Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,412,586	G→A	6.9%	P90P (CCG→CCA)	PP_1234 →	permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,412,586	0	G	A	6.9%	255.8 / 5.9	86	P90P (CCG→CCA)	PP_1234	permease
Reads supporting (aligned to +/- strand):  ref base G (37/43);  new base A (3/3);  total (40/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

CGGTGCTGGCGGGCATGGTGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTG  >  NC_002947/1412438‑1412727                                                                                                                                                      |                                                                                                                                               cGGTGCTGGCGGGCATGGTGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCg                                                                                                                                                <  5:209120/149‑1 (MQ=255)    tGCTGGCGGGCATGGTGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCgctg                                                                                                                                             <  7:398510/149‑1 (MQ=255)     gCTGGCGGGCATGGTCCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCgctgc                                                                                                                                            >  5:411639/1‑149 (MQ=255)      cTGGCGGGCATGGTGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCgctgct                                                                                                                                           >  8:359195/1‑149 (MQ=255)          cGGGCATGGTGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTgg                                                                                                                                       <  8:409235/149‑1 (MQ=255)             gCATGGTGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCAc                                                                                                                                    >  1:171870/1‑149 (MQ=255)              cATGGTGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTTTTCATGGGCGCAATGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCAc                                                                                                                                    >  6:24323/1‑148 (MQ=255)               aTGGTGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCAc                                                                                                                                    >  6:100539/1‑147 (MQ=255)               aTGGTGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCAc                                                                                                                                    >  3:173460/1‑147 (MQ=255)                tGGTGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAg                                                                                                                                 <  1:172513/149‑1 (MQ=255)                 ggTGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCACTGCTGTGGCACCAGc                                                                                                                                >  4:406511/1‑149 (MQ=255)                 ggTGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCACTGCTGTGGCACCAGc                                                                                                                                >  4:400164/1‑149 (MQ=255)                   tGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTg                                                                                                                              <  5:286355/149‑1 (MQ=255)                    gCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGTACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGa                                                                                                                             >  7:235064/1‑149 (MQ=255)                        gCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAg                                                                                                                                 <  4:148289/141‑1 (MQ=255)                        gCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAg                                                                                                                                 >  3:148289/1‑141 (MQ=255)                           tttCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCt                                                                                                                      >  1:276369/1‑149 (MQ=255)                                gATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTc                                                                                                                  >  4:299204/1‑148 (MQ=255)                                  tGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGTTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAAc                                                                                                               <  6:309360/149‑1 (MQ=255)                                  tGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAAc                                                                                                               <  5:100539/149‑1 (MQ=255)                                  tGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAAc                                                                                                               >  5:31128/1‑149 (MQ=255)                                  tGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAAc                                                                                                               <  3:299204/149‑1 (MQ=255)                                         gCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTAGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGc                                                                                                       <  3:372913/149‑1 (MQ=255)                                          cTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGcc                                                                                                      >  7:105614/1‑149 (MQ=255)                                             gTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGc                                                                                                                                <  1:382927/121‑1 (MQ=255)                                             gTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGc                                                                                                                                >  2:382927/1‑121 (MQ=255)                                             gTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGA‑C‑TGccactt                                                                                                  >  3:26586/1‑145 (MQ=255)                                               cAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGTGGTTCGCATTGTTCATGGGGGCACTGGCGGTGTTCATGGTGGTGATGGTACCGATGCTTTGGCACCAGGTGATCAGGGTTTTAAACGGGA‑TGCCggg                                                                                                   >  1:107899/1‑148 (MQ=255)                                                aaCGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCAt                                                                                                >  2:400195/1‑149 (MQ=255)                                                aaCGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCAt                                                                                                >  3:49430/1‑149 (MQ=255)                                                aaCGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCAt                                                                                                >  4:393102/1‑149 (MQ=255)                                                   gCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCt                                                                                             >  8:316006/1‑149 (MQ=255)                                                    cccTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTc                                                                                            <  4:55765/149‑1 (MQ=255)                                                      cTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCgg                                                                                          <  7:316006/149‑1 (MQ=255)                                                         gAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGCATGCCCGGCA‑GCTCgtt                                                                                         <  6:118970/147‑3 (MQ=255)                                                               gtgggcgtACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCa                                                                                 <  4:29174/149‑1 (MQ=255)                                                               gtgcgcgtACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGc                                                                                                                                <  3:262800/103‑1 (MQ=255)                                                               gtgcgcgtACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGc                                                                                                                                >  4:262800/1‑103 (MQ=255)                                                                   gcgtACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCg                                                                             <  4:194053/149‑1 (MQ=255)                                                                     gtACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGTCAAGTGGCAGTCgtt                                                                           >  6:300051/1‑149 (MQ=255)                                                                     gtACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCgtt                                                                           >  4:279951/1‑149 (MQ=255)                                                                        ccTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCgttgtt                                                                        <  6:441850/149‑1 (MQ=255)                                                                        ccTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCgttgtt                                                                        >  8:381040/1‑149 (MQ=255)                                                                        ccTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCgttgt                                                                         <  7:359195/148‑1 (MQ=255)                                                                        ccTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCgttgt                                                                         <  6:411639/148‑1 (MQ=255)                                                                         cTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGttgttg                                                                       <  1:400195/149‑1 (MQ=255)                                                                         cTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGttgttg                                                                       >  3:120714/1‑149 (MQ=255)                                                                         cTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGttgttg                                                                       >  1:35917/1‑149 (MQ=255)                                                                         cTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGttgttg                                                                       <  3:393102/149‑1 (MQ=255)                                                                           acacGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTtgct                                                                     >  8:352471/1‑149 (MQ=255)                                                                            cacGGCTGGCGGTGATGGTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTTGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTtgctg                                                                    <  8:105614/149‑1 (MQ=255)                                                                            cacGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTtgctg                                                                    >  4:377155/1‑149 (MQ=255)                                                                             acGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTtgctgc                                                                   <  2:35917/149‑1 (MQ=255)                                                                               ggCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTtgctgctg                                                                 <  4:120714/149‑1 (MQ=255)                                                                                    gCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTtgctgctgctgc                                                             <  4:49430/148‑1 (MQ=255)                                                                                       gTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGa                                                         <  2:360046/149‑1 (MQ=255)                                                                                        tGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCACTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAg                                                        <  3:400164/149‑1 (MQ=255)                                                                                        tGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCACTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAg                                                        <  3:406511/149‑1 (MQ=255)                                                                                             cTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTa                                                   >  6:361329/1‑149 (MQ=255)                                                                                               ggT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATc                                                 >  5:131292/1‑149 (MQ=255)                                                                                               ggT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATc                                                 <  3:279951/149‑1 (MQ=255)                                                                                                gt‑gtTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGTGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATc                                                 <  5:300051/148‑1 (MQ=255)                                                                                                    ttCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCtgt                                                                                                                                         <  8:2901/58‑1 (MQ=255)                                                                                                    ttCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCtgt                                                                                                                                         >  7:2901/1‑58 (MQ=255)                                                                                                    ttCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCa                                             >  2:307020/1‑149 (MQ=255)                                                                                                       gCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTg                                                                                    >  1:14366/1‑107 (MQ=255)                                                                                                       gCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTg                                                                                    <  2:14366/107‑1 (MQ=255)                                                                                                        ccTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTg                                         <  7:352471/149‑1 (MQ=255)                                                                                                         cTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGGATCTgg                                        >  4:282084/1‑149 (MQ=255)                                                                                                             ttCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGgtgt                                     <  6:31128/148‑1 (MQ=255)                                                                                                             ttCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTc                                    <  3:377155/149‑1 (MQ=255)                                                                                                                aTGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCg                                                           <  6:175703/123‑1 (MQ=255)                                                                                                                aTGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCg                                                           >  5:175703/1‑123 (MQ=255)                                                                                                                aTGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGa                                 <  6:277941/149‑1 (MQ=255)                                                                                                                aTGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGa                                 <  6:131292/149‑1 (MQ=255)                                                                                                                     cgcACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAg                             >  6:172455/1‑148 (MQ=255)                                                                                                                        aCTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCCGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTc                                    >  1:155750/1‑138 (MQ=255)                                                                                                                        aCTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCCGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTc                                    <  2:155750/138‑1 (MQ=255)                                                                                                                         cTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGt                        <  7:381040/149‑1 (MQ=255)                                                                                                                             cGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTg                    <  4:173460/149‑1 (MQ=255)                                                                                                                               gtgtTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCt                                          >  8:76376/1‑125 (MQ=255)                                                                                                                               gtgtTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCt                                          <  7:76376/125‑1 (MQ=255)                                                                                                                                 gtTGATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCAc                 <  5:24323/148‑1 (MQ=255)                                                                                                                                    cATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGcc              <  5:172455/148‑1 (MQ=255)                                                                                                                                     aTGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCa             <  5:36202/148‑1 (MQ=255)                                                                                                                                      tgctggtgctggTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCAtt           <  2:276369/149‑1 (MQ=255)                                                                                                                                        gatcagctggtGCCACAGCAGCGGTACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGc               <  4:121500/138‑1 (MQ=255)                                                                                                                                        gatcagctggtGCCACAGCAGCGGTACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGc               >  3:121500/6‑143 (MQ=255)                                                                                                                                              ctggtACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGt   >  6:210311/1‑149 (MQ=255)                                                                                                                                               tggtACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTg  >  3:346309/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                               CGGTGCTGGCGGGCATGGTGCTGGCGTTTCTGATGCAGGGGCTGGTCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGT‑GTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTG  >  NC_002947/1412438‑1412727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑