Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,382,326 A→T 7.1% intergenic (+12/+15) dinB → / ← PP_1204 DNA polymerase IV/lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,382,3260AT 7.1% 211.3 / 5.5 70intergenic (+12/+15)dinB/PP_1204DNA polymerase IV/lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (34/31);  new base T (2/3);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CCTGGAGAGTTATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCT  >  NC_002947/1382179‑1382470
                                                                                                                                                    |                                                                                                                                                
ccTGGAGAGTTATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAAt                                                                                                                                                 <  3:70065/149‑1 (MQ=255)
 cTGGAGAGTTATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATg                                                                                                                                                >  1:287375/1‑149 (MQ=255)
 cTGGAGAGTTATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATg                                                                                                                                                >  8:297567/1‑149 (MQ=255)
    gagaGTTATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAg                                                                                                                                             >  3:25979/1‑149 (MQ=255)
         ttATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGGAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCt                                                                                                                                        >  5:303454/1‑149 (MQ=255)
           aTCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCATTGCAGCCCCtt                                                                                                                                       <  6:363346/148‑1 (MQ=255)
               gCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCACTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTAt                                                                                                                                   >  5:421010/1‑148 (MQ=255)
                      cTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCATTGCAGCCCCttt                                                                                                                                      <  3:337817/138‑2 (MQ=255)
                      cTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCATTGCAGCCCCttt                                                                                                                                      >  4:337817/1‑137 (MQ=255)
                          ggCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCt                                                                                                                       <  7:297567/149‑1 (MQ=255)
                           gCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTg                                                                                                                      >  5:177645/1‑149 (MQ=255)
                            cAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTg                                                                                                                      >  3:195678/1‑148 (MQ=255)
                              ggCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTGTCCGCCCAAATGAAAGGGGCTGCAATGCAGAGCCTTCTATTAATGAACTCCTGGAt                                                                                                                   >  3:302272/1‑149 (MQ=255)
                               gCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATc                                                                                                                  <  4:338823/149‑1 (MQ=255)
                                  ttCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGTGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGc                                                                                                               <  4:307868/149‑1 (MQ=255)
                                       ccGTGGAGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGTCTGCAATGCAGCCCCTTCTATTAATGCAAGCATGGATACGccacc                                                                                                           >  7:267887/1‑148 (MQ=255)
                                         gTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAAc                                                                                                        >  7:121884/1‑149 (MQ=255)
                                          tGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAAcc                                                                                                       >  6:388721/1‑149 (MQ=255)
                                                 aaGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCATTGCAGCCCCtt                                                                                                                                       <  3:349547/110‑1 (MQ=255)
                                                 aaGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCATTGCAGCCCCtt                                                                                                                                       >  4:349547/1‑110 (MQ=255)
                                                 aaGCCGGAGCGCTTGCTAGCGCTAGGGGTGAGGTTGCGTGACTTGCGCGGCGCGC‑ATGAACAGTTGGAGCACTAACCGCCCAAATCAAAGGGGCTGCAATGCCGCAACTTCTATTAATGCACGCCAGGATCCGCAACCAACCGGCCAGt                                                                                                >  4:126664/1‑149 (MQ=255)
                                                   gCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTgg                                                                                              <  2:300900/149‑1 (MQ=255)
                                                    ccGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGc                                                                                             >  4:295151/1‑149 (MQ=255)
                                                     cGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGA‑ATGAACAGTTGTAGCTGTTTCCGCCCAAATGAAAGTGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGcc                                                                                            <  8:344158/149‑1 (MQ=255)
                                                         gcgcTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTgg                                                                                        <  4:25979/149‑1 (MQ=255)
                                                          cgcTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTgg                                                                                        <  4:311580/148‑1 (MQ=255)
                                                           gcTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGc                                                                                             >  7:44835/1‑142 (MQ=255)
                                                           gcTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGc                                                                                             <  8:44835/142‑1 (MQ=255)
                                                           gcTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGt                                                                                       >  7:109906/1‑148 (MQ=255)
                                                            cTTGCTAGGTGTATGTGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTaa                                                                                     <  6:303454/149‑1 (MQ=255)
                                                                   ggggtaggggtGAGGGTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGAc                                                                                <  8:331845/147‑1 (MQ=255)
                                                                    gggtaggggtGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGc                                                                             <  5:350568/149‑1 (MQ=255)
                                                                     ggtaggggtGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCa                                                                            <  4:195678/149‑1 (MQ=255)
                                                                      gtaggggtGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAg                                                                           >  8:17851/1‑149 (MQ=255)
                                                                      gtaggggtGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAg                                                                           <  2:89674/149‑1 (MQ=255)
                                                                         ggggtGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGaa                                                                        <  7:17851/149‑1 (MQ=255)
                                                                            gtGAGGTTGCGTGACTTGCGGGGCGCGCGATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCa                                                                            >  1:247310/1‑143 (MQ=255)
                                                                            gtGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGTCTTgg                                                                                        >  8:21322/1‑130 (MQ=255)
                                                                            gtGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGTCTTgg                                                                                        <  7:21322/130‑1 (MQ=255)
                                                                            gtGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCa                                                                            <  2:247310/142‑1 (MQ=255)
                                                                             tGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGc                                                                                             >  4:253479/1‑124 (MQ=255)
                                                                             tGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGc                                                                                             <  3:253479/124‑1 (MQ=255)
                                                                                ggTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCctgc                                                                 <  6:177645/149‑1 (MQ=255)
                                                                                ggTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCctgc                                                                 <  3:295151/149‑1 (MQ=255)
                                                                                 gTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCctgct                                                                <  5:388721/149‑1 (MQ=255)
                                                                                    gCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCtgctgc                                                              <  2:201708/148‑1 (MQ=255)
                                                                                      gTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGc                                                           >  3:119851/1‑149 (MQ=255)
                                                                                                 ggCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGTAACTCCTGCTGCAGCTCCGGATCGtt                                                <  4:119851/149‑1 (MQ=255)
                                                                                                 ggCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGtt                                                <  6:421010/149‑1 (MQ=255)
                                                                                                     ggc‑ATGAAGAGTTGGATCTGTTTCCGCCAAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCATGAACGCCTGCTGCAGCTCCTGATCGTTgggg                                            <  8:267887/148‑1 (MQ=255)
                                                                                                     cgc‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCgg                                            <  2:287375/149‑1 (MQ=255)
                                                                                                           gAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAg                                       >  7:164642/1‑149 (MQ=255)
                                                                                                            aaCAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGc                                      >  3:300667/1‑149 (MQ=255)
                                                                                                             aCAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGTCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCt                                     >  5:351933/1‑149 (MQ=255)
                                                                                                             aCAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCt                                     >  3:427708/1‑149 (MQ=255)
                                                                                                                   ggAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGAt                                 >  2:246988/1‑147 (MQ=255)
                                                                                                                    gAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAg                              >  5:57767/1‑149 (MQ=255)
                                                                                                                         gTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCgg                                            >  7:130363/1‑130 (MQ=255)
                                                                                                                         gTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCgg                                            <  8:130363/130‑1 (MQ=255)
                                                                                                                          tttCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTg                        >  5:420020/1‑149 (MQ=255)
                                                                                                                          tttCCACCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGc                                              <  2:178578/127‑1 (MQ=255)
                                                                                                                          tttCCACCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGc                                              >  1:178578/1‑127 (MQ=255)
                                                                                                                               gCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCa                   <  8:109906/149‑1 (MQ=255)
                                                                                                                                   aaaTGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTaa                                                                                     >  8:63861/1‑79 (MQ=255)
                                                                                                                                   aaaTGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTaa                                                                                     <  7:63861/79‑1 (MQ=255)
                                                                                                                                   aaaTGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCAttt                >  6:129169/1‑148 (MQ=255)
                                                                                                                                    aaTGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCa              <  4:300667/149‑1 (MQ=255)
                                                                                                                                        aaaGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGGCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTgg          >  2:262425/1‑149 (MQ=255)
                                                                                                                                          aGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCt        >  3:43968/1‑149 (MQ=255)
                                                                                                                                                 gCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCctct  >  5:220900/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CCTGGAGAGTTATCGGCAATTGCTGGGGCAGGCGTTCGCCCGTGGCGGCAAGCCGGTGCGCTTGCTAGGGGTAGGGGTGAGGTTGCGTGACTTGCGGGGCGCGC‑ATGAACAGTTGGAGCTGTTTCCGCCCAAATGAAAGGGGCTGCAATGCAGCCCCTTCTATTAATGCACGCCTGGATCCGCCACCAACCGGCCAGTGGCCTTGGTAAGCGACTGCAGGAACTCCTGCTGCAGCTCCGGATCGTTGCGGGTCAGCTCGATCAGGCTCTGTTCCATTTCACTGGCTTCCTCT  >  NC_002947/1382179‑1382470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑