Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,237,648 T→C 5.9% E154G (GAG→GGG)  PP_1078 ← ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,237,6480TC 5.9% 248.1 / 4.3 85E154G (GAG→GGG) PP_1078ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (40/40);  new base C (3/2);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.43e-01

ACGGCAAAGGCTTCTTCCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGCGATAC  >  NC_002947/1237501‑1237792
                                                                                                                                                   |                                                                                                                                                 
aCGGCAAAGGCTTCTACCTGGTCATGGGTGACCAGAATGGCGCAGGTACCGCGGCTTTTGAGAATGTCGCGATCCTCATGGCTCAGGCGCCGGCGCACCTCCACGACGAGGATGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTc                                                                                                                                                  >  2:15955/1‑149 (MQ=255)
aCGGCAAAGACTTCTTCCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTc                                                                                                                                                  <  1:421027/149‑1 (MQ=255)
   gCAAAGGCTTCTTCCTGGTCATGGGTGACCAGAATGGCGATGGTACCGCGGCTTTTGAGAATGTAGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCggg                                                                                                                                               >  6:233067/1‑149 (MQ=255)
    cAAAGGCTTCTTCCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGgc                                                                                                                                              >  7:25771/1‑149 (MQ=255)
        ggCTTCTTCCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGGGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCTGCGCAGCTCCACGTCGAGGTTGGAGAATGGATCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCa                                                                                                                                          <  1:15955/149‑1 (MQ=255)
        ggCTTCTTCCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGgcgc                                                                                                                                            >  8:356264/1‑147 (MQ=255)
        ggCTTCTTCCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCa                                                                                                                                          <  2:279758/149‑1 (MQ=255)
         gCTTCTTCCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAg                                                                                                                                         >  3:277888/1‑149 (MQ=255)
          cttcttCCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAgc                                                                                                                                        >  5:78570/1‑149 (MQ=255)
                  tGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGc                                                                                                                                >  3:217029/1‑149 (MQ=255)
                       aTGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAggg                                                                                                                           >  1:323196/1‑149 (MQ=255)
                        tGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCGCAGGCGCCGGCGCAGCTCCACGTCGAGGGTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGc                                                                                                                          <  2:383888/149‑1 (MQ=255)
                         gggTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGc                                                                                                                          >  7:222605/1‑148 (MQ=255)
                            tGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGaca                                                                                                                      >  3:135783/1‑149 (MQ=255)
                            tGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTGGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGaca                                                                                                                      <  5:89577/149‑1 (MQ=255)
                             gACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGacac                                                                                                                     >  4:202005/1‑149 (MQ=255)
                              aCCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCCGCAGTTGCGGCTCGGGCGCCAGCGCCCGCGCCAGGGCGACACg                                                                                                                    >  7:399771/1‑149 (MQ=255)
                              aCCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACg                                                                                                                    >  1:361237/1‑149 (MQ=255)
                                 aGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTtg                                                                                                                 <  2:323196/149‑1 (MQ=255)
                                  gAATGGCGCTGGTACCGCGTCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTtgc                                                                                                                <  7:154337/149‑1 (MQ=255)
                                  gAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTtgc                                                                                                                >  5:398534/1‑149 (MQ=255)
                                    aTGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTtgctg                                                                                                              <  1:115800/149‑1 (MQ=255)
                                    aTGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTtgctg                                                                                                              <  3:77023/149‑1 (MQ=255)
                                        cgcTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTgg                                                                                                          <  8:222605/149‑1 (MQ=255)
                                          cTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGgcc                                                                                                        >  6:311111/1‑149 (MQ=255)
                                             gTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGgccgc                                                                                                      <  6:371637/148‑1 (MQ=255)
                                                   cgGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAg                                                                                              >  2:331797/1‑149 (MQ=255)
                                                       ttttGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCa                                                                                          >  5:53317/1‑149 (MQ=255)
                                                       ttttGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCCCGGgcgctg                                                                                                                                          <  7:374240/102‑3 (MQ=255)
                                                       ttttGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCCCGGgcgctg                                                                                                                                          >  8:374240/1‑100 (MQ=255)
                                                        tttGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCCGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCAt                                                                                         >  8:120218/1‑149 (MQ=255)
                                                           gagaATGTCGCTTACCTGTTGGCTCAGGCGCCGGCGCAGGTCGACGTCGTGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCTGGTCTGTCGGCAGCTCCCGGTCCAGGGCGAGACGTTGCTGCTGGACGG‑CGGTCAGCTCATGc                                                                                       <  8:399771/148‑1 (MQ=255)
                                                             gaATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGTCCGG‑CGGCCAGca                                                                                            <  7:325360/141‑2 (MQ=255)
                                                             gaATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCgg                                                                                     <  4:16356/148‑1 (MQ=255)
                                                             gaATGTCGCGAACCTCATGGCTCAGGCGCAGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGTCCGG‑CGGCCAGca                                                                                            >  8:325360/1‑140 (MQ=255)
                                                               aTGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGt                                                                                   >  5:343895/1‑148 (MQ=255)
                                                                tGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTaa                                                                                 <  1:383832/149‑1 (MQ=255)
                                                                  tCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAAcg                                                                               >  5:416599/1‑149 (MQ=255)
                                                                  tCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAAc                                                                                <  4:217029/148‑1 (MQ=255)
                                                                   cgcgAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAgg                                                                                                                            <  3:190416/104‑1 (MQ=255)
                                                                   cgcgAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAgg                                                                                                                            >  4:190416/1‑104 (MQ=255)
                                                                   cgcgAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCCGGCCAGGGCCACACGATGCTCCTGGCCGC‑CGGACAGCTCATCCGCGTAAcgc                                                                              >  8:411758/1‑149 (MQ=255)
                                                                    gcgAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAAcgcc                                                                             >  2:99460/1‑149 (MQ=255)
                                                                    gcgAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAAcgcc                                                                             <  6:53317/149‑1 (MQ=255)
                                                                     cgAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGgccgc                                                                                                      >  6:117285/1‑124 (MQ=255)
                                                                     cgAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGgccgc                                                                                                      <  5:117285/124‑1 (MQ=255)
                                                                     cgAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAAcgccc                                                                            >  5:58567/1‑149 (MQ=255)
                                                                      gAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAAcgcccg                                                                           <  6:443086/149‑1 (MQ=255)
                                                                       aaCCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAAcgcccgc                                                                          <  3:255292/149‑1 (MQ=255)
                                                                                    atgcgcCTGCTCAGCTCAACGTCGAGGTTTGAGAATGGCTCTTCAAGCAGCAGCAGTTGCGGCTCGGGCGACAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCGGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGa                                                              <  6:343895/146‑1 (MQ=255)
                                                                                    aGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAg                                                             >  2:380488/1‑149 (MQ=255)
                                                                                    aGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAg                                                             <  5:233067/149‑1 (MQ=255)
                                                                                     ggcgccggcgccGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGt                                                            <  5:311111/149‑1 (MQ=255)
                                                                                     ggCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGt                                                            <  6:416599/149‑1 (MQ=255)
                                                                                      gcgcCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGtt                                                           >  5:397116/1‑149 (MQ=255)
                                                                                      gcgcCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACCTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCAAGCCCGCCGAGtt                                                           >  8:98056/1‑149 (MQ=255)
                                                                                        gcCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGGTTg                                                         <  6:417022/149‑1 (MQ=255)
                                                                                         ccGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGa                                                        <  6:398927/149‑1 (MQ=255)
                                                                                              gcAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGc                                                   <  8:78166/149‑1 (MQ=255)
                                                                                                   tCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAg                                              >  6:400324/1‑149 (MQ=255)
                                                                                                     cACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGGCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGCTCGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCATCTCCa                                               <  1:331797/147‑1 (MQ=255)
                                                                                                     cACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTGTGACCAGCTCCAGCa                                            >  4:359859/1‑149 (MQ=255)
                                                                                                        gTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCt                                                  <  6:16470/140‑1 (MQ=255)
                                                                                                        gTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCt                                                  >  5:16470/1‑140 (MQ=255)
                                                                                                               ttGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGAt                                  >  3:208530/1‑149 (MQ=255)
                                                                                                                 ggAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGa                                >  4:194776/1‑149 (MQ=255)
                                                                                                                   agaATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACg                              <  6:58567/149‑1 (MQ=255)
                                                                                                                      aTGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGgcc                           <  8:25771/149‑1 (MQ=255)
                                                                                                                         gCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGgccgc                         <  5:400324/148‑1 (MQ=255)
                                                                                                                          cTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCt                       >  7:108342/1‑149 (MQ=255)
                                                                                                                          cTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCt                       >  5:170530/1‑149 (MQ=255)
                                                                                                                                 agcagcagcAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTgg                <  7:98056/149‑1 (MQ=255)
                                                                                                                                  gcagcagcaGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTggg               <  7:356264/149‑1 (MQ=255)
                                                                                                                                    agcagcagTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGTGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGgtg             >  7:373818/1‑149 (MQ=255)
                                                                                                                                         cagGTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGTCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTgg        <  4:106947/149‑1 (MQ=255)
                                                                                                                                            ttGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGTGa     <  6:398534/149‑1 (MQ=255)
                                                                                                                                            ttGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGCGa     >  1:181814/1‑149 (MQ=255)
                                                                                                                                            ttGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGCGTTTGGCGa     <  6:78570/149‑1 (MQ=255)
                                                                                                                                             cctggCCCGGGCGCTGGCGCCCGAGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGc       <  5:207986/143‑1 (MQ=255)
                                                                                                                                             cctggCCCGGGCGCTGGCGCCCGAGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGc       >  6:207986/4‑146 (MQ=255)
                                                                                                                                              ctggCCCGGGCGCTGGCGCCCGAGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGCGATa   >  5:403044/3‑149 (MQ=255)
                                                                                                                                               cGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGCGATAc  <  1:424559/149‑1 (MQ=255)
                                                                                                                                               cGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGGGGGTGTTTGGCGATAc  >  3:279015/1‑149 (MQ=255)
                                                                                                                                                  cTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGc                                                   >  4:356672/1‑97 (MQ=255)
                                                                                                                                                  cTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGc                                                   <  3:356672/97‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACGGCAAAGGCTTCTTCCTGGTCATGGGTGACCAGAATGGCGCTGGTACCGCGGCTTTTGAGAATGTCGCGAACCTCATGGCTCAGGCGCCGGCGCAGCTCCACGTCGAGGTTGGAGAATGGCTCGTCAAGCAGCAGCAGTTGCGGCTCGGGCGCCAGCGCCCGGGCCAGGGCGACACGTTGCTGCTGGCCGC‑CGGACAGCTCATGCGGGTAACGCCCGCCCAGCCCGCCGAGTTTGACCAGCTCCAGCATCTCTTCGATGACGGCCGCCTGGCGTGGGTGTTTGGCGATAC  >  NC_002947/1237501‑1237792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑