Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 924,319 T→C 7.9% T292A (ACG→GCG)  PP_0804 ← protein secretion ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947924,3190TC 7.9% 277.4 / 10.1 101T292A (ACG→GCG) PP_0804protein secretion ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (44/48);  new base C (4/4);  total (48/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.34e-01

Only 100 of 103 total aligned reads displayed.

GGCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTG  >  NC_002947/924173‑924467
                                                                                                                                                  |                                                                                                                                                    
tgCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTc                                                                                                                                                     <  4:100821/147‑1 (MQ=255)
   cAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCgg                                                                                                                                                 >  7:149753/1‑149 (MQ=255)
     aCCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTg                                                                                                                                               >  3:421550/1‑149 (MQ=255)
     aCCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTg                                                                                                                                               <  4:184099/149‑1 (MQ=255)
        gTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTg                                                                                                                                            >  1:238160/1‑149 (MQ=255)
        gTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTg                                                                                                                                            >  4:191382/1‑149 (MQ=255)
           ccAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGCGACGGTGCTggc                                                                                                                                          <  4:32529/148‑2 (MQ=255)
            catgcgcCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACg                                                                                                                                        >  7:186392/4‑149 (MQ=255)
             aGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGt                                                                                                                                       >  3:52189/1‑149 (MQ=255)
               gcgcCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGa                                                                                                                                     <  5:140689/149‑1 (MQ=255)
                 gcCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATc                                                                                                                                   <  1:161882/149‑1 (MQ=255)
                 gcCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATc                                                                                                                                   <  2:294046/149‑1 (MQ=255)
                    tttGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAgg                                                                                                                                >  5:220591/1‑149 (MQ=255)
                        tgCAATCCACGCGGGCCTCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTcgc                                                                                                                            <  8:337058/149‑1 (MQ=255)
                        tgCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATAAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCCGGTcgc                                                                                                                            >  8:311597/1‑149 (MQ=255)
                         gcaagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTcgc                                                                                                                            <  8:149753/148‑1 (MQ=255)
                          caagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCgg                                                                                                                          <  1:190078/149‑1 (MQ=255)
                            agcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGAt                                                                                                                        <  2:368456/149‑1 (MQ=255)
                                 cccGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGTCCAGCACCGCGACGGTGCt                                                                                                                                             <  6:312762/123‑1 (MQ=255)
                                 cccGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGTCCAGCACCGCGACGGTGCt                                                                                                                                             >  5:312762/1‑123 (MQ=255)
                                    gggCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCa                                                                                                                <  3:163333/149‑1 (MQ=255)
                                     ggCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAg                                                                                                               <  2:245243/149‑1 (MQ=255)
                                            ccATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACg                                                                                                                                        <  7:233848/117‑1 (MQ=255)
                                            ccATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCgcgc                                                                                                        <  8:83542/149‑1 (MQ=255)
                                             cATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCgcgc                                                                                                        >  6:13725/1‑148 (MQ=255)
                                               tGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAAc                                                                                                     >  8:295219/1‑149 (MQ=255)
                                                   cagcagCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCg                                                                                                 <  6:162986/149‑1 (MQ=255)
                                                    agcagcGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCga                                                                                                <  7:22239/149‑1 (MQ=255)
                                                    agcagcGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCga                                                                                                <  2:238160/149‑1 (MQ=255)
                                                     gcagcGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGc                                                                                                  >  3:334522/1‑146 (MQ=255)
                                                       agcGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGAt                                                                                             <  3:386075/149‑1 (MQ=255)
                                                        gcgcGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGc                                                                                                  <  2:419896/143‑1 (MQ=255)
                                                        gcgcGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGc                                                                                                  >  1:419896/1‑143 (MQ=255)
                                                         cgcgACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGa                                                                                           <  3:89942/149‑1 (MQ=255)
                                                               cAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGc                                                                                     <  4:334522/149‑1 (MQ=255)
                                                                 gCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCACGTCGGCCAGCACCGTCGCGGTGCTGGACCTGATCAGGTCGCGGATCGACTCCAGATCGCGCAACTGCGAGATGAACGAGCCg                                                                                   >  3:304946/1‑149 (MQ=255)
                                                                  cGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCgg                                                                                  <  7:279718/149‑1 (MQ=255)
                                                                     gATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTg                                                                                >  5:321786/1‑148 (MQ=255)
                                                                        gAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACt                                                                            >  3:282502/1‑149 (MQ=255)
                                                                        gAACACCAGTACGCCGCCGATCAACCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACt                                                                            >  5:314421/1‑149 (MQ=255)
                                                                          acacCAGTACGCCGCCGATCAGCCAGACCACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTg                                                                          <  7:182530/149‑1 (MQ=255)
                                                                           cacCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTgg                                                                         <  4:421550/149‑1 (MQ=255)
                                                                            acCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTggg                                                                        <  6:321786/149‑1 (MQ=255)
                                                                              cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGATGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCg                                                                                                 >  1:390025/1‑122 (MQ=255)
                                                                              cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGATGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCg                                                                                                 <  2:390025/122‑1 (MQ=255)
                                                                              cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGcc                                                                      <  1:171220/149‑1 (MQ=255)
                                                                              cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTAGCGGATAGACTCCAGCTCGCGCAACTGGGAGATGTACGAGCCGGTGGACTTGGGcc                                                                      >  2:138118/1‑149 (MQ=255)
                                                                              cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATAAGGTAGCGGATCGACACCAGCTCGCGCAACGGCGAGAATAACGAGCCGGTGGACTTGGGcc                                                                      >  2:390870/1‑149 (MQ=255)
                                                                              cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTAGCGGTTAAGGAAGTGAAAAGGTAGCGGATCGACAACAGCAAGCGCAACAGCGAGATGAAAGAGCCGGTGGAAGTGGGcg                                                                      >  7:272790/1‑148 (MQ=255)
                                                                                gTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTcgc                                                                                                          >  7:96024/1‑111 (MQ=255)
                                                                                gTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCgag                                                                                               >  6:237527/1‑122 (MQ=255)
                                                                                gTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCgag                                                                                               <  5:237527/122‑1 (MQ=255)
                                                                                gTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGc                                                                    <  2:15469/149‑1 (MQ=255)
                                                                                 tACGCCGCGGATCAGCCAGAACACGAACAGGAACTGCAGGAAGAACGGCAGGTCGGCCAGCACCTTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCa                                                                   <  8:334810/149‑1 (MQ=255)
                                                                                 tACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGGGGACTTGGGCCGCa                                                                   >  8:398247/1‑149 (MQ=255)
                                                                                       gccgATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGt                                                             >  1:149285/1‑149 (MQ=255)
                                                                                          gATCAGCCAGACCACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgc                                                          <  4:2400/149‑1 (MQ=255)
                                                                                              aGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgca                                                       <  1:390870/148‑1 (MQ=255)
                                                                                               gCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCCTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTACAGCTCGCGCAACTGCGAGATGAAAGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcagg                                                     >  4:56019/1‑149 (MQ=255)
                                                                                               gCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCACCACCCTCGCGGTGCTGGACGTGATAAGGTCGCGGATAGACTCAACCACGCGCAAAAGCGAGATGAACCAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcagg                                                     >  5:292721/1‑149 (MQ=255)
                                                                                                 cAGAACACGAACAGGAACAGCTGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGAc                                                                             >  4:351016/1‑123 (MQ=255)
                                                                                                   gAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgca                                                 <  3:191382/149‑1 (MQ=255)
                                                                                                    aaCACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCCCCACCCTAGCGGTGCGGGCCGTGATCAGGTCGCGGATAGACTCCAGCTCGCGCAACAGCCACATGAACCAGCCCGGGGACATGGGCCGCCCCGAGTTgggacggcgcag                                                >  4:233905/1‑149 (MQ=255)
                                                                                                    aaCACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgcag                                                >  7:7299/1‑149 (MQ=255)
                                                                                                         gaacaggaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCgg                                                                                  >  3:114787/1‑110 (MQ=255)
                                                                                                         gaacaggaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCgg                                                                                  <  4:114787/110‑1 (MQ=255)
                                                                                                           acaggaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCg                                                                     >  5:396486/1‑121 (MQ=255)
                                                                                                           acaggaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCg                                                                     <  6:396486/121‑1 (MQ=255)
                                                                                                           acaggaacagCAGGAAGAACGGCAGGTCGGCCACCACCGTAGCGGTGCTGGACGTGATAAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATgg                                         >  8:69903/1‑149 (MQ=255)
                                                                                                              ggaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTTATCAGGTGGCGGAGCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCg                                      <  1:317203/149‑1 (MQ=255)
                                                                                                               gaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCg                                      >  5:354937/1‑148 (MQ=255)
                                                                                                               gaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGCGGACTTGGGCCGCACCGAGTTgcgcaggc                                                    <  3:235093/134‑1 (MQ=255)
                                                                                                                 acagCAGGAAGAACGGCAGGTCGGCCAGCACCGTAGCGGTGCTTGACGTGATAAGGTCGCGGATAGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACAGAGTTGCGGAGTCGCAGGGCCTGGCCg                                      >  8:172105/1‑146 (MQ=255)
                                                                                                                  cagcagGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGa                                  >  2:331874/1‑149 (MQ=255)
                                                                                                                  cagcagGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGa                                  <  6:32032/149‑1 (MQ=255)
                                                                                                                     cagGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGAc                                 >  1:237120/1‑147 (MQ=255)
                                                                                                                     cagGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGAc                                 >  4:36341/1‑147 (MQ=255)
                                                                                                                     cagGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCa                               <  3:233905/149‑1 (MQ=255)
                                                                                                                       ggAAGAACGGAAGGTGGGCCAGCACCGGCGCGGTGCTGGACGTGAGCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAgg                             <  7:61838/149‑1 (MQ=255)
                                                                                                                        gaagaaCGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGt                            <  4:282502/149‑1 (MQ=255)
                                                                                                                               ggCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCAc                     <  8:186392/149‑1 (MQ=255)
                                                                                                                               ggCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCAc                     >  5:177531/1‑149 (MQ=255)
                                                                                                                                gCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCAcc                    <  2:237120/149‑1 (MQ=255)
                                                                                                                                 cAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTTCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCAccc                   <  1:262632/149‑1 (MQ=255)
                                                                                                                                   ggTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGc                 <  6:220591/149‑1 (MQ=255)
                                                                                                                                    gTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCg                  >  1:131994/1‑147 (MQ=255)
                                                                                                                                     tCGTCCAGCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAg               <  3:291718/149‑1 (MQ=255)
                                                                                                                                       gtccAGCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGACGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAg               >  4:291718/3‑147 (MQ=255)
                                                                                                                                        tccAGCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTc            >  5:376021/2‑149 (MQ=255)
                                                                                                                                        gCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGt                            <  3:28190/133‑1 (MQ=255)
                                                                                                                                        gCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGt                            >  4:28190/1‑133 (MQ=255)
                                                                                                                                         ccAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCg           >  6:329241/1‑149 (MQ=255)
                                                                                                                                         ccAGCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCAAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCg           >  4:356022/1‑149 (MQ=255)
                                                                                                                                              aCCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGgcgcg      >  1:232638/1‑149 (MQ=255)
                                                                                                                                                cGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACtt                                                                           <  6:321912/78‑1 (MQ=255)
                                                                                                                                                cGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACtt                                                                           >  5:321912/1‑78 (MQ=255)
                                                                                                                                                cGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGc                 >  7:300270/1‑136 (MQ=255)
                                                                                                                                                cGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGc                 <  8:300270/136‑1 (MQ=255)
                                                                                                                                                  tCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTg  >  2:229325/1‑149 (MQ=255)
                                                                                                                                                  tCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTg  >  6:366500/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GGCCAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTG  >  NC_002947/924173‑924467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑