Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 942,955 G→A 5.5% N889N (AAC→AAT)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947942,9550GA 5.5% 271.8 / 3.1 91N889N (AAC→AAT) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (47/39);  new base A (2/3);  total (49/42)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

CCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCGGTGTGATATCCGG  >  NC_002947/942810‑943101
                                                                                                                                                   |                                                                                                                                                     
ccAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTg                                                                                                                                                    <  6:262673/149‑1 (MQ=37)
ccAGATTGATCGGGCTGTCGGGCGCGGTGGTCAGTGTTACATCGTACTCGGTAGCAAAAGAATCATTGCCGCTTGGGTCGGTCTGCAACAGGCTCAACTTCTCGCCAGGCTGTGCTGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGtt                                                                                                                                                     <  6:435553/148‑1 (MQ=11)
   gATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGTTCGATCAGGAAGGTGCCGTTGGCa                                                                                                                                                 <  5:111844/149‑1 (MQ=37)
        cTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑a                                                                                                                                          <  1:290939/148‑1 (MQ=37)
        cTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑Ac                                                                                                                                         >  7:129885/1‑149 (MQ=37)
        cTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCAACGGCAccttc                                                                                                                                            <  8:292745/149‑4 (MQ=255)
           gggCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGa                                                                                                                                       >  7:6265/1‑148 (MQ=37)
           gggCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCAACGGCAccttcc                                                                                                                                           <  3:374815/147‑5 (MQ=255)
            ggCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCT‑‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGacac                                                                                                                                    >  4:189536/1‑149 (MQ=37)
               tGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACAccc                                                                                                                                  >  6:134125/1‑149 (MQ=37)
                 tCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCAACGGCAccttc                                                                                                                                            <  6:56876/140‑4 (MQ=255)
                 tCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCAACGGCAccttc                                                                                                                                            >  5:56876/1‑137 (MQ=255)
                    ggCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGcc                                                                                                                             >  3:276395/1‑149 (MQ=37)
                       gcgGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGAt                                                                                                                          >  2:358888/1‑149 (MQ=37)
                       gcgGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGAt                                                                                                                          >  2:359567/1‑149 (MQ=37)
                             gtCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGt                                                                                                                    <  1:398913/149‑1 (MQ=39)
                                    gTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTg                                                                                                             <  8:129885/149‑1 (MQ=255)
                                    gTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCATGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATAAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTg                                                                                                             >  4:84528/1‑149 (MQ=255)
                                       cATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGc                                                                                                           <  3:201580/148‑1 (MQ=255)
                                            tACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACg                                                                                                     <  8:44545/149‑1 (MQ=255)
                                             aCTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGAGCCCGGTACCATTGGCATCACGc                                                                                                    >  8:193595/1‑149 (MQ=255)
                                              cTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGc                                                                                                    <  5:334696/148‑1 (MQ=255)
                                               tCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCAc                                                                                                  >  1:250076/1‑149 (MQ=255)
                                                 ggTAGCCACCGTAGGATTGCAGCTTGGGTCTGTCTGCACCAGGCTCAACTGCTCGCCTGGCTTTGCGGCTGCTTTGCAAGGTCGATGAGTAAGGTGCGGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCTCGc                                                                                                    <  1:135692/147‑1 (MQ=14)
                                                  gTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGc                                                                                                                                                  <  4:171572/101‑1 (MQ=40)
                                                  gTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGc                                                                                                                                                  >  3:171572/1‑101 (MQ=40)
                                                     gCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGa                                                                                             <  3:105489/148‑1 (MQ=255)
                                                     gCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGAc                                                                                            <  2:254452/149‑1 (MQ=255)
                                                      ccACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACa                                                                                           <  1:17698/149‑1 (MQ=255)
                                                      ccACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACa                                                                                           <  4:276395/149‑1 (MQ=255)
                                                      ccACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACa                                                                                           <  2:294287/149‑1 (MQ=255)
                                                      ccACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCACGCTCAACAGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGAGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGa                                                                                             >  5:444133/1‑147 (MQ=255)
                                                       cACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTCCAATTGGCATCACGCACTTCGACAg                                                                                          >  8:402700/1‑149 (MQ=255)
                                                       cACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAg                                                                                          >  3:142586/1‑149 (MQ=255)
                                                        aCCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGt                                                                                         >  3:174645/1‑149 (MQ=255)
                                                         ccGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTg                                                                                        >  2:10081/1‑149 (MQ=255)
                                                         ccGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCAGCTG‑‑GGGCAAGGTCGATCAGGAAGGGGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTg                                                                                        <  1:422017/149‑1 (MQ=255)
                                                          cGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑CCGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTgg                                                                                       >  6:436457/1‑149 (MQ=255)
                                                          cGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTgg                                                                                       >  7:33526/1‑149 (MQ=255)
                                                               cATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTc                                                                                               <  2:398417/136‑1 (MQ=255)
                                                               cATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTc                                                                                               >  1:398417/1‑136 (MQ=255)
                                                                    ccGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTc                                                                             >  2:418087/1‑149 (MQ=255)
                                                                      gCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTcgc                                                                           >  1:107695/1‑149 (MQ=255)
                                                                          gggTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGcc                                                                                                                             >  3:58104/1‑95 (MQ=33)
                                                                          gggTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGcc                                                                                                                             <  4:58104/95‑1 (MQ=33)
                                                                            gtcggtcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAATGTGCCGTTGGCACCA‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGAc                                                                     >  5:307916/1‑149 (MQ=255)
                                                                                gtcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCt                                                                 >  3:273563/1‑149 (MQ=255)
                                                                                gtcTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCt                                                                 >  4:46576/1‑149 (MQ=255)
                                                                                      aCCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGc                                                           >  2:392921/1‑149 (MQ=255)
                                                                                      aCCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCATTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGc                                                           >  4:250996/1‑149 (MQ=255)
                                                                                       ccAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGcc                                                          <  5:134125/149‑1 (MQ=255)
                                                                                       ccAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCACGGcc                                                          >  3:162503/1‑149 (MQ=255)
                                                                                            cTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTac                                                      >  1:225544/1‑148 (MQ=255)
                                                                                             tCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTacca                                                    >  4:315746/1‑149 (MQ=255)
                                                                                             tCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTacca                                                    >  7:369771/1‑149 (MQ=255)
                                                                                              cAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTaccac                                                   >  3:149069/1‑149 (MQ=255)
                                                                                               aaCTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTaccac                                                   <  4:273563/148‑1 (MQ=255)
                                                                                                    cgcGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCCCCTACGTCCCCCCACTTGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTg                                                <  1:359567/147‑1 (MQ=17)
                                                                                                    cTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGc                                             >  4:372487/1‑149 (MQ=255)
                                                                                                    cTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGc                                             <  4:233611/149‑1 (MQ=255)
                                                                                                     tCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGcc                                            >  3:24311/1‑149 (MQ=255)
                                                                                                            gCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAgg                                     <  1:418087/149‑1 (MQ=255)
                                                                                                             cTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGa                                    <  3:46576/149‑1 (MQ=255)
                                                                                                                tgCGGGTG‑‑GGGCAAGGTCGATCAGGAAGGTGGCGTTGGGACCC‑‑‑ACGACACCGGTGCCGATCACGGTACCATTGTCATCACGCACTTCGACAGTGGCACGCGGTTCGCCGCGAGCGCTCAGGGCCGTACCACCTGGGCCGACCAGGatat                                 <  6:444133/149‑1 (MQ=255)
                                                                                                                    gCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGt                             >  2:289061/1‑149 (MQ=255)
                                                                                                                    gCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGt                             >  4:334998/1‑149 (MQ=255)
                                                                                                                    gCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGt                             <  1:289061/149‑1 (MQ=255)
                                                                                                                    gCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGt                             <  3:334998/149‑1 (MQ=255)
                                                                                                                           ggCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCg                        <  3:372487/149‑1 (MQ=255)
                                                                                                                                ggTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCg                   <  8:6265/149‑1 (MQ=255)
                                                                                                                                ggTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCg                   >  7:387421/1‑149 (MQ=255)
                                                                                                                                ggTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCg                   >  8:363848/1‑149 (MQ=255)
                                                                                                                                 gTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCgg                  >  8:145762/1‑149 (MQ=255)
                                                                                                                                  tCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTg                                                                                        <  8:46398/78‑1 (MQ=255)
                                                                                                                                  tCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTg                                                                                        >  7:46398/1‑78 (MQ=255)
                                                                                                                                     aTCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCgg              >  6:69852/1‑149 (MQ=255)
                                                                                                                                      tCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCGgt             <  8:369771/149‑1 (MQ=255)
                                                                                                                                        aGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCGgtgt           <  3:84528/149‑1 (MQ=255)
                                                                                                                                         ggAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCGGtgtg          <  8:33526/149‑1 (MQ=255)
                                                                                                                                         ggAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCGGtgtg          <  7:145762/149‑1 (MQ=255)
                                                                                                                                          gAAGGTGCCGTTGGCACCC‑‑‑ACGGCACCCGTGCCGATCACGGTACCATTGGCATCACGCAGTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCGGTGTGa         >  5:219202/1‑149 (MQ=255)
                                                                                                                                          gAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCGGTGTGa         >  2:353405/1‑149 (MQ=255)
                                                                                                                                          gAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCGGTGTGa         >  6:312667/1‑149 (MQ=255)
                                                                                                                                          gAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTCGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCGGTGTGa         >  4:27674/1‑149 (MQ=255)
                                                                                                                                          gAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATAACGGTAACATTGGGATCACGCACTTCGACATTGGCACCCGGTTGGCCGCCACCGCTCAGGGGCCTACCACCTGGGCCGGCCAGGAAATCGGTGAACGCATCg                   >  1:60300/1‑139 (MQ=18)
                                                                                                                                          gAAGGTGACGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCg                   <  2:60300/139‑1 (MQ=255)
                                                                                                                                                gCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAg                                                              <  5:211763/90‑1 (MQ=255)
                                                                                                                                                gCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAg                                                              >  6:211763/1‑90 (MQ=255)
                                                                                                                                                gCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCGGTGTGATATCCg   >  4:392361/1‑149 (MQ=255)
                                                                                                                                                 ccGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCGGTGTGATATCCgg  <  1:10081/149‑1 (MQ=255)
                                                                                                                                                 ccGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGCTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCGGTGTGATATCCg   <  6:307916/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCAGATTGCTCGGGCTGTCGGGCGCGGTGGTCAGTGGTACATCGTACTCGGTAGCCACCGAAGCATTGCCGCTTGGGTCGGTCTGCACCAGGCTCAACTGCTCGCCCGGCTGTGCGGCTG‑‑GGGCAAGGTCGATCAGGAAGGTGCCGTTGGCACCC‑‑‑ACGACACCCGTGCCGATCACGGTACCATTGGCATCACGCACTTCGACAGTGGCACCCGGTTCGCCGCGACCGCTCAGGGCCGTACCACCTGGGCCGACCAGGATATCGGTGACCGCATCGGGCGGTGTGATATCCGG  >  NC_002947/942810‑943101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑