BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	848,505	C→G	5.4%	R22P (CGG→CCG)	PP_0730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	848,505	0	C	G	5.4%	353.7 / 7.8	111	R22P (CGG→CCG)	PP_0730	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (45/58); new base G (3/3); total (49/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.39e-01

Only 100 of 114 total aligned reads displayed.

GCTGAATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAGGTGCGCC  >  NC_002947/848357‑848653

    aaTTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGttt                                                                                                                                                  <  6:140220/149‑1 (MQ=255)

    aaTTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGtt                                                                                                                                                   <  7:18334/148‑1 (MQ=255)

    aaTTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGt                                                                                                                                                    <  3:119618/147‑1 (MQ=255)

    aaTTCGCCGCCTGCCAGATTATCGATACGCAAATAAGCGTCCAGATATCGCAGGTGAGGCGCGGGAGGGGAGGTGTGGTCCTGGATCGAGATGGTCAGGGCGGAGGCGGGGCGCGGATGAAGGAGGGGGACGTGGCAGCCAGGCCGtgt                                                                                                                                                  >  4:251433/1‑147 (MQ=255)

     aTTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGttt                                                                                                                                                  <  2:300954/148‑1 (MQ=255)

       tCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGc                                                                                                                                               >  6:190980/1‑149 (MQ=255)

        cgccgcCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCATGATCGAGATCGTCAGGAACGAGCCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCt                                                                                                                                              >  1:56374/1‑149 (MQ=255)

         gccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGCGGCGTCGCGCTGATGAATGATGGTGACGTGGCAGCCTAGCCGTTTGGCTg                                                                                                                                             >  6:307410/1‑149 (MQ=255)

         gccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTg                                                                                                                                             <  5:422084/149‑1 (MQ=255)

          ccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGc                                                                                                                                            <  2:231944/149‑1 (MQ=255)

          ccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGc                                                                                                                                            >  6:266747/1‑149 (MQ=255)

          ccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGc                                                                                                                                            <  6:410309/149‑1 (MQ=255)

          ccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGc                                                                                                                                            <  8:378460/149‑1 (MQ=255)

           cgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGcc                                                                                                                                           >  5:171699/1‑149 (MQ=255)

           cgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGcc                                                                                                                                           <  5:9294/149‑1 (MQ=255)

           cgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAACGGCTTGGCTGcc                                                                                                                                           <  2:100138/149‑1 (MQ=255)

           cgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGAACGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGcc                                                                                                                                           <  7:135301/149‑1 (MQ=255)

             ccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGAGGCAGCCAAACGGCTTGGCTGccac                                                                                                                                         <  6:68701/149‑3 (MQ=255)

                   aGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGc                                                                                                                                   >  3:177903/1‑149 (MQ=255)

                   aGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGAGTCGTCCTTGATCGAGATCGTCAGGAACGAGCCGTCGAGCTGATGAATGAAGTTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAga                                                                                                                                   >  5:335990/1‑148 (MQ=255)

                      ttATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCcag                                                                                                                                <  6:171699/149‑1 (MQ=255)

                        aTCGATACTCAAATAAGCATCCAGATATCGCAGGTAAGGCTCGAGACGGTAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCcagc                                                                                                                               <  8:105292/148‑1 (MQ=255)

                             tACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCTGTTTGGCTGCCTCAAAAGCCAGCAGGCCg                                                                                                                         <  1:253597/149‑1 (MQ=255)

                             tACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCg                                                                                                                         >  3:128217/1‑149 (MQ=255)

                                  aaaTAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGc                                                                                                                           <  8:377389/142‑1 (MQ=255)

                                  aaaTAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGc                                                                                                                           >  7:377389/1‑142 (MQ=255)

                                  aaaTAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTAc                                                                                                                    >  1:301074/1‑149 (MQ=255)

                                  aaaTAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTAc                                                                                                                    <  2:206355/149‑1 (MQ=255)

                                         cATACACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGGCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTg                                                                                                             <  8:341244/149‑1 (MQ=255)

                                          aTCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGt                                                                                                            >  6:128610/1‑149 (MQ=255)

                                            ccACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTc                                                                                                           >  6:147626/1‑148 (MQ=255)

                                              acaTATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTcgac                                                                                                        >  8:192667/1‑149 (MQ=255)

                                                  atCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGaa                                                                                                     >  4:72006/1‑148 (MQ=255)

                                                       aGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCAc                                                                                               <  5:190980/149‑1 (MQ=255)

                                                         gTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTcga                                                                                                         >  1:58084/1‑137 (MQ=255)

                                                         gTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGAAGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGCCGAAAACCACgt                                                                                             >  2:111179/1‑149 (MQ=255)

                                                           aaGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAGGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACgtgt                                                                                           >  8:226114/1‑149 (MQ=255)

                                                           aaGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGACAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGACGCTTACGGTGCTGTCGACGAAAACCACgtgt                                                                                           >  1:78194/1‑149 (MQ=255)

                                                            aGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACgtgt                                                                                           <  2:402442/148‑1 (MQ=255)

                                                              gCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGttt                                                                                         >  5:217407/1‑148 (MQ=255)

                                                               cTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCa                                                                                       >  5:431840/1‑149 (MQ=255)

                                                               cTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCa                                                                                       <  5:142378/149‑1 (MQ=255)

                                                                  gagaCGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCAtt                                                                                     <  3:255935/148‑1 (MQ=255)

                                                                  gagaCGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTg                                                                                    >  1:186472/1‑149 (MQ=255)

                                                                      cGGGAGGGGTCGTCCTTGATCTTGATCGGCAGGAACGAGGCGTCGAGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCATCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAAc                                                                                <  8:151369/149‑1 (MQ=255)

                                                                         gAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTc                                                                             <  5:56278/149‑1 (MQ=255)

                                                                         gAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTc                                                                             <  3:72006/149‑1 (MQ=255)

                                                                          aGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCg                                                                            <  2:301074/149‑1 (MQ=255)

                                                                           ggTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCgg                                                                           <  5:266747/149‑1 (MQ=255)

                                                                            gtgtCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGa                                                                          >  1:105829/1‑149 (MQ=255)

                                                                            gtgtCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGa                                                                          <  5:128610/149‑1 (MQ=255)

                                                                                  tcCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTc                                                                                        <  1:350489/129‑1 (MQ=255)

                                                                                  tcCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTc                                                                                        >  2:350489/1‑129 (MQ=255)

                                                                                    cTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAgg                                                                  >  4:271526/1‑149 (MQ=255)

                                                                                    cTTGATCGACATCGTCACGATCGAGGGGGCGCGCTGATGAAGGATCGTGACGCGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAAGCACGTGTTTCATTGGAACCTCGGATTACCAgg                                                                  <  4:100198/149‑1 (MQ=255)

                                                                                      tGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGa                                                                >  8:334225/1‑149 (MQ=255)

                                                                                       gATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTAc                                                                      >  8:203778/1‑142 (MQ=255)

                                                                                       gATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTAc                                                                      <  7:203778/142‑1 (MQ=255)

                                                                                       gATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGa                                                                <  4:128217/148‑1 (MQ=255)

                                                                                       gATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGAt                                                               >  5:338684/1‑149 (MQ=255)

                                                                                           gagaTCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGaaa                                                           <  3:159982/149‑1 (MQ=255)

                                                                                                    aGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGt                                                  <  5:147626/149‑1 (MQ=255)

                                                                                                      gAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCAt                                                                                      >  7:317302/1‑111 (MQ=255)

                                                                                                      gAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCAt                                                                                      <  8:317302/111‑1 (MQ=255)

                                                                                                       aaCGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCATCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGc                                                <  1:226590/148‑1 (MQ=255)

                                                                                                        aCGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTg                                              <  6:372148/149‑1 (MQ=255)

                                                                                                        aCGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTg                                              <  3:88884/149‑1 (MQ=255)

                                                                                                         cGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGa                                             <  3:271526/149‑1 (MQ=255)

                                                                                                              cGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCg                                        <  1:202712/149‑1 (MQ=255)

                                                                                                               gTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGGGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCg                                        >  8:330580/1‑148 (MQ=255)

                                                                                                                  gcgcTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGaa                                      >  3:363911/1‑147 (MQ=255)

                                                                                                                   cgcTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAACCCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAg                                   >  6:417468/1‑149 (MQ=255)

                                                                                                                      tgatgaATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCAt                                >  3:393801/1‑149 (MQ=255)

                                                                                                                        atgtatgaAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTg                              >  7:154263/1‑149 (MQ=255)

                                                                                                                        atgaatgaAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTg                              >  1:5982/1‑149 (MQ=255)

                                                                                                                         tgaatgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTg                              <  7:184172/148‑1 (MQ=255)

                                                                                                                          gaatgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGc                             >  2:91128/1‑148 (MQ=255)

                                                                                                                            atgaaGTTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGGAGGGCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCTGGGATGAAAGTAAATAGTGCTGTGCCCGAACATCATTGCGa                           <  6:335990/148‑1 (MQ=255)

                                                                                                                            atgaaGGTGACTTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                          <  4:177903/149‑1 (MQ=255)

                                                                                                                            atgaaGGTGACGTGGCATCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                          <  4:347518/149‑1 (MQ=255)

                                                                                                                            atgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                          <  2:105829/149‑1 (MQ=255)

                                                                                                                            atgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                          <  7:192667/149‑1 (MQ=255)

                                                                                                                            atgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                          <  6:338684/149‑1 (MQ=255)

                                                                                                                             tgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaag                         >  8:153018/1‑149 (MQ=255)

                                                                                                                              gaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                          >  6:365342/1‑147 (MQ=255)

                                                                                                                              gaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                          <  5:365342/147‑1 (MQ=255)

                                                                                                                                    tttgaggCAGCCAAACGGCTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaagcaagc                    >  7:143385/6‑147 (MQ=255)

                                                                                                                                    tttgaggCAGCCAAACGGCTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaagcaagc                    <  8:143385/142‑1 (MQ=255)

                                                                                                                                    gACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGaa                                      <  6:196786/129‑1 (MQ=255)

                                                                                                                                    gACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGaa                                      >  5:196786/1‑129 (MQ=255)

                                                                                                                                     aCGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGTGAc                 <  7:58859/149‑1 (MQ=255)

                                                                                                                                        tGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGt              <  4:363911/149‑1 (MQ=255)

                                                                                                                                         ggCAGCCAAACGGCTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGATTCAAGCGCCCGTc             >  1:152093/1‑149 (MQ=255)

                                                                                                                                            aGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGaa                                      <  6:24076/121‑1 (MQ=255)

                                                                                                                                            aGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGaa                                      >  5:24076/1‑121 (MQ=255)

                                                                                                                                            aGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaag                         <  6:69583/134‑1 (MQ=255)

                                                                                                                                             gCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAg         <  7:334225/149‑1 (MQ=255)

                                                                                                                                             gCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAg         <  2:5982/149‑1 (MQ=255)

                                                                                                                                                   ccGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTTAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAGGTgcgc   <  2:56374/149‑1 (MQ=255)

                                                                                                                                                    cGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAGGTGCTcc  <  2:78194/149‑1 (MQ=255)

GCTGAATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAGGTGCGCC  >  NC_002947/848357‑848653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`