| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,464,200 | T→C | 8.8% | Q473R (CAG→CGG) | algX ← | alginate biosynthesis protein AlgX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,464,200 | 0 | T | C | 8.8% | 207.5 / 9.0 | 84 | Q473R (CAG→CGG) | algX | alginate biosynthesis protein AlgX |
| Reads supporting (aligned to +/- strand): ref base T (42/31); new base C (3/4); total (46/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01 | |||||||||||
GAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTCG > NC_002947/1464053‑1464346 | gAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCt < 5:203734/148‑1 (MQ=255) gCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AAATCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGACTGCGCGGTCTgcg > 4:320824/1‑149 (MQ=255) cTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTgcgc < 1:327424/149‑1 (MQ=255) tCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAgg < 4:152850/148‑1 (MQ=255) tCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCAGACCGCGCAgg < 4:215477/148‑1 (MQ=255) cGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCTCCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCAGACGGCGCAGGc < 6:26789/148‑1 (MQ=255) cGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCAGACCGCGCAGGc < 3:57244/148‑1 (MQ=255) aTCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACg > 5:422905/1‑149 (MQ=255) aTCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACg < 6:235106/149‑1 (MQ=255) tCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCAGACCGCGCAGGCaggc < 1:192733/149‑4 (MQ=255) cccTGGTCTTAACCGTGGGGCGGTACCAGCGCGGCGTGCGCGGCACCACAAAACAAGGCCAACGCGAGCAGGGCGGGGCTGTA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACgg < 8:171846/147‑1 (MQ=255) cccTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCTGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGAGGTCTGCGCAGGCACGGc > 6:387004/1‑148 (MQ=255) cccTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTGGAATAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGc > 6:393606/1‑148 (MQ=255) ccTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCg > 4:422322/1‑148 (MQ=255) cTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCAGACCGCGCAGGCaggcagat > 3:227850/1‑142 (MQ=255) cTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCAGACCGCGCAGGCagg < 4:227850/144‑3 (MQ=255) agtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAgttgt > 8:102686/1‑149 (MQ=255) agtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAgttgt > 7:244891/1‑149 (MQ=255) agtaACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAgttgt > 4:358388/1‑149 (MQ=255) aCCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGACGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGGTGTTTCg > 6:232827/1‑149 (MQ=255) tGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTg > 4:302153/1‑149 (MQ=255) gggggcaCCTGCGCGGGGTGCGCGGCACCACAAAAAAAGCCCACCGCGCGCTGGGGGGGGGTGTA‑‑ATTTGTTTTCAATAGGTTCTTGGTTCGCCTCACTGGCCTGCCGGGGGGGTCGGCCCTGGCACGGCGAAGTTGTTGCGTTTGCag < 4:182149/143‑1 (MQ=255) tACCAGCGCGGCGGGCGCGGCACCACCAAACAAGCCCAACGCGCCCCGGGCGAGGCTGTA‑‑AATTCGTTTGAAACGGGTCAGGGGGCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCCGGCACGGCGAAGTTGTTGCGTTTGCAGAGCtt < 4:255332/149‑1 (MQ=255) aCCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTg > 7:342415/1‑149 (MQ=255) ccAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGGGCAGGCACGGCGGAGTTGTTGGGGTTTGAGAGCTTgg > 2:435685/1‑149 (MQ=255) aGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGAATAATTGGTTGGAAAAGGGGCATGGGGCGCCTCAATGGCATGCCTGCGCGGTGTGCGCAGGCACGGGGAAGTTGTTGCGTTTGCAGGGGTTgg > 1:120342/1‑149 (MQ=255) gcgcGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCtt < 3:302153/149‑1 (MQ=255) gcgcGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCtt > 3:106595/1‑149 (MQ=255) cggggcgTGCGGGGCACCACAAAAAAAGGCCAACGCGAGCTGGGGGGGGCTGTA‑‑ATTTCGTTTTAAACGGGTCAGGGGTCGCCTCAGTGGCCTGGCTGCGCGGTCTGCGCTGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTc < 2:261454/146‑1 (MQ=255) ggcgTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGAc < 1:43782/149‑1 (MQ=255) gcggGGAACCACCAAACTTGGCAACCGGTAGCTGGTGGGGGGTGTA‑‑ATTGCTTTTTAAATGGGTCTGGGGGGGCCTCCGTGGCCTGCGGGGGCGGTCTGCGCTGCCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCt < 6:10768/149‑1 (MQ=255) gcgGCACCACCAACCACGGCCACCGCGCGCCGGGCGGGGCGGGA‑‑AATTCGTTTGAAACGGGGCATGGGGCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTgg < 8:342415/149‑1 (MQ=255) gcgGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTgg > 2:102142/1‑149 (MQ=255) gcgGCACCACCAAACAAGGCAAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTgg < 1:322038/149‑1 (MQ=255) caccacCAAACACGCCCAACGCGAGCTGGGGGGGGGTGTA‑‑AATTCTTTTTAAACGGGGCTTGGGTCGCCTCACTGGCCTGCCGGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGc < 3:422322/149‑1 (MQ=255) caccacCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGttt < 1:267211/120‑1 (MQ=255) caccacCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGttt > 2:267211/1‑120 (MQ=255) caccacCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGc > 7:31912/1‑149 (MQ=255) caccacCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAACTTGGCTTCGACCTTCTGGGTGc > 1:11105/1‑149 (MQ=255) accaccAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGcc > 6:14838/1‑149 (MQ=255) ccaAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCtt > 1:48996/1‑149 (MQ=255) caAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCtt > 4:381377/1‑148 (MQ=255) aCAAGGCCACCGCTTGTAGGGGGGGTGTGTA‑‑AATTCTTTTGAAAAGGTTATTGGGTCGCCTCAGTGGCCTGCCTGCGCGTTCTGCGATGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCg < 8:408377/149‑1 (MQ=255) acgcccAACGCGCGCAGGGCGGGGCTGGA‑‑AATTCGTTTCAAACGGGTCATGGGGCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGc < 7:68170/145‑1 (MQ=255) ggCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGccc > 3:195117/1‑149 (MQ=255) gCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGcccc > 5:413746/1‑149 (MQ=255) cAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTg > 5:142749/1‑149 (MQ=255) aGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTc > 7:124931/1‑148 (MQ=255) cAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGaa > 3:46862/1‑149 (MQ=255) gCGGGGCTGGA‑‑AATTCGTTTCAAACGGGGCAGGGGGCGCCGCACGGGCCGGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAAcgcg < 6:92704/149‑1 (MQ=255) cGGGGCTGTA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGa < 6:142749/149‑1 (MQ=255) cGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGa < 6:422905/149‑1 (MQ=255) cGGGGCTGGA‑‑AATGCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGa < 1:59607/149‑1 (MQ=255) cTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGaa > 4:369921/1‑139 (MQ=255) cTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGaa < 3:369921/139‑1 (MQ=255) tGGA‑‑AATACGTTTG‑AAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCAGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCATCGACCTTCTGGGTTCCGCTATCAGGCCCCTGTACCTAGAACGCCAGCAGgcg > 7:240006/1‑149 (MQ=255) taatttCGTTTTAAACGGGTCTTGGGGCGCCTCACTGGCCTGCCTGCGGGGTCGGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGgcgc < 8:244891/145‑1 (MQ=255) aTTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCgctgg > 6:388120/1‑149 (MQ=255) gTTTGAAACGGGTCATGGGCCGCCTCACTGCCCGGCCTGCGCGGGCTCCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCGTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCgctggctg < 7:404570/148‑1 (MQ=255) tttGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCtggctggc > 5:204117/1‑149 (MQ=255) ccgggTCATGGGTCGCCTCACTGGCCGGCCTGCGCGGGCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTc < 3:320824/147‑1 (MQ=255) acgggTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTATGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTc < 2:120342/147‑1 (MQ=255) ggTCTTGGGGCGCGTCACTGCCCGGCCTGCGCGGTGTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTc < 4:46862/149‑1 (MQ=255) tCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGt > 1:316092/1‑149 (MQ=255) gggTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTc > 1:369318/1‑149 (MQ=255) gggTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAACAGGCGCTGGCTGGCCCAGTCCTCGTCTTc > 2:306817/1‑149 (MQ=255) ggTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTcg > 8:342548/1‑149 (MQ=255) ggTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAACTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTc < 2:11105/148‑1 (MQ=255) gCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCa > 4:103659/1‑149 (MQ=255) tCACTGGCCTGCCAGCGCGGGATGCGCAGGCACGGCGAAGTTGTTGCGGTTTCAGCGCTTGGCTTAGCCCTTGTGGGTGCCGCTTTACGGGCCCGGTTCCGAGAAACCGCGCAAGCGCGGGCTGGCCCAGTCCACGGGGTGGCGCATCt > 2:423852/1‑149 (MQ=255) tGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTgg > 6:109515/1‑147 (MQ=255) gcctgcctGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCgctgg < 8:300322/115‑1 (MQ=255) gcctgcctGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCgctgg > 7:300322/1‑115 (MQ=255) gcctgcctGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTcgc > 3:402362/1‑137 (MQ=255) gcctgcctGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTcgc < 4:402362/137‑1 (MQ=255) gcctgcctGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGa > 7:219000/1‑149 (MQ=255) gcctgcctGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGa > 2:19113/1‑149 (MQ=255) cgtgcctgcGCAGACCGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGAc > 1:69658/3‑149 (MQ=255) gtgcctgcGCAGCCCGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGGCGCTTTCCGGCCCGTGGACGTGGGGCGCGAGCAGGCGGGGGGGGGCCCAGTCCTCGTCGTGGCGCCTGTggtggccg > 4:51209/2‑143 (MQ=255) ctgcGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGc > 8:408454/1‑149 (MQ=255) ggcTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTc < 7:102686/147‑1 (MQ=255) gTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTc > 3:220037/1‑149 (MQ=255) gTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTc < 3:358388/149‑1 (MQ=255) gTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGATTTCCGTACCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGt < 2:48996/148‑1 (MQ=255) tCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTCg < 6:413746/149‑1 (MQ=255) tGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTc < 6:445049/113‑1 (MQ=255) tGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTc > 5:445049/1‑113 (MQ=255) | GAGCTTTTCGATCCCCTGGTAGTAACCCTGCGGCGGTACCAGCGCGGCGTGCGCGGCACCACCAAACAAGGCCAACGCGAGCAGGGCGGGGCTGGA‑‑AATTCGTTTGAAAAGGGTCATGGGTCGCCTCACTGGCCTGCCTGCGCGGTCTGCGCAGGCACGGCGAAGTTGTTGCGTTTGCAGAGCTTGGCTTCGACCTTCTGGGTGCCGCTTTCCGGCCCCTGTACCTCGAACGCGAGCAGGCGCTGGCTGGCCCAGTCCTCGTCTTCGCGCATCTGGAAGACGAAGCGGCCGTCG > NC_002947/1464053‑1464346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |