Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,553,213 G→T 6.6% R85L (CGC→CTC)  PP_1363 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,553,2130GT 6.6% 265.6 / 4.6 84R85L (CGC→CTC) PP_1363hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (33/45);  new base T (2/4);  total (35/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACCATCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGTGGC  >  NC_002947/1553067‑1553357
                                                                                                                                                  |                                                                                                                                                
ccaccaTCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAgcgc                                                                                                                                                 >  3:178136/1‑148 (MQ=255)
 caccaTCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGCGCTcc                                                                                                                                                <  3:392312/148‑1 (MQ=255)
 caccaTCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGCGCTcc                                                                                                                                                >  4:392312/1‑148 (MQ=255)
 caccaTCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAgcgc                                                                                                                                                 >  6:87482/1‑147 (MQ=255)
 caccaTCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAgcgc                                                                                                                                                 >  6:271540/1‑147 (MQ=255)
    caTCTGAACACTGCCTACCGCGCCTACGACCGCGGCGATTGCGCAGGGGGCATGCGCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGc                                                                                                                                            <  4:290576/149‑1 (MQ=255)
    caTCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGc                                                                                                                                            <  3:5364/149‑1 (MQ=255)
       cTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGtt                                                                                                                                         >  1:2835/1‑149 (MQ=255)
          aaCAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGCGCTCCAGGcac                                                                                                                                          <  8:119442/145‑3 (MQ=255)
          aaCAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGtt                                                                                                                                         <  8:209846/146‑1 (MQ=255)
          aaCAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGtt                                                                                                                                         <  3:179448/146‑1 (MQ=255)
          aaCAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGt                                                                                                                                          <  2:73556/145‑1 (MQ=255)
          aaCAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTa                                                                                                                                      <  6:310420/149‑1 (MQ=255)
           aCAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTAc                                                                                                                                     >  3:210749/1‑149 (MQ=255)
           aCAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTAc                                                                                                                                     <  2:29375/149‑1 (MQ=255)
              aTGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTg                                                                                                                                  >  5:13653/1‑149 (MQ=255)
              aTGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTg                                                                                                                                  <  1:198457/149‑1 (MQ=255)
               tGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTg                                                                                                                                  >  3:51826/1‑148 (MQ=255)
                 ccTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGa                                                                                                                                <  3:237454/148‑1 (MQ=255)
                 ccTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGAc                                                                                                                               >  6:191123/1‑149 (MQ=255)
                   tACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGAcgc                                                                                                                             >  5:59660/1‑149 (MQ=255)
                   tACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGAcgc                                                                                                                             >  2:81641/1‑149 (MQ=255)
                       gcgcCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTg                                                                                                                                  <  5:390374/140‑1 (MQ=255)
                       gcgcCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTg                                                                                                                                  >  6:390374/1‑140 (MQ=255)
                           cTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCaaa                                                                                                                     >  2:46290/1‑149 (MQ=255)
                              cGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAAc                                                                                                                    <  6:404220/147‑1 (MQ=255)
                              cGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTGGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAAc                                                                                                                    <  3:288982/147‑1 (MQ=255)
                                aCCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTAt                                                                                                                <  5:191123/149‑1 (MQ=255)
                                    cggcgATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAg                                                                                                             >  5:247065/1‑148 (MQ=255)
                                      gcgATTGCGCACGGGTCATGCTCGAACTGTCGCAGGGTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTAc                                                                                                          <  8:75984/149‑1 (MQ=255)
                                       cgATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTAcc                                                                                                         <  1:81641/149‑1 (MQ=255)
                                       cgATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTAcc                                                                                                         <  1:94301/149‑1 (MQ=255)
                                        gATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCt                                                                                                        >  3:180227/1‑149 (MQ=255)
                                         aTTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCtt                                                                                                       <  4:180227/149‑1 (MQ=255)
                                          ttGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTa                                                                                                      <  7:140319/149‑1 (MQ=255)
                                             cgcACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATc                                                                                                    >  8:294173/1‑148 (MQ=255)
                                             cgcACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATc                                                                                                    >  3:301475/1‑148 (MQ=255)
                                                  gggTCATGCTCGAACTGTCGCAAGTTGATCGCACCAGACGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGATGTACGTGGACGCGGCGCAAACCTATCAGTACCTTAACcag                                                                                                 >  5:121990/1‑146 (MQ=255)
                                                        tGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCg                                                                                        <  4:301475/149‑1 (MQ=255)
                                                           tCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCggg                                                                                      >  1:191053/1‑148 (MQ=255)
                                                               aCTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAAc                                                                                  >  4:205225/1‑148 (MQ=255)
                                                                   tCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAata                                                                             >  5:365044/1‑149 (MQ=255)
                                                                    cGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAatat                                                                            >  5:358251/1‑149 (MQ=255)
                                                                     gCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATg                                                                           >  3:140394/1‑149 (MQ=255)
                                                                      cAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGc                                                                          <  6:247065/149‑1 (MQ=255)
                                                                      cAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGACACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGc                                                                          >  1:320683/1‑149 (MQ=255)
                                                                             aTCGCAGCAGCCGTGCCCGGCCGTTCATCCACGCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATcgc                                                                   <  6:121990/149‑1 (MQ=255)
                                                                              tCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATcgcg                                                                  >  2:238098/1‑149 (MQ=255)
                                                                              tCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATcgcg                                                                  >  3:230111/1‑149 (MQ=255)
                                                                                gCACCAGCCGTGCCCGTCCGTTCATCCACCCCGAAGTTTCACTGCTGCGCGGCCAGTGCCTTGAGCGCCAGGCGTTGTACGTGGACGCTGCTCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCgcgc                                                                 <  6:221279/148‑1 (MQ=255)
                                                                                 cACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATc                                                                     >  1:431842/1‑143 (MQ=255)
                                                                                 cACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATc                                                                     <  2:431842/143‑1 (MQ=255)
                                                                                 cACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGccc                                                               >  8:370550/1‑149 (MQ=255)
                                                                                       ccGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGgcgc                                                         <  2:23793/149‑1 (MQ=255)
                                                                                         gTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGgcgcgc                                                       <  7:370550/149‑1 (MQ=255)
                                                                                          tGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCACTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGCGCGCGcc                                                      >  4:251553/1‑149 (MQ=255)
                                                                                            cccGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTg                                                    <  4:210749/149‑1 (MQ=255)
                                                                                              cGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCa                                                  <  6:59660/149‑1 (MQ=255)
                                                                                                   gTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGAccc                                             >  1:406366/1‑149 (MQ=255)
                                                                                                        tCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGaa                                        <  3:167519/149‑1 (MQ=255)
                                                                                                           aCCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGaaaaa                                     >  3:263559/1‑149 (MQ=255)
                                                                                                           aCCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGaaaaa                                     <  1:238098/149‑1 (MQ=255)
                                                                                                               cGAAGTCTCACTGCTGCGCGGCCAGTGCCTGGAGCTCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGATTATTCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCg                                 <  6:43075/149‑1 (MQ=255)
                                                                                                                  aGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTc                              <  1:46290/149‑1 (MQ=255)
                                                                                                                      tCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCAttt                          <  5:87482/149‑1 (MQ=255)
                                                                                                                        aCTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTgc                        >  3:194281/1‑149 (MQ=255)
                                                                                                                        aCTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTgc                        >  2:271235/1‑149 (MQ=255)
                                                                                                                          tgctgcGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTgcgc                      >  1:835/1‑149 (MQ=255)
                                                                                                                          tgctgcGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTgcgc                      >  4:82145/1‑149 (MQ=255)
                                                                                                                            ctgcGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCATTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGcgcg                     <  2:320683/148‑1 (MQ=255)
                                                                                                                            ctgcGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCgg                    <  4:140394/149‑1 (MQ=255)
                                                                                                                               cgcgGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGt                   <  6:358251/147‑1 (MQ=255)
                                                                                                                               cgcgGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGCACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGc                 <  4:51826/149‑1 (MQ=255)
                                                                                                                                    ccAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGaaa            >  2:208767/1‑149 (MQ=255)
                                                                                                                                     caacgCCTGGCGCTCCAGGCGTTGTACGTGGATGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAAc           >  2:418479/5‑149 (MQ=255)
                                                                                                                                     cAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAAc           <  6:13653/149‑1 (MQ=255)
                                                                                                                                     cAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAAc           >  3:407359/1‑149 (MQ=255)
                                                                                                                                       gTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACgg         <  5:271540/149‑1 (MQ=255)
                                                                                                                                       gTGCCTGGAGCGCCAGGCGTTGTAAGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACgg         <  4:178136/149‑1 (MQ=255)
                                                                                                                                         gCCTGGCGCTCCAGGCGTTGTACGTGGATGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGcc       <  1:418479/149‑1 (MQ=255)
                                                                                                                                           cTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGt     <  2:191053/149‑1 (MQ=255)
                                                                                                                                           cTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACCCGGTCATTTGCGCGGTGCAGAAACGGCCGt     <  7:326493/149‑1 (MQ=255)
                                                                                                                                             ggAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGTgg   <  4:230111/149‑1 (MQ=255)
                                                                                                                                              gAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGTgg   <  7:294173/148‑1 (MQ=255)
                                                                                                                                              gAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGTgg   <  3:82145/148‑1 (MQ=255)
                                                                                                                                              gAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGTg    <  4:263559/147‑1 (MQ=255)
                                                                                                                                              gAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGTGGc  <  6:365044/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CCACCATCTGAACAATGCCTACCGCGCCTACGACCGCGGCGATTGCGCACGGGTCATGCTCGAACTGTCGCAGGTTGATCGCACCAGCCGTGCCCGGCCGTTCATCCACCCCGAAGTGTCACTGCTGCGCGGCCAGTGCCTGGAGCGCCAGGCGTTGTACGTGGACGCGGCGCAAACCTATCAGTACCTTATCCAGCAGTACCCGGGCAACGAATATGCCTATCGCGCCCGGGCGCGCCTGCAGACCCTGGAAAAACTCGGTCATTTGCGCGGTGCAGAAACGGCCGTGGC  >  NC_002947/1553067‑1553357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑