Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,350,501	T→A	9.3%	K11N (AAA→AAT) ‡	PP_1176 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,350,501	0	T	A	9.3%	208.6 / 10.8	75	K11N (AAA→AAT) ‡	PP_1176	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (34/34);  new base A (4/3);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

AGCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGT  >  NC_002947/1350357‑1350647                                                                                                                                                 |                                                                                                                                                    agCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTgg                                                                                                                                                 <  1:178511/149‑1 (MQ=255)         cTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCAGGAAACCGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGCCtt                                                                                                                                          <  2:269175/148‑1 (MQ=255)           gACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                       >  2:381628/1‑149 (MQ=255)            aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                       <  4:151729/148‑1 (MQ=255)            aCGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgt                                                                                                                                        <  2:382721/147‑1 (MQ=255)              gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGAt                                                                                                                                    >  3:43268/1‑149 (MQ=255)              gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGCCtttttatt                                                                                                                                    <  5:388590/149‑4 (MQ=255)                      gATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGcc                                                                                                                            >  4:31113/1‑149 (MQ=255)                        ttCGGCTATGTGAGCGTTCTTAAGCGCCGTAAAACGTTATCGCGATGTCCAAAAACACGCAAAGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCg                                                                                                                          <  1:45058/149‑1 (MQ=255)                        ttCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCg                                                                                                                          >  5:138014/1‑149 (MQ=255)                           cGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGt                                                                                                                        >  8:55078/1‑148 (MQ=255)                                agtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                    <  8:156672/146‑1 (MQ=255)                                 gtgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat‑caa                                                                                                                >  5:193725/1‑149 (MQ=255)                                  tgAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTcaat‑caat                                                                                                               <  7:152577/149‑1 (MQ=255)                                       gTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAg                                                                                                          >  3:133453/1‑149 (MQ=255)                                         tCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCt                                                                                                        >  6:324380/1‑149 (MQ=255)                                         tCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCt                                                                                                        >  5:340114/1‑149 (MQ=255)                                            tAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTACCAGGAAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCACAGCTCaa                                                                                                     >  7:395743/1‑149 (MQ=255)                                             aaGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCaa                                                                                                     <  2:242417/148‑1 (MQ=255)                                                cgcCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGcc                                                                                                 <  8:209092/149‑1 (MQ=255)                                                cgcCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGcc                                                                                                 <  6:340114/149‑1 (MQ=255)                                                cgcCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGAAATAAAAATGCTGACATTTTGCAGCCTTGTGGATGCTCAGACCGGTCAAT‑CAATCCCAGATCAACGcc                                                                                                 >  4:85772/1‑149 (MQ=255)                                                 gacGGAAAACGTTATCGCGATGTCCAAAATCAGGCAATGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGTTCAAT‑CAATCCCAGCTCAACGccc                                                                                                <  4:242345/147‑1 (MQ=255)                                                   cGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGATTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCa                                                                                              >  5:61663/1‑149 (MQ=255)                                                    ggAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCAc                                                                                             >  1:76519/1‑149 (MQ=255)                                                        aaCGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGt                                                                                         <  4:43268/149‑1 (MQ=255)                                                         aCGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTc                                                                                        <  1:402759/149‑1 (MQ=255)                                                           gTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTg                                                                                      <  5:324380/149‑1 (MQ=255)                                                                   gATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGa                                                                              <  6:193725/149‑1 (MQ=255)                                                                   gATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGa                                                                              <  6:138014/149‑1 (MQ=255)                                                                        ccAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATcaca                                                                         >  4:102479/1‑149 (MQ=255)                                                                         cAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCacac                                                                        >  3:396211/1‑149 (MQ=255)                                                                         cAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCacac                                                                        >  6:137646/1‑149 (MQ=255)                                                                          aaaaaCAGGCAAGTTCATCAGCCGTAGGAAACGTCCTGTTCTGCTTCTCCAAGGCAAAAAAAAGGCTGCCATTAGGCACCATTGTTGATGCTCAGACCGGTCAAT‑CAATCACAGCTCAACGAAACACCACTCTGATAAGCGATCACAcg                                                                       >  2:185846/1‑149 (MQ=255)                                                                          aaaaaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCCCAcg                                                                       >  3:266108/1‑149 (MQ=255)                                                                           aaaaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACAcgc                                                                      <  1:34728/149‑1 (MQ=255)                                                                             aaCAGGCTAGGTCGTCTGCCGTAGGAAACGTCCTGTTCTGCTTCTTCCATGCAATAAAAAGGCTGCCATTTTGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCAACCCGTCTGATACTCGATCAGACGCGt                                                                    <  4:133453/149‑1 (MQ=255)                                                                                atgCAAGGGCGGCAGCCTTATGAAAGGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCACGGTCAAT‑CAATCCCAGCTCATCGCCCCACCCGTCTGATACTCGATCACACGCGtctc                                                                 <  3:85772/147‑1 (MQ=255)                                                                                 ggCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCa                                                                <  3:31113/149‑1 (MQ=255)                                                                                  gCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCa                                                                >  7:372393/1‑148 (MQ=255)                                                                                   cAAGGTCGGCAGCCGTACGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATCATCGTTCACGCGCGTCTCaac                                                              >  8:388198/1‑148 (MQ=255)                                                                                    aaGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCa                                                                >  5:247167/1‑146 (MQ=255)                                                                                     aGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAaaga                                                            <  1:104245/149‑1 (MQ=255)                                                                                       gTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAaagaag                                                          <  3:102479/149‑1 (MQ=255)                                                                                          ggCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGttc                                                       <  4:396211/149‑1 (MQ=255)                                                                                              gCCGTAGGAAACGTCCTGTTCTGCGTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTtcttct                                                   <  8:297009/149‑1 (MQ=255)                                                                                              gCCGTAGGAAACGTCCTGTGCTGCTTCTTCCAGTCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCAGGTCAAT‑CAATCCCATCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTtcttct                                                   <  7:289537/149‑1 (MQ=255)                                                                                               ccGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTtctc                                                  >  6:347495/1‑149 (MQ=255)                                                                                               ccGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTtctc                                                  <  7:55078/149‑1 (MQ=255)                                                                                               ccGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTtctc                                                  >  6:47554/1‑149 (MQ=255)                                                                                                      aaaCGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCttcttc                                           <  5:137646/149‑1 (MQ=255)                                                                                                          gTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGt                                       <  2:76519/149‑1 (MQ=255)                                                                                                             cTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTcc                                     <  5:47554/148‑1 (MQ=255)                                                                                                               gTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCAt                                   >  2:113669/1‑148 (MQ=255)                                                                                                                ttctgcttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGa                                 >  2:34442/1‑149 (MQ=255)                                                                                                                     cttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGAtct                              <  6:247167/147‑1 (MQ=255)                                                                                                                            cAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATc                      >  8:143045/1‑148 (MQ=255)                                                                                                                               gCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATg                  >  2:422140/1‑149 (MQ=255)                                                                                                                               gCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATg                  >  3:73128/1‑149 (MQ=255)                                                                                                                               gCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATg                  >  1:400931/1‑149 (MQ=255)                                                                                                                                    aacaaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATg                                  >  3:138138/4‑128 (MQ=255)                                                                                                                                    aacaaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATg                                  <  4:138138/125‑1 (MQ=255)                                                                                                                                    aaaaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCCAATCCCAACTCAACGCCCCACCCGTc                                                                                        <  1:226648/75‑1 (MQ=255)                                                                                                                                    aaaaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAAc             >  3:183374/1‑149 (MQ=255)                                                                                                                                    aaaaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAACTCAACGCCCCACCCGTc                                                                                        >  2:226648/1‑74 (MQ=255)                                                                                                                                     acaaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCAATGATCTCGCTCATCCATGGGAACg            >  6:262740/3‑149 (MQ=255)                                                                                                                                      caaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACgg           >  6:423862/2‑149 (MQ=255)                                                                                                                                      caaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACgg           >  5:131765/2‑149 (MQ=255)                                                                                                                                      aaaGGCTGCCATTTGACAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACgg           >  4:356208/1‑149 (MQ=255)                                                                                                                                              ccATTTTGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CACTCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTa    <  8:395743/148‑1 (MQ=255)                                                                                                                                              ccATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTa    <  6:61663/148‑1 (MQ=255)                                                                                                                                              ccATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTCCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTa    <  1:113669/148‑1 (MQ=255)                                                                                                                                               cATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCGACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                 <  2:179923/134‑1 (MQ=255)                                                                                                                                               cATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATgg                 >  1:179923/1‑134 (MQ=255)                                                                                                                                               cATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGAGCACACGCGTCTCAAACAAGTTGTTCTCGTGCTTCAAGTCCATGATCTGGCTCATCCATGCGAACAGATTGTTAgg  >  8:361466/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                                    AGCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAT‑CAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGT  >  NC_002947/1350357‑1350647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑