| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,428,978 | C→T | 7.9% | P360L (CCA→CTA) ‡ | mqo‑II → | malate:quinone oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,428,978 | 0 | C | T | 7.9% | 213.6 / 5.8 | 76 | P360L (CCA→CTA) ‡ | mqo‑II | malate:quinone oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (35/35); new base T (2/4); total (37/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01 | |||||||||||
TGCTGGACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCC > NC_002947/1428831‑1429123 | tgCTGGACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCa < 5:59183/149‑1 (MQ=255) tGGACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTg > 4:155492/1‑149 (MQ=255) aCGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTc < 2:431326/149‑1 (MQ=255) aCGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCAGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTc < 5:116469/149‑1 (MQ=255) ggCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGa < 8:211713/149‑1 (MQ=255) cAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTGGGTACTGGTCGata < 7:250576/149‑3 (MQ=255) cAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTGGGTACTGGTCGata < 2:223536/149‑3 (MQ=255) cAAGCGCGTCATCCTGTGTGGCCCGGTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTGGGTACTGGTCGata < 6:235373/149‑3 (MQ=255) cgTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCg < 5:273562/149‑1 (MQ=255) gTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGc > 6:134435/1‑149 (MQ=255) aTCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTg < 3:155492/148‑1 (MQ=255) tCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCATTACACACTGGTCGAGTA‑CCTCGCTGGc > 7:10039/1‑149 (MQ=255) tCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTGGGTACTGGTCGa < 4:152960/136‑1 (MQ=255) tCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTGGGTACTGGTCGa > 3:152960/1‑136 (MQ=255) tCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGc > 8:113467/1‑149 (MQ=255) cTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGc > 1:592/1‑147 (MQ=255) tGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAg > 4:277100/1‑149 (MQ=255) gTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAg > 4:241686/1‑148 (MQ=255) ttGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGATGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCAATGGTCGAGTA‑CCTCGCTGGCCAg > 5:196943/1‑146 (MQ=255) gCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATg > 7:40752/1‑149 (MQ=255) cccGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTg < 8:8283/137‑1 (MQ=255) cccGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTg > 7:8283/1‑137 (MQ=255) cccGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGc > 6:41776/1‑149 (MQ=255) ccGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCt > 6:1882/1‑149 (MQ=255) gTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGt < 4:99426/149‑1 (MQ=255) cGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCt > 5:61548/1‑148 (MQ=255) cGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCt < 4:407622/148‑1 (MQ=255) gCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGa < 5:41776/149‑1 (MQ=255) gCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGa < 3:241686/149‑1 (MQ=255) tctcGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGAc > 2:247246/1‑149 (MQ=255) gACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTACCCTCGCTGGCCAGCTGATGCTGTCTGAc < 1:26622/140‑1 (MQ=255) gACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGAc > 2:26622/1‑139 (MQ=255) aacAAGTTCCTGAAGAACGGCTCGTAACTGGACCTGCTTAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTTGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCtt < 2:355838/147‑1 (MQ=255) aCCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCtt < 6:61548/149‑1 (MQ=255) aCCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACACACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCGGATGGTGTCTGACGATGCCCGCtg > 4:344762/1‑148 (MQ=255) cAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGGCTGACGATGACCGCTTc > 5:101130/1‑148 (MQ=255) aGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGaa < 7:113467/149‑1 (MQ=255) aGTTCCTGAAGAACGGCTAGTAACTTGACCTGCTGATGAGCACCAACACCCACAACGTGTGGCCGATGAACAAAGTCGGTCCATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCg < 1:348641/149‑1 (MQ=255) gTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAg < 7:354150/149‑1 (MQ=255) gTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTAACCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAg > 1:343532/1‑149 (MQ=255) tGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTg > 7:365333/1‑149 (MQ=255) aaCGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCAc < 2:592/149‑1 (MQ=255) gCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTAc < 1:287737/149‑1 (MQ=255) cTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACt < 5:134435/149‑1 (MQ=255) gTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACtt < 1:406295/147‑1 (MQ=255) cTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGAc > 1:5495/1‑117 (MQ=255) cTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGAc < 2:5495/117‑1 (MQ=255) cTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGaa > 3:298283/1‑149 (MQ=255) aCCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGcc < 8:40752/149‑1 (MQ=255) ctgctgAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCaa < 8:10039/149‑1 (MQ=255) gcagcaCCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAagaaagaa < 2:280389/149‑1 (MQ=255) cagcaCCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCaagaaag > 8:148294/1‑146 (MQ=255) cagcaCCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCaagaaag < 7:148294/146‑1 (MQ=255) cagcaCCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAaagaag < 4:298283/149‑1 (MQ=255) caccaccacCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCACAACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGCCCGCTTCGAAGCCCTGCGCACCTACTTACCGACCGCCAAGAAAGAAGAAt > 7:105103/1‑149 (MQ=255) caccaccCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCg < 8:358120/104‑1 (MQ=255) caccaccCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCg > 7:358120/1‑104 (MQ=255) ccacccacAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGgcgc > 6:15650/1‑149 (MQ=255) ccacccacAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGgcgc > 4:87556/1‑149 (MQ=255) caACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAAGGCCAAGAAAGAAGAATGGCGCCTGTGGc < 1:247246/149‑1 (MQ=255) aaCGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTgg > 3:381984/1‑147 (MQ=255) cGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAgg > 5:432879/1‑149 (MQ=255) tgtgGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAggcc > 6:71027/1‑149 (MQ=255) ccGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAggccggc > 4:273776/1‑147 (MQ=255) cAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGAACGCTTCGAAGCCCTGCGCACCTACTTCCCGCACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCTGCCAGCGCGTg > 7:389015/1‑148 (MQ=255) aaaGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCt > 2:255767/1‑125 (MQ=255) aaaGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCt < 1:255767/125‑1 (MQ=255) aaGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCa > 2:197396/1‑148 (MQ=255) ggT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatca < 5:15650/148‑1 (MQ=255) gT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCtt < 1:378414/67‑1 (MQ=255) gT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCtt > 2:378414/1‑67 (MQ=255) tCGACCAGTGGGTACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCaaa > 3:194207/1‑149 (MQ=255) tCGACCAGTACCCAGTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGTCAGGCCGGCCAGCGCGTGCAAATCATCaaa < 6:196943/149‑1 (MQ=255) tCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCaaa < 3:277100/149‑1 (MQ=255) gACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACg > 1:294524/1‑149 (MQ=255) aGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGAt < 8:365333/149‑1 (MQ=255) ccACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCaag > 7:15632/1‑87 (MQ=255) ccACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCaag < 8:15632/87‑1 (MQ=255) ccACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGcc > 2:186862/1‑147 (MQ=255) cACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGcc > 5:51757/1‑146 (MQ=255) | TGCTGGACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGT‑‑ATCGACCAGTACCCACTGGTCGAGTA‑CCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCC > NC_002947/1428831‑1429123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |