Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,245,704 C→A 6.4% P20Q (CCG→CAG)  PP_1087 → OmpA family outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,245,7040CA 6.4% 250.9 / 6.0 78P20Q (CCG→CAG) PP_1087OmpA family outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (37/36);  new base A (3/2);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01

CAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGTTTC  >  NC_002947/1245559‑1245850
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cAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTAGCCAGACTgccggc                                                                                                                                                 >  6:320513/1‑149 (MQ=255)
 aaTCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTgccggcc                                                                                                                                                >  4:142554/1‑149 (MQ=255)
 aaTCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTgccggcc                                                                                                                                                >  4:145709/1‑149 (MQ=255)
  aTCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTgccggccg                                                                                                                                               <  6:444649/149‑1 (MQ=255)
      tACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGCCAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGgcg                                                                                                                                            >  1:302993/1‑148 (MQ=255)
      tACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGgcg                                                                                                                                            >  5:224912/1‑148 (MQ=255)
        ccGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGGGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCAc                                                                                                                                         >  1:305902/1‑149 (MQ=255)
        ccGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCGGCCGGCAGGCTGGCGAAc                                                                                                                                         <  5:352818/149‑1 (MQ=255)
         cGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACt                                                                                                                                        >  1:44667/1‑149 (MQ=255)
          ggAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTg                                                                                                                                       <  1:351685/149‑1 (MQ=255)
           gAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCGGCCGGCAGGCTGGCGAAcagg                                                                                                                                      <  6:196793/149‑4 (MQ=255)
           gAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGa                                                                                                                                      >  3:96197/1‑149 (MQ=255)
               aCCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACttt                                                                                                                                  <  3:119482/149‑1 (MQ=255)
                ccccGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTc                                                                                                                                 >  2:351218/1‑149 (MQ=255)
                 cccGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTc                                                                                                                                 >  2:6140/1‑148 (MQ=255)
                   cGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGTCCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAg                                                                                                                              >  1:175461/1‑149 (MQ=255)
                   cGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAg                                                                                                                              <  5:361105/149‑1 (MQ=255)
                   cGGGGCGAGCACGGCGATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGGGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACGGCCttgc                                                                                                                                 >  2:54166/1‑144 (MQ=255)
                     gggCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGAc                                                                                                                            <  8:276066/149‑1 (MQ=255)
                             aCGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATgg                                                                                                                    <  3:145709/149‑1 (MQ=255)
                                     cAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACAtt                                                                                                            >  2:389594/1‑149 (MQ=255)
                                             ccGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGa                                                                                                     <  2:44667/148‑1 (MQ=255)
                                              cGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGaa                                                                                                    <  3:142554/148‑1 (MQ=255)
                                               ggACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCa                                                                                                                               <  5:355/120‑1 (MQ=255)
                                               ggACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCa                                                                                                                               >  6:355/1‑120 (MQ=255)
                                                gACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGt                                                                                                 <  3:380677/149‑1 (MQ=255)
                                                 aCAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTc                                                                                                <  1:370418/149‑1 (MQ=255)
                                                   aGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGa                                                                                              <  3:163429/149‑1 (MQ=255)
                                                     cGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAgg                                                                                            <  3:138658/149‑1 (MQ=255)
                                                     cGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAgg                                                                                            <  1:54166/149‑1 (MQ=255)
                                                     cGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAgg                                                                                            <  8:197617/149‑1 (MQ=255)
                                                     cGTCCGAAAGCCTGCTGGTAAGCCGTGAGAATTTGTGGAGCCGCCCGTGCGCCAGCCATACCTAGCCCAGTTGACGCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACAGTCCACACGCGGATGGAGCTCATTGCCTGGAAGGTCGAgg                                                                                            >  4:86619/1‑149 (MQ=255)
                                                       tCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGt                                                                                          <  2:302993/149‑1 (MQ=255)
                                                        ccGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGt                                                                                          >  7:120276/1‑148 (MQ=255)
                                                         cGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGa                                                                                        <  6:879/149‑1 (MQ=255)
                                                          gATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCATTGACTTTCCAGACGCGCATGGAGAACATTGCCt                                                                                                        >  4:162664/1‑132 (MQ=255)
                                                          gATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGAc                                                                                       >  4:344332/1‑149 (MQ=255)
                                                                   ctgGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGa                                                                               <  8:120276/148‑1 (MQ=255)
                                                                   ctgGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGa                                                                               <  1:351218/148‑1 (MQ=255)
                                                                   ctgGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAg                                                                              >  6:417875/1‑149 (MQ=255)
                                                                      gTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAgtgt                                                                           >  3:185978/1‑149 (MQ=255)
                                                                      gTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAgt                                                                             <  6:153021/147‑1 (MQ=255)
                                                                      gTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAgt                                                                             <  6:224912/147‑1 (MQ=255)
                                                                                aGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGagc                                                                  <  5:417875/148‑1 (MQ=255)
                                                                                aGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGagc                                                                  >  4:363458/1‑148 (MQ=255)
                                                                                   aTTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGagccagc                                                              <  6:211753/149‑1 (MQ=255)
                                                                                        tgGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGAt                                                          <  7:50022/148‑1 (MQ=255)
                                                                                          gAGCCGGCCGGGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGa                                                       <  8:115105/149‑1 (MQ=255)
                                                                                             ccgccCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGACTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAgg                                                    >  5:130134/1‑149 (MQ=255)
                                                                                                   gTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCgg                                              >  1:134776/1‑149 (MQ=255)
                                                                                                   gTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGATTCgg                                              >  1:323693/1‑149 (MQ=255)
                                                                                                      cgcCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCa                                            <  2:134776/148‑1 (MQ=255)
                                                                                                        ccAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCg                                         <  3:1736/149‑1 (MQ=255)
                                                                                                         cAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGa                                                                    >  6:143043/1‑121 (MQ=255)
                                                                                                         cAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCgg                                        >  4:290844/1‑149 (MQ=255)
                                                                                                         cAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCgg                                        >  8:244280/1‑149 (MQ=255)
                                                                                                                  cTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTAc                                     >  6:2932/1‑143 (MQ=255)
                                                                                                                  cTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTAc                                     <  5:2932/143‑1 (MQ=255)
                                                                                                                     gCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGc                             <  7:244280/148‑1 (MQ=255)
                                                                                                                     gCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGc                             <  1:6140/148‑1 (MQ=255)
                                                                                                                       ccTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTc                          >  3:224505/1‑149 (MQ=255)
                                                                                                                           ttGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGc                      <  3:363458/149‑1 (MQ=255)
                                                                                                                              aCCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGAc                                                                                       <  4:77489/81‑1 (MQ=255)
                                                                                                                              aCCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCgg                        <  5:267427/144‑1 (MQ=255)
                                                                                                                              aCCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCgg                        >  6:267427/1‑144 (MQ=255)
                                                                                                                               gtcagtgcGCCGGCCGGCAGGCTGGCGAACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGc                  >  2:148040/8‑149 (MQ=255)
                                                                                                                               cccTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGc                  >  5:286438/1‑149 (MQ=255)
                                                                                                                                ccTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGc                  >  7:211513/1‑148 (MQ=255)
                                                                                                                                ccTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGACGATCGAAGGTTTCGGAAGCGGTACGTTCGTGCGTCGGGCAGGCg                 >  8:404880/1‑149 (MQ=255)
                                                                                                                                 cTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGa                <  5:155505/149‑1 (MQ=255)
                                                                                                                                   gTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCTGTTAGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGag               >  7:297220/1‑148 (MQ=255)
                                                                                                                                    ttCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGag               >  5:425032/1‑147 (MQ=255)
                                                                                                                                    ttCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGag               <  2:47679/147‑1 (MQ=255)
                                                                                                                                      cGCCGGCCGGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGagcagc           >  1:292604/1‑149 (MQ=255)
                                                                                                                                      cGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGagcagc           >  2:372676/1‑149 (MQ=255)
                                                                                                                                       gCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGTGGTACGTTCGTGCGTCGGGCAGGCGAGCAGcc          >  5:424090/1‑149 (MQ=255)
                                                                                                                                       gCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGagcagc           <  1:372676/148‑1 (MQ=255)
                                                                                                                                       gCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGcc          >  5:338511/1‑149 (MQ=255)
                                                                                                                                        ccggCCGGCAGGCTGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCg         >  1:397/4‑149 (MQ=255)
                                                                                                                                        ccAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCg         >  1:194016/1‑149 (MQ=255)
                                                                                                                                          aGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGt                               >  1:15199/1‑125 (MQ=255)
                                                                                                                                          aGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGt                               <  2:15199/125‑1 (MQ=255)
                                                                                                                                                ccggccggCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGttt   <  6:425032/147‑1 (MQ=255)
                                                                                                                                                ccggccggCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGTTTc  <  4:224505/148‑1 (MQ=255)
                                                                                                                                                ccggccggCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGTTTc  <  1:389594/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CAATCCTACCGGAAAACCCCGGGGCGAGCACGGCTATCAAGTTTTCCGGACAGCGTCCGATAGCCTGCTGGTAAGCCGTCAGAATTTGTGGAGCCGCCCGTGCGCCAGCGTTACCTAGCCCTGTTGACCCTGTTCGCCAGCCTGCCGGCCGGCGCACTGACTTTCCAGACGCGCATGGAGAACATTGCCTGGAAGGTCGAGGGTGACCAGTTCGAGTGTCGTCTGAGCCAGCCGATCGAAGGTTTCGGCAGCGGTACGTTCGTGCGTCGGGCAGGCGAGCAGCCGGTGTTTC  >  NC_002947/1245559‑1245850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: