Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,243,738 T→A 8.4% intergenic (+27/+72) tsaA → / ← rnt peroxiredoxin/ribonuclease T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,243,7380TA 8.4% 261.8 / 8.9 93intergenic (+27/+72)tsaA/rntperoxiredoxin/ribonuclease T
Reads supporting (aligned to +/- strand):  ref base T (41/44);  new base A (4/4);  total (45/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGCTCAGCGGTTTTC  >  NC_002947/1243592‑1243885
                                                                                                                                                   |                                                                                                                                                      
cAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTc                                                                                                                                                      >  2:47618/1‑149 (MQ=255)
  gTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATAAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGt                                                                                                                                                    >  4:36473/1‑149 (MQ=255)
   ttCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTg                                                                                                                                                   <  7:258232/149‑1 (MQ=255)
   ttCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTg                                                                                                                                                   <  8:199152/149‑1 (MQ=255)
     ccACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGc                                                                                                                                                 >  4:29192/1‑149 (MQ=255)
      cACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGcc                                                                                                                                                <  7:393177/149‑1 (MQ=255)
      cACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGcc                                                                                                                                                >  8:188570/1‑149 (MQ=255)
       aCGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAAACCGGCCTTCGTGGcc                                                                                                                                                <  4:386770/149‑1 (MQ=255)
       aCGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCg                                                                                                                                               >  3:148525/1‑149 (MQ=255)
       aCGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCg                                                                                                                                               <  2:214189/149‑1 (MQ=255)
           agagCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑ttt                                                                                                                                          >  3:298837/1‑149 (MQ=255)
            gagCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCGGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCACGAAGGCCGG‑tttt                                                                                                                                         <  6:303874/149‑1 (MQ=255)
             agCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑ttttt                                                                                                                                        >  5:231142/1‑149 (MQ=255)
              gCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑tttttt                                                                                                                                       >  2:247845/1‑149 (MQ=255)
              gCACGGCGAAGGGGGCCCTGCCAACTGGAAAACAGGCGACAAGGGCATGAACGCGTCGCCTGAAGGCGGTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑tttttt                                                                                                                                       <  3:34576/149‑1 (MQ=255)
               cACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑tttttt                                                                                                                                       >  6:315553/1‑148 (MQ=255)
               cACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑tttttt                                                                                                                                       >  1:195683/1‑148 (MQ=255)
               cACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATA‑AAAAAACCGGCCACGAAGGCCGG‑ttttttc                                                                                                                                      <  1:419461/149‑2 (MQ=39)
                aCGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCg                                                                                                                                               <  3:49794/140‑1 (MQ=255)
                aCGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCg                                                                                                                                               >  4:49794/1‑140 (MQ=255)
                 cGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTAt                                                                                                                                    <  1:2504/149‑1 (MQ=255)
                 cGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTAt                                                                                                                                    <  6:437085/149‑1 (MQ=255)
                   gCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAAAGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATg                                                                                                                                   >  7:206200/1‑148 (MQ=255)
                       aGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTc                                                                                                                              <  1:99372/149‑1 (MQ=255)
                       aGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTc                                                                                                                              <  4:391456/149‑1 (MQ=255)
                         tgtgCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCt                                                                                                                            <  2:327585/149‑1 (MQ=255)
                              ccTGCCAACTGGAAAAAAGGCGACAAGGCCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGAAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGcc                                                                                                                       <  5:339678/149‑1 (MQ=255)
                               cTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑TAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCg                                                                                                                      <  7:257873/149‑1 (MQ=255)
                                  ccAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGcc                                                                                                                   >  7:335645/1‑149 (MQ=255)
                                    aaCTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTc                                                                                                                              <  2:368022/136‑1 (MQ=255)
                                    aaCTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTc                                                                                                                              >  1:368022/1‑136 (MQ=255)
                                    aaCTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTACATG‑AAAAAACCGGCCTTCCTGGACGG‑TTTTATTATGCCTTCCTGTGCCGTccac                                                                                                                 >  3:272033/1‑147 (MQ=255)
                                          aaaaaaGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTcgcg                                                                                                           <  4:148525/149‑1 (MQ=255)
                                           aaaaaGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCgg                                                                                                          <  2:396788/149‑1 (MQ=255)
                                           aaaaaGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCgg                                                                                                          <  8:58202/149‑1 (MQ=255)
                                                   gACAAGGGCATAAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑Gccc                                                                                                <  3:36473/149‑1 (MQ=255)
                                                    aCAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCcg                                                                                               >  6:49445/1‑149 (MQ=255)
                                                     cAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCcgc                                                                                              <  1:310368/149‑1 (MQ=255)
                                                         ggCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAAt                                                                                          <  1:422666/149‑1 (MQ=255)
                                                            aTGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑ttttttt                                                                                                                                      >  4:413528/1‑104 (MQ=255)
                                                            aTGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑ttttttt                                                                                                                                      <  3:413528/104‑1 (MQ=255)
                                                            aTGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGcc                                                                                       >  7:305269/1‑149 (MQ=255)
                                                              gAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCtt                                                                                     >  3:129118/1‑149 (MQ=255)
                                                               aaCGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCtt                                                                                     <  6:231142/148‑1 (MQ=255)
                                                                aCGCTTCGCCTGAAGGCGTTTCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTa                                                                                   <  4:73810/149‑1 (MQ=255)
                                                                aCGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTa                                                                                   <  6:129443/149‑1 (MQ=255)
                                                                 cGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAg                                                                                  <  3:182885/149‑1 (MQ=255)
                                                                       gCCTGAAGGCGTTGCTGCCTACCTGCGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATc                                                                            <  1:203086/149‑1 (MQ=255)
                                                                        ccTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATc                                                                            >  3:181567/1‑148 (MQ=255)
                                                                          tGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTATTTATGCCAACCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAAt                                                                         >  1:356262/1‑149 (MQ=255)
                                                                              ggCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGtt                                                                     >  4:167930/1‑149 (MQ=255)
                                                                               gCGTTGCTGCCTACCTGAGCTAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTg                                                                    <  1:247845/149‑1 (MQ=255)
                                                                                cGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTg                                                                    >  1:398707/1‑148 (MQ=255)
                                                                                 gTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAACCCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGaa                                                                  <  8:289195/149‑1 (MQ=255)
                                                                                 gTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGGTTTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTg                                                                    >  4:297052/1‑148 (MQ=255)
                                                                                   tgctgcCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGa                                                                   >  5:80531/1‑146 (MQ=255)
                                                                                   tgctgcCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGa                                                                   <  6:80531/146‑1 (MQ=255)
                                                                                       gcCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATcgcg                                                            >  5:136908/1‑149 (MQ=255)
                                                                                       gcCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATcgcg                                                            >  1:106326/1‑149 (MQ=255)
                                                                                        ccTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATcgc                                                             <  2:195683/147‑1 (MQ=255)
                                                                                           aCCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAg                                                        >  2:136947/1‑149 (MQ=255)
                                                                                              tGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAgccg                                                     >  2:39711/1‑149 (MQ=255)
                                                                                              tGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAgccg                                                     >  6:422786/1‑149 (MQ=255)
                                                                                                       aCGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAtttct                                            >  6:332688/1‑149 (MQ=255)
                                                                                                           tGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCAtttctttc                                         >  5:343273/1‑148 (MQ=255)
                                                                                                             gCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCa                                       <  5:422786/148‑1 (MQ=255)
                                                                                                               aaGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCg                                    <  4:181567/149‑1 (MQ=255)
                                                                                                                 gCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATcgc                                                             >  8:404987/1‑122 (MQ=255)
                                                                                                                 gCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATcgc                                                             <  7:404987/122‑1 (MQ=255)
                                                                                                                  cTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGtt                                 <  5:332688/149‑1 (MQ=255)
                                                                                                                     tAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATg                                                                                         >  4:7682/1‑90 (MQ=255)
                                                                                                                     tAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATg                                                                                         <  3:7682/90‑1 (MQ=255)
                                                                                                                     tAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGa                               <  5:49445/148‑1 (MQ=255)
                                                                                                                           ctgcGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGcc                        <  4:298837/149‑1 (MQ=255)
                                                                                                                           ctgcGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGcc                        <  1:47618/149‑1 (MQ=255)
                                                                                                                              cGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGcag                       >  2:114695/1‑145 (MQ=255)
                                                                                                                                 gCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGaa                  >  8:385392/1‑149 (MQ=255)
                                                                                                                                  cATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGaa                  <  4:129118/148‑1 (MQ=255)
                                                                                                                                  cATA‑AAAAAACCGGCCACGAAGGCCGG‑TTTTTTCATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCa                                       >  1:270062/1‑127 (MQ=38)
                                                                                                                                  cATA‑AAAAAACCGGCCACGAAGGCCGG‑TTTTTTCATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCa                                       <  2:270062/127‑1 (MQ=38)
                                                                                                                                  cATA‑AAAAAACCGGCCACGAAGGCCGG‑TTTTTTCATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGaa                  >  8:324327/1‑148 (MQ=38)
                                                                                                                                   aTG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACa                <  7:385392/149‑1 (MQ=255)
                                                                                                                                    ataaaaaaaCCGGCCACGAAGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGc                         >  1:341021/4‑140 (MQ=39)
                                                                                                                                    ataaaaaaaCCGGCCACGAAGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGc                         <  2:341021/137‑1 (MQ=39)
                                                                                                                                     g‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGCAGGCCCGGGAATGCCGTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGaa                  >  1:131320/1‑147 (MQ=255)
                                                                                                                                     g‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCTGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGc              >  3:310159/1‑149 (MQ=255)
                                                                                                                                     g‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAgg              >  5:133940/1‑148 (MQ=255)
                                                                                                                                        aaaaaCCGGCCACGAAGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTATTTCCAGCGGTTGACGATg                          >  2:365120/1‑135 (MQ=255)
                                                                                                                                          aaaCCGGCCTTCGTGGCCGGTTTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGCTCa           <  5:419776/149‑1 (MQ=255)
                                                                                                                                          aaaCCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGCTCAg          >  6:437416/1‑149 (MQ=255)
                                                                                                                                            cccGGCCTTCGTGGCCGG‑TTTTATTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGCTCAGCg        <  3:226170/148‑1 (MQ=255)
                                                                                                                                               ggCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGCTCAGCGGtt     <  7:176691/149‑1 (MQ=255)
                                                                                                                                                  cTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGCTCAGCGGTTTtc  >  2:266365/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
CAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATG‑AAAAAACCGGCCTTCGTGGCCGG‑TTTTTTTATGCCTTCCTGTGCCGGCCTCTTCGCGGGCGC‑‑GCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCGCAGAACAGCTCAGCGGTTTTC  >  NC_002947/1243592‑1243885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑