                                                            aaGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAGGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACgtgt                                                                                    >  8:226114/1‑149 (MQ=255)

                                                            aaGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGACAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGACGCTTACGGTGCTGTCGACGAAAACCACgtgt                                                                                    >  1:78194/1‑149 (MQ=255)

                                                             aGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACgtgt                                                                                    <  2:402442/148‑1 (MQ=255)

                                                               gCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGttt                                                                                  >  5:217407/1‑148 (MQ=255)

                                                                cTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCa                                                                                <  5:142378/149‑1 (MQ=255)

                                                                cTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCa                                                                                >  5:431840/1‑149 (MQ=255)

                                                                   gagaCGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCAtt                                                                              <  3:255935/148‑1 (MQ=255)

                                                                   gagaCGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTg                                                                             >  1:186472/1‑149 (MQ=255)

                                                                       cGGGAGGGGTCGTCCTTGATCTTGATCGGCAGGAACGAGGCGTCGAGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCATCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAAc                                                                         <  8:151369/149‑1 (MQ=255)

                                                                          gAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTc                                                                      <  3:72006/149‑1 (MQ=255)

                                                                          gAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTc                                                                      <  5:56278/149‑1 (MQ=255)

                                                                           aGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCg                                                                     <  2:301074/149‑1 (MQ=255)

                                                                            ggTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCgg                                                                    <  5:266747/149‑1 (MQ=255)

                                                                             gtgtCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGa                                                                   >  1:105829/1‑149 (MQ=255)

                                                                             gtgtCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGa                                                                   <  5:128610/149‑1 (MQ=255)

                                                                                   tcCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTc                                                                                 >  2:350489/1‑129 (MQ=255)

                                                                                   tcCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTc                                                                                 <  1:350489/129‑1 (MQ=255)

                                                                                     cTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAgg                                                           >  4:271526/1‑149 (MQ=255)

                                                                                     cTTGATCGACATCGTCACGATCGAGGGGGCGCGCTGATGAAGGATCGTGACGCGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAAGCACGTGTTTCATTGGAACCTCGGATTACCAgg                                                           <  4:100198/149‑1 (MQ=255)

                                                                                       tGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGa                                                         >  8:334225/1‑149 (MQ=255)

                                                                                        gATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTAc                                                               <  7:203778/142‑1 (MQ=255)

                                                                                        gATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTAc                                                               >  8:203778/1‑142 (MQ=255)

                                                                                        gATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGa                                                         <  4:128217/148‑1 (MQ=255)

                                                                                        gATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGAt                                                        >  5:338684/1‑149 (MQ=255)

                                                                                            gagaTCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGaaa                                                    <  2:186472/149‑1 (MQ=255)

                                                                                            gagaTCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGaaa                                                    <  3:159982/149‑1 (MQ=255)

                                                                                                     aGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGt                                           <  5:147626/149‑1 (MQ=255)

                                                                                                       gAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCAt                                                                               <  8:317302/111‑1 (MQ=255)

                                                                                                       gAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCAt                                                                               >  7:317302/1‑111 (MQ=255)

                                                                                                        aaCGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCATCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGc                                         <  1:226590/148‑1 (MQ=255)

                                                                                                         aCGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTg                                       <  3:88884/149‑1 (MQ=255)

                                                                                                         aCGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTg                                       <  6:372148/149‑1 (MQ=255)

                                                                                                          cGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGa                                      <  3:271526/149‑1 (MQ=255)

                                                                                                               cGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCg                                 <  1:202712/149‑1 (MQ=255)

                                                                                                                gTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGGGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCg                                 >  8:330580/1‑148 (MQ=255)

                                                                                                                   gcgcTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGaa                               >  3:363911/1‑147 (MQ=255)

                                                                                                                    cgcTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAACCCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAg                            >  6:417468/1‑149 (MQ=255)

                                                                                                                         atgtatgaAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTg                       >  7:154263/1‑149 (MQ=255)

                                                                                                                         atgaatgaAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTg                       >  1:5982/1‑149 (MQ=255)

                                                                                                                          tgaatgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTg                       <  7:184172/148‑1 (MQ=255)

                                                                                                                           gaatgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGc                      >  2:91128/1‑148 (MQ=255)

                                                                                                                             atgaaGTTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGGAGGGCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCTGGGATGAAAGTAAATAGTGCTGTGCCCGAACATCATTGCGa                    <  6:335990/148‑1 (MQ=255)

                                                                                                                             atgaaGGTGACTTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                   <  4:177903/149‑1 (MQ=255)

                                                                                                                             atgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                   <  7:192667/149‑1 (MQ=255)

                                                                                                                             atgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                   <  6:338684/149‑1 (MQ=255)

                                                                                                                             atgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                   <  2:105829/149‑1 (MQ=255)

                                                                                                                              tgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaag                  <  6:217407/149‑1 (MQ=255)

                                                                                                                              tgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaag                  >  8:153018/1‑149 (MQ=255)

                                                                                                                               gaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                   <  5:365342/147‑1 (MQ=255)

                                                                                                                                   gTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGACCGAACAGCATTGCGaagcaagc             >  7:163516/1‑149 (MQ=255)

                                                                                                                                     tttgaggCAGCCAAACGGCTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaagcaagc             <  8:143385/142‑1 (MQ=255)

                                                                                                                                     gACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGaa                               <  6:196786/129‑1 (MQ=255)

                                                                                                                                      aCGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGTGAc          <  7:58859/149‑1 (MQ=255)

                                                                                                                                         tGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGt       <  4:363911/149‑1 (MQ=255)

                                                                                                                                          ggCAGCCAAACGGCTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGATTCAAGCGCCCGTc      >  1:152093/1‑149 (MQ=255)

                                                                                                                                             aGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGaa                               <  6:24076/121‑1 (MQ=255)

                                                                                                                                             aGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGaa                               >  5:24076/1‑121 (MQ=255)

                                                                                                                                             aGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaag                  <  6:69583/134‑1 (MQ=255)

                                                                                                                                             aGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaag                  >  5:69583/1‑134 (MQ=255)

                                                                                                                                              gCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAg  <  7:334225/149‑1 (MQ=255)

                                                                                                                                              gCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAg  <  2:5982/149‑1 (MQ=255)

GGCTGAATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAG  >  NC_002947/848356‑848646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`