Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,039,270 G→C 9.0% P42R (CCT→CGT)  PP_0897 ← class 1 fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,039,2700GC 9.0% 263.6 / 14.7 89P42R (CCT→CGT) PP_0897class 1 fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base G (38/43);  new base C (5/3);  total (43/46)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01

ACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGG  >  NC_002947/1039123‑1039418
                                                                                                                                                   |                                                                                                                                                    
aCGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCGGCAgg                                                                                                                                                     >  4:97586/1‑149 (MQ=255)
 cGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGcagcag                                                                                                                                                      <  1:157830/147‑1 (MQ=255)
   cTCATGGTGGCGCCATCCGAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCTGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGa                                                                                                                                                  <  6:242586/149‑1 (MQ=255)
      aTGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTc                                                                                                                                               >  6:251095/1‑149 (MQ=255)
       tggtggCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTcc                                                                                                                                              <  7:210399/149‑1 (MQ=255)
         gtggCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCCTGCTGCACGCGATTccat                                                                                                                                            <  7:124550/149‑3 (MQ=255)
          tggCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCCTGCTGCACGCGATTccatc                                                                                                                                           <  6:97508/149‑4 (MQ=255)
            gcgcCATCCCAGCGCACGTCCACGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACg                                                                                                                                         >  8:176582/1‑149 (MQ=255)
             cgcCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGc                                                                                                                                        <  2:339832/149‑1 (MQ=255)
                  tCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGa                                                                                                                                     >  2:144404/1‑147 (MQ=255)
                  tCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATa                                                                                                                                   <  8:148610/149‑1 (MQ=255)
                    ccAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAgg                                                                                                                                 >  2:309861/1‑149 (MQ=255)
                    ccAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAg                                                                                                                                  <  5:213787/148‑1 (MQ=255)
                    ccAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAg                                                                                                                                  <  2:255989/148‑1 (MQ=255)
                    ccAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAg                                                                                                                                  <  7:203827/148‑1 (MQ=255)
                        cgcACGTCCATGCCCCCGCGGATGAACACGGTGACGATACCGGTCTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTc                                                                                                                             >  6:324190/1‑149 (MQ=255)
                         gcACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCg                                                                                                                            >  1:340479/1‑149 (MQ=255)
                          cACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGt                                                                                                                           <  4:47723/149‑1 (MQ=255)
                          cACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGt                                                                                                                           <  5:150455/149‑1 (MQ=255)
                            cGTCCACGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGc                                                                                                                         <  7:176582/149‑1 (MQ=255)
                             gTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCa                                                                                                                        <  1:221894/149‑1 (MQ=255)
                              tCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGAAt                                                                                                                       >  8:104497/1‑149 (MQ=255)
                               ccATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATg                                                                                                                      >  7:1608/1‑149 (MQ=255)
                               ccATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATg                                                                                                                      >  7:376547/1‑149 (MQ=255)
                               ccATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCAGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATg                                                                                                                      >  1:341596/1‑149 (MQ=255)
                                    cccACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGTCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCt                                                                                                                  <  5:183530/148‑1 (MQ=255)
                                      cACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGa                                                                                                               <  2:249465/149‑1 (MQ=255)
                                      cACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGa                                                                                                               >  3:257924/1‑149 (MQ=255)
                                      cACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGa                                                                                                               <  2:340479/149‑1 (MQ=255)
                                       aCGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGAt                                                                                                              <  5:336003/149‑1 (MQ=255)
                                          cgGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGaa                                                                                                           <  1:318506/149‑1 (MQ=255)
                                           ggATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGaaa                                                                                                          <  7:15377/149‑1 (MQ=255)
                                             aTGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATc                                                                                                        <  1:76711/149‑1 (MQ=255)
                                                      gTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGggtg                                                                                               >  1:344802/1‑149 (MQ=255)
                                                         aCGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGgta                                                                                            <  8:1608/149‑1 (MQ=255)
                                                           gATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTg                                                                                                                 <  2:266179/126‑1 (MQ=255)
                                                           gATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTg                                                                                                                 >  1:266179/1‑126 (MQ=255)
                                                           gATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGgtag                                                                                           >  1:301160/1‑148 (MQ=255)
                                                           gATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGgtag                                                                                           >  7:148507/1‑148 (MQ=255)
                                                           gATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGgtag                                                                                           >  1:299613/1‑148 (MQ=255)
                                                           gATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGgtag                                                                                           >  5:84891/1‑148 (MQ=255)
                                                             tACAGGTGTCCTGGCAGATTGGACGAGGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTAc                                                                                        <  8:256842/149‑1 (MQ=255)
                                                               ccGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACga                                                                                      <  8:101324/149‑1 (MQ=255)
                                                               ccGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACga                                                                                      <  8:160271/149‑1 (MQ=255)
                                                               ccGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACga                                                                                      <  8:376547/149‑1 (MQ=255)
                                                                cGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTAc                                                                                        >  1:386649/1‑146 (MQ=255)
                                                                cGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTAc                                                                                        >  3:63338/1‑146 (MQ=255)
                                                                 ggTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACgaga                                                                                    <  2:301141/149‑1 (MQ=255)
                                                                  gtgtCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGAt                                                                                   >  3:100748/1‑149 (MQ=255)
                                                                        tgtcAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCCTGCAGCAGGCGATTCCTCACGCAGATAGGCCTAGTGCATGGCCTGCATGAAATCGAAGGGGTGGTAGTACGAGATGAAtt                                                                              >  1:227064/4‑148 (MQ=255)
                                                                        tGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTg                                                                             >  3:200005/1‑149 (MQ=255)
                                                                        tGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTg                                                                             >  7:410191/1‑149 (MQ=255)
                                                                        tGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTg                                                                             >  1:227060/1‑149 (MQ=255)
                                                                          gCAGATTGGACGATTGCCGGTGGCGCACATGCGCGAGTTGATCTGGTTCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCa                                                                           <  4:257924/149‑1 (MQ=255)
                                                                           cAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGGTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAg                                                                          <  3:240893/149‑1 (MQ=255)
                                                                            aGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAga                                                                         <  1:144404/149‑2 (MQ=255)
                                                                                       tGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGAc                                                              >  8:372778/1‑149 (MQ=255)
                                                                                       tGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGAc                                                              >  1:275844/1‑149 (MQ=255)
                                                                                         gCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGc                                                            >  1:266618/1‑149 (MQ=255)
                                                                                         gCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATAGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGc                                                            >  3:340583/1‑149 (MQ=255)
                                                                                           cGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGCGAGGAATTGCAGGGCGTCGGCGACGctc                                                          >  8:72625/1‑149 (MQ=255)
                                                                                           cGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGctc                                                          >  8:78459/1‑149 (MQ=255)
                                                                                            ggTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGctct                                                         <  2:386649/149‑1 (MQ=255)
                                                                                              actcgcgCATGTGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTg                                                        >  4:118794/4‑148 (MQ=255)
                                                                                               ggCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGaa                                                      <  2:344802/149‑1 (MQ=255)
                                                                                                   cacaTGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGTGGTGGTAGTTGGATATTAATTGCAGGGCGTCGGCGACGCTCTGAATCAg                                                  <  2:243280/149‑1 (MQ=255)
                                                                                                    acaTGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAgg                                                 >  2:268178/1‑149 (MQ=255)
                                                                                                     caTGCGCGAGTTGATCAGGATCTGGGCGATGGACTCGCGGGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGAGGAAATCGACGGGGGGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGgt                                                <  7:72625/149‑1 (MQ=255)
                                                                                                     caTGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGgt                                                <  7:78459/149‑1 (MQ=255)
                                                                                                         cgcgAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTTCGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGgtcgtc                                            <  1:309861/149‑1 (MQ=255)
                                                                                                         cgcgAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGgtcgtc                                            <  4:100748/149‑1 (MQ=255)
                                                                                                             aGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTcttgc                                        <  1:268178/149‑1 (MQ=255)
                                                                                                               ttGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTcttgctt                                      <  5:251095/149‑1 (MQ=255)
                                                                                                                tGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTcttgctt                                      >  7:133946/1‑148 (MQ=255)
                                                                                                                 gATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTcttgcttg                                     <  8:148507/148‑1 (MQ=255)
                                                                                                                    cAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCa                                 >  2:240403/1‑149 (MQ=255)
                                                                                                                      ggATCTGGTCGATTGAATCGCGTTCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACg                               <  6:84891/149‑1 (MQ=255)
                                                                                                                      ggATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCATATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGGAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACg                               <  4:63338/149‑1 (MQ=255)
                                                                                                                       gATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACgg                              >  4:279781/1‑149 (MQ=255)
                                                                                                                             ggCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATg                         <  7:372778/148‑1 (MQ=255)
                                                                                                                             ggCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGAAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTATTACGAGAAGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATg                         <  5:324190/148‑1 (MQ=255)
                                                                                                                              gCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATAGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCa                       <  4:340583/149‑1 (MQ=255)
                                                                                                                                tgaggAATCGCCTGCTGCACGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAgc                     >  7:339598/4‑149 (MQ=255)
                                                                                                                                 gaggAATCGCCTGCTGCACGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAgcg                    >  8:11874/3‑149 (MQ=255)
                                                                                                                                 gaggAATCGCCTGCTGCACGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAgcg                    >  7:218373/3‑149 (MQ=255)
                                                                                                                                 gaggAATCGCCTGCTGCACGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAgcg                    >  5:69579/3‑149 (MQ=255)
                                                                                                                                  tGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAgcgc                   >  3:332690/1‑149 (MQ=255)
                                                                                                                                   ggAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAgcgcg                  >  3:248105/1‑149 (MQ=255)
                                                                                                                                   ggAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGCCGTCGGCGACGATCTGAATCAGGTCTACTTGCTTGAACACGGACATGCAgcgcg                  >  2:246382/1‑149 (MQ=255)
                                                                                                                                    gAATCGCCTGCTGCACGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCg                                                                   >  2:88226/1‑99 (MQ=255)
                                                                                                                                    gAATCGCCTGCTGCACGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCg                                                                   <  1:88226/99‑1 (MQ=255)
                                                                                                                                              cagcagGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTaaa       <  1:240403/149‑1 (MQ=255)
                                                                                                                                                 cagGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGAc    >  3:157507/1‑149 (MQ=255)
                                                                                                                                                  agGCGATTCCTCAAGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGTGTGGTAGTACGAGATCAATTGCAGGGCGTCGGCGACCCTCTGAATCAGGTCGTCATGCTAGATCACGGACATGCAGCGCGCGACTCTTAAAGACg   >  5:263809/1‑149 (MQ=255)
                                                                                                                                                   ggCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACgg  <  8:410191/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACGCTCATGGTGGCGCCATCCCAGCGCACGTCCATGCCCACGCGGATGAACACGGTGACGATACCGGTGTCCTGGCAGATTGGACGATGGCCGGTGGCGCACATGCGCGAGTTGATCAGGATCTGGGCGATGGAATCGCGTGCAGCAGGCGATTCCTCACGCAGATAGGCCTCGTGCATGGCCTGGATGAAATCGACGGGGTGGTAGTACGAGATGAATTGCAGGGCGTCGGCGACGCTCTGAATCAGGTCGTCTTGCTTGATCACGGTCATGCAGCGCGCTCCTCTTAAAGACGG  >  NC_002947/1039123‑1039418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑