Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,013,248 A→T 23.3% intergenic (+76/+55) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,013,2480AT23.3% 56.3 / 13.5 30intergenic (+76/+55)prfC/potF‑Ipeptide chain release factor 3/putrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (9/14);  new base T (3/4);  total (12/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.02e-01

CGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATC  >  NC_002947/1013101‑1013393
                                                                                                                                                   |                                                                                                                                                  
cGGGTCAAGCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCAACATTAAGGCCTTAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCTCAGGAATCACCAGACCTGGAGGTCAt                                                                                                                                                   <  5:240099/149‑1 (MQ=255)
cGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt                                                                                                                                                   <  5:168036/149‑1 (MQ=255)
  ggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                                                 <  8:312387/149‑1 (MQ=255)
  ggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                                                 >  6:106372/1‑149 (MQ=255)
   gTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgtg                                                                                                                                                <  5:365104/149‑1 (MQ=255)
   gTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgtg                                                                                                                                                <  4:15360/149‑1 (MQ=255)
      aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGAtt                                                                                                                                             >  5:86055/1‑148 (MQ=25)
       aCCTGGCGCTGATGGAAGATCGCTTGCCTGACATAAAGTTCCGCGCTACACGCGATCAACATTAAGGACTGAAATCGGGGCTTCTTTGCAGCCCTTCGCGTGCACGCCCGCTCCCACAGGAATCACCAGACCTTGAGGTCATTTGATAc                                                                                                                                            <  8:59648/149‑1 (MQ=37)
       aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGAttc                                                                                                                                            <  6:86055/149‑3 (MQ=25)
       aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc                                                                                                                                            <  5:106372/149‑1 (MQ=255)
           ggCGCTGTTGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGGGAGCAGCATTAAGGCCTGAATTCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCAGAGGTATCACTTGACCTCCAGGTCTGGTGTTTCCtgt                                                                                                                                        <  5:11318/149‑1 (MQ=21)
            gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACATGACCTCCAGGTCTGGTGATTCCtgt                                                                                                                                        <  4:425276/148‑1 (MQ=25)
               cTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCCGCCTAGCGGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGa                                                                                                                                    <  3:338096/149‑1 (MQ=17)
               cTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGCGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGa                                                                                                                                    <  2:272470/149‑1 (MQ=255)
                  aTGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGc                                                                                                                                  <  6:308233/148‑1 (MQ=255)
                                        tCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGa                                                                                                                                               >  4:399849/1‑113 (MQ=255)
                                        tCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGa                                                                                                                                               <  3:399849/113‑1 (MQ=255)
                                                                                                                  ccGCTCCCACAGGAATCACCATACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgc                                                           <  7:14173/122‑1 (MQ=255)
                                                                                                                  ccGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCTTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgc                                                           >  8:14173/1‑122 (MQ=255)
                                                                                                                          acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTc                                             >  6:145818/1‑128 (MQ=255)
                                                                                                                          acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTc                                             <  5:145818/128‑1 (MQ=255)
                                                                                                                          acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                        >  6:78098/1‑149 (MQ=255)
                                                                                                                              gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGGTCTTCGCCGGCATCGCCTCCAgc                    >  6:372304/1‑149 (MQ=255)
                                                                                                                                 tCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                        <  3:2986/142‑1 (MQ=255)
                                                                                                                                 tCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                        >  4:2986/1‑142 (MQ=255)
                                                                                                                                     atgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa               >  7:189744/3‑147 (MQ=37)
                                                                                                                                          ctccaGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCtt         >  1:320115/5‑149 (MQ=38)
                                                                                                                                          cTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTg        >  4:306842/1‑149 (MQ=255)
                                                                                                                                          cTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTg        >  1:393671/1‑149 (MQ=255)
                                                                                                                                                 tCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATc  <  5:78098/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCC‑GCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATC  >  NC_002947/1013101‑1013393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑