Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,306,918	G→C	7.0%	R234G (CGC→GGC) ‡	livK ←	branched‑chain amino acids ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,306,918	0	G	C	7.0%	276.3 / 9.3	89	R234G (CGC→GGC) ‡	livK	branched‑chain amino acids ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (42/39);  new base C (2/4);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01

TCTTGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCTTTGCTTTCCAGG  >  NC_002947/1306771‑1307066                                                                                                                                                    |                                                                                                                                                        tcttGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGgcg                                                                                                                                                         >  8:217742/1‑148 (MQ=255)   ttGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAg                                                                                                                                                      >  2:351155/1‑149 (MQ=255)     gTTCGCCGGGTCCGCGTCGAACGACTTCGTCATGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAgg                                                                                                                                                     <  7:184885/148‑1 (MQ=255)     gTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCa                                                                                                                                                       <  3:188100/146‑1 (MQ=255)     gTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAgg                                                                                                                                                     <  2:376445/148‑1 (MQ=255)     gTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑a                                                                                                                                                   >  6:398930/1‑149 (MQ=255)        cGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑At                                                                                                                                                  >  1:287640/1‑147 (MQ=255)         gCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAg                                                                                                                                               >  8:51068/1‑149 (MQ=255)          ccGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGATCCTGCGCCAGG‑CTCAgg                                                                                                                                              <  3:285757/149‑1 (MQ=255)          ccGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGATCCTGCGCCAGG‑CTCAgg                                                                                                                                              <  5:99490/149‑1 (MQ=255)             ggTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGc‑c                                                                                                                                           >  4:111313/1‑148 (MQ=255)               tCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAg                                                                                                                                        <  2:332522/149‑1 (MQ=255)                ccGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGc                                                                                                                                       >  3:84686/1‑149 (MQ=255)                    gTCGAACGACTTCGGCAGGGTGACCCGCAGGCCTTCTGCGGCGTTCGGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAgg                                                                                                                                              <  6:118357/139‑1 (MQ=255)                    gTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTGTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAgg                                                                                                                                              >  5:118357/1‑139 (MQ=255)                        aaCGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGggtg                                                                                                                               >  3:68922/1‑149 (MQ=255)                        aaCGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGggtg                                                                                                                               <  8:18432/149‑1 (MQ=255)                        aaCGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGggt                                                                                                                                >  4:336078/1‑148 (MQ=255)                        aaCGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGggt                                                                                                                                <  3:336078/148‑1 (MQ=255)                          cGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGggtggt                                                                                                                             <  5:27061/149‑1 (MQ=255)                           gACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGggtgg                                                                                                                              >  2:432883/1‑147 (MQ=255)                            aCTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAg                                                                                                                           >  3:139320/1‑149 (MQ=255)                                cGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAgccg                                                                                                                        <  6:420792/148‑1 (MQ=255)                                      ggTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCgtagt                                                                                                                 <  5:341905/149‑1 (MQ=255)                                      ggTGACCAGCAGGCCTTCGGAGGCGTTCAGGGTGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCgtag                                                                                                                  >  1:248317/1‑148 (MQ=255)                                          aCCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGAc                                                                                                             >  4:111434/1‑149 (MQ=255)                                            cagcagGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACg                                                                                                            >  1:49493/1‑148 (MQ=255)                                            cagcagGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACg                                                                                                            >  4:185210/1‑148 (MQ=255)                                             agcagGCCTTCGGAGGCGTTCTGGGTGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGaa                                                                                                          <  2:248317/149‑1 (MQ=255)                                             agcagGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACg                                                                                                            >  3:294089/1‑147 (MQ=255)                                             agcagGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGaa                                                                                                          <  2:287640/149‑1 (MQ=255)                                              tcagGCCTTCGGTGGAGGTCTGGTGGATCTGTGAAATGTAGTGGTTAGCCAGGGGTTCCGGACCCTTGAACTTGGCTTTCAGTCCTTTTTCCTGATCCTGGCGCAGT‑ATTAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAg                                                                                                         <  4:139320/148‑1 (MQ=255)                                              gcagGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAg                                                                                                         <  1:351155/149‑1 (MQ=255)                                                agGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTc                                                                                                       <  1:172041/149‑1 (MQ=255)                                                agGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTc                                                                                                       <  2:49493/149‑1 (MQ=255)                                                    cTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACg                                                                                                   <  3:185210/149‑1 (MQ=255)                                                    cTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CAAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACg                                                                                                   >  6:49945/1‑149 (MQ=255)                                                       cGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACgttg                                                                                                >  8:278380/1‑149 (MQ=255)                                                       cGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACgt                                                                                                  <  1:361320/147‑1 (MQ=255)                                                       cGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACgt                                                                                                  <  4:68922/147‑1 (MQ=255)                                                           ggCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑Ct                                                                                           <  8:356452/149‑1 (MQ=255)                                                                 cTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGCTTCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCtt                                                                                       <  1:107780/148‑1 (MQ=255)                                                                           tGCGAAATGGAGTTGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGgat                                                                           >  2:181916/1‑149 (MQ=255)                                                                             cGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCt                                                                               <  1:432883/143‑1 (MQ=255)                                                                             cGAAATCGAGTCGGTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGga                                                                            <  6:12711/146‑1 (MQ=255)                                                                                 aTGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCga                                                                     <  8:222060/149‑1 (MQ=255)                                                                                 aTGGAGTCGTTACCCACGCCTGCCGGACCGATGAACTTGGCTTTCATGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCga                                                                     <  7:350901/149‑1 (MQ=255)                                                                                  tGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCgag                                                                    <  4:84686/149‑1 (MQ=255)                                                                                    gcgTCGTTACCCACGCCTTCCTGACACATCAACATGGCTTTCAGTCCTTTTTCCTCAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTCGCCGCCGTAGTAGACCAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCgagga                                                                  <  8:132924/147‑1 (MQ=255)                                                                                    gAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCgag                                                                    >  3:387900/1‑147 (MQ=255)                                                                                     aGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCgaggag                                                                 >  2:141056/1‑149 (MQ=255)                                                                                     aGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCgag                                                                    >  7:319012/1‑146 (MQ=255)                                                                                         gTTACCCACGGCTTCCGGACCCATGAACTTTGCTTTCACGCCTTTTTCCTGATGCTTGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTATTAGACGAAGTCCACGTTGTTCCTGCTTGAGCTTCTGGATGATCGAGGAGaa                                                               <  1:260655/148‑1 (MQ=255)                                                                                          ttACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAggc                                                            <  7:278380/149‑3 (MQ=255)                                                                                           tACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTc                                                            <  1:181916/148‑1 (MQ=255)                                                                                             cccACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCtt                                                         <  7:51068/149‑1 (MQ=255)                                                                                             cccACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCtt                                                         <  4:294089/149‑1 (MQ=255)                                                                                             cccACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCtt                                                         <  3:111313/149‑1 (MQ=255)                                                                                                aCGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGt                                                       >  3:241565/1‑148 (MQ=255)                                                                                                   ccTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTGGACGTTGTT‑GTGCTTGGGCTGCGGGAGGATCGAGGAGATGTCCTTGTCAcg                                                   >  2:335886/1‑148 (MQ=255)                                                                                                    cTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCAccc                                                  >  6:249747/1‑149 (MQ=255)                                                                                                      tCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCcgc                                                >  4:11219/1‑149 (MQ=255)                                                                                                       ccGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCcgcg                                               >  5:121718/1‑149 (MQ=255)                                                                                                               aTGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGcc                                       >  7:190086/1‑149 (MQ=255)                                                                                                               aTGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGcc                                       >  4:183285/1‑149 (MQ=255)                                                                                                               aTGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGcc                                       >  2:73902/1‑149 (MQ=255)                                                                                                               aTGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGACGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCATTGTCACAAGCGTTCAGGca                                       >  3:237097/1‑148 (MQ=255)                                                                                                                tGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCt                                      <  4:412211/149‑1 (MQ=255)                                                                                                                    cTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCt                                      <  3:162871/145‑1 (MQ=255)                                                                                                                    cTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCt                                      >  4:162871/1‑145 (MQ=255)                                                                                                                     ttGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGaa                                 >  5:334366/1‑149 (MQ=255)                                                                                                                            tCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGc                           <  4:214866/148‑1 (MQ=255)                                                                                                                            tCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGc                           >  4:255337/1‑148 (MQ=255)                                                                                                                              aGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAAc                        >  1:417136/1‑149 (MQ=255)                                                                                                                                gCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCt                      >  7:270419/1‑149 (MQ=255)                                                                                                                                 gcctgatCCTGCGCCAGGCTCAGGAAAAAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACg                             >  6:61514/7‑142 (MQ=255)                                                                                                                                 gcctgatCCTGCGCCAGGCTCAGGAAAAAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACg                             <  5:61514/136‑1 (MQ=255)                                                                                                                                   tttttCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTgg                   >  3:368072/1‑149 (MQ=255)                                                                                                                                   tttttCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTgg                   >  5:256811/1‑149 (MQ=255)                                                                                                                                    ttttCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTc                                            >  6:316481/1‑123 (MQ=255)                                                                                                                                    ttttCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTc                                            <  5:316481/123‑1 (MQ=255)                                                                                                                                     tttCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGt                  <  6:334366/148‑1 (MQ=255)                                                                                                                                        ccTGATCCTGCGCCAGG‑CTCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCt                                      <  6:215231/125‑1 (MQ=255)                                                                                                                                        ccTGATCCTGCGCCAGG‑CTCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCt                                      <  6:209395/125‑1 (MQ=255)                                                                                                                                        ccTGATCCTGCGCCAGG‑CTCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCt                                      >  5:215231/1‑125 (MQ=255)                                                                                                                                        ccTGATCCTGCGCCAGG‑CTCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCt                                      >  5:209395/1‑125 (MQ=255)                                                                                                                                        ccTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCt              >  1:426184/1‑149 (MQ=255)                                                                                                                                          tGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTCGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTACTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCttt            >  8:193925/1‑149 (MQ=255)                                                                                                                                           gAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCTTTg           >  4:259318/1‑149 (MQ=255)                                                                                                                                            aGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCTTTGc          <  6:256811/149‑1 (MQ=255)                                                                                                                                                    gcAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCTTTGCTTTCCAgg  <  8:190086/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                        TCTTGTTCGCCGGGTCCGCGTCGAACGACTTCGGCAGGGTGACCAGCAGGCCTTCGGAGGCGTTCTGGGCGATCTGCGAAATGGAGTCGTTACCCACGCCTTCCGGACCCATGAACTTGGCTTTCAGGCCTTTTTCCTGAGCCTGGCGCAGG‑ATCAGGC‑CCAGCTCTGGGTGGTAGCCGCCGTAGTAGACGAAGTCGACGTTGTT‑CTGCTTGAGCTTCTGGATGATCGAGGAGAAGTCCTTGTCACCCGCGTTCAGGCCTTCGAACACGGCAACCTTGGTGCCTTTGCTTTCCAGG  >  NC_002947/1306771‑1307066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑