Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 924,321 G→A 7.8% A291V (GCG→GTG) ‡ PP_0804 ← protein secretion ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947924,3210GA 7.8% 285.8 / 9.3 102A291V (GCG→GTG) ‡PP_0804protein secretion ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (46/48);  new base A (4/4);  total (50/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 105 total aligned reads displayed.

CAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCC  >  NC_002947/924176‑924469
                                                                                                                                                 |                                                                                                                                                    
cAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCgg                                                                                                                                                   >  7:149753/1‑149 (MQ=255)
  aCCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTg                                                                                                                                                 >  3:421550/1‑149 (MQ=255)
  aCCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTg                                                                                                                                                 <  4:184099/149‑1 (MQ=255)
     gTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTg                                                                                                                                              >  1:238160/1‑149 (MQ=255)
     gTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTg                                                                                                                                              >  4:191382/1‑149 (MQ=255)
      tGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGCGACGGTGCTgg                                                                                                                                             <  6:106900/149‑1 (MQ=255)
        ccAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGCGACGGTGCTggc                                                                                                                                            <  4:32529/148‑2 (MQ=255)
          aGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGt                                                                                                                                         >  3:52189/1‑149 (MQ=255)
            gcgcCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGa                                                                                                                                       <  5:140689/149‑1 (MQ=255)
              gcCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATc                                                                                                                                     <  1:161882/149‑1 (MQ=255)
                 tttGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAgg                                                                                                                                  >  5:220591/1‑149 (MQ=255)
                     tgCAATCCACGCGGGCCTCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTcgc                                                                                                                              <  8:337058/149‑1 (MQ=255)
                     tgCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATAAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCCGGTcgc                                                                                                                              >  8:311597/1‑149 (MQ=255)
                      gcaagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTcgc                                                                                                                              <  8:149753/148‑1 (MQ=255)
                       caagcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCgg                                                                                                                            <  1:190078/149‑1 (MQ=255)
                         agcaaCCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGAt                                                                                                                          <  2:368456/149‑1 (MQ=255)
                              cccGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGTCCAGCACCGCGACGGTGCt                                                                                                                                               <  6:312762/123‑1 (MQ=255)
                                 gggCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCa                                                                                                                  <  3:163333/149‑1 (MQ=255)
                                  ggCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAg                                                                                                                 <  2:245243/149‑1 (MQ=255)
                                         ccATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACg                                                                                                                                          <  7:233848/117‑1 (MQ=255)
                                         ccATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCgcgc                                                                                                          <  8:83542/149‑1 (MQ=255)
                                          cATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCgcgc                                                                                                          >  6:13725/1‑148 (MQ=255)
                                            tGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAAc                                                                                                       >  8:295219/1‑149 (MQ=255)
                                                cagcagCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCg                                                                                                   <  6:162986/149‑1 (MQ=255)
                                                 agcagcGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCga                                                                                                  <  7:22239/149‑1 (MQ=255)
                                                 agcagcGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCga                                                                                                  <  2:238160/149‑1 (MQ=255)
                                                  gcagcGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGc                                                                                                    >  3:334522/1‑146 (MQ=255)
                                                    agcGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGAt                                                                                               <  3:386075/149‑1 (MQ=255)
                                                     gcgcGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGc                                                                                                    <  2:419896/143‑1 (MQ=255)
                                                     gcgcGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGc                                                                                                    >  1:419896/1‑143 (MQ=255)
                                                      cgcgACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGa                                                                                             <  3:89942/149‑1 (MQ=255)
                                                            cAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGc                                                                                       <  4:334522/149‑1 (MQ=255)
                                                              gCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCACGTCGGCCAGCACCGTCGCGGTGCTGGACCTGATCAGGTCGCGGATCGACTCCAGATCGCGCAACTGCGAGATGAACGAGCCg                                                                                     >  3:304946/1‑149 (MQ=255)
                                                               cGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCgg                                                                                    <  7:279718/149‑1 (MQ=255)
                                                                  gATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTg                                                                                  >  5:321786/1‑148 (MQ=255)
                                                                     gAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACt                                                                              >  3:282502/1‑149 (MQ=255)
                                                                     gAACACCAGTACGCCGCCGATCAACCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACt                                                                              >  5:314421/1‑149 (MQ=255)
                                                                       acacCAGTACGCCGCCGATCAGCCAGACCACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTg                                                                            <  7:182530/149‑1 (MQ=255)
                                                                        cacCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTgg                                                                           <  4:421550/149‑1 (MQ=255)
                                                                        cacCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTgg                                                                           <  3:326887/149‑1 (MQ=255)
                                                                         acCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTggg                                                                          <  6:321786/149‑1 (MQ=255)
                                                                           cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGATGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCg                                                                                                   >  1:390025/1‑122 (MQ=255)
                                                                           cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGATGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCg                                                                                                   <  2:390025/122‑1 (MQ=255)
                                                                           cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGcc                                                                        <  1:171220/149‑1 (MQ=255)
                                                                           cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTAGCGGATAGACTCCAGCTCGCGCAACTGGGAGATGTACGAGCCGGTGGACTTGGGcc                                                                        >  2:138118/1‑149 (MQ=255)
                                                                           cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATAAGGTAGCGGATCGACACCAGCTCGCGCAACGGCGAGAATAACGAGCCGGTGGACTTGGGcc                                                                        >  2:390870/1‑149 (MQ=255)
                                                                           cAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTAGCGGTTAAGGAAGTGAAAAGGTAGCGGATCGACAACAGCAAGCGCAACAGCGAGATGAAAGAGCCGGTGGAAGTGGGcg                                                                        >  7:272790/1‑148 (MQ=255)
                                                                             gTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTcgc                                                                                                            >  7:96024/1‑111 (MQ=255)
                                                                             gTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCgag                                                                                                 >  6:237527/1‑122 (MQ=255)
                                                                             gTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCgag                                                                                                 <  5:237527/122‑1 (MQ=255)
                                                                             gTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGc                                                                      <  2:15469/149‑1 (MQ=255)
                                                                              tACGCCGCGGATCAGCCAGAACACGAACAGGAACTGCAGGAAGAACGGCAGGTCGGCCAGCACCTTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCa                                                                     <  8:334810/149‑1 (MQ=255)
                                                                              tACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGGGGACTTGGGCCGCa                                                                     >  8:398247/1‑149 (MQ=255)
                                                                                    gccgATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGt                                                               >  1:149285/1‑149 (MQ=255)
                                                                                       gATCAGCCAGACCACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgc                                                            <  4:2400/149‑1 (MQ=255)
                                                                                           aGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgca                                                         <  1:390870/148‑1 (MQ=255)
                                                                                            gCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCCTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTACAGCTCGCGCAACTGCGAGATGAAAGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcagg                                                       >  4:56019/1‑149 (MQ=255)
                                                                                            gCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCACCACCCTCGCGGTGCTGGACGTGATAAGGTCGCGGATAGACTCAACCACGCGCAAAAGCGAGATGAACCAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcagg                                                       >  5:292721/1‑149 (MQ=255)
                                                                                              cAGAACACGAACAGGAACAGCTGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGAc                                                                               >  4:351016/1‑123 (MQ=255)
                                                                                                gAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgca                                                   <  3:191382/149‑1 (MQ=255)
                                                                                                 aaCACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCCCCACCCTAGCGGTGCGGGCCGTGATCAGGTCGCGGATAGACTCCAGCTCGCGCAACAGCCACATGAACCAGCCCGGGGACATGGGCCGCCCCGAGTTgggacggcgcag                                                  >  4:233905/1‑149 (MQ=255)
                                                                                                 aaCACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTgcgcaggcgcag                                                  >  7:7299/1‑149 (MQ=255)
                                                                                                      gaacaggaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCgg                                                                                    >  3:114787/1‑110 (MQ=255)
                                                                                                        acaggaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCg                                                                       >  5:396486/1‑121 (MQ=255)
                                                                                                        acaggaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCg                                                                       <  6:396486/121‑1 (MQ=255)
                                                                                                        acaggaacagCAGGAAGAACGGCAGGTCGGCCACCACCGTAGCGGTGCTGGACGTGATAAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATgg                                           >  8:69903/1‑149 (MQ=255)
                                                                                                           ggaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTTATCAGGTGGCGGAGCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCg                                        <  1:317203/149‑1 (MQ=255)
                                                                                                            gaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCg                                        >  5:354937/1‑148 (MQ=255)
                                                                                                            gaacagCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGCGGACTTGGGCCGCACCGAGTTgcgcaggc                                                      <  3:235093/134‑1 (MQ=255)
                                                                                                              acagCAGGAAGAACGGCAGGTCGGCCAGCACCGTAGCGGTGCTTGACGTGATAAGGTCGCGGATAGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACAGAGTTGCGGAGTCGCAGGGCCTGGCCg                                        >  8:172105/1‑146 (MQ=255)
                                                                                                               cagcagGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGa                                    >  2:331874/1‑149 (MQ=255)
                                                                                                               cagcagGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGa                                    <  6:32032/149‑1 (MQ=255)
                                                                                                                  cagGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGAc                                   >  4:36341/1‑147 (MQ=255)
                                                                                                                  cagGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGAc                                   >  1:237120/1‑147 (MQ=255)
                                                                                                                  cagGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCa                                 <  3:233905/149‑1 (MQ=255)
                                                                                                                    ggAAGAACGGAAGGTGGGCCAGCACCGGCGCGGTGCTGGACGTGAGCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAgg                               <  7:61838/149‑1 (MQ=255)
                                                                                                                     gaagaaCGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGt                              <  4:282502/149‑1 (MQ=255)
                                                                                                                            ggCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCAc                       >  5:177531/1‑149 (MQ=255)
                                                                                                                            ggCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCAc                       <  8:186392/149‑1 (MQ=255)
                                                                                                                             gCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCAcc                      <  2:237120/149‑1 (MQ=255)
                                                                                                                              cAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTTCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCAccc                     <  1:262632/149‑1 (MQ=255)
                                                                                                                                ggTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGc                   <  6:220591/149‑1 (MQ=255)
                                                                                                                                 gTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCg                    >  1:131994/1‑147 (MQ=255)
                                                                                                                                  tCGTCCAGCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAg                 <  3:291718/149‑1 (MQ=255)
                                                                                                                                    gtccAGCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGACGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAg                 >  4:291718/3‑147 (MQ=255)
                                                                                                                                     tccAGCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTc              >  5:376021/2‑149 (MQ=255)
                                                                                                                                     gCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGt                              >  4:28190/1‑133 (MQ=255)
                                                                                                                                     gCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGt                              <  3:28190/133‑1 (MQ=255)
                                                                                                                                      ccAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCg             >  6:329241/1‑149 (MQ=255)
                                                                                                                                      ccAGCACCGCGACGGTGCTGGACGTGATCAGGTCGCGGATCGACTCAAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCg             >  4:356022/1‑149 (MQ=255)
                                                                                                                                           aCCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGgcgcg        >  1:232638/1‑149 (MQ=255)
                                                                                                                                             cGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACtt                                                                             <  6:321912/78‑1 (MQ=255)
                                                                                                                                             cGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACtt                                                                             >  5:321912/1‑78 (MQ=255)
                                                                                                                                             cGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGc                   >  7:300270/1‑136 (MQ=255)
                                                                                                                                             cGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGc                   <  8:300270/136‑1 (MQ=255)
                                                                                                                                               tCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTg    >  2:229325/1‑149 (MQ=255)
                                                                                                                                               tCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTg    >  6:366500/1‑149 (MQ=255)
                                                                                                                                                 gcgGTTCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGcc  <  4:304946/149‑1 (MQ=255)
                                                                                                                                                 gcgGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGcc  <  7:311597/149‑1 (MQ=255)
                                                                                                                                                 gcgGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGc   <  2:149285/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CAACCGTGCCAGGCGCCTTTGTGCAAGCAACCCGGGCAGCACCATGGCCAGCAGCGCGACCAGCGGGATGAACACCAGTACGCCGCCGATCAGCCAGAACACGAACAGGAACAGCAGGAAGAACGGCAGGTCGGCCAGCACCGTCGCGGTGCTGGACGTGATCAGGTCGCGGATCGACTCCAGCTCGCGCAACTGCGAGATGAACGAGCCGGTGGACTTGGGCCGCACCGAGTTGCGCAGGCGCAGGGCATGGCCGTAGACCAGGTCGCTCACCCGCAGGTCGGCGCGCTTGCC  >  NC_002947/924176‑924469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑