Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 969,604 G→A 5.2% A173T (GCC→ACC)  queA → S‑adenosylmethionine:tRNA ribosyltransferase‑isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947969,6040GA 5.2% 302.7 / 3.2 96A173T (GCC→ACC) queAS‑adenosylmethionine:tRNA ribosyltransferase‑isomerase
Reads supporting (aligned to +/- strand):  ref base G (49/42);  new base A (2/3);  total (51/45)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

GCTGTTCGAACTTCGCTTCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAGATCGAAGACCACCACATGCA  >  NC_002947/969456‑969752
                                                                                                                                                    |                                                                                                                                                     
gCTGTTCGAACTTCGCTTCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTg                                                                                                                                                       <  5:183180/149‑1 (MQ=255)
 cTGTTCGAACTTCGCTTCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGc                                                                                                                                                      <  7:312620/149‑1 (MQ=255)
    ttCGAACTTCGCTTCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCcgc                                                                                                                                                   <  6:135329/149‑1 (MQ=255)
      cGAACTTCGCTTCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCcgcg                                                                                                                                                  <  7:8904/148‑1 (MQ=255)
        aaCTTCGCTTCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGcc                                                                                                                                                >  5:438313/1‑148 (MQ=255)
        aaCTTCGCTTCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGcc                                                                                                                                                >  3:273617/1‑148 (MQ=255)
          cTTCGCTTCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCTCATGCCGCTGCCGCCCTATATCGACCGACGGGTCGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGCAACCGCGccgg                                                                                                                                              <  2:142875/148‑3 (MQ=255)
           ttCGCTTCACCGAAGAGGTGCTGCCGTTGCTCGACCGAGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCACGCCTAcc                                                                                                                                            >  1:297192/1‑149 (MQ=255)
             cGCTTCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCg                                                                                                                                           >  1:381036/1‑148 (MQ=255)
              gCTTCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCa                                                                                                                                         <  4:429264/149‑1 (MQ=255)
               ctccACCGAAGTGGGGCTGCGGTTGCGCGACCGTGTCGGCCACTTGCCGCTGCCGCCCTATATCCACCGCCCGGACTTGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAg                                                                                                                                        <  2:208364/146‑1 (MQ=255)
               cTTCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTCCCGCAg                                                                                                                                        >  4:374397/1‑149 (MQ=255)
                ttCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAgg                                                                                                                                       >  1:253358/1‑149 (MQ=255)
                ttCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAgg                                                                                                                                       <  4:37334/149‑1 (MQ=255)
                  cACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGcc                                                                                                                                     >  4:228630/1‑149 (MQ=255)
                  cACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGcc                                                                                                                                     >  8:34249/1‑149 (MQ=255)
                  cACCGAAGAGGGGGTGCCTTTGCTCCACCGTTGCGGCCACATGCCGCTGCCGCCATATATCCCCCGCCCGCACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAgg                                                                                                                                       <  6:238691/147‑1 (MQ=255)
                    ccGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTg                                                                                                                                   <  6:35769/149‑1 (MQ=255)
                    ccGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTg                                                                                                                                   <  6:187883/149‑1 (MQ=255)
                     cGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGc                                                                                                                                  <  7:124014/149‑1 (MQ=255)
                            gTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTcga                                                                                                                           >  5:265286/1‑149 (MQ=255)
                             tgctgcCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTcgac                                                                                                                          >  6:121484/1‑149 (MQ=255)
                                tgcCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAg                                                                                                                       <  6:265286/149‑1 (MQ=255)
                                 gcCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCa                                                                                                                                 >  8:153989/1‑138 (MQ=255)
                                 gcCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCa                                                                                                                                 <  7:153989/138‑1 (MQ=255)
                                    gTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGgcgc                                                                                                                   >  6:70435/1‑149 (MQ=255)
                                    gTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGgcgc                                                                                                                   >  2:124884/1‑149 (MQ=255)
                                        cTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGTTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCgctgc                                                                                                                <  6:438313/148‑1 (MQ=255)
                                         tCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGctgctg                                                                                                              >  7:4723/1‑149 (MQ=255)
                                           gACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGctgctg                                                                                                              <  7:34249/147‑1 (MQ=255)
                                            aCCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGa                                                                                                            >  7:157281/1‑148 (MQ=255)
                                                tgtCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGaa                                                                                                           >  5:436181/1‑145 (MQ=255)
                                                    ggCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGc                                                                                                   >  4:321176/1‑149 (MQ=255)
                                                          aTGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACaa                                                                                             <  2:253358/149‑1 (MQ=255)
                                                             ccgctgccgcCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAg‑g                                                                                          <  3:21162/148‑1 (MQ=255)
                                                             ccgctgccgcCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAg‑g                                                                                          <  8:279127/148‑1 (MQ=255)
                                                              cgctgccgcCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGc                                                                                        >  8:43118/1‑149 (MQ=255)
                                                               gctgccgcCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCg                                                                                       >  6:364881/1‑149 (MQ=255)
                                                               gctgccgcCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCg                                                                                       >  7:23926/1‑149 (MQ=255)
                                                                   ccgccCTATATCGCCCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGa                                                                                   <  3:228630/149‑1 (MQ=255)
                                                                      cccTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAgcg                                                                                <  8:23926/149‑1 (MQ=255)
                                                                       ccTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAgcgc                                                                               <  7:43118/149‑1 (MQ=255)
                                                                           tatCGACCGGCCTGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCTCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCTACAAG‑GGCGTCGAGCGCGcc                                                                            <  5:232392/148‑1 (MQ=255)
                                                                           tatCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCt                                                                           >  4:50978/1‑149 (MQ=255)
                                                                             tCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTc                                                                         <  2:101016/149‑1 (MQ=255)
                                                                              cGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCg                                                                        >  3:59172/1‑149 (MQ=255)
                                                                                aCCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCGGCGCGCCGGCGCGGTTACCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGt                                                                       >  4:313900/1‑148 (MQ=255)
                                                                                aCCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTc                                                                      >  3:335797/1‑149 (MQ=255)
                                                                                  cGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCAc                                                                    >  3:183659/1‑149 (MQ=255)
                                                                                    cccGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCAccc                                                                  >  5:261652/1‑149 (MQ=255)
                                                                                    cccGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCAccc                                                                  >  6:441400/1‑149 (MQ=255)
                                                                                     ccGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCGGCGCGCCGGCGCGGTTACCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCt                                                                 <  3:313900/149‑1 (MQ=255)
                                                                                     ccGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCt                                                                 <  4:273617/149‑1 (MQ=255)
                                                                                      cGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTg                                                                <  7:198350/149‑1 (MQ=255)
                                                                                       ggACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGc                                                               <  6:361849/149‑1 (MQ=255)
                                                                                           gAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCAtgt                                                           >  2:400812/1‑149 (MQ=255)
                                                                                             gggCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTgg                                                         >  5:338624/1‑149 (MQ=255)
                                                                                             gggCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTgg                                                         >  7:362006/1‑149 (MQ=255)
                                                                                             gggCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTgg                                                         <  4:272813/149‑1 (MQ=255)
                                                                                                cgcCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGgcg                                                      >  3:86538/1‑149 (MQ=255)
                                                                                                 gcCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGgcgc                                                     >  8:183315/1‑149 (MQ=255)
                                                                                                 gcCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGgcgc                                                     >  8:72822/1‑149 (MQ=255)
                                                                                                 gcCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGgcgc                                                     >  4:18788/1‑149 (MQ=255)
                                                                                                       cGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCAc                                               <  5:364881/149‑1 (MQ=255)
                                                                                                               cTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGc                                       >  5:360366/1‑149 (MQ=255)
                                                                                                                tACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGcc                                      >  8:172246/1‑149 (MQ=255)
                                                                                                                 aCCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCg                                     >  5:352096/1‑149 (MQ=255)
                                                                                                                  ccAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCgg                                    <  6:436181/149‑1 (MQ=255)
                                                                                                                  ccAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCg                                     <  8:4723/148‑1 (MQ=255)
                                                                                                                  ccAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCg                                     <  1:400812/148‑1 (MQ=255)
                                                                                                                   cAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGt                                   >  2:197273/1‑149 (MQ=255)
                                                                                                                    aGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTg                                  >  5:11827/1‑149 (MQ=255)
                                                                                                                    aGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTg                                  >  4:323013/1‑149 (MQ=255)
                                                                                                                          tgtACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAGTCGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCgg                               <  1:124884/147‑1 (MQ=255)
                                                                                                                          tgtACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCggg                              <  8:362006/147‑1 (MQ=255)
                                                                                                                          tgtACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGt                             <  5:121484/148‑1 (MQ=255)
                                                                                                                          tgtACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTc                            >  7:405627/1‑149 (MQ=255)
                                                                                                                          tgtACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTc                            <  8:157281/149‑1 (MQ=255)
                                                                                                                           gtACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGcccaagg                           >  5:326325/1‑143 (MQ=255)
                                                                                                                           gtACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGcccaagg                           >  5:332231/1‑143 (MQ=255)
                                                                                                                            tACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCg                           <  4:183659/148‑1 (MQ=255)
                                                                                                                                cccAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGAGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCGGCCGGTGCGGGTCGAc                         >  6:147478/1‑146 (MQ=255)
                                                                                                                                 ccAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGAc                         >  4:405253/1‑145 (MQ=255)
                                                                                                                                  cAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGAc                         >  8:279748/1‑144 (MQ=255)
                                                                                                                                  cAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAGAt                    <  1:197273/149‑1 (MQ=255)
                                                                                                                                     cgcgcCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAggactg                 >  1:374591/1‑144 (MQ=255)
                                                                                                                                      gcgcCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAGATCGaa                <  2:381036/149‑1 (MQ=255)
                                                                                                                                      gcgcCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAGATCGaa                <  7:172246/149‑1 (MQ=255)
                                                                                                                                      gcgcCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAGATCGaa                <  7:183315/149‑1 (MQ=255)
                                                                                                                                         taggCGCGGCAACCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCAtgt                                                           >  8:121928/3‑103 (MQ=255)
                                                                                                                                         taggCGCGGCAACCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCAtgt                                                           <  7:121928/101‑1 (MQ=255)
                                                                                                                                         ccGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAGATCGAAGac             <  2:260803/149‑1 (MQ=255)
                                                                                                                                          cGGCGCGGTTGCCGCGCCTACCACAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAGATCGAAGac             >  2:62779/1‑148 (MQ=255)
                                                                                                                                            gcgcGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAGATCGAAGaccac          <  3:50978/149‑1 (MQ=255)
                                                                                                                                                 gTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGCCAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAGATCGAAGACCACCACAt     >  8:171536/1‑149 (MQ=255)
                                                                                                                                                 gTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAGATCGAAGACCACCACAt     >  4:254687/1‑149 (MQ=255)
                                                                                                                                                 gTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAGATCGAAGACCACCACAt     <  4:335797/149‑1 (MQ=255)
                                                                                                                                                    gCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAGATCGAAGACCACCACATGCa  >  8:317491/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCTGTTCGAACTTCGCTTCACCGAAGAGGTGCTGCCGTTGCTCGACCGTGTCGGCCACATGCCGCTGCCGCCCTATATCGACCGCCCGGACGAGGGCGCCGACCGTGAGCGCTACCAGACTGTGTACGCCCAGCGCGCCGGCGCGGTTGCCGCGCCTACCGCAGGCCTGCACTTCGACGAGGCGCTGCTGGAAAAGATCGCCGACAAG‑GGCGTCGAGCGCGCCTTCGTCACCCTGCATGTGGGCGCCGGCACCTTCCAGCCGGTGCGGGTCGACAAGATCGAAGACCACCACATGCA  >  NC_002947/969456‑969752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑