Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,152,999 A→C 6.1% D575A (GAC→GCC)  edd → phosphogluconate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,152,9990AC 6.1% 237.7 / 3.4 78D575A (GAC→GCC) eddphosphogluconate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (32/41);  new base C (3/2);  total (35/43)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGGAGGCTTACGACGGCGGCCCGCTGGCACGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCTGGTTGGTGATATTGGCGGCACCAATGCCC  >  NC_002947/1152853‑1153141
                                                                                                                                                  |                                                                                                                                              
cccGGAGGCTTACGACGGCGGCCCGCTGGCACGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGAcc                                                                                                                                              >  7:405852/1‑149 (MQ=255)
   ggAGGCTTACGACGGCGGCCCGCTGGCACGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTgg                                                                                                                                           <  5:82838/149‑1 (MQ=255)
    gAGGCTTACGACGGCGGCCCGCTGGCACGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTggg                                                                                                                                          <  6:147838/149‑1 (MQ=255)
           acgacgGCGGCCCGCTGGCACGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTggg                                                                                                                                   >  4:133777/1‑149 (MQ=255)
           acgacgGCGGCCCGCTGGCACGGGTGCGCGATGGTGATATCGGGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTgg                                                                                                                                    <  8:300482/148‑1 (MQ=255)
            cgacgGCGGCCCGCTGGCACGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTggg                                                                                                                                   <  3:329327/148‑1 (MQ=255)
              acgGCGGCCCGCTGGCACGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGgcgc                                                                                                                                <  3:126937/149‑1 (MQ=255)
                ggcggcCCGCTGGCACGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGa                                                                                                                              <  7:89125/149‑1 (MQ=255)
                ggcggcCCGCTGGCACGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGa                                                                                                                              <  8:33453/149‑1 (MQ=255)
                   ggcCGGCTGGCACGGGTGGGCGATGGTGTTATCGTGCGGGTCGACGGAGTCGAAGGCAGGTTGCGGATCTTGGTGTCGGCCGATGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCt                                                                                                                           <  4:386693/149‑1 (MQ=255)
                   ggcCCGCTGGCACGGGTGCGTGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCt                                                                                                                           >  6:117024/1‑149 (MQ=255)
                    gcCCGCTGGCACGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTg                                                                                                                          >  4:429020/1‑149 (MQ=255)
                      cagCTGGCACGGGTGCGCGATGGTGATATCGTGCGGGTCGACGTAGTAGAAGTAACGTTGCGGATCATGGTGTCGGACGAAGAACTGGCCATCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGTGTGTGGGCGCGAGCTGtt                                                                                                                        >  1:103559/3‑149 (MQ=255)
                       cGCTGGCACGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTc                                                                                                                       <  5:424137/149‑1 (MQ=255)
                            gCACGGGGGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCt                                                                                                                   <  5:303331/148‑1 (MQ=255)
                              aCGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCa                                                                                                                <  8:231719/149‑1 (MQ=255)
                              aCGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCCACACCCCAGGTCGTTGCCCTGGGGTGCCGgcgg                                                                                                                                <  6:24626/133‑2 (MQ=37)
                              aCGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCCACACCCCAGGTCGTTGCCCTGGGGTGCCGgcgg                                                                                                                                >  5:24626/1‑132 (MQ=37)
                                gggTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATg                                                                                                              >  5:324641/1‑149 (MQ=255)
                                gggTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAACCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATg                                                                                                              >  8:410227/1‑149 (MQ=255)
                                  gTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGa                                                                                                                              >  7:128318/1‑131 (MQ=255)
                                  gTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGa                                                                                                                              <  8:128318/131‑1 (MQ=255)
                                      gcgATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCTGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATg                                                                                                        <  1:438026/149‑1 (MQ=255)
                                         aTGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCg                                                                                                     <  1:180320/149‑1 (MQ=255)
                                                     tGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCa                                                                                         <  1:71669/149‑1 (MQ=255)
                                                       cGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCaga                                                                                       <  7:332662/149‑1 (MQ=255)
                                                        gggTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCagag                                                                                      <  3:338310/149‑1 (MQ=255)
                                                         ggTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAg                                                                                                <  7:243484/138‑1 (MQ=255)
                                                         ggTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAg                                                                                                >  8:243484/1‑138 (MQ=255)
                                                          ggcGACGGCGGCGAAGGCACGTTGCGGCTCATGGTGTCGGCCGAAGAACTGGCCAGCCTTGATCTTCCTCCTGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCTCATGGCGTGCAGCCCGGCAGAGCa                                                                                    <  2:103559/147‑1 (MQ=255)
                                                          gTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCagag                                                                                      >  3:186917/1‑147 (MQ=255)
                                                          gTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCagag                                                                                      >  3:180218/1‑147 (MQ=255)
                                                           tCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAg                                                                                   <  5:117024/149‑1 (MQ=255)
                                                           tCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAg                                                                                   <  8:405852/149‑1 (MQ=255)
                                                              aCGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGgc                                                                                >  2:269179/1‑149 (MQ=255)
                                                              aCGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGgc                                                                                >  2:312235/1‑149 (MQ=255)
                                                                ggAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGCCCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCAGGCTCATGGCGTTCCGCCCGGCAGAGCAGGgcgc                                                                              >  5:262703/1‑149 (MQ=255)
                                                                 gAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGcc                                                                             <  6:382183/149‑1 (MQ=255)
                                                                      gAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGc                                                                        >  4:246915/1‑149 (MQ=255)
                                                                      gAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGc                                                                        >  3:174973/1‑149 (MQ=255)
                                                                       aaGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGcc                                                                       >  6:101059/1‑149 (MQ=255)
                                                                          gCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCttt                                                                    <  1:312235/149‑1 (MQ=255)
                                                                            aCGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTAc                                                                  >  5:311562/1‑149 (MQ=255)
                                                                            aCGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTAc                                                                  >  5:435073/1‑149 (MQ=255)
                                                                                    gATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGccc                                                          >  4:62912/1‑149 (MQ=255)
                                                                                     aTCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCt                                                         >  4:192195/1‑149 (MQ=255)
                                                                                       cATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTgg                                                       >  1:200286/1‑149 (MQ=255)
                                                                                        aTGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTgg                                                       <  4:165617/148‑1 (MQ=255)
                                                                                            tgtCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCAc                                                  <  6:195709/149‑1 (MQ=255)
                                                                                             gtCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCAcc                                                 <  6:311562/149‑1 (MQ=255)
                                                                                               cGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTc                                               <  3:246915/149‑1 (MQ=255)
                                                                                                   cGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCttt                                                                    >  1:417886/1‑124 (MQ=255)
                                                                                                   cGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCttt                                                                    <  2:417886/124‑1 (MQ=255)
                                                                                                    gaagaaCTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAAt                                           >  7:162421/1‑148 (MQ=255)
                                                                                                          cTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCTGCTTCATGCTCTTGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCAc                                    <  2:361762/149‑1 (MQ=255)
                                                                                                          cTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCAc                                    <  2:200286/149‑1 (MQ=255)
                                                                                                           tGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCa                                                                                    >  1:236266/1‑100 (MQ=255)
                                                                                                           tGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCa                                                                                    <  2:236266/100‑1 (MQ=255)
                                                                                                             gCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCtt                                                                     >  4:86297/1‑113 (MQ=255)
                                                                                                             gCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCtt                                                                     <  3:86297/113‑1 (MQ=255)
                                                                                                             gCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGaa                                        >  5:40500/1‑142 (MQ=255)
                                                                                                             gCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGaa                                        <  6:40500/142‑1 (MQ=255)
                                                                                                                  ccGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCtggt                            >  4:401935/1‑149 (MQ=255)
                                                                                                                  ccGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCtggt                            >  3:44873/1‑149 (MQ=255)
                                                                                                                  ccGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCtggt                            <  4:186917/149‑1 (MQ=255)
                                                                                                                  ccGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCtggt                            <  4:180218/149‑1 (MQ=255)
                                                                                                                       aGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCtggttggt                        <  3:192195/148‑1 (MQ=255)
                                                                                                                       aGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCtggttggt                        <  5:101059/148‑1 (MQ=255)
                                                                                                                       aGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCtggttggt                        <  3:429020/148‑1 (MQ=255)
                                                                                                                       aGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCTGGTTGGTg                       <  1:387850/149‑1 (MQ=255)
                                                                                                                          tGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCTGGTTGGTGata                    >  3:239764/1‑149 (MQ=255)
                                                                                                                               cccacACCCCAGGTCGTTGCCCTGGGGTGCCGGCGGCAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCTGGTTGGTGATATTggc               <  4:293667/145‑1 (MQ=37)
                                                                                                                                  acACCCCAGGTCGTTGCCCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCTGGTTGGTGATATTggcgg             >  6:189635/2‑148 (MQ=255)
                                                                                                                                        cAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCTGGTTGGTGATATTg                 <  3:44743/138‑1 (MQ=255)
                                                                                                                                        cAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCTGGTTGGTGATATTg                 >  4:44743/1‑138 (MQ=255)
                                                                                                                                        cAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCTGGTTGGTGATATTGGCGGCACCAAt      >  7:61386/1‑149 (MQ=255)
                                                                                                                                         aGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCTGGTTGGTGATATTGGCGGCACCAATg     <  1:269179/149‑1 (MQ=255)
                                                                                                                                            gCAACGACCTGGGGTGTGGGCGCGAGCTGTTAGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCATCTCAAATGAAGAACCTGCTGGTTGGTGATATTGGCGGCACCAATGccc  >  6:178784/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CCCGGAGGCTTACGACGGCGGCCCGCTGGCACGGGTGCGCGATGGTGATATCGTGCGGGTCGACGGAGTCGAAGGCACGTTGCGGATCATGGTGTCGGCCGAAGAACTGGCCAGCCGTGAGCTGCCGCCGGCACCCCAGGGCAACGACCTGGGGTGTGGGCGCGAGCTGTTCGGCTTCATGCGCATGGCGTTCAGCCCGGCAGAGCAGGGCGCCAGTGCCTTTACCTCGGCCCTGGAGCACCTCAAATGAAGCACCTGCTGGTTGGTGATATTGGCGGCACCAATGCCC  >  NC_002947/1152853‑1153141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑