Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	158,054	A→C	7.6%	N22T (AAC→ACC)	PP_0148 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	158,054	0	A	C	7.6%	188.2 / 3.1	62	N22T (AAC→ACC)	PP_0148	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/35);  new base C (3/2);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGTCTTGTGCCGGTAGTGGGCGCTATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGCGAGCCGAGCTGCTATGTGGCGCCTGCCAGTTGCAGCCGC  >  NC_002947/157906‑158202                                                                                                                                                     |                                                                                                                                                      ccGGTCTTGTGCCGGTAGTGGGCGCTATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCaa                                                                                                                                                       >  8:236292/1‑149 (MQ=255) ccGGTCTTGTGCCGGTAGTGGGCGCTATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCaa                                                                                                                                                       <  4:308083/149‑1 (MQ=255) ccGGTCTTGTGCCGGTAGTGGGCGCTATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCaa                                                                                                                                                       <  7:236292/149‑1 (MQ=255)   ggTCTTGTGCCGGTAGTGGGCGCTATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAAcc                                                                                                                                                     <  7:82040/149‑1 (MQ=255)    gTCTTGTGCCGGTAGTGGGCGCTATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCt                                                                                                                                                    <  2:85008/149‑1 (MQ=255)      cTTGTGCCGGTAGTGGGCGCTATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCACCCTGa                                                                                                                                                  >  2:234223/1‑149 (MQ=255)      cTTGTGCCGGTAGTGGGCGCTATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCACCCTGa                                                                                                                                                  >  2:227371/1‑149 (MQ=255)        tgtgCCGGTAGTGGGCGCTATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGAc                                                                                                                                                 <  2:221890/148‑1 (MQ=255)         gtgCCGGTAGTGGGCGCTATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACg                                                                                                                                                >  1:226693/1‑148 (MQ=255)            ccGGTAGTGGGCGCTATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGAc                                                                                                                                             <  3:65554/148‑1 (MQ=255)                  gTGGGCGCTATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGcc                                                                                                                                       <  1:2325/148‑1 (MQ=255)                          tATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑t                                                                                                                             >  1:166732/1‑149 (MQ=255)                            tCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCACCCTGACGGACATTGCCCACTGTCA‑tt                                                                                                                            <  1:227371/148‑1 (MQ=255)                            tCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCACCCTGACGGACATTGCCCACTGTCA‑tt                                                                                                                            <  1:234223/148‑1 (MQ=255)                              cTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGtt                                                                                                                         >  6:159290/1‑149 (MQ=255)                                 aaaGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGa                                                                                                                      <  5:159290/149‑1 (MQ=255)                                  aaGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGAt                                                                                                                     <  4:119342/149‑1 (MQ=255)                                  aaGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACAAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGAt                                                                                                                     <  7:300/149‑1 (MQ=255)                                    gggCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCt                                                                                                                   >  2:233179/1‑149 (MQ=255)                                     gggaaGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGTTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTg                                                                                                                  <  4:220638/146‑1 (MQ=255)                                     ggCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTg                                                                                                                  <  2:166732/149‑1 (MQ=255)                                      gCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGc                                                                                                                 <  5:405338/149‑1 (MQ=255)                                      gCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGc                                                                                                                 <  5:230691/149‑1 (MQ=255)                                             ttgagccagcTTTCGCTTAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCgg                                                                                                           <  5:52633/145‑1 (MQ=255)                                             ttCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGTGGTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCggg                                                                                                          >  3:43703/1‑149 (MQ=255)                                                agccagcTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTc                                                                                                        <  6:208084/148‑1 (MQ=255)                                                agccagcTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCa                                                                                                       <  8:81336/149‑1 (MQ=255)                                                agccagcTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATC‑‑‑‑GGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCgg                                                                                                   <  3:283408/149‑1 (MQ=255)                                                 gccagcTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCACCCTGACGGCCATTGCCGACTGGCA‑TTGTGCGATCTGCAGGCGGGTCAg                                                                                                      >  4:8720/1‑149 (MQ=255)                                                      cTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTg                                                                                                 <  7:186744/149‑1 (MQ=255)                                                          cGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGAc                                                                                              <  6:285340/148‑1 (MQ=255)                                                             tGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAACGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACtggt                                                                                          >  1:305909/1‑149 (MQ=255)                                                               aaGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCCGCGGTGGACtggtg                                                                                         <  7:368230/148‑1 (MQ=255)                                                               aaGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACtggtg                                                                                         <  2:226693/148‑1 (MQ=255)                                                               aaGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACtggtg                                                                                         <  4:328100/148‑1 (MQ=255)                                                                 gCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCCGCGGTGGACTggtggt                                                                                       >  4:367163/1‑148 (MQ=255)                                                                       gTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTgg                                                                                <  8:37303/149‑1 (MQ=255)                                                                        tGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGc                                                                               <  5:379319/149‑1 (MQ=255)                                                                                 gtaggtGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGc                                                                      >  1:344369/1‑149 (MQ=255)                                                                                  taggtGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGc                                                                      <  3:270305/148‑1 (MQ=255)                                                                                    ggtGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGcccc                                                                   >  7:105896/1‑149 (MQ=255)                                                                                      tGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGATGGAGGGCAACCTGACGGACATTGACCACTGTCA‑TTTTTAGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCGCGt                                                                 >  7:279211/1‑149 (MQ=255)                                                                                            aGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGTGTTATCGGCTGGAGGGCAACGTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTgg                                                            <  4:43703/148‑1 (MQ=255)                                                                                                    aCCGAAGGGGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGt                                                    <  3:8720/148‑1 (MQ=255)                                                                                                      cGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTg                                                   <  5:359008/147‑1 (MQ=255)                                                                                                      cGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTg                                                   >  6:359008/1‑147 (MQ=255)                                                                                                              gCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACggg                                          <  1:233179/148‑1 (MQ=255)                                                                                                                tGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGc                                         >  2:187555/1‑147 (MQ=255)                                                                                                                 gCCATTGTGGAGCCCTGCGTTAGCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCATTTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGc                                         >  7:66169/1‑147 (MQ=255)                                                                                                                   cATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTgg                                                  >  1:19989/1‑135 (MQ=255)                                                                                                                   cATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTgg                                                  <  2:19989/135‑1 (MQ=255)                                                                                                                    aTTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGcgagcc                                    >  6:318349/1‑148 (MQ=255)                                                                                                                    aTTGTGGAGCCCTGCGGTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACAGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGcgagccg                                   <  8:351630/149‑1 (MQ=255)                                                                                                                        tgGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGCGAGCCGAgct                               >  3:51738/1‑149 (MQ=255)                                                                                                                           aGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGCGAGCCGAgctgc                             >  5:204082/1‑148 (MQ=255)                                                                                                                             cccTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGcgagccga                                  >  7:364236/1‑141 (MQ=255)                                                                                                                             cccTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGcgagccga                                  <  8:364236/141‑1 (MQ=255)                                                                                                                               cTGCGTTATCGGCTGGAGGGCAACCTGAC‑GACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGCGAGCCGAGCTGCTAtgtg                       >  6:242737/1‑149 (MQ=255)                                                                                                                                 gCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGAGGGTCAGCGGTGGACTGGTGGTCAACTGGCTTACACTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGCGAGCCGAGCTGCTATGTgg                      >  7:145989/1‑149 (MQ=255)                                                                                                                                  cGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGCGAGCCGAGCTGCTATGTGgc                     <  2:305909/149‑1 (MQ=255)                                                                                                                                          gCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGCGAGCCGAGCTGCTATGTGGCGCCTGCCa             <  2:344369/149‑1 (MQ=255)                                                                                                                                                gggCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGCGAGCCGAGCTGCTATGTGGCGCCTGCCAGTTg         >  5:263163/1‑147 (MQ=255)                                                                                                                                                 ggCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGCGAGCCGAGCTGCTATGTGGCGCCTGCCAGTTGCAg      >  7:210234/1‑149 (MQ=255)                                                                                                                                                 ggCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGCGAGCCGAGCTGCTATGTGGCGCCTGCCAGTTGCAg      <  5:318349/149‑1 (MQ=255)                                                                                                                                                   cAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGCGAGCCGAGCTGCTATGTGGCGCCTGCCAGTTGCAGcc    <  6:107452/149‑1 (MQ=255)                                                                                                                                                     aCCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGCGAGCCGAGCTGCTATGTGGCGCCTGCCAGTTGCAGCCGc  <  8:105896/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                      CCGGTCTTGTGCCGGTAGTGGGCGCTATCCTAAAAGGGCAAGCTTTCAGCCAGCTTTCGCTGAAGCAAAGGTGACGAGAGGTAGGTGATGCAGGATGTAACCGAAGGTGGCTGCCATTGTGGAGCCCTGCGTTATCGGCTGGAGGGCAACCTGACGGACATTGCCCACTGTCA‑TTGTTCGATCTGCAGGCGGGTCAGCGGTGGACTGGTGGTCACCTGGCTTACCCTGCCCCGTTCTGGGTTCCAGTGGTTAACGGGCGAGCCGAGCTGCTATGTGGCGCCTGCCAGTTGCAGCCGC  >  NC_002947/157906‑158202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑