| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 196,327 | A→C | 16.5% | T611T (ACA→ACC) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 196,327 | 0 | A | C | 16.5% | 353.9 / 61.0 | 157 | T611T (ACA→ACC) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base A (63/68); new base C (13/13); total (76/81) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
Only 100 of 162 total aligned reads displayed.
ACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAG > NC_002947/196181‑196474 | aCCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑Ca < 2:314373/147‑1 (MQ=38)aCCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGt > 7:227212/1‑149 (MQ=39) cccGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGa > 8:128085/1‑149 (MQ=39) cccGAACCCGACGCCGGCTCAGACCACGATAAACGACTCGGTCGACACCACTACCGCCACCCTGACGGCGACCCCCTCGGTCACCGAACGCGGCGTGATCACTTACACCCTGACCCTGACCAATCCTGCCCAGACGCCGGA‑GA‑CAGTGa > 7:166897/1‑149 (MQ=14) cGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGAcc > 5:105858/1‑149 (MQ=39) aaCCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCt < 3:426462/149‑1 (MQ=39) ccGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTc < 5:9280/149‑1 (MQ=39) ccGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTc > 1:74891/1‑149 (MQ=39) ccGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACgg < 2:253233/149‑1 (MQ=39) cGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGTTGGA‑CAGGGTCACTGTcaccggc > 2:269539/1‑143 (MQ=11) cGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGc > 7:242022/1‑149 (MQ=39) ggCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGc > 1:223072/1‑148 (MQ=39) gCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGcc > 6:19675/1‑148 (MQ=39) cTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCaa < 3:179744/149‑1 (MQ=39) tCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCaaa > 7:218008/1‑149 (MQ=39) gACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCa > 5:346577/1‑149 (MQ=39) aCCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCAt < 8:18104/149‑1 (MQ=39) aCCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCAt < 4:352517/149‑1 (MQ=39) aCCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCAt > 4:232056/1‑149 (MQ=39) cACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTa > 1:161205/1‑149 (MQ=39) cGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTAcc < 7:296084/149‑1 (MQ=39) gATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCg > 3:301423/1‑149 (MQ=39) gATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCg > 8:372667/1‑149 (MQ=39) aTCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGt < 5:190520/149‑1 (MQ=39) aaCGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTg < 7:372667/148‑1 (MQ=39) aCGTCTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACAACGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGaa < 3:246927/149‑1 (MQ=255) cGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAg > 1:260638/1‑149 (MQ=39) cGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAg > 7:327923/1‑149 (MQ=39) aCTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGcc > 5:310270/1‑149 (MQ=39) cTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCg > 5:149319/1‑149 (MQ=39) cTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCg < 2:260638/149‑1 (MQ=39) ggTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑AA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGc > 2:331816/1‑148 (MQ=255) cGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCAGCCCAGACGCCGGT‑GA‑CAGTGACCCTCTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCaaaa > 3:102040/1‑149 (MQ=255) gACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAAc < 1:277487/149‑1 (MQ=40) accacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAAcc > 5:161948/1‑147 (MQ=38) accacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAAcc > 6:39114/1‑147 (MQ=38) cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGc > 4:292597/1‑135 (MQ=34) cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGc < 3:292597/135‑1 (MQ=34) cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCa > 7:393882/1‑140 (MQ=34) cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCa < 8:393882/140‑1 (MQ=34) cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAgg > 2:46013/1‑149 (MQ=37) acTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAggg < 3:126638/149‑1 (MQ=17) acTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAggg < 8:301536/149‑1 (MQ=37) acTACCGCGACCATGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAggg > 6:423186/1‑149 (MQ=37) ccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGc > 3:237856/1‑149 (MQ=34) cgcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCg < 3:38212/149‑1 (MQ=34) cgACCCTGACGGCGAGCCCGTCAGTCACCGAAGGCGGTGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTc < 2:433758/149‑1 (MQ=21) cccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGc > 3:71129/1‑128 (MQ=32) cccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGc < 4:71129/128‑1 (MQ=32) ccTGACGGCGAGCCCGTCGTTCACCGAAGGCGGCGTGATCACTTAAACCGTGACACTGACAAACCCTGCCCAGACGACGGT‑GA‑AAGTGAAACTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAACCCCAGGGCAGCGTCgcct > 6:137572/1‑146 (MQ=11) ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGAGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGAtt > 4:407362/1‑149 (MQ=21) cTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGActt > 7:58921/1‑149 (MQ=255) tGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCAA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAAcc > 5:382623/1‑131 (MQ=255) aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTcc < 8:218008/148‑1 (MQ=34) aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTcc < 8:330991/148‑1 (MQ=34) aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTc < 6:150050/147‑1 (MQ=34) aCGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCa < 2:56069/149‑1 (MQ=34) cGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑TA‑CAGTGACCCTGTCCAGTCGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTcc < 1:331816/148‑1 (MQ=255) cGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCa < 6:346577/148‑1 (MQ=34) cccGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACGGTTGAAGCCGGCAACACCCAGGGCAGCGTCGATTTCCAGACCCCgg > 6:106893/1‑149 (MQ=17) ggTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAAt < 1:113223/148‑1 (MQ=255) gTCACCGAAGGCGGCGTGATCACTTACACCGTGCCCCTGAGCACTCCTGCCCAGACGCCGGT‑GA‑CAGTGCCCCTGTCCA‑CCGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGa > 5:272241/1‑149 (MQ=255) gTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCATGGCAGCGTCGATTTCCAGACCCCGGCGAATGa < 5:423186/149‑1 (MQ=21) gTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGa > 7:19727/1‑149 (MQ=34) tCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGAc < 7:55011/149‑1 (MQ=34) tCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGAc > 5:287083/1‑149 (MQ=255) aCCGAAGGCGGCGTGATCACTTACACCGTGTCCCTGAGCAATCCTGCCCAGACGCGGGT‑GACCAGT‑CCCGCGCCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGt < 1:142653/149‑1 (MQ=11) ccGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTc < 6:161948/149‑1 (MQ=34) ccGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTc < 8:384193/149‑1 (MQ=34) cGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCt < 7:163639/149‑1 (MQ=255) cGAAGGCGGCGTCATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑Ac < 3:244296/76‑1 (MQ=21) aaGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTa > 7:243845/1‑148 (MQ=34) aaGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTac < 6:287083/149‑1 (MQ=255) gcggcgTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTacaa > 3:18715/1‑148 (MQ=255) cggcgTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTacaac > 1:174480/1‑148 (MQ=255) ggcgTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTcc > 8:237492/1‑121 (MQ=35) ggcgTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTAcaacaa < 6:265151/149‑1 (MQ=34) tGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCgg < 3:319105/126‑1 (MQ=255) tGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCgg > 4:319105/1‑126 (MQ=255) acacCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGt < 8:19727/148‑1 (MQ=34) acacCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGt < 4:295017/148‑1 (MQ=34) acacCGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGt < 8:58921/148‑1 (MQ=255) acacCGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGt > 3:208928/1‑148 (MQ=255) cacCTTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAATCCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCa < 6:272241/149‑1 (MQ=21) acCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAg < 2:161205/149‑1 (MQ=34) ccGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAgc < 6:82464/149‑2 (MQ=255) cGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAgtg < 7:128085/149‑1 (MQ=35) cGTGACCCTGAGCAACCCTGCCCAGACGCCGGT‑GA‑CCGTGACCCTGTCCA‑ACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAgcg > 8:321801/1‑147 (MQ=255) tGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACt > 1:416789/1‑140 (MQ=33) tGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACt < 2:416789/140‑1 (MQ=33) aCCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCATGGCAGCGGCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCa < 8:327923/148‑1 (MQ=255) cTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCCCCAt > 5:337644/1‑149 (MQ=37) tCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACg > 6:165948/1‑149 (MQ=37) cTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGc < 4:237856/148‑1 (MQ=37) ccAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACcg < 3:344160/149‑1 (MQ=38) aGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACcggc > 4:260646/1‑149 (MQ=39) aGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACcggc > 6:413400/1‑149 (MQ=39) gCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCgag > 2:212032/1‑133 (MQ=37) t‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTc < 8:111988/149‑1 (MQ=39) aGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg > 7:115284/1‑148 (MQ=38) | ACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGT‑GA‑CAGTGACCCTGTCCA‑ACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAG > NC_002947/196181‑196474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |