Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,400,419 G→C 5.5% G130A (GGT→GCT)  ybgF → TolQRA transport system periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,400,4190GC 5.5% 275.9 / 4.2 85G130A (GGT→GCT) ybgFTolQRA transport system periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (41/39);  new base C (2/3);  total (43/42)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGATGTGTCCCGCATGAAGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGCGTTCAACGCCTTCCTGCGCAAGTACCC  >  NC_002947/1400274‑1400566
                                                                                                                                                 |                                                                                                                                                   
aaCGATGTGTCCCGCATGAAGCAGGAAAACCTGGAGCGTTACCAGGACCAGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCCATGCCGCAGCAGGtgc                                                                                                                                                  >  1:413818/1‑149 (MQ=255)
     tgtgTCCCGCATGAAGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGtgctgctg                                                                                                                                             <  4:35906/149‑1 (MQ=255)
       tgtCCCGCATGAAGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTgcg                                                                                                                                           <  5:142136/149‑1 (MQ=255)
       tgtCCCGCATGAAGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGCACCTGCTgcg                                                                                                                                           <  6:420292/149‑1 (MQ=255)
        gtCCCGCATGAAGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGCACCTGCTgcg                                                                                                                                           <  2:168368/148‑1 (MQ=255)
        ggcccGCATGAAGGAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTgcgc                                                                                                                                          <  4:320586/147‑1 (MQ=255)
         tCCCGCATGAAGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTgcgc                                                                                                                                          >  8:33122/1‑148 (MQ=255)
         tCCCGCATGAAGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTgcgc                                                                                                                                          >  5:406946/1‑148 (MQ=255)
         tCCCGCATGAAGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCACCtgctgcggcat                                                                                                                                          <  8:207345/148‑3 (MQ=255)
         tCCCGCATGAAGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGCTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGCTGCTGCTccgc                                                                                                                                          >  8:26743/1‑148 (MQ=255)
              cATGAAGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCaacaac                                                                                                                                    <  7:389234/149‑1 (MQ=255)
                  caGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAAccgg                                                                                                                                 <  1:158630/147‑1 (MQ=255)
                  aaGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAAccggc                                                                                                                                <  7:19186/149‑1 (MQ=255)
                  aaGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAAccggc                                                                                                                                >  6:194678/1‑149 (MQ=255)
                  aaGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAAccggc                                                                                                                                >  2:34382/1‑149 (MQ=255)
                    gCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCTTCATCAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAAccggccg                                                                                                                              <  1:362373/149‑1 (MQ=255)
                     cAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAAccggccgg                                                                                                                             <  5:244173/149‑1 (MQ=255)
                      aGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAAccggccgg                                                                                                                             >  3:381362/1‑148 (MQ=255)
                       ggAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAAccggccgg                                                                                                                             >  1:265564/1‑147 (MQ=255)
                        gAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAg                                                                                                                          >  6:5331/1‑149 (MQ=255)
                          aaaCCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTa                                                                                                                        <  5:280200/149‑1 (MQ=255)
                             ccTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTagcc                                                                                                                     >  1:248747/1‑149 (MQ=255)
                              cTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTagc                                                                                                                      >  4:430070/1‑147 (MQ=255)
                                    cGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCgg                                                                                                              <  4:117877/149‑1 (MQ=255)
                                         ccAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATc                                                                                                         >  5:16513/1‑149 (MQ=255)
                                         ccAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATc                                                                                                         >  1:79441/1‑149 (MQ=255)
                                          cAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATcc                                                                                                        <  2:358231/149‑1 (MQ=255)
                                           aGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCg                                                                                                       <  5:194678/149‑1 (MQ=255)
                                           aGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCg                                                                                                       <  1:34382/149‑1 (MQ=255)
                                            ggACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCgg                                                                                                      >  8:333226/1‑149 (MQ=255)
                                            ggACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCgg                                                                                                      <  2:208000/149‑1 (MQ=255)
                                                   gACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGaaa                                                                                                 <  6:406946/147‑1 (MQ=255)
                                                    aCCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAagag                                                                                              <  4:381362/149‑1 (MQ=255)
                                                          gCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCAAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGAGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGctc                                                                                        >  3:31708/1‑149 (MQ=255)
                                                           cATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGctct                                                                                       <  7:333226/149‑1 (MQ=255)
                                                            aTCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAAccggccg                                                                                                                              >  4:301215/1‑109 (MQ=255)
                                                            aTCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAAccggccg                                                                                                                              <  3:301215/109‑1 (MQ=255)
                                                            aTCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCg                                                                                                       >  6:250202/1‑132 (MQ=255)
                                                            aTCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCg                                                                                                       <  5:250202/132‑1 (MQ=255)
                                                               aaCAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTa                                                                                   <  4:116472/149‑1 (MQ=255)
                                                               aaCAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTa                                                                                   >  7:250150/1‑149 (MQ=255)
                                                                  aGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCGACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTacga                                                                                <  2:47826/149‑1 (MQ=255)
                                                                  aGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCTGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTacga                                                                                >  5:223420/1‑149 (MQ=255)
                                                                       cgcTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAg                                                                           >  2:341569/1‑149 (MQ=255)
                                                                       cgcTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAg                                                                           >  3:372959/1‑149 (MQ=255)
                                                                        gcTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGc                                                                          >  4:84868/1‑149 (MQ=255)
                                                                             gcCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCg                                                                     <  5:5331/149‑1 (MQ=255)
                                                                               cTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAAACGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGAc                                                                   >  7:275830/1‑149 (MQ=255)
                                                                                tGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGAcc                                                                  >  3:376716/1‑149 (MQ=255)
                                                                                 gCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCAACCGGCAGTAAGCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCt                                                                 >  1:359980/1‑149 (MQ=255)
                                                                                  ccGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGcaac                                                                                                                                       <  1:156373/78‑1 (MQ=255)
                                                                                  ccGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGcaac                                                                                                                                       >  2:156373/1‑78 (MQ=255)
                                                                                  ccGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCt                                                                 <  4:357481/148‑1 (MQ=255)
                                                                                    gCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGAt                                                              >  8:106950/1‑149 (MQ=255)
                                                                                     cAACCCCTGACAATTCCTCGGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAAGCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATc                                                             <  2:413818/149‑1 (MQ=255)
                                                                                      aaCCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTTATCCGGCGAAAGAGAATCTCTATTACGACGCAGCTTTCGACCTGATCa                                                            <  2:91407/149‑1 (MQ=255)
                                                                                        ccccTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCaaa                                                          <  7:33122/149‑1 (MQ=255)
                                                                                         cccTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGCCCTGATCaaa                                                          >  4:394844/1‑148 (MQ=255)
                                                                                              aCAATTCCTCCGGTGGTGGCGCTGCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGaa                                                     <  4:31708/148‑1 (MQ=255)
                                                                                              aCAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGa                                                      <  8:385973/147‑1 (MQ=255)
                                                                                               cAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCTGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAg                                                   <  6:223420/149‑1 (MQ=255)
                                                                                                aaTTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTAtt                                                                                    >  6:59935/1‑115 (MQ=255)
                                                                                                aaTTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTAtt                                                                                    <  5:59935/115‑1 (MQ=255)
                                                                                                aaTTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGa                                                  >  1:10648/1‑149 (MQ=255)
                                                                                                    cctccGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACtt                                               <  3:430070/148‑1 (MQ=255)
                                                                                                      tccGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGa                                            >  2:340161/1‑149 (MQ=255)
                                                                                                         ggtggtGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACaa                                         <  8:250150/149‑1 (MQ=255)
                                                                                                          gtggtgGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAg                                        <  6:368776/149‑1 (MQ=255)
                                                                                                           tggtggCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGTCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAgg                                       <  3:84868/149‑1 (MQ=255)
                                                                                                                gcgcGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTTATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGgccagc                                  <  5:336077/149‑1 (MQ=255)
                                                                                                                gcgcGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGgccagc                                  >  6:338193/1‑149 (MQ=255)
                                                                                                                  gcgTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGgccagc                                  >  1:402458/1‑147 (MQ=255)
                                                                                                                   cgTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGccagccag                               >  8:200709/1‑149 (MQ=255)
                                                                                                                     tCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGc                             >  8:281396/1‑149 (MQ=255)
                                                                                                                        aaTGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGCGtt                          >  2:200455/1‑149 (MQ=255)
                                                                                                                        aaTGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGCGt                           >  1:372218/1‑148 (MQ=255)
                                                                                                                            ccgccCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGCGTTCaa                       <  2:10648/148‑1 (MQ=255)
                                                                                                                             cgccCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGCGTTCAACg                     <  7:106950/149‑1 (MQ=255)
                                                                                                                              gccCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGCGTTCAACGc                    >  6:268013/1‑149 (MQ=255)
                                                                                                                                        gcGCAGCAGCACCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTGCGACAAGGCCAGCCAGGCGTTCAACGCCTTCATgcg           >  4:299279/2‑148 (MQ=255)
                                                                                                                                        ccGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGCGTTCAACGCCTTCCTgcg           >  7:306406/1‑148 (MQ=255)
                                                                                                                                          gcagcaGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGCGTTCAACGCCTTCCTgcgc          >  7:249567/1‑147 (MQ=255)
                                                                                                                                          gcagcaGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGCGTTCAACGCCTTCCTGCGCaa        >  7:77389/1‑149 (MQ=255)
                                                                                                                                             gcagGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGCGTTCAACGCCTTCCTGCGCaa        <  8:18018/146‑1 (MQ=255)
                                                                                                                                             gcagGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGCGTTCAACGCCTTCCTGCGCaa        >  7:18018/1‑146 (MQ=255)
                                                                                                                                                ggTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGCGTTCAACGCCTTCCTGCGCAAGTAccc  >  8:212443/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AACGATGTGTCCCGCATGAAGCAGGAAAACCTGGAGCGTTACCAGGACCTGGACCGTCGCATCAACAGTGGCGCTGCGCCTGCCGCAACCCCTGACAATTCCTCCGGTGGTGGCGCGTCCAATGCCGCCCCCGATGCCGCAGCAGGTGCTGCTGCGCAACAACCGGCCGGCAGTAGCCAGCCCGGTGATCCGGCGAAAGAGAAGCTCTATTACGACGCAGCTTTCGACCTGATCAAACAGAAAGACTTCGACAAGGCCAGCCAGGCGTTCAACGCCTTCCTGCGCAAGTACCC  >  NC_002947/1400274‑1400566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑